##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921501_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2046099 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.99622061298109 31.0 30.0 34.0 22.0 34.0 2 29.110219984468003 31.0 30.0 34.0 22.0 34.0 3 29.193415372374456 31.0 30.0 34.0 22.0 34.0 4 32.554335347409875 37.0 33.0 37.0 23.0 37.0 5 32.54600290601774 37.0 33.0 37.0 23.0 37.0 6 32.564147678093775 37.0 33.0 37.0 23.0 37.0 7 32.392216114665025 37.0 33.0 37.0 21.0 37.0 8 32.3274685144756 37.0 33.0 37.0 19.0 37.0 9 33.719933883942076 39.0 34.0 39.0 18.0 39.0 10-11 33.5771365412915 39.0 34.0 39.0 17.5 39.0 12-13 33.409858222891465 38.0 34.0 39.0 17.0 39.0 14-15 34.4569014011541 39.0 34.0 41.0 17.0 41.0 16-17 34.354064001790725 39.0 34.0 41.0 17.0 41.0 18-19 34.361331734192724 39.0 34.0 41.0 17.5 41.0 20-21 34.36664916995707 39.0 34.0 41.0 18.0 41.0 22-23 34.31090504418408 39.0 34.0 41.0 17.0 41.0 24-25 34.25184949506353 39.0 34.0 41.0 17.0 41.0 26-27 34.1480563745938 39.0 33.5 41.0 16.0 41.0 28-29 34.08475494098771 39.0 33.0 41.0 16.0 41.0 30-31 33.97606836228354 39.0 33.0 41.0 15.0 41.0 32-33 33.85416077130188 39.0 33.0 41.0 12.0 41.0 34-35 33.68127446423658 39.0 33.0 41.0 9.0 41.0 36-37 33.53443601702557 39.0 33.0 41.0 9.0 41.0 38-39 33.403014468019386 39.0 33.0 41.0 8.0 41.0 40-41 33.24149271369567 38.0 32.5 41.0 7.5 41.0 42-43 33.046475512670696 38.0 32.0 41.0 2.0 41.0 44-45 32.85161568428507 38.0 32.0 40.0 2.0 41.0 46-47 32.599168710800406 38.0 32.0 40.0 2.0 41.0 48-49 32.465422005484584 38.0 31.5 40.0 2.0 41.0 50-51 32.27528995420065 38.0 31.0 40.0 2.0 41.0 52-53 32.06987882795505 37.0 31.0 40.0 2.0 41.0 54-55 31.855532650179683 37.0 31.0 40.0 2.0 41.0 56-57 31.611419584291866 36.5 31.0 40.0 2.0 41.0 58-59 31.363334569832645 36.0 30.0 40.0 2.0 41.0 60-61 31.057693200573382 35.5 30.0 39.5 2.0 41.0 62-63 30.740057788015143 35.0 30.0 39.0 2.0 41.0 64-65 30.435615041109937 35.0 29.0 39.0 2.0 40.5 66-67 30.39641947921386 35.0 30.0 38.5 2.0 40.0 68-69 30.161454797641756 35.0 30.0 37.5 2.0 40.0 70-71 29.86620686486822 35.0 29.0 37.0 2.0 39.5 72-73 29.548908190659397 35.0 29.0 36.5 2.0 39.0 74-75 29.259276799411957 35.0 29.0 36.0 2.0 39.0 76-77 28.99623356445607 35.0 29.0 36.0 2.0 38.0 78-79 28.72748532695632 34.0 29.0 35.0 2.0 37.0 80-81 28.492071498006695 34.0 29.0 35.0 2.0 37.0 82-83 28.301861737872898 34.0 29.0 35.0 2.0 36.0 84-85 28.107263871396253 34.0 28.0 35.0 2.0 36.0 86-87 27.957721742691824 34.0 28.0 35.0 2.0 36.0 88-89 27.834539286710957 34.0 28.0 35.0 2.0 35.0 90-91 27.724952702679587 34.0 27.0 35.0 2.0 35.0 92-93 27.623135293062553 34.0 27.0 35.0 2.0 35.0 94-95 27.55136237298391 34.0 27.0 35.0 2.0 35.0 96-97 27.451517497442694 34.0 27.0 35.0 2.0 35.0 98-99 27.388666188683928 34.0 27.0 35.0 2.0 35.0 100-101 27.321950697400275 34.0 27.0 35.0 2.0 35.0 102-103 26.361393803525637 32.5 24.5 34.5 2.0 35.0 104-105 27.171493656954038 34.0 26.5 35.0 2.0 35.0 106-107 27.496604025513918 34.0 27.0 35.0 2.0 35.0 108-109 27.53513784034888 34.0 27.0 35.0 2.0 35.0 110-111 27.473047003102 34.0 27.0 35.0 2.0 35.0 112-113 27.397437269653132 34.0 27.0 35.0 2.0 35.0 114-115 27.270459054034042 34.0 27.0 35.0 2.0 35.0 116-117 27.19834059837769 34.0 27.0 35.0 2.0 35.0 118-119 27.041097718145604 34.0 27.0 35.0 2.0 35.0 120-121 26.86049111015645 34.0 26.0 35.0 2.0 35.0 122-123 26.72662613099366 34.0 25.5 35.0 2.0 35.0 124-125 25.87723370179058 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115316.0 3 19194.0 4 12463.0 5 10461.0 6 10774.0 7 12187.0 8 13277.0 9 13579.0 10 13893.0 11 14179.0 12 14199.0 13 14362.0 14 14973.0 15 12545.0 16 9958.0 17 8987.0 18 8123.0 19 7527.0 20 7379.0 21 7882.0 22 9314.0 23 12797.0 24 17595.0 25 23559.0 26 27981.0 27 30414.0 28 35224.0 29 41158.0 30 47070.0 31 56467.0 32 69895.0 33 89623.0 34 123107.0 35 188285.0 36 344669.0 37 469155.0 38 118454.0 39 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.62806533115354 17.24617884716171 12.283226820816482 24.842529000868264 2 16.693090304309376 19.27102048039689 38.477167386044705 25.558721829249038 3 19.189532591122706 24.21502499864337 28.648195976457718 27.947246433776208 4 13.35104783649204 14.98451406934079 35.66863286244045 35.99580523172673 5 14.441650027281423 37.35393683617653 33.22173048302783 14.982682653514217 6 33.755934268668774 34.459122796637644 17.19831618106188 14.586626753631707 7 29.972727663868703 31.134515493111998 20.62087834719278 18.27187849582652 8 27.78380223020992 33.54462375474731 19.403207637940948 19.268366377101824 9 27.882650417219928 14.60069305246196 18.765950082231537 38.750706448086575 10-11 26.395666089832176 24.86942998012329 26.854558748503017 21.880345181541525 12-13 26.671464495779368 22.59427941992877 26.9191703503939 23.81508573389796 14-15 24.14693023466156 23.772234424444207 24.253046786863646 27.827788554030587 16-17 24.36820205604612 26.671225010493398 24.706019825128763 24.254553108331724 18-19 24.355461845052663 25.854237680978002 26.232782365778366 23.55751810819097 20-21 25.50375120610287 24.869046448837032 25.894276678164076 23.73292566689603 22-23 25.277225672321002 24.48221826504762 25.707309642609754 24.53324642002162 24-25 24.71111997953741 24.961400337554476 25.75144018189403 24.576039501014083 26-27 24.370778448735543 25.02282910771745 25.90132495618156 24.705067487365447 28-29 24.417790728121908 25.03754749516705 25.980201718615415 24.564460058095626 30-31 24.82005453130073 24.946578688979905 25.692154377564712 24.541212402154652 32-33 24.452394295667958 25.10271885889886 25.709349396694805 24.735537448738377 34-35 24.739814557672524 25.086687736398876 25.83451003093965 24.33898767498895 36-37 24.893061902702286 24.84506659318633 25.59678120911505 24.665090294996336 38-39 24.81190542270388 25.099319854312135 25.594678162591865 24.49409656039212 40-41 24.854194135786546 24.90582976525103 25.814929787034814 24.42504631192761 42-43 24.751035274648533 24.992713466101478 25.645200570403563 24.61105068884642 44-45 24.63242800342222 25.014310190151313 25.635229401945303 24.71803240448116 46-47 24.80045071899363 25.08126977947732 25.455817458807577 24.66246204272147 48-49 24.898910292190145 25.194251889709474 25.22926258239554 24.677575235704843 50-51 24.71370245397198 25.35136989332438 25.20939164017767 24.725536012525968 52-53 24.913396578018638 25.151169338757473 25.38992018571901 24.54551389750488 54-55 24.772004148446314 25.135897800746466 25.409258115605915 24.682839935201308 56-57 24.725119978094675 25.308520339434715 25.184300533208813 24.782059149261794 58-59 24.868363631029435 25.09147985022902 25.248120833837582 24.79203568490396 60-61 24.8325315692323 25.229058287545307 25.386119144777354 24.552290998445038 62-63 24.70493810771675 25.33639023736723 25.334165523139106 24.62450613177691 64-65 24.75170478729061 25.019185198097105 25.451710874725244 24.777399139887045 66-67 24.63805776408387 25.283031690357756 25.28818972168143 24.790720823876946 68-69 24.75658820892286 25.22601955996555 25.239170677798345 24.778221553313237 70-71 24.627728513752622 24.982659318504492 25.538636896520444 24.85097527122244 72-73 24.89257282791046 25.289281531668617 25.19859268510119 24.619552955319733 74-75 24.6317745740921 25.440991644780574 25.257346478924198 24.66988730220313 76-77 24.62773565252932 25.0955609882853 25.342942762653074 24.933760596532302 78-79 24.68447089060476 25.138339998244852 25.34690418343284 24.83028492771755 80-81 24.666443097707617 25.39099717784927 25.120294602618397 24.822265121824714 82-83 24.772501302716005 25.277505394173023 25.199635728342894 24.750357574768078 84-85 24.59063352694061 25.280341121618676 25.16998888412013 24.959036467320583 86-87 24.42865016332661 25.4074610869621 25.29759904387027 24.86628970584102 88-89 24.59028454356537 25.21339423071753 25.242725091133426 24.953596134583673 90-91 24.48076568700818 25.15693973504398 25.37125173949788 24.991042838449953 92-93 24.66047090948286 25.363506201541675 25.23171763615504 24.744305252820425 94-95 24.556752582106842 25.077842028340854 25.346781959223975 25.01862343032833 96-97 24.55631063168149 25.062699885671385 25.392561650203316 24.98842783244381 98-99 24.448007155836173 25.12101745823624 25.284589901912746 25.146385484014843 100-101 24.369052504030677 25.207326071065555 25.4121532070807 25.01146821782306 102-103 24.489272242729943 25.01171200563056 25.50695205592437 24.992063695715128 104-105 24.25470185019232 25.25532429135916 25.374142860389647 25.115830998058875 106-107 24.29933721748651 25.198321537499023 25.39879369672323 25.103547548291232 108-109 24.328997621268737 25.160392670291426 25.362876883976917 25.147732824462928 110-111 24.266106411087936 25.244590465847267 25.400970981504628 25.088332141560173 112-113 24.296208606669857 25.23162375762884 25.478918822749385 24.99324881295192 114-115 24.17607420421556 25.407695261035183 25.4264887484686 24.989741786280657 116-117 24.11661733766089 25.440154763798738 25.424685226689814 25.018542671850554 118-119 24.27263822512974 25.016307022221397 25.417399758046166 25.293654994602697 120-121 23.873380020553885 25.181060312731958 25.69927227670209 25.246287390012068 122-123 23.9969348172012 25.214966109442184 25.608380121776243 25.179718951580377 124-125 23.921645485852142 25.3087925225094 25.555342677589916 25.21421931404855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 144.0 1 148.5 2 163.0 3 177.0 4 168.0 5 159.0 6 167.0 7 191.0 8 235.0 9 286.5 10 320.0 11 347.5 12 400.0 13 470.0 14 579.0 15 674.0 16 750.0 17 850.0 18 937.5 19 1011.0 20 1120.0 21 1254.5 22 1501.5 23 1853.0 24 2261.0 25 2951.5 26 4264.5 27 5789.5 28 6777.0 29 8298.5 30 11006.5 31 13707.0 32 16932.5 33 20415.0 34 23898.5 35 27576.0 36 32141.0 37 36541.0 38 38604.0 39 41033.0 40 43862.0 41 46153.0 42 48905.5 43 54094.0 44 59436.0 45 63263.0 46 67582.5 47 71371.0 48 76794.5 49 82443.5 50 83352.5 51 83219.5 52 83940.0 53 85292.0 54 87052.5 55 86958.5 56 83272.5 57 76673.5 58 72088.0 59 67190.0 60 60049.0 61 53018.5 62 45937.0 63 36587.5 64 27423.5 65 21293.5 66 16053.5 67 11828.0 68 9147.5 69 7297.5 70 6250.0 71 5172.5 72 4086.5 73 3736.5 74 3064.0 75 2224.0 76 1523.5 77 874.0 78 517.0 79 363.5 80 203.0 81 88.5 82 59.5 83 33.0 84 29.0 85 29.0 86 22.0 87 19.0 88 14.5 89 14.5 90 13.0 91 8.0 92 10.5 93 14.0 94 11.0 95 9.0 96 10.0 97 9.0 98 7.0 99 10.0 100 34.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031425654379382426 2 0.028444371460031995 3 0.02873761240291892 4 0.033918202393921314 5 0.03670399135134712 6 0.04818926161441846 7 0.039245412856367165 8 0.03572652154172403 9 0.032012136265156275 10-11 0.036190819701295 12-13 0.0356532113060023 14-15 0.034529121024935744 16-17 0.03741265696332387 18-19 0.050339695195589264 20-21 0.06304680272068947 22-23 0.056180077308087245 24-25 0.06803189874976724 26-27 0.049484409112169066 28-29 0.04997314401698061 30-31 0.05058406264799504 32-33 0.05850156810594209 34-35 0.04041837662791488 36-37 0.05412739070787875 38-39 0.06434195021844007 40-41 0.048849053735914055 42-43 0.06016326678230134 44-45 0.0599922095656173 46-47 0.06676118799725722 48-49 0.0493377886407256 50-51 0.05217245108863256 52-53 0.0630223659754489 54-55 0.04867799651923001 56-57 0.04564784010939842 58-59 0.047724963454847495 60-61 0.034871235458303826 62-63 0.043619590254430504 64-65 0.04410832515924205 66-67 0.0364107504084602 68-69 0.03154783810558531 70-71 0.05143934873141525 72-73 0.031450091124623 74-75 0.04156690365422201 76-77 0.03311178980098226 78-79 0.0335027777248315 80-81 0.024241251278652697 82-83 0.018229811949470674 84-85 0.018425305911395292 86-87 0.01717903190412585 88-89 0.023068287507104983 90-91 0.012218372620288655 92-93 0.01945164921149954 94-95 0.014124438749053687 96-97 0.01182738469643942 98-99 0.010581110689169976 100-101 0.011118719084462679 102-103 0.005938129093460288 104-105 0.005987002583941442 106-107 0.008748354796126678 108-109 0.012731544270340782 110-111 0.006255806781587792 112-113 0.007941942203187627 114-115 0.008675044560404946 116-117 0.0069644723935645345 118-119 0.2346171910547828 120-121 0.3454622674660415 122-123 0.026587178821748117 124-125 0.0031767768812750504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2046099.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.5664322302319 #Duplication Level Percentage of deduplicated Percentage of total 1 80.35790425354975 44.652020408683356 2 9.909757373751 11.012997230531516 3 3.4587767424595373 5.765756503781404 4 1.683898190676385 3.7427285877931786 5 1.0159911602716203 2.822750197687383 6 0.6694210551426081 2.231840381644324 7 0.47590517996749915 1.851104705147625 8 0.35094840048022014 1.560076040927395 9 0.2840281374207449 1.4204187224521931 >10 1.6459528481991066 16.79043211071906 >50 0.10609231213753768 3.977537996742427 >100 0.04026077708164379 3.7139279872363007 >500 7.976766468009335E-4 0.26495929323136896 >1k 2.6589221560030805E-4 0.19344983342245484 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.8873490481154624E-5 0.0 0.0 0.0 3 0.0 4.8873490481154624E-5 0.0 0.0 0.0 4 0.0 4.8873490481154624E-5 0.0 0.0 0.0 5 0.0 4.8873490481154624E-5 0.0 0.0 0.0 6 0.0 4.8873490481154624E-5 0.0 0.0 0.0 7 0.0 4.8873490481154624E-5 0.0 0.0 0.0 8 0.0 4.8873490481154624E-5 0.0 0.0 0.0 9 0.0 4.8873490481154624E-5 0.0 0.0 0.0 10-11 0.0 4.8873490481154624E-5 0.0 4.8873490481154624E-5 0.0 12-13 0.0 4.8873490481154624E-5 0.0 4.8873490481154624E-5 0.0 14-15 0.0 4.8873490481154624E-5 0.0 4.8873490481154624E-5 0.0 16-17 0.0 4.8873490481154624E-5 0.0 4.8873490481154624E-5 0.0 18-19 0.0 4.8873490481154624E-5 0.0 4.8873490481154624E-5 0.0 20-21 0.0 4.8873490481154624E-5 0.0 4.8873490481154624E-5 4.8873490481154624E-5 22-23 0.0 4.8873490481154624E-5 0.0 1.466204714434639E-4 4.8873490481154624E-5 24-25 0.0 4.8873490481154624E-5 0.0 1.954939619246185E-4 4.8873490481154624E-5 26-27 0.0 4.8873490481154624E-5 0.0 4.887349048115462E-4 4.8873490481154624E-5 28-29 0.0 4.8873490481154624E-5 0.0 0.0011485270263071337 4.8873490481154624E-5 30-31 0.0 4.8873490481154624E-5 0.0 0.003983189474214102 4.8873490481154624E-5 32-33 0.0 4.8873490481154624E-5 0.0 0.010165686020080163 4.8873490481154624E-5 34-35 0.0 4.8873490481154624E-5 0.0 0.017594456573215667 4.8873490481154624E-5 36-37 0.0 4.8873490481154624E-5 0.0 0.029592898486339125 4.8873490481154624E-5 38-39 0.0 4.8873490481154624E-5 0.0 0.048653559773989434 9.774698096230925E-5 40-41 0.0 4.8873490481154624E-5 0.0 0.06974247091660765 1.466204714434639E-4 42-43 0.0 4.8873490481154624E-5 0.0 0.09156448441644319 1.466204714434639E-4 44-45 0.0 4.8873490481154624E-5 0.0 0.11766292833337977 1.710572166840412E-4 46-47 0.0 4.8873490481154624E-5 0.0 0.14600955281244943 1.954939619246185E-4 48-49 0.0 4.8873490481154624E-5 0.0 0.17645773738220877 1.954939619246185E-4 50-51 0.0 4.8873490481154624E-5 0.0 0.21040037652137067 2.443674524057731E-4 52-53 0.0 4.8873490481154624E-5 0.0 0.24485618731058467 3.6655117860865966E-4 54-55 0.0 4.8873490481154624E-5 0.0 0.281144753992842 4.1542466908981433E-4 56-57 0.0 4.8873490481154624E-5 0.0 0.32236954321369593 4.3986141433039166E-4 58-59 0.0 4.8873490481154624E-5 0.0 0.37055880482811443 4.3986141433039166E-4 60-61 0.0 4.8873490481154624E-5 0.0 0.42243801497386 4.6429815957096894E-4 62-63 0.0 4.8873490481154624E-5 0.0 0.477420691765159 4.887349048115462E-4 64-65 0.0 4.8873490481154624E-5 0.0 0.5325499890279013 4.887349048115462E-4 66-67 0.0 4.8873490481154624E-5 0.0 0.5944482647222837 5.376083952927009E-4 68-69 0.0 4.8873490481154624E-5 0.0 0.6670987083225202 5.864818857738555E-4 70-71 0.0 4.8873490481154624E-5 0.0 0.749255045821341 6.353553762550101E-4 72-73 0.0 4.8873490481154624E-5 0.0 0.8347103439276399 7.575391024578968E-4 74-75 0.0 4.8873490481154624E-5 0.0 0.9175264735479565 8.308493381796287E-4 76-77 0.0 4.8873490481154624E-5 0.0 1.0130497106933731 8.308493381796287E-4 78-79 0.0 4.8873490481154624E-5 0.0 1.1226240763521218 8.308493381796287E-4 80-81 0.0 4.8873490481154624E-5 0.0 1.2492552901887934 8.308493381796287E-4 82-83 0.0 4.8873490481154624E-5 0.0 1.3860766267907856 8.797228286607833E-4 84-85 0.0 7.331023572173194E-5 0.0 1.5243886048524533 8.797228286607833E-4 86-87 0.0 9.774698096230925E-5 0.0 1.6692496306385958 8.797228286607833E-4 88-89 0.0 9.774698096230925E-5 0.0 1.8343687182291766 8.797228286607833E-4 90-91 0.0 9.774698096230925E-5 0.0 2.018597340597889 8.797228286607833E-4 92-93 0.0 9.774698096230925E-5 0.0 2.214946588605928 9.041595739013605E-4 94-95 0.0 9.774698096230925E-5 0.0 2.426642112625049 9.774698096230924E-4 96-97 0.0 9.774698096230925E-5 0.0 2.649383045492911 9.774698096230924E-4 98-99 0.0 9.774698096230925E-5 0.0 2.885490878007369 9.774698096230924E-4 100-101 0.0 9.774698096230925E-5 0.0 3.1509961150462416 9.774698096230924E-4 102-103 0.0 9.774698096230925E-5 0.0 3.4456543891571227 9.774698096230924E-4 104-105 0.0 9.774698096230925E-5 0.0 3.7563676048910635 9.774698096230924E-4 106-107 0.0 9.774698096230925E-5 0.0 4.074973889337711 9.774698096230924E-4 108-109 0.0 9.774698096230925E-5 0.0 4.407997853476298 0.001026343300104247 110-111 0.0 9.774698096230925E-5 0.0 4.772423035249028 0.0010752167905854018 112-113 0.0 9.774698096230925E-5 0.0 5.160136435236026 0.0010752167905854018 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 67.82017 1 GTATCAA 4050 0.0 57.45832 1 TCAACGC 5320 0.0 42.49663 4 CAACGCA 5410 0.0 41.67969 5 AACGCAG 5790 0.0 38.83863 6 ATCAACG 5960 0.0 38.13195 3 GTACATG 10335 0.0 38.064682 1 TACATGG 10780 0.0 35.430523 2 TATCAAC 6470 0.0 35.217278 2 ACATGGG 10925 0.0 34.79776 3 ACGCAGA 6770 0.0 32.864994 7 CATGGGG 8905 0.0 31.468027 4 CGCAGAG 7265 0.0 30.377855 8 GCAGAGT 7955 0.0 26.769508 9 GTATAGG 810 0.0 25.71664 1 ATGGGGG 5005 0.0 25.557413 5 AGAGTAC 7095 0.0 25.195105 10-11 CAGAGTA 7655 0.0 23.585094 10-11 GTATACG 180 2.1736982E-5 23.144978 1 TATAGGA 1145 0.0 21.822529 2 >>END_MODULE