FastQCFastQC Report
Mon 15 Aug 2016
SRR2921501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046099
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25130.12281908157914159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21020.10273207699138702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.078.785511
GTATCAA56700.058.6586381
TCAACGC75250.043.3672144
CAACGCA76750.042.5691155
AACGCAG80700.040.5473446
ATCAACG82000.040.015223
TATCAAC84000.038.9915852
GTACATG108900.035.248351
ACGCAGA95050.034.298927
CGCAGAG100200.032.5938348
TACATGG117250.032.454542
ACATGGG116450.031.6547323
CATGGGG90250.029.4949594
GCAGAGT107800.029.3570949
ATGGGGG48250.026.8880835
CAGAGTA103100.026.02279510-11
AGAGTAC98300.025.84106810-11
GAGTACT51000.024.1463912-13
GTATAGG7800.022.9249021
CATGGGT22450.022.8123264
Produced by FastQC (version 0.11.5)