##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2046099
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16296376666036	31.0	31.0	34.0	28.0	34.0
2	31.214539961165123	33.0	31.0	34.0	28.0	34.0
3	31.304810764288533	33.0	31.0	34.0	28.0	34.0
4	34.837615872936745	37.0	35.0	37.0	32.0	37.0
5	34.484060155447025	37.0	35.0	37.0	30.0	37.0
6	34.459765143328845	37.0	35.0	37.0	30.0	37.0
7	34.296619078548986	37.0	35.0	37.0	30.0	37.0
8	34.25793424462844	37.0	35.0	37.0	30.0	37.0
9	35.82705871025791	39.0	37.0	39.0	30.0	39.0
10-11	35.69076398551585	39.0	35.0	39.0	30.0	39.0
12-13	35.61108577835188	39.0	35.0	39.0	30.0	39.0
14-15	36.773770721749045	40.0	37.0	41.0	30.0	41.0
16-17	36.74427557024367	40.0	36.5	41.0	30.0	41.0
18-19	36.75382031856718	40.0	37.0	41.0	30.0	41.0
20-21	36.73155111262945	40.0	37.0	41.0	30.0	41.0
22-23	36.66823355077149	40.0	36.5	41.0	30.0	41.0
24-25	36.60485734072496	40.0	36.0	41.0	29.5	41.0
26-27	36.48129147221127	40.0	36.0	41.0	29.0	41.0
28-29	36.29056414181328	40.0	36.0	41.0	28.5	41.0
30-31	36.249130174053164	40.0	36.0	41.0	29.0	41.0
32-33	36.13645796220027	40.0	35.0	41.0	28.0	41.0
34-35	36.02399517325408	40.0	35.0	41.0	28.0	41.0
36-37	35.9008923810627	40.0	35.0	41.0	27.0	41.0
38-39	35.73302415963255	40.0	35.0	41.0	27.0	41.0
40-41	35.531300782611204	39.0	35.0	41.0	26.0	41.0
42-43	35.36841057055402	39.0	35.0	41.0	26.0	41.0
44-45	35.16883616090912	39.0	35.0	41.0	25.0	41.0
46-47	34.917829000454034	39.0	34.5	41.0	24.0	41.0
48-49	34.68549126899529	39.0	34.0	41.0	23.0	41.0
50-51	34.47186597520452	39.0	34.0	41.0	23.0	41.0
52-53	34.2707139292869	38.0	34.0	40.5	22.5	41.0
54-55	34.05196229507956	38.0	33.5	40.0	22.0	41.0
56-57	33.79279179550941	37.5	33.0	40.0	21.0	41.0
58-59	33.642032961259446	37.0	33.0	40.0	21.5	41.0
60-61	33.52119692155658	37.0	33.0	40.0	21.0	41.0
62-63	33.24508564834839	36.0	33.0	40.0	20.0	41.0
64-65	32.94459896613019	36.0	33.0	39.0	20.0	41.0
66-67	32.64158112583995	35.0	33.0	39.0	19.5	41.0
68-69	32.32163155350743	35.0	32.0	38.5	18.0	40.5
70-71	32.01820146532499	35.0	32.0	37.5	18.0	40.0
72-73	31.708525589426515	35.0	32.0	37.0	17.5	39.0
74-75	31.414521975720625	35.0	32.0	36.5	15.5	39.0
76-77	31.130007883294013	35.0	32.0	36.0	13.5	39.0
78-79	30.855479133707604	35.0	31.0	35.5	11.5	37.0
80-81	30.634096639507668	35.0	31.0	35.0	10.5	37.0
82-83	30.45283879225785	35.0	31.0	35.0	9.0	36.5
84-85	30.263710846835856	35.0	31.0	35.0	7.0	36.0
86-87	30.12464914943021	35.0	31.0	35.0	7.0	36.0
88-89	30.0222652960585	35.0	31.0	35.0	7.0	36.0
90-91	29.92701917160411	35.0	31.0	35.0	7.0	35.0
92-93	29.989813787113917	35.0	31.0	35.0	5.5	35.0
94-95	29.955948123722266	35.0	31.0	35.0	2.0	35.0
96-97	29.933458498342457	35.0	31.0	35.0	2.0	35.0
98-99	29.89649083450996	35.0	31.0	35.0	2.0	35.0
100-101	29.84721829197903	35.0	31.0	35.0	2.0	35.0
102-103	28.950275377682118	33.5	29.5	35.0	2.0	35.0
104-105	29.665116643916058	34.5	31.0	35.0	2.0	35.0
106-107	29.776406713458147	35.0	31.0	35.0	2.0	35.0
108-109	29.78295062946612	35.0	31.0	35.0	2.0	35.0
110-111	29.75096146374149	35.0	31.0	35.0	2.0	35.0
112-113	29.699515272721406	35.0	31.0	35.0	2.0	35.0
114-115	29.633364025885356	35.0	31.0	35.0	2.0	35.0
116-117	29.55345073723217	35.0	31.0	35.0	2.0	35.0
118-119	29.460977205892775	35.0	31.0	35.0	2.0	35.0
120-121	29.333049378353635	35.0	31.0	35.0	2.0	35.0
122-123	29.20924598467621	35.0	31.0	35.0	2.0	35.0
124-125	28.287977023594657	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35588.0
3	12717.0
4	7270.0
5	6085.0
6	6500.0
7	7639.0
8	8786.0
9	9598.0
10	10950.0
11	11450.0
12	12165.0
13	13561.0
14	16438.0
15	13476.0
16	7991.0
17	6988.0
18	6378.0
19	5855.0
20	5658.0
21	6292.0
22	7302.0
23	10151.0
24	13759.0
25	18766.0
26	23352.0
27	27254.0
28	32038.0
29	38073.0
30	44516.0
31	53616.0
32	66707.0
33	85079.0
34	118261.0
35	183728.0
36	362058.0
37	573175.0
38	176593.0
39	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60099800653137	17.868065063214583	12.49445850952745	25.036478420726592
2	16.985800975144375	19.66177856324558	37.67829424023585	25.674126221374195
3	19.182814685323233	24.341576852249723	28.894699715047906	27.58090874737914
4	13.482620807891236	15.494011500227629	35.365669390216055	35.65769830166508
5	14.433292088465107	37.29261254055841	33.24447190843151	15.029623462544983
6	33.789255457011414	34.87276302005552	16.672622332721147	14.665359190211916
7	29.764205681392692	31.443087821383287	20.40766148230052	18.385045014923506
8	27.90093310217985	33.56860874372119	19.108384234185138	19.42207391991382
9	27.864528984852033	14.396556822352894	18.72007906092667	39.01883513186841
10-11	26.5320930250745	24.835760331543938	26.67779600897972	21.954350634401838
12-13	26.824410501190982	22.45575688912469	27.042079495248405	23.677753114435923
14-15	24.297810196145896	23.534013219760432	24.498760525271404	27.669416058822264
16-17	24.29942926913554	26.474844266700163	24.959192486578548	24.266533977585745
18-19	24.271432296497014	25.43063030566962	26.889293095472176	23.408644302361186
20-21	25.355922874843557	24.405630244933203	26.67848249389555	23.559964386327692
22-23	25.094511941045177	24.053334819227963	26.49318358057105	24.35896965915581
24-25	24.507084593529466	24.658564751168548	26.537695903216935	24.29665475208505
26-27	24.190138745377354	24.638302052977995	26.706212308874406	24.465346892770246
28-29	24.364739103720623	24.69499900528543	26.723679045934308	24.21658284505964
30-31	24.689961493581695	24.595809707801937	26.436633550592898	24.277595248023474
32-33	24.24460088790694	24.763927466825688	26.476343866586667	24.5151277786807
34-35	24.507470646709745	24.788849648239587	26.54627073831924	24.15740896673143
36-37	24.697593752535735	24.55305075184846	26.284218367959454	24.46513712765635
38-39	24.635168564894105	24.803233614574243	26.229146771677176	24.332451048854477
40-41	24.681542750084436	24.662895508483153	26.36416914196783	24.291392599464583
42-43	24.593632684662484	24.726556972561973	26.176577904654746	24.503232438120797
44-45	24.549585550760195	24.73785602868809	26.051081107107642	24.66147731344407
46-47	24.72704307088277	24.880410256610798	25.76370748246115	24.628839190045284
48-49	24.864021939317354	25.081484179175977	25.356507796450263	24.697986085056407
50-51	24.722404711347824	25.235117685933606	25.335812578454032	24.706665024264538
52-53	24.962132516759585	25.10751627684607	25.39632840011136	24.534022806282984
54-55	24.700223514071364	25.10487074923041	25.518096818621206	24.676808918077022
56-57	24.690403876715898	25.168638412552692	25.31873747646835	24.822220234263064
58-59	24.78455771487342	24.906738829862245	25.527103114848536	24.781600340415796
60-61	24.805546736120636	25.080419803472846	25.580817839032317	24.533215621374204
62-63	24.681667960893098	25.217470370280747	25.522218531415973	24.578643137410182
64-65	24.714769234379034	24.89111370515498	25.643492339097584	24.750624721368403
66-67	24.699264391941085	25.187812353591415	25.40051034810081	24.71241290636669
68-69	24.844304359037974	25.049185376745687	25.323068913301988	24.78344135091435
70-71	24.59230948209789	24.85281983701178	25.68890190205065	24.865968778839687
72-73	24.903902439620524	25.158117668337237	25.29943796950444	24.6385419225378
74-75	24.65728581264575	25.369489974416965	25.240927089935116	24.732297123002173
76-77	24.64737890197681	25.030916668214537	25.337346064320354	24.984358365488298
78-79	24.724963663376936	25.075887686712555	25.338494038251596	24.860654611658912
80-81	24.67589763644807	25.315376530687068	25.130691956101973	24.87803387676289
82-83	24.762367938061942	25.219864950401167	25.195083862232792	24.8226832493041
84-85	24.597431804689872	25.247264745640347	25.11057229241774	25.04473115725204
86-87	24.39780597766727	25.36337914437235	25.30719172131234	24.93162315664804
88-89	24.51861090885449	25.12650510089978	25.25734252641618	25.097541463829547
90-91	24.450469685283	25.059584385469424	25.385025598312282	25.104920330935293
92-93	24.589379489405296	25.292898870341478	25.271905951724595	24.845815688528628
94-95	24.501016772034117	25.023345066352288	25.31871117149099	25.156926990122614
96-97	24.495414547935617	25.022995685315408	25.324798781644116	25.156790985104855
98-99	24.387407993900474	25.04863004994966	25.254806799409597	25.30915515674027
100-101	24.308539433213618	25.14069455832772	25.363195667289478	25.18757034116918
102-103	24.449151134102042	24.93839270702592	25.475678682166	25.13677747670604
104-105	24.25718221533413	25.121313403160784	25.270552654427924	25.35095172707716
106-107	24.309074452029623	25.05336992985666	25.291848476317824	25.345707141795888
108-109	24.339216120000536	24.990571491073162	25.225581506190554	25.444630882735748
110-111	24.29824027650808	25.117168685518738	25.274835374911692	25.309755663061495
112-113	24.31056250357421	25.115364481376716	25.326493335229806	25.24757967981927
114-115	24.10515470770049	25.27589719127373	25.317978233202282	25.300969867823497
116-117	24.156004817383245	25.314723323055986	25.19822339441739	25.33104846514338
118-119	24.134608146854138	24.964709804124748	25.2475934725846	25.65308857643651
120-121	23.847735157237437	25.065468760233312	25.4828168331204	25.603979249408848
122-123	23.9179898220384	25.115348195030762	25.3834696707741	25.58319231215674
124-125	23.896481009378597	25.21911503386512	25.265775818030274	25.618628138726006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	31.5
2	64.5
3	100.0
4	82.0
5	69.0
6	121.5
7	265.5
8	461.0
9	541.0
10	432.0
11	351.0
12	357.5
13	409.0
14	514.5
15	613.0
16	737.5
17	894.5
18	1070.5
19	1238.0
20	1401.5
21	1651.5
22	1919.0
23	2175.0
24	2575.0
25	3309.0
26	4609.0
27	6124.5
28	7194.5
29	8817.5
30	11512.5
31	13960.0
32	17206.5
33	20714.0
34	23756.5
35	27129.0
36	31602.0
37	35972.5
38	38024.5
39	40483.0
40	43376.0
41	45385.5
42	48012.0
43	53378.5
44	58780.5
45	62910.5
46	67728.0
47	71988.5
48	77746.0
49	83294.5
50	83522.5
51	82991.5
52	83939.0
53	85551.0
54	87614.0
55	87619.5
56	83936.5
57	77473.5
58	73041.5
59	68328.0
60	60600.0
61	52928.5
62	45576.5
63	36050.5
64	26948.0
65	20604.0
66	15308.5
67	11209.5
68	8641.0
69	6804.0
70	5847.5
71	4971.5
72	3957.5
73	3616.5
74	2872.0
75	2027.5
76	1356.5
77	755.5
78	422.5
79	239.5
80	121.5
81	48.5
82	27.5
83	13.0
84	9.5
85	6.0
86	4.0
87	3.5
88	1.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11851821441679997
2	0.0021015600906896486
3	0.02404575731672808
4	0.05356534556734547
5	0.02893310636484354
6	0.02091785392593418
7	0.016274872330224493
8	0.006255806781587792
9	0.0040076262194546795
10-11	0.007624264515060122
12-13	0.005009532774318349
14-15	0.0034700178241619784
16-17	0.010434490217726513
18-19	0.005644888150573359
20-21	0.012487176817935007
22-23	0.00945702040810342
24-25	0.0140022550228508
26-27	0.009432583662862842
28-29	0.013977818277610224
30-31	0.022775046564218054
32-33	0.013513520118039253
34-35	0.02245736887609055
36-37	0.01666586025407373
38-39	0.022603989347534013
40-41	0.010752167905854019
42-43	0.009652514370028039
44-45	0.01818093845898952
46-47	0.017350089120809892
48-49	0.02231074840464709
50-51	0.01539514950156371
52-53	0.023385965195232488
54-55	0.01791213426134317
56-57	0.021993070716519583
58-59	0.017863260770862015
60-61	0.020893417180693603
62-63	0.023239344723789027
64-65	0.01891404081620684
66-67	0.011754074460717688
68-69	0.025487525285922137
70-71	0.01500416157771447
72-73	0.019133971523372036
74-75	0.01991594737107051
76-77	0.013537956863279833
78-79	0.03137678088890127
80-81	0.021944197226038426
82-83	0.008992722248532451
84-85	0.012975911722746555
86-87	0.013073658703708862
88-89	0.021040037652137068
90-91	0.012755981015581357
92-93	0.008357366872277441
94-95	0.007990815693668782
96-97	0.0030790299003127415
98-99	0.0019060661287650304
100-101	0.013024785213227707
102-103	0.003665511786086597
104-105	0.002712478721704082
106-107	1.466204714434639E-4
108-109	0.021577646047429767
110-111	5.131716500521235E-4
112-113	0.009383710172381689
114-115	0.0022970540526142673
116-117	0.008552860834202059
118-119	0.010019065548636697
120-121	0.004154246690898143
122-123	5.620451405332782E-4
124-125	0.02365476939287884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2046099.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.56331095767953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.27532275174741	41.144101301128266
2	11.120399580132457	11.690500422083023
3	3.7401776658209416	5.897883650565424
4	1.8277189912397562	3.842838467191666
5	1.0674323688091574	2.80538897640041
6	0.7321112668570354	2.3089315305256237
7	0.5064216381597965	1.863343862960359
8	0.39651148980708023	1.6673565389617866
9	0.3064523364475238	1.4497334508958757
>10	1.8522377697971215	17.96242714734821
>50	0.1281276871958072	4.594031952088712
>100	0.04615258572002456	4.230558178744405
>500	5.603209595698316E-4	0.18908392543411584
>1k	3.7354730637988745E-4	0.35382059567214597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2513	0.12281908157914159	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2102	0.10273207699138702	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.774698096230925E-5
2	0.0	0.0	0.0	0.0	9.774698096230925E-5
3	0.0	0.0	0.0	0.0	9.774698096230925E-5
4	0.0	0.0	0.0	0.0	9.774698096230925E-5
5	0.0	0.0	0.0	0.0	9.774698096230925E-5
6	0.0	0.0	0.0	0.0	1.954939619246185E-4
7	0.0	0.0	0.0	4.8873490481154624E-5	1.954939619246185E-4
8	0.0	0.0	0.0	4.8873490481154624E-5	1.954939619246185E-4
9	0.0	0.0	0.0	4.8873490481154624E-5	1.954939619246185E-4
10-11	0.0	0.0	0.0	4.8873490481154624E-5	2.443674524057731E-4
12-13	0.0	0.0	0.0	4.8873490481154624E-5	2.443674524057731E-4
14-15	0.0	0.0	0.0	4.8873490481154624E-5	3.176776881275051E-4
16-17	0.0	0.0	0.0	4.8873490481154624E-5	3.90987923849237E-4
18-19	0.0	0.0	0.0	4.8873490481154624E-5	4.3986141433039166E-4
20-21	0.0	0.0	0.0	7.331023572173194E-5	4.3986141433039166E-4
22-23	0.0	0.0	0.0	1.466204714434639E-4	4.887349048115462E-4
24-25	0.0	0.0	0.0	2.199307071651958E-4	5.620451405332782E-4
26-27	0.0	0.0	0.0	5.376083952927009E-4	5.864818857738555E-4
28-29	0.0	0.0	0.0	0.0012951474977505976	5.864818857738555E-4
30-31	0.0	0.0	0.0	0.00427643041710103	5.864818857738555E-4
32-33	0.0	0.0	0.0	0.010849914886816327	5.864818857738555E-4
34-35	0.0	0.0	0.0	0.018767420344763378	6.842288667361648E-4
36-37	0.0	0.0	0.0	0.03130347065317954	6.842288667361648E-4
38-39	0.0	0.0	0.0	0.05114610778852832	6.842288667361648E-4
40-41	0.0	0.0	0.0	0.07265044360023634	6.842288667361648E-4
42-43	0.0	0.0	0.0	0.09566985761686017	6.842288667361648E-4
44-45	0.0	0.0	0.0	0.12218372620288656	6.842288667361648E-4
46-47	0.0	0.0	0.0	0.15170331445350396	6.842288667361648E-4
48-49	0.0	0.0	0.0	0.18283572788999947	6.842288667361648E-4
50-51	0.0	0.0	0.0	0.21756034287685982	6.842288667361648E-4
52-53	0.0	0.0	0.0	0.25318911743762157	8.55286083420206E-4
54-55	0.0	0.0	0.0	0.29067508463666714	8.797228286607833E-4
56-57	0.0	0.0	0.0	0.33346382555291804	8.797228286607833E-4
58-59	0.0	0.0	0.0	0.3844388761247623	8.797228286607833E-4
60-61	0.0	0.0	0.0	0.4397147938589482	9.285963191419379E-4
62-63	0.0	0.0	0.0	0.49662797352425275	9.774698096230924E-4
64-65	0.0	0.0	0.0	0.5530279815395052	9.774698096230924E-4
66-67	0.0	0.0	0.0	0.6167590131269308	9.774698096230924E-4
68-69	0.0	0.0	0.0	0.6914132698368944	9.774698096230924E-4
70-71	0.0	0.0	0.0	0.7760132818597731	9.774698096230924E-4
72-73	0.0	0.0	0.0	0.8639122544901298	9.774698096230924E-4
74-75	0.0	0.0	0.0	0.9494164260869098	9.774698096230924E-4
76-77	0.0	0.0	0.0	1.048776232235097	0.0010019065548636697
78-79	0.0	0.0	0.0	1.1624559710942628	0.001026343300104247
80-81	0.0	0.0	0.0	1.2938767869980876	0.0010507800453448245
82-83	0.0	0.0	0.0	1.4356587828839171	0.0010752167905854018
84-85	0.0	0.0	0.0	1.5780028239102801	0.0010752167905854018
86-87	0.0	0.0	0.0	1.7267981656801552	0.0011240902810665564
88-89	0.0	0.0	0.0	1.8976598884022717	0.0011240902810665564
90-91	0.0	0.0	0.0	2.087973260335888	0.0011240902810665564
92-93	0.0	0.0	0.0	2.290602751870755	0.0011240902810665564
94-95	0.0	0.0	0.0	2.5106800795073942	0.0011240902810665564
96-97	0.0	0.0	0.0	2.740898656418873	0.0011485270263071339
98-99	0.0	0.0	0.0	2.985730406984217	0.001172963771547711
100-101	0.0	0.0	0.0	3.261523513769373	0.001172963771547711
102-103	0.0	0.0	0.0	3.565150073383546	0.0011974005167882883
104-105	0.0	0.0	0.0	3.8846116439136136	0.0012218372620288658
106-107	0.0	0.0	0.0	4.212626075277882	0.001246274007269443
108-109	0.0	0.0	0.0	4.557061999443819	0.0012951474977505976
110-111	0.0	0.0	0.0	4.935367252513197	0.0013195842429911749
112-113	0.0	0.0	0.0	5.339599892282827	0.0013195842429911749
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2890	0.0	78.78551	1
GTATCAA	5670	0.0	58.658638	1
TCAACGC	7525	0.0	43.367214	4
CAACGCA	7675	0.0	42.569115	5
AACGCAG	8070	0.0	40.547344	6
ATCAACG	8200	0.0	40.01522	3
TATCAAC	8400	0.0	38.991585	2
GTACATG	10890	0.0	35.24835	1
ACGCAGA	9505	0.0	34.29892	7
CGCAGAG	10020	0.0	32.593834	8
TACATGG	11725	0.0	32.45454	2
ACATGGG	11645	0.0	31.654732	3
CATGGGG	9025	0.0	29.494959	4
GCAGAGT	10780	0.0	29.357094	9
ATGGGGG	4825	0.0	26.888083	5
CAGAGTA	10310	0.0	26.022795	10-11
AGAGTAC	9830	0.0	25.841068	10-11
GAGTACT	5100	0.0	24.14639	12-13
GTATAGG	780	0.0	22.924902	1
CATGGGT	2245	0.0	22.812326	4
>>END_MODULE