FastQCFastQC Report
Mon 15 Aug 2016
SRR2921500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162697
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.072.588711
GTATCAA46500.063.7355041
TCAACGC60350.047.32154
CAACGCA62000.046.0621345
ATCAACG65550.043.6583063
AACGCAG65650.043.4991656
TATCAAC69550.041.9163482
GTACATG115400.037.027441
ACGCAGA78050.036.2817657
ACATGGG119200.035.1391223
TACATGG120000.035.102372
CGCAGAG84300.033.3778048
CATGGGG98900.031.102034
GCAGAGT90300.030.3036049
ATGGGGG53600.028.4164415
AGAGTAC82150.027.19077310-11
GTATAGG6750.025.5681461
CAGAGTA86300.025.19391810-11
CATGGGT22450.023.3217354
GTATTAC5300.022.4573841
Produced by FastQC (version 0.11.5)