##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921500_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2162697
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.588854102077175	31.0	30.0	34.0	25.0	34.0
2	29.730523045993035	31.0	30.0	34.0	25.0	34.0
3	29.81066418458064	31.0	30.0	34.0	25.0	34.0
4	33.187340159069905	37.0	35.0	37.0	26.0	37.0
5	33.216617491955645	37.0	35.0	37.0	26.0	37.0
6	33.215818027213246	37.0	35.0	37.0	26.0	37.0
7	33.037807885246984	37.0	35.0	37.0	25.0	37.0
8	32.97952463983628	37.0	35.0	37.0	25.0	37.0
9	34.42143305326636	39.0	35.0	39.0	25.0	39.0
10-11	34.30161876582804	39.0	35.0	39.0	25.0	39.0
12-13	34.142488753625685	39.0	35.0	39.0	22.5	39.0
14-15	35.26264566881075	40.0	36.0	41.0	23.0	41.0
16-17	35.17769780047783	40.0	35.0	41.0	22.0	41.0
18-19	35.183627433708935	40.0	35.0	41.0	22.0	41.0
20-21	35.2029905252562	40.0	35.0	41.0	22.5	41.0
22-23	35.16633004993302	40.0	35.0	41.0	23.0	41.0
24-25	35.11885506846313	40.0	35.0	41.0	23.0	41.0
26-27	35.043421940290294	40.0	35.0	41.0	23.0	41.0
28-29	34.98545935930923	40.0	35.0	41.0	23.0	41.0
30-31	34.882173045969914	40.0	34.5	41.0	23.0	41.0
32-33	34.77156231316731	39.5	34.0	41.0	22.5	41.0
34-35	34.60248523024723	39.0	34.0	41.0	21.0	41.0
36-37	34.45096539182326	39.0	34.0	41.0	19.0	41.0
38-39	34.31618391295683	39.0	34.0	41.0	18.0	41.0
40-41	34.15264505383787	39.0	33.0	41.0	16.5	41.0
42-43	33.94499830535669	39.0	33.0	41.0	15.0	41.0
44-45	33.74282990173843	38.5	33.0	41.0	14.5	41.0
46-47	33.48375569948079	38.0	33.0	40.5	9.0	41.0
48-49	33.32783163799645	38.0	33.0	40.0	8.5	41.0
50-51	33.122654028742815	38.0	33.0	40.0	7.5	41.0
52-53	32.92167372498321	38.0	32.0	40.0	6.5	41.0
54-55	32.71436729232065	37.5	32.0	40.0	2.0	41.0
56-57	32.46720067582282	37.0	32.0	40.0	2.0	41.0
58-59	32.220993047107385	36.5	31.5	40.0	2.0	41.0
60-61	31.917412379080382	36.0	31.0	40.0	2.0	41.0
62-63	31.6048847804385	35.5	31.0	39.0	2.0	41.0
64-65	31.29556058939371	35.0	31.0	39.0	2.0	40.5
66-67	31.251949070997924	35.0	31.0	39.0	2.0	40.5
68-69	31.010217566307254	35.0	31.0	38.0	2.0	40.0
70-71	30.71190508887745	35.0	31.0	37.0	2.0	40.0
72-73	30.391918516555947	35.0	30.0	37.0	2.0	39.0
74-75	30.097916860290646	35.0	30.0	36.0	2.0	39.0
76-77	29.823247546928673	35.0	30.0	36.0	2.0	38.5
78-79	29.559926332722522	35.0	30.0	35.0	2.0	37.0
80-81	29.328991532332083	35.0	30.0	35.0	2.0	37.0
82-83	29.13686660683397	35.0	30.0	35.0	2.0	36.0
84-85	28.94624212268293	34.0	29.0	35.0	2.0	36.0
86-87	28.798152723197006	34.0	29.0	35.0	2.0	36.0
88-89	28.680140121339235	34.0	29.0	35.0	2.0	35.5
90-91	28.572226483876385	34.0	29.0	35.0	2.0	35.0
92-93	28.476028310946933	34.0	29.0	35.0	2.0	35.0
94-95	28.412634548436515	34.0	29.0	35.0	2.0	35.0
96-97	28.31198383314907	34.0	29.0	35.0	2.0	35.0
98-99	28.249750889745535	34.0	29.0	35.0	2.0	35.0
100-101	28.18491332812687	34.0	29.0	35.0	2.0	35.0
102-103	27.216992486696007	32.5	26.0	34.5	2.0	35.0
104-105	28.033263790535614	34.0	28.5	35.0	2.0	35.0
106-107	28.36022868668149	34.0	29.0	35.0	2.0	35.0
108-109	28.400619226826507	34.0	29.0	35.0	2.0	35.0
110-111	28.344665017799535	34.0	29.0	35.0	2.0	35.0
112-113	28.27139885984953	34.0	29.0	35.0	2.0	35.0
114-115	28.152877171420684	34.0	29.0	35.0	2.0	35.0
116-117	28.08898125812354	34.0	29.0	35.0	2.0	35.0
118-119	27.933969483473646	34.0	29.0	35.0	2.0	35.0
120-121	27.762944369923297	34.0	29.0	35.0	2.0	35.0
122-123	27.63093419928913	34.0	29.0	35.0	2.0	35.0
124-125	26.78252963776248	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93485.0
3	16856.0
4	10921.0
5	9190.0
6	9710.0
7	11115.0
8	12099.0
9	12548.0
10	13393.0
11	13984.0
12	14079.0
13	14650.0
14	16164.0
15	13799.0
16	9974.0
17	8584.0
18	7953.0
19	7345.0
20	7159.0
21	7381.0
22	8925.0
23	12232.0
24	17379.0
25	23908.0
26	28422.0
27	30227.0
28	35206.0
29	41508.0
30	46537.0
31	56413.0
32	70385.0
33	91714.0
34	127849.0
35	201141.0
36	379601.0
37	537675.0
38	143041.0
39	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.912630407985326	16.978952018187158	12.097559396197441	25.010858177630073
2	16.748322596191425	19.233382405517546	38.34447748299008	25.673817515300946
3	18.98780135283575	24.232548996935886	28.823911660981672	27.955737989246693
4	13.293629594271636	14.897967773551779	35.48863952251906	36.31976310965753
5	14.406767901497245	37.13127379195826	33.35041651826806	15.111541788276433
6	33.85470467009139	34.351990415106386	17.20104453136023	14.592260383442005
7	29.876198511643743	31.115138529628357	20.644374627633393	18.364288331094507
8	27.985019052279753	33.421278792347266	19.358115327579874	19.235586827793107
9	27.874264707922887	14.217188559461183	18.653963385021548	39.254583347594384
10-11	26.580147551978538	24.658178958810336	26.824093989222693	21.937579499988434
12-13	26.733066890601293	22.372216071626394	26.96468751105777	23.93002952671454
14-15	24.200101574403252	23.487855013177843	24.257734409762985	28.05430900265592
16-17	24.441945133258308	26.409794216183784	24.733721597702793	24.414539052855112
18-19	24.553839905110316	25.57980688986468	26.18124059753857	23.68511260748644
20-21	25.717215650831406	24.600964684747122	25.75161415015928	23.93020551426219
22-23	25.45977395685607	24.19653045216725	25.622872359250426	24.72082323172626
24-25	24.873048300520946	24.686845534522696	25.730219512911702	24.70988665204466
26-27	24.583426624798076	24.715892794691545	25.821674213252262	24.879006367258118
28-29	24.62531353016227	24.73904483284714	25.83629631480047	24.799345322190117
30-31	24.935669560466682	24.676173578039965	25.724519768209188	24.66363709328417
32-33	24.66017539975929	24.738199215875685	25.635646590280587	24.96597879408444
34-35	24.88264083504541	24.789502862483527	25.78376864407348	24.54408765839758
36-37	24.974666067426543	24.607049367686788	25.65905812128043	24.759226443606238
38-39	24.969187122993347	24.756990035319145	25.54253333666445	24.731289505023053
40-41	24.997877793765962	24.688232291258753	25.673791805438906	24.640098109536375
42-43	24.93638076467996	24.709199777468577	25.521323807508008	24.833095650343452
44-45	24.80172733457461	24.789027705778516	25.50846514692129	24.90077981272558
46-47	24.860441880644988	24.842929884233442	25.428228459145576	24.868399775975995
48-49	25.05406686944152	24.956480507014238	25.122069691327326	24.867382932216913
50-51	24.975712637478036	25.143387962165377	25.07089596774268	24.810003432613904
52-53	25.10800679917721	24.920377082294287	25.20347564908177	24.768140469446728
54-55	24.98645161961838	24.984069232628524	25.31108075944946	24.718398388303637
56-57	24.91379772927445	25.031154939272156	25.116755852595276	24.938291478858122
58-59	25.001434040608327	24.87102886399929	25.12344314075247	25.004093954639906
60-61	24.91504272262923	25.057806101752618	25.346223743263664	24.680927432354487
62-63	24.77840184735285	25.128758680282502	25.26406284404264	24.828776628322004
64-65	24.853063486380446	24.825147167800434	25.3537300549815	24.96805929083762
66-67	24.76853634580102	25.030059689210916	25.267230001084666	24.934173963903394
68-69	24.883697588754302	25.10332852921968	25.202748705386956	24.810225176639054
70-71	24.769197496810875	24.83012338996851	25.37945104243648	25.021228070784137
72-73	24.958846375682754	25.147489316708764	25.185624931487716	24.70803937612077
74-75	24.799046199426193	25.2684298717203	25.173304222834087	24.75921970601942
76-77	24.865559661029728	25.001104292123312	25.13396624496969	24.999369801877275
78-79	24.84638619905259	25.060863882064567	25.208828968964564	24.88392094991828
80-81	24.818573218442424	25.249252254798083	25.023668384541992	24.908506142217508
82-83	24.976414112685045	25.16796851515278	25.03297399540306	24.822643376759114
84-85	24.778413596043666	25.22305474411456	25.001370065423224	24.99716159441855
86-87	24.603715244730925	25.299676183235338	25.11825185193407	24.97835672009967
88-89	24.738531168139236	25.11032703606684	25.071940349864587	25.079201445929343
90-91	24.72930890646995	25.08643062335486	25.135333985686458	25.04892648448873
92-93	24.77479362778752	25.249705810193756	25.002399071911807	24.973101490106924
94-95	24.738611102247464	25.065841469003523	25.143972574802614	25.051574853946395
96-97	24.731306112530156	25.085025028306994	25.12017032520729	25.06349853395556
98-99	24.684710639466758	25.072833784517435	25.04053257912986	25.20192299688595
100-101	24.652727760807654	25.19461049115117	25.092388970804326	25.060272777236857
102-103	24.699766805128533	25.003664622955384	25.28980578191956	25.00676278999653
104-105	24.600886951282416	25.164080900721892	25.10651068297468	25.12852146502101
106-107	24.61237173469718	25.163837392659456	25.107005285524693	25.116785587118674
108-109	24.66159488684684	25.12265176609757	25.085008807250013	25.130744539805576
110-111	24.658536038363728	25.260483680594813	25.102754208142326	24.978226072899133
112-113	24.673402317570726	25.259130191910096	25.110183799201213	24.957283691317965
114-115	24.407605128196828	25.487274189375377	25.131623394895154	24.973497287532638
116-117	24.510796759561757	25.531976877265556	25.012774288836265	24.944452074336418
118-119	24.685290895034548	25.179092191691684	25.057171381586873	25.078445531686892
120-121	24.31488662124417	25.324306371933176	25.289412391400024	25.071394615422626
122-123	24.43985424312131	25.355702702033934	25.144171609599674	25.060271445245082
124-125	24.363699166334122	25.55496419384477	25.092492105629425	24.988844534191674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	153.0
2	187.0
3	206.5
4	188.0
5	176.5
6	200.0
7	253.0
8	322.0
9	398.5
10	456.0
11	501.5
12	579.5
13	664.0
14	782.0
15	877.5
16	951.5
17	1103.0
18	1234.0
19	1336.0
20	1503.5
21	1642.5
22	1898.5
23	2350.5
24	2860.5
25	3572.5
26	4649.0
27	6040.5
28	7342.0
29	8882.5
30	11095.5
31	13186.0
32	15800.5
33	18655.0
34	21507.0
35	24826.5
36	28939.0
37	32853.0
38	35940.0
39	40103.5
40	44146.0
41	47401.0
42	51131.0
43	57251.0
44	63323.0
45	67940.0
46	73256.0
47	78396.5
48	84463.5
49	90428.0
50	91652.0
51	91381.5
52	91229.0
53	91535.5
54	92456.0
55	91402.5
56	86988.0
57	79548.5
58	74749.0
59	69927.5
60	62391.0
61	55694.5
62	48643.5
63	38818.0
64	29761.0
65	23811.0
66	18598.5
67	14288.0
68	11539.0
69	9403.0
70	7942.5
71	6495.5
72	5237.5
73	4693.0
74	3842.0
75	3005.0
76	2144.5
77	1301.5
78	786.5
79	493.5
80	301.5
81	145.5
82	83.0
83	46.5
84	43.5
85	34.5
86	29.5
87	24.0
88	18.0
89	16.5
90	15.5
91	12.5
92	8.5
93	10.5
94	10.5
95	11.0
96	10.5
97	12.0
98	13.0
99	11.0
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033846627613576936
2	0.026633411892650706
3	0.026448457643396185
4	0.031627176622522714
5	0.033615434802008785
6	0.04448149694571176
7	0.03925653940427161
8	0.034725160297535904
9	0.02917653281990034
10-11	0.03454020604828138
12-13	0.03497947239026086
14-15	0.03398534330051783
16-17	0.03551121585686761
18-19	0.04757948062072495
20-21	0.05851490060789838
22-23	0.05171783194779481
24-25	0.0621215084683615
26-27	0.04683966362370688
28-29	0.04730204924684318
30-31	0.04653911296866829
32-33	0.05444590712429897
34-35	0.039325897247742055
36-37	0.04949838095674059
38-39	0.058399304202114304
40-41	0.047695077026509027
42-43	0.05569434830676697
44-45	0.056249211054530526
46-47	0.06110426009746164
48-49	0.04725581068452955
50-51	0.049891408736406434
52-53	0.05821434995285979
54-55	0.04621544303247288
56-57	0.04258571589085295
58-59	0.044990121131161694
60-61	0.033661673364322416
62-63	0.04166094464458035
64-65	0.0406436962736805
66-67	0.03460956389175183
68-69	0.030563689689309226
70-71	0.048758563959722515
72-73	0.03125726812401367
74-75	0.03789250181601954
76-77	0.03137286452979775
78-79	0.032390112900697605
80-81	0.024506438026223737
82-83	0.017894323615374692
84-85	0.018102397145786025
86-87	0.016784598119847578
88-89	0.02261065697136492
90-91	0.012438173262366388
92-93	0.018611021331235954
94-95	0.014149000067970686
96-97	0.01135156704799609
98-99	0.011051016392957498
100-101	0.010935419987173423
102-103	0.005918535976144601
104-105	0.005571746758792378
106-107	0.008646611152648753
108-109	0.012669366073934535
110-111	0.006172848068869564
112-113	0.008045509842571567
114-115	0.008438537622237419
116-117	0.0069357843470444535
118-119	0.23808235735287928
120-121	0.3512974771778016
122-123	0.02790497235627552
124-125	0.0036990849850903756
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2162697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.07710618338988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59451619532516	45.4302465249612
2	10.563131544255297	12.058259615611494
3	3.6147517818583546	6.189587138391813
4	1.7338594270766523	3.9585471450530254
5	1.0321799052515974	2.9456921026203364
6	0.6832844140088161	2.3399938231101918
7	0.49561719733081205	1.9801876778855396
8	0.360508777226645	1.6461438206247414
9	0.2710115962777322	1.3921701891906701
>10	1.542791655649301	15.715060019625996
>50	0.08034360852189487	3.1123173148701824
>100	0.026861894889362567	2.65414076487747
>500	8.972875436870484E-4	0.3470951519291609
>1k	2.447147846419189E-4	0.23055871124791225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6238562313629694E-5
2	0.0	0.0	0.0	0.0	4.6238562313629694E-5
3	0.0	0.0	0.0	0.0	4.6238562313629694E-5
4	0.0	0.0	0.0	0.0	4.6238562313629694E-5
5	0.0	0.0	0.0	0.0	4.6238562313629694E-5
6	0.0	0.0	0.0	0.0	1.3871568694088908E-4
7	0.0	0.0	0.0	0.0	1.3871568694088908E-4
8	0.0	0.0	0.0	0.0	1.3871568694088908E-4
9	0.0	0.0	0.0	0.0	1.8495424925451878E-4
10-11	0.0	0.0	0.0	0.0	2.0807353041133364E-4
12-13	0.0	0.0	0.0	4.6238562313629694E-5	2.3119281156814848E-4
14-15	0.0	0.0	0.0	4.6238562313629694E-5	3.0055065503859304E-4
16-17	0.0	0.0	0.0	4.6238562313629694E-5	3.6990849850903755E-4
18-19	0.0	0.0	0.0	4.6238562313629694E-5	4.1614706082266723E-4
20-21	0.0	0.0	0.0	9.247712462725939E-5	4.1614706082266723E-4
22-23	0.0	0.0	0.0	1.3871568694088908E-4	4.6238562313629696E-4
24-25	0.0	0.0	0.0	1.8495424925451878E-4	5.086241854499266E-4
26-27	0.0	0.0	0.0	1.8495424925451878E-4	5.086241854499266E-4
28-29	0.0	0.0	0.0	6.473398723908157E-4	5.086241854499266E-4
30-31	0.0	0.0	0.0	0.00376844282856082	5.317434666067414E-4
32-33	0.0	0.0	0.0	0.008692849714962383	6.011013100771861E-4
34-35	0.0	0.0	0.0	0.014935055627302391	6.011013100771861E-4
36-37	0.0	0.0	0.0	0.02385909815383292	6.011013100771861E-4
38-39	0.0	0.0	0.0	0.0400194756824465	6.473398723908158E-4
40-41	0.0	0.0	0.0	0.058445542764427934	6.935784347044454E-4
42-43	0.0	0.0	0.0	0.07636298566095945	7.398169970180751E-4
44-45	0.0	0.0	0.0	0.09696226517168147	7.629362781748899E-4
46-47	0.0	0.0	0.0	0.11980411495461454	7.860555593317048E-4
48-49	0.0	0.0	0.0	0.14350137814034974	8.322941216453345E-4
50-51	0.0	0.0	0.0	0.17401882926734535	8.554134028021493E-4
52-53	0.0	0.0	0.0	0.20402765620889102	0.0010172483708998533
54-55	0.0	0.0	0.0	0.23563171355025692	0.001063486933213483
56-57	0.0	0.0	0.0	0.27181338856067216	0.001086606214370298
58-59	0.0	0.0	0.0	0.3129657090198026	0.0011097254955271126
60-61	0.0	0.0	0.0	0.3584182157741006	0.0011097254955271126
62-63	0.0	0.0	0.0	0.4065525591425891	0.0012484411824680017
64-65	0.0	0.0	0.0	0.45521864597768436	0.0012946797447816315
66-67	0.0	0.0	0.0	0.5106818014728831	0.0013177990259384464
68-69	0.0	0.0	0.0	0.5745141367468489	0.001340918307095261
70-71	0.0	0.0	0.0	0.6416062906639257	0.0013871568694088909
72-73	0.0	0.0	0.0	0.7158423024584581	0.001502753275192965
74-75	0.0	0.0	0.0	0.7938698763627082	0.0015489918375065949
76-77	0.0	0.0	0.0	0.8782552525850824	0.0015721111186634096
78-79	0.0	0.0	0.0	0.9737378837627277	0.0015721111186634096
80-81	2.3119281156814847E-5	0.0	0.0	1.0801328156463896	0.0015952303998202245
82-83	4.6238562313629694E-5	0.0	0.0	1.1964921577086387	0.001641468962133854
84-85	4.6238562313629694E-5	0.0	0.0	1.3148859965126876	0.001664588243290669
86-87	4.6238562313629694E-5	0.0	0.0	1.4405161703188196	0.001664588243290669
88-89	4.6238562313629694E-5	0.0	0.0	1.5840637870214829	0.0016877075244474838
90-91	4.6238562313629694E-5	0.0	0.0	1.745598204464148	0.0017108268056042987
92-93	4.6238562313629694E-5	0.0	0.0	1.9267377723277925	0.0017108268056042987
94-95	4.6238562313629694E-5	4.6238562313629694E-5	0.0	2.1110215624287636	0.0017801846490747434
96-97	4.6238562313629694E-5	4.6238562313629694E-5	0.0	2.303928844401227	0.0018726617737020025
98-99	4.6238562313629694E-5	4.6238562313629694E-5	0.0	2.511100722847445	0.0019189003360156323
100-101	4.6238562313629694E-5	4.6238562313629694E-5	0.0	2.749946016478499	0.0019420196171724472
102-103	4.6238562313629694E-5	4.6238562313629694E-5	0.0	3.014638666442872	0.0020344967417997065
104-105	4.6238562313629694E-5	4.6238562313629694E-5	0.0	3.2905904063306144	0.0020807353041133363
106-107	4.6238562313629694E-5	4.6238562313629694E-5	0.0	3.5788647230749384	0.002126973866426966
108-109	4.6238562313629694E-5	4.6238562313629694E-5	0.0	3.8679019760974374	0.0021963317098974105
110-111	4.6238562313629694E-5	4.6238562313629694E-5	0.0	4.19226549072755	0.0023119281156814848
112-113	4.6238562313629694E-5	4.6238562313629694E-5	0.0	4.548487374791753	0.0023119281156814848
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2230	0.0	72.58871	1
GTATCAA	4650	0.0	63.735504	1
TCAACGC	6035	0.0	47.3215	4
CAACGCA	6200	0.0	46.062134	5
ATCAACG	6555	0.0	43.658306	3
AACGCAG	6565	0.0	43.499165	6
TATCAAC	6955	0.0	41.916348	2
GTACATG	11540	0.0	37.02744	1
ACGCAGA	7805	0.0	36.281765	7
ACATGGG	11920	0.0	35.139122	3
TACATGG	12000	0.0	35.10237	2
CGCAGAG	8430	0.0	33.377804	8
CATGGGG	9890	0.0	31.10203	4
GCAGAGT	9030	0.0	30.303604	9
ATGGGGG	5360	0.0	28.416441	5
AGAGTAC	8215	0.0	27.190773	10-11
GTATAGG	675	0.0	25.568146	1
CAGAGTA	8630	0.0	25.193918	10-11
CATGGGT	2245	0.0	23.321735	4
GTATTAC	530	0.0	22.457384	1
>>END_MODULE