FastQCFastQC Report
Mon 15 Aug 2016
SRR2921500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162697
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32830.1518012000756463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30900.14287715754911576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.080.579191
GTATCAA61350.061.2281461
TCAACGC83050.044.8076364
CAACGCA84300.044.0522165
ATCAACG87850.042.427193
AACGCAG88250.042.2036366
TATCAAC92250.040.275412
ACGCAGA104950.035.203787
GTACATG108100.033.976621
CGCAGAG109850.033.5243768
TACATGG111650.032.9039572
ACATGGG114650.031.9903013
GCAGAGT117500.030.9366659
CATGGGG93950.029.8494134
AGAGTAC108750.028.39312710-11
GTGGTAT10500.027.2569371
ATGGGGG52550.026.160745
CAGAGTA115400.025.88052410-11
GAGTACT62950.024.80925812-13
AGTACTT66750.024.37733712-13
Produced by FastQC (version 0.11.5)