##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921500_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2162697
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25402263932488	31.0	31.0	34.0	28.0	34.0
2	31.32251582168006	33.0	31.0	34.0	28.0	34.0
3	31.407799613168187	33.0	31.0	34.0	28.0	34.0
4	34.91803844921411	37.0	35.0	37.0	32.0	37.0
5	34.57750715888541	37.0	35.0	37.0	31.0	37.0
6	34.55340623305068	37.0	35.0	37.0	31.0	37.0
7	34.395701293338824	37.0	35.0	37.0	30.0	37.0
8	34.3680131798398	37.0	35.0	37.0	30.0	37.0
9	35.94686171941793	39.0	37.0	39.0	30.0	39.0
10-11	35.81734103297873	39.0	37.0	39.0	30.0	39.0
12-13	35.71699780413067	39.0	36.0	39.0	30.0	39.0
14-15	36.90210464064083	40.0	37.0	41.0	30.0	41.0
16-17	36.88182856868068	40.0	37.0	41.0	30.0	41.0
18-19	36.89476866153696	40.0	37.0	41.0	30.0	41.0
20-21	36.875018322030314	40.0	37.0	41.0	30.0	41.0
22-23	36.80931401856108	40.0	37.0	41.0	30.0	41.0
24-25	36.74268979889462	40.0	37.0	41.0	30.0	41.0
26-27	36.61454193537051	40.0	36.0	41.0	30.0	41.0
28-29	36.427486605844464	40.0	36.0	41.0	29.0	41.0
30-31	36.372905913311016	40.0	36.0	41.0	29.0	41.0
32-33	36.24630195538256	40.0	35.5	41.0	29.0	41.0
34-35	36.11376142843866	40.0	35.0	41.0	28.5	41.0
36-37	35.97803159665917	40.0	35.0	41.0	28.0	41.0
38-39	35.802378465406846	40.0	35.0	41.0	27.0	41.0
40-41	35.59568862397275	39.5	35.0	41.0	26.5	41.0
42-43	35.42112325489886	39.0	35.0	41.0	26.0	41.0
44-45	35.19831927449846	39.0	35.0	41.0	25.0	41.0
46-47	34.91294480918964	39.0	34.5	41.0	24.0	41.0
48-49	34.65059853506987	39.0	34.0	41.0	23.0	41.0
50-51	34.42219645193016	39.0	34.0	41.0	22.5	41.0
52-53	34.21784697532756	38.0	34.0	40.5	22.0	41.0
54-55	33.99315206892135	38.0	33.5	40.0	21.5	41.0
56-57	33.7331283577866	37.5	33.0	40.0	20.5	41.0
58-59	33.58189057459274	37.0	33.0	40.0	20.5	41.0
60-61	33.456452522013024	37.0	33.0	40.0	20.0	41.0
62-63	33.19254130375175	36.0	33.0	40.0	20.0	41.0
64-65	32.89381730311736	36.0	33.0	39.0	19.5	41.0
66-67	32.59341946652721	35.0	33.0	39.0	18.5	41.0
68-69	32.2773419022637	35.0	32.0	38.5	18.0	40.5
70-71	31.974926908392625	35.0	32.0	37.5	17.0	40.0
72-73	31.668729137738666	35.0	32.0	37.0	15.5	39.0
74-75	31.38333409626961	35.0	32.0	36.5	13.0	39.0
76-77	31.096264987651992	35.0	32.0	36.0	12.0	39.0
78-79	30.826918657583562	35.0	31.0	35.5	10.0	37.0
80-81	30.610680784224513	35.0	31.0	35.0	8.0	37.0
82-83	30.431699170064043	35.0	31.0	35.0	7.0	36.5
84-85	30.2433708929175	35.0	31.0	35.0	7.0	36.0
86-87	30.102523839446764	35.0	31.0	35.0	7.0	36.0
88-89	30.00492856835701	35.0	31.0	35.0	5.5	35.5
90-91	29.910016752231126	35.0	31.0	35.0	2.0	35.0
92-93	29.97295136581777	35.0	31.0	35.0	2.0	35.0
94-95	29.944630477593485	35.0	31.0	35.0	2.0	35.0
96-97	29.926691302572667	35.0	31.0	35.0	2.0	35.0
98-99	29.887068322562058	35.0	31.0	35.0	2.0	35.0
100-101	29.836159896647565	35.0	31.0	35.0	2.0	35.0
102-103	28.950860430286816	33.5	29.5	35.0	2.0	35.0
104-105	29.659383630716647	34.5	31.0	35.0	2.0	35.0
106-107	29.76531895129091	35.0	31.0	35.0	2.0	35.0
108-109	29.7704149032435	35.0	31.0	35.0	2.0	35.0
110-111	29.7388522294154	35.0	31.0	35.0	2.0	35.0
112-113	29.69330331525868	35.0	31.0	35.0	2.0	35.0
114-115	29.631619223589805	35.0	31.0	35.0	2.0	35.0
116-117	29.555135324088397	35.0	31.0	35.0	2.0	35.0
118-119	29.465449852660818	35.0	31.0	35.0	2.0	35.0
120-121	29.341362659679096	35.0	31.0	35.0	2.0	35.0
122-123	29.221966368844086	35.0	31.0	35.0	2.0	35.0
124-125	28.3047976669871	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35135.0
3	13112.0
4	7493.0
5	6477.0
6	6792.0
7	7952.0
8	9117.0
9	10275.0
10	11807.0
11	12847.0
12	13466.0
13	15104.0
14	19372.0
15	15907.0
16	9197.0
17	7654.0
18	6892.0
19	6469.0
20	6099.0
21	6418.0
22	7576.0
23	10119.0
24	14420.0
25	20200.0
26	25082.0
27	27698.0
28	32310.0
29	38628.0
30	44931.0
31	53831.0
32	67219.0
33	87057.0
34	120655.0
35	194095.0
36	389810.0
37	614453.0
38	186658.0
39	370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07485888587313	17.480236152344464	12.399572062990623	25.045332898791788
2	17.122284660560318	19.65170782186994	37.27758365018748	25.948423867382264
3	19.090554664256846	24.29722933796675	29.097662957743808	27.514553040032595
4	13.443190871380814	15.381921344124775	35.06504381748313	36.10984396701128
5	14.378973725944896	37.14104943596043	33.281641461290526	15.198335376804147
6	33.99218041106952	34.77834785978757	16.516176115567863	14.713295613575042
7	29.53845570440965	31.406081565096834	20.548601511951464	18.506861218542053
8	28.209261450574942	33.269058165327756	19.064754688503577	19.45692569559373
9	27.984082182254017	13.833297574040232	18.576644495904944	39.605975747800805
10-11	26.68895844730087	24.543070134023512	26.693189600519023	22.074781818156595
12-13	26.968094231584672	22.278132625415765	27.091141304372957	23.66263183862661
14-15	24.333992870707707	23.21967680973187	24.59191991961628	27.85441039994414
16-17	24.282314074963317	26.204166437376504	25.04694860647842	24.46657088118176
18-19	24.381384758519022	25.009433211934677	27.05815112745379	23.55103090209251
20-21	25.437976043952638	24.033715027872727	26.867787155908996	23.660521772265636
22-23	25.18545099568855	23.64988923691739	26.767371168118732	24.39728859927533
24-25	24.64160662710094	24.16569879626442	26.7874464106556	24.40524816597904
26-27	24.38738983282844	24.27277759306435	26.851032758707593	24.488799815399613
28-29	24.46459039584274	24.330826149719304	26.840900088860153	24.363683365577803
30-31	24.725053393752212	24.255539861939344	26.714820477671747	24.3045862666367
32-33	24.382581383137016	24.337932136382058	26.640825134728118	24.63866134575281
34-35	24.635918373198727	24.338821198512388	26.772992368385694	24.25226805990319
36-37	24.797335902720306	24.228010433856902	26.498375519421046	24.476278144001746
38-39	24.76746358640707	24.396783523768384	26.415290240407874	24.42046264941667
40-41	24.799495023647644	24.352970146510195	26.464090377818962	24.383444452023202
42-43	24.778490766103683	24.401819287864086	26.27026334633452	24.54942659969771
44-45	24.73553393945462	24.435741377871125	26.08685498063777	24.741869702036485
46-47	24.835942074025155	24.58956614713834	25.851667586963416	24.722824191873087
48-49	25.09933497652566	24.79030567400831	25.337396684802833	24.772962664663204
50-51	24.944337295295476	25.096324400092584	25.242045187317807	24.717293117294133
52-53	25.159568244242898	24.87548797751962	25.336676161445464	24.628267616792023
54-55	24.991409685619928	24.87149213413932	25.452581233388784	24.684516946851968
56-57	24.84896484903422	24.951242805510013	25.28527063306887	24.914521712386893
58-59	24.92134681810131	24.674114767282312	25.521182829165244	24.883355585451138
60-61	24.880727529695562	24.905030625137588	25.627671953071673	24.58656989209518
62-63	24.805161500379583	24.994710345454333	25.484434078648547	24.715694075517536
64-65	24.867168687096182	24.647613753503745	25.62685881750649	24.85835874189358
66-67	24.831873831325147	24.85573578161876	25.531939983495484	24.78045040356061
68-69	24.900523565052282	24.888753121537807	25.378648692960464	24.832074620449447
70-71	24.731711885236898	24.7423252522987	25.62446022704281	24.901502635421593
72-73	25.01048645593862	25.0285226976853	25.27723784669472	24.68375299968136
74-75	24.837419241459365	25.137908070535676	25.201243115002015	24.823429573002944
76-77	24.90619803954076	24.834264127175274	25.227923676523535	25.031614156760433
78-79	24.864436098571748	24.973682749944498	25.238474062014355	24.9234070894694
80-81	24.837511767346125	25.11195021259267	25.11312954868195	24.937408471379246
82-83	24.975485441438934	25.016664813093975	25.127324258765533	24.88052548670156
84-85	24.806946302897707	25.0628868212946	25.019416934711696	25.11074994109599
86-87	24.64173860566101	25.144138113620258	25.163560775010435	25.050562505708296
88-89	24.720736953368327	24.943490668416075	25.15130626450599	25.184466113709608
90-91	24.65702334204345	24.9926413751946	25.18806980207669	25.16226548068526
92-93	24.746136880654106	25.068097210979623	25.127865071223177	25.057900837143098
94-95	24.640744868279285	24.88037160178125	25.24543012120063	25.23345340873884
96-97	24.67162971354588	24.90964788569579	25.226111483596515	25.19261091716182
98-99	24.594370583414154	24.889146802024822	25.137059381802253	25.379423232758768
100-101	24.607033375399602	24.986022869314176	25.196824358913293	25.210119396372928
102-103	24.706271585437527	24.810566038433812	25.310814116350162	25.1723482597785
104-105	24.560184237911237	24.961638295073357	25.13025177347023	25.347925693545175
106-107	24.605346539735148	24.958425589260774	25.077721548896196	25.35850632210788
108-109	24.66929059024869	24.847319974462184	25.073354164705968	25.41003527058316
110-111	24.650942349307343	24.98712245917103	25.09291712134944	25.269018070172194
112-113	24.588681129898564	24.89092460329388	25.190485989562983	25.329908277244574
114-115	24.306398306701176	25.144793926874183	25.20402701787281	25.34478074855183
116-117	24.387586672187975	25.14399218878069	25.07684862710607	25.391572511925265
118-119	24.422505198875182	24.79896988976119	25.18613984731469	25.59238506404894
120-121	24.164924360845617	24.970735504682203	25.323689710110987	25.540650424361193
122-123	24.235943093819827	24.991393789888253	25.229061593645852	25.543601522646064
124-125	24.25248992732504	25.08392643195247	25.09463331023145	25.568950330491035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	48.0
2	86.0
3	123.5
4	94.5
5	84.0
6	157.0
7	321.0
8	547.0
9	659.5
10	574.0
11	491.0
12	560.5
13	725.0
14	881.5
15	1039.0
16	1234.0
17	1432.5
18	1652.0
19	1893.0
20	2144.5
21	2397.5
22	2730.0
23	3188.5
24	3653.5
25	4360.0
26	5449.5
27	6718.5
28	7821.0
29	9332.0
30	11526.5
31	13496.0
32	15933.0
33	18759.5
34	21568.0
35	24538.5
36	28371.0
37	32353.5
38	35483.0
39	39322.0
40	43059.0
41	46472.5
42	50106.0
43	55816.5
44	62446.5
45	67531.0
46	73129.0
47	78472.5
48	84629.5
49	90629.0
50	91636.5
51	91510.0
52	91522.5
53	91924.0
54	93339.5
55	91905.5
56	87016.5
57	79879.0
58	74915.5
59	70286.5
60	62925.5
61	55690.5
62	48372.0
63	38370.0
64	29142.5
65	23191.5
66	17944.5
67	13736.5
68	11051.5
69	9040.5
70	7664.0
71	6236.5
72	5030.5
73	4742.5
74	3899.0
75	2910.5
76	2060.5
77	1203.0
78	696.5
79	399.5
80	208.5
81	82.5
82	44.0
83	26.0
84	12.0
85	4.0
86	4.0
87	2.5
88	1.5
89	1.5
90	0.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1186481508967738
2	0.0013871568694088909
3	0.020067536044115288
4	0.05691967020807815
5	0.02432148377696922
6	0.02011377460642892
7	0.015073771314243279
8	0.0055486274776355635
9	0.004115232045913043
10-11	0.007675601344062529
12-13	0.0050862418544992665
14-15	0.0032366993619540787
16-17	0.01044991508288031
18-19	0.005779820289203712
20-21	0.01287743960434587
22-23	0.009178354619255494
24-25	0.014033403662186611
26-27	0.008692849714962383
28-29	0.01435707359838202
30-31	0.02122350010195603
32-33	0.013247348102854908
34-35	0.02041432526146751
36-37	0.01449578928532291
38-39	0.02103854585270151
40-41	0.010149364427841718
42-43	0.009363308868510013
44-45	0.017038910212572544
46-47	0.016229735372084025
48-49	0.02046056382378114
50-51	0.015836707592418172
52-53	0.02108478441501514
54-55	0.017478176554552024
56-57	0.02089983016576062
58-59	0.016460928183652173
60-61	0.0194664347340381
62-63	0.021154142258485584
64-65	0.01701579093141573
66-67	0.011952668358073276
68-69	0.023188639000285293
70-71	0.015235606282340983
72-73	0.017755607928433805
74-75	0.017894323615374692
76-77	0.013663495163677573
78-79	0.02852919294750952
80-81	0.021431573632367362
82-83	0.009548263117764532
84-85	0.01296991672897313
86-87	0.01269248535509135
88-89	0.020506802386094772
90-91	0.012415053981209572
92-93	0.0075600049382784544
94-95	0.0077218399063761585
96-97	0.002173212428740596
98-99	0.001826423211388373
100-101	0.010912300706016608
102-103	0.0032366993619540787
104-105	0.0027049558953473373
106-107	3.930277796658524E-4
108-109	0.01911964551668588
110-111	7.860555593317048E-4
112-113	0.009340189587353198
114-115	0.0025200016460928186
116-117	0.007698720625219344
118-119	0.009710098085862236
120-121	0.004323305576324377
122-123	6.704591535476305E-4
124-125	0.0247607501189487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2162697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.13013383484885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65634236161998	43.363346813558
2	10.896385887180587	12.014384245528479
3	3.811467852052066	6.303801983725628
4	1.8282318678219216	4.031626702166329
5	1.080655861006938	2.978835112336315
6	0.7371374844009785	2.4383092901825845
7	0.5174974737239175	1.9970793489917025
8	0.3879280908220788	1.7109222052254882
9	0.301804505816017	1.497467051783769
>10	1.6614686172221769	16.460227483517144
>50	0.0884061472419158	3.3468661188876196
>100	0.030738664212256458	2.9065978649172077
>500	0.001598632639535783	0.5079698085983441
>1k	3.365542399022152E-4	0.4425659705813258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3283	0.1518012000756463	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3090	0.14287715754911576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.6238562313629694E-5	0.0
4	0.0	0.0	0.0	4.6238562313629694E-5	9.247712462725939E-5
5	0.0	0.0	0.0	4.6238562313629694E-5	9.247712462725939E-5
6	0.0	0.0	0.0	4.6238562313629694E-5	9.247712462725939E-5
7	0.0	0.0	0.0	4.6238562313629694E-5	9.247712462725939E-5
8	0.0	0.0	0.0	4.6238562313629694E-5	1.3871568694088908E-4
9	0.0	0.0	0.0	4.6238562313629694E-5	1.8495424925451878E-4
10-11	0.0	0.0	0.0	4.6238562313629694E-5	1.8495424925451878E-4
12-13	0.0	0.0	0.0	4.6238562313629694E-5	2.7743137388177815E-4
14-15	0.0	0.0	0.0	4.6238562313629694E-5	3.6990849850903755E-4
16-17	0.0	0.0	0.0	4.6238562313629694E-5	3.6990849850903755E-4
18-19	0.0	0.0	0.0	9.247712462725939E-5	3.6990849850903755E-4
20-21	0.0	0.0	0.0	9.247712462725939E-5	4.8550490429311174E-4
22-23	0.0	0.0	0.0	1.1559640578407424E-4	6.24220591234001E-4
24-25	0.0	0.0	0.0	1.8495424925451878E-4	6.473398723908158E-4
26-27	0.0	0.0	0.0	1.8495424925451878E-4	6.935784347044454E-4
28-29	0.0	0.0	0.0	6.473398723908157E-4	6.935784347044454E-4
30-31	0.0	0.0	0.0	0.003745323547404005	6.935784347044454E-4
32-33	0.0	0.0	0.0	0.008808446120746457	7.398169970180751E-4
34-35	0.0	0.0	0.0	0.01512000987655691	7.398169970180751E-4
36-37	0.0	0.0	0.0	0.024182768090028332	7.629362781748899E-4
38-39	0.0	0.0	0.0	0.04087488908524865	8.322941216453345E-4
40-41	0.0	0.0	0.0	0.05971710322805275	8.554134028021493E-4
42-43	0.0	0.0	0.0	0.07788885821730922	9.01651965115779E-4
44-45	0.0	0.0	0.0	0.09858061485265851	9.247712462725939E-4
46-47	0.0	0.0	0.0	0.12167677672831655	9.247712462725939E-4
48-49	0.0	0.0	0.0	0.14588266409950168	9.247712462725939E-4
50-51	0.0	0.0	0.0	0.17725552862929944	9.710098085862236E-4
52-53	0.0	0.0	0.0	0.20793481472439274	0.0010403676520566681
54-55	0.0	0.0	0.0	0.24025556978161988	0.0011097254955271126
56-57	0.0	0.0	0.0	0.2770383461021123	0.0011328447766839275
58-59	0.0	0.0	0.0	0.31913855708867217	0.0011790833389975573
60-61	0.0	0.0	0.0	0.36583950502543816	0.0012022026201543722
62-63	0.0	0.0	0.0	0.41517605101408106	0.0012022026201543722
64-65	0.0	0.0	0.0	0.46546048753015334	0.0012022026201543722
66-67	0.0	0.0	0.0	0.5217096985846839	0.0012022026201543722
68-69	0.0	0.0	0.0	0.5866517593541768	0.0012253219013111868
70-71	0.0	0.0	0.0	0.6548074002044669	0.0012484411824680017
72-73	0.0	0.0	0.0	0.730592403836506	0.0014333954317225206
74-75	0.0	0.0	0.0	0.8100071346101649	0.0014796339940361502
76-77	0.0	0.0	0.0	0.8957334291396345	0.0014796339940361502
78-79	0.0	0.0	0.0	0.9931349606532953	0.0014796339940361502
80-81	0.0	0.0	0.0	1.1025353990873432	0.001502753275192965
82-83	0.0	0.0	0.0	1.2213916235145286	0.0017108268056042987
84-85	0.0	0.0	0.0	1.342189867558886	0.0017108268056042987
86-87	0.0	0.0	0.0	1.4711260985704424	0.001803303930231558
88-89	0.0	0.0	0.0	1.6175405061365509	0.0018957810548588174
90-91	0.0	0.0	0.0	1.7833751098743837	0.0018957810548588174
92-93	0.0	0.0	0.0	1.9684680748158434	0.0019882581794860767
94-95	0.0	0.0	0.0	2.1568902162438848	0.0019882581794860767
96-97	0.0	0.0	0.0	2.354560070134651	0.0022425702722110403
98-99	0.0	0.0	0.0	2.566610116904957	0.0023119281156814848
100-101	0.0	0.0	0.0	2.8109577994513333	0.0023119281156814848
102-103	0.0	0.0	0.0	3.08092164551946	0.0023812859591519292
104-105	0.0	0.0	0.0	3.362283297197897	0.0024968823649360035
106-107	0.0	0.0	0.0	3.6554589015474663	0.002520001646092818
108-109	0.0	0.0	0.0	3.950807718325776	0.0025893594895632626
110-111	0.0	0.0	0.0	4.284395826137457	0.0027049558953473373
112-113	0.0	0.0	0.0	4.650535881817934	0.002728075176504152
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2945	0.0	80.57919	1
GTATCAA	6135	0.0	61.228146	1
TCAACGC	8305	0.0	44.807636	4
CAACGCA	8430	0.0	44.052216	5
ATCAACG	8785	0.0	42.42719	3
AACGCAG	8825	0.0	42.203636	6
TATCAAC	9225	0.0	40.27541	2
ACGCAGA	10495	0.0	35.20378	7
GTACATG	10810	0.0	33.97662	1
CGCAGAG	10985	0.0	33.524376	8
TACATGG	11165	0.0	32.903957	2
ACATGGG	11465	0.0	31.990301	3
GCAGAGT	11750	0.0	30.936665	9
CATGGGG	9395	0.0	29.849413	4
AGAGTAC	10875	0.0	28.393127	10-11
GTGGTAT	1050	0.0	27.256937	1
ATGGGGG	5255	0.0	26.16074	5
CAGAGTA	11540	0.0	25.880524	10-11
GAGTACT	6295	0.0	24.809258	12-13
AGTACTT	6675	0.0	24.377337	12-13
>>END_MODULE