FastQCFastQC Report
Mon 15 Aug 2016
SRR2921499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535263
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.13014056874945856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.10232774449719689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.070.8325961
GTATCAA35900.059.8506361
TCAACGC43800.048.2230384
CAACGCA45000.046.804875
AACGCAG47450.044.6345986
TATCAAC48800.043.768412
ATCAACG48600.043.581273
GTACATG79250.038.9784131
ACGCAGA57100.037.2996527
TACATGG85150.036.1236882
ACATGGG84750.035.872973
CGCAGAG60500.034.8078738
CATGGGG70250.034.3858684
GCAGAGT65350.031.4963389
TCTATAC4450.028.076663
ATGGGGA25750.027.2649755
ATGGGGG38750.027.1770235
CAGAGTA62950.026.17747310-11
AGAGTAC60150.025.56634310-11
ACTATAC4000.022.3109173
Produced by FastQC (version 0.11.5)