FastQCFastQC Report
Mon 15 Aug 2016
SRR2921499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535263
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.21924582302836715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.19227975923343427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.10799452601932048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.082.134981
GTATCAA47700.056.7396241
TCAACGC65050.040.9219064
CAACGCA65900.040.0929075
ATCAACG68100.039.1765753
TATCAAC68600.038.8910332
AACGCAG69050.038.255186
GTACATG77150.033.9216961
TACATGG81150.032.362772
ACGCAGA81650.032.2039227
ACATGGG80750.031.7118453
CGCAGAG85850.030.5591268
GTGGTAT9200.030.455031
CATGGGG68550.029.1895524
GCAGAGT91150.028.5864439
ATGGGGG37600.027.0631475
AGAGTAC84000.026.87665210-11
CAGAGTA89250.025.82891310-11
GAGTACT50350.024.51745812-13
GTACTTT53400.023.28498614-15
Produced by FastQC (version 0.11.5)