##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1535263
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201282125603235	31.0	31.0	34.0	28.0	34.0
2	31.265195604922415	33.0	31.0	34.0	28.0	34.0
3	31.346241653710145	33.0	31.0	34.0	28.0	34.0
4	34.83834756650815	37.0	35.0	37.0	32.0	37.0
5	34.49928579012195	37.0	35.0	37.0	30.0	37.0
6	34.471148591479114	37.0	35.0	37.0	30.0	37.0
7	34.306346209086	37.0	35.0	37.0	30.0	37.0
8	34.284564273352515	37.0	35.0	37.0	30.0	37.0
9	35.82779432579304	39.0	37.0	39.0	30.0	39.0
10-11	35.70364393592499	39.0	37.0	39.0	30.0	39.0
12-13	35.5993139286233	39.0	35.0	39.0	30.0	39.0
14-15	36.773573973970585	40.0	37.0	41.0	30.0	41.0
16-17	36.75941841886374	40.0	37.0	41.0	30.0	41.0
18-19	36.78776567923542	40.0	37.0	41.0	30.0	41.0
20-21	36.7799709235486	40.0	37.0	41.0	30.0	41.0
22-23	36.72959551555662	40.0	37.0	41.0	30.0	41.0
24-25	36.66382925922139	40.0	36.5	41.0	30.0	41.0
26-27	36.53885458061583	40.0	36.0	41.0	29.5	41.0
28-29	36.35571983432155	40.0	36.0	41.0	28.5	41.0
30-31	36.30044559140681	40.0	36.0	41.0	29.0	41.0
32-33	36.16563774415198	40.0	35.0	41.0	29.0	41.0
34-35	36.03921966464378	40.0	35.0	41.0	28.0	41.0
36-37	35.90034476177698	40.0	35.0	41.0	27.5	41.0
38-39	35.72896337630751	40.0	35.0	41.0	27.0	41.0
40-41	35.524061675426296	39.5	35.0	41.0	26.0	41.0
42-43	35.34772087909368	39.0	35.0	41.0	25.5	41.0
44-45	35.116818421338884	39.0	35.0	41.0	24.5	41.0
46-47	34.81173062856331	39.0	34.5	41.0	23.5	41.0
48-49	34.53509594121658	39.0	34.0	41.0	22.5	41.0
50-51	34.3104966380353	39.0	34.0	41.0	22.0	41.0
52-53	34.109818643450666	38.0	34.0	41.0	20.5	41.0
54-55	33.895539070504526	38.0	33.0	40.0	20.0	41.0
56-57	33.63609296908738	38.0	33.0	40.0	19.0	41.0
58-59	33.49005870655386	37.0	33.0	40.0	19.0	41.0
60-61	33.37126798470359	37.0	33.0	40.0	18.5	41.0
62-63	33.0993139286233	36.5	33.0	40.0	18.0	41.0
64-65	32.80942385767129	36.0	33.0	39.0	17.5	41.0
66-67	32.50445428568265	35.5	32.5	39.0	15.0	41.0
68-69	32.18248046100245	35.0	32.0	39.0	13.0	41.0
70-71	31.87745552390698	35.0	32.0	37.5	12.5	40.0
72-73	31.561101908923746	35.0	32.0	37.0	9.0	39.5
74-75	31.273167528951063	35.0	32.0	36.5	7.5	39.0
76-77	30.980386422391476	35.0	31.5	36.0	7.0	39.0
78-79	30.705575201121892	35.0	31.0	36.0	7.0	37.5
80-81	30.484264259608942	35.0	31.0	35.0	3.5	37.0
82-83	30.298343997087144	35.0	31.0	35.0	2.0	36.5
84-85	30.108360587078565	35.0	31.0	35.0	2.0	36.0
86-87	29.962111377659724	35.0	31.0	35.0	2.0	36.0
88-89	29.851174359051186	35.0	31.0	35.0	2.0	36.0
90-91	29.750303042540594	35.0	31.0	35.0	2.0	35.0
92-93	29.802642609116482	35.0	31.0	35.0	2.0	35.0
94-95	29.772068694419133	35.0	31.0	35.0	2.0	35.0
96-97	29.749390169632175	35.0	31.0	35.0	2.0	35.0
98-99	29.7071267268214	35.0	31.0	35.0	2.0	35.0
100-101	29.660155295867874	35.0	31.0	35.0	2.0	35.0
102-103	28.788650218236224	33.5	29.5	35.0	2.0	35.0
104-105	29.48394118792676	34.5	30.5	35.0	2.0	35.0
106-107	29.587302957213193	35.0	31.0	35.0	2.0	35.0
108-109	29.590519018565548	35.0	31.0	35.0	2.0	35.0
110-111	29.554901668313505	35.0	31.0	35.0	2.0	35.0
112-113	29.50795010366302	35.0	31.0	35.0	2.0	35.0
114-115	29.445919037975905	35.0	31.0	35.0	2.0	35.0
116-117	29.37292209868928	35.0	31.0	35.0	2.0	35.0
118-119	29.28042100929938	35.0	31.0	35.0	2.0	35.0
120-121	29.15932840171358	35.0	31.0	35.0	2.0	35.0
122-123	29.043426435731206	35.0	31.0	35.0	2.0	35.0
124-125	28.147834279859545	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26602.0
3	9904.0
4	5639.0
5	4510.0
6	4906.0
7	5873.0
8	6500.0
9	7384.0
10	8232.0
11	9232.0
12	9819.0
13	11571.0
14	14743.0
15	12708.0
16	7173.0
17	6047.0
18	5318.0
19	4738.0
20	4486.0
21	4771.0
22	5506.0
23	7402.0
24	10323.0
25	14631.0
26	18206.0
27	19480.0
28	23049.0
29	27539.0
30	31241.0
31	37499.0
32	47383.0
33	60357.0
34	84118.0
35	132741.0
36	266090.0
37	437551.0
38	141655.0
39	336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.17183790026548	17.620582594422647	12.593750957806934	24.61382854750494
2	17.10082188996914	19.626873157456608	37.60130324730564	25.671001705268615
3	19.322783655600144	24.22493094282587	29.104341481211236	27.347943920362745
4	13.373805706539448	15.52288220649383	35.29731878674122	35.805993300225495
5	14.184562582171797	37.21064777111644	33.29076796182668	15.314021684885079
6	33.673583730523774	35.097736209956295	16.4490262862325	14.779653773287436
7	29.629663409139624	31.71785963469921	20.129849401534454	18.522627554626716
8	28.16981594196857	33.71370429816151	18.705574894734646	19.41090486513528
9	27.750738020359723	14.478487325330839	18.551343548803143	39.219431105506295
10-11	26.55172986583903	25.019485276036253	26.43043748312443	21.998347375000286
12-13	26.930307331438662	22.588577387842363	27.064753843678574	23.416361437040408
14-15	24.242553531037338	23.50669209211133	24.696661526210626	27.55409285064071
16-17	23.99649533090786	26.603272110195135	25.17283946596139	24.227393092935618
18-19	23.97872159560023	25.29658738453652	27.530344329607477	23.19434669025578
20-21	25.044892806109004	24.237327167175174	27.424398820751566	23.293381205964252
22-23	24.857608248510616	23.905789376485842	27.36514270768904	23.8714596673145
24-25	24.25636632508811	24.37014260307616	27.384709093633347	23.988781978202383
26-27	23.96478545923095	24.439378556964687	27.49395888032378	24.101877103480575
28-29	24.167565400166318	24.571107870495084	27.393690223093287	23.867636506245315
30-31	24.39173201158097	24.46570923011573	27.30922281899133	23.83333593931197
32-33	24.063535700151625	24.54410871360239	27.25240506370345	24.139950522542534
34-35	24.24218042516597	24.59584476165037	27.294990651039463	23.866984162144202
36-37	24.431447849943076	24.424053797432276	27.22053657368727	23.923961778937375
38-39	24.365253716096483	24.681323878916757	27.0205297391957	23.932892665791066
40-41	24.394248573787657	24.543522479842487	27.097399350855568	23.964829595514292
42-43	24.3543751490129	24.591818654395603	26.87906080508005	24.174745391511443
44-45	24.325683599793877	24.747214819032457	26.685326830831812	24.241774750341857
46-47	24.473124555990115	24.82644323367208	26.373071559817106	24.327360650520692
48-49	24.582023415483953	25.217950459452567	25.80690450898399	24.393121616079487
50-51	24.514881168565296	25.418718634172212	25.73832556845702	24.32807462880547
52-53	24.67022947611326	25.295925354299314	25.809857250912255	24.22398791867517
54-55	24.51353806694717	25.231296822480196	25.94345292287587	24.311712187696767
56-57	24.465845410738176	25.33396572016036	25.759100360245498	24.44108850885597
58-59	24.453672104565573	25.15018744317991	25.978396721560316	24.4177437306942
60-61	24.633912441276472	25.235333858432895	26.07959753659247	24.05115616369816
62-63	24.46889670979166	25.261154016045158	26.064128507190613	24.20582076697257
64-65	24.43238358989794	25.023298144259243	26.113530773908817	24.430787491934005
66-67	24.477149420824645	25.206399315210604	25.950729950187956	24.365721313776795
68-69	24.457550703959242	25.247900501013103	25.867619177921547	24.42692961710611
70-71	24.300303187837045	25.118988522640066	26.08858374668246	24.49212454284043
72-73	24.581016141110695	25.383657976401853	25.652584196610604	24.382741685876848
74-75	24.456448270931062	25.54887755391217	25.61969310870839	24.374981066448377
76-77	24.481972621158604	25.230643351960257	25.623430836960786	24.663953189920353
78-79	24.364485393694746	25.36041352632705	25.743036794056813	24.532064285921386
80-81	24.413879775154992	25.56618438437343	25.454354743296737	24.565581097174842
82-83	24.585007066238948	25.37110375662043	25.571870278249243	24.47201889889139
84-85	24.46484176514273	25.473564551222754	25.397769468294403	24.663824215340117
86-87	24.227452035465564	25.566807692044613	25.622277601654392	24.58346267083543
88-89	24.388331512935036	25.387373482861165	25.514806792925103	24.709488211278696
90-91	24.357951686163638	25.38696181112557	25.574152276691414	24.680934226019378
92-93	24.364904235274576	25.486396214032247	25.524210293181255	24.624489257511925
94-95	24.33680661408542	25.31729839728808	25.55007725639482	24.795817732231683
96-97	24.318974271456682	25.362928203822122	25.533066574779788	24.785030949941408
98-99	24.241072155547233	25.365825856144863	25.36738914491361	25.02571284339429
100-101	24.15702468302213	25.452817387458076	25.544245638755154	24.845912290764637
102-103	24.203851735665097	25.245374556085054	25.654082454188497	24.89669125406135
104-105	24.171448857159607	25.46062102253112	25.43765999439816	24.930270125911115
106-107	24.240224431625734	25.368798428405427	25.454289056678498	24.936688083290342
108-109	24.261575886766934	25.34486285618238	25.344732559457654	25.048828697593027
110-111	24.20178915952232	25.52115426452829	25.427390789489113	24.84966578646028
112-113	24.168190437601826	25.43802850206206	25.47665747952762	24.917123580808497
114-115	23.964743907078052	25.665831175124932	25.444951355632796	24.924473562164216
116-117	23.94378858379046	25.611038261831386	25.383338121192107	25.061835033186046
118-119	23.991113328834192	25.376976733884522	25.417820765705084	25.214089171576205
120-121	23.82059110329163	25.367851813154925	25.674066585591294	25.13749049796215
122-123	23.79480001185472	25.557929032566935	25.430490795168353	25.21678016040999
124-125	23.756393329730493	25.54015716328192	25.417575927560126	25.28587357942746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	40.0
2	88.5
3	131.5
4	101.0
5	93.5
6	183.5
7	403.0
8	661.5
9	814.5
10	739.5
11	613.0
12	664.5
13	795.5
14	952.5
15	1113.0
16	1283.5
17	1470.0
18	1666.5
19	1820.5
20	1987.5
21	2268.5
22	2521.0
23	2815.5
24	3264.5
25	3814.5
26	4643.0
27	5601.5
28	6389.0
29	7666.5
30	9502.0
31	11012.0
32	12919.5
33	15154.5
34	17468.0
35	19828.5
36	22540.5
37	25643.5
38	27850.0
39	30295.0
40	32696.5
41	34513.0
42	37071.0
43	40772.0
44	44630.5
45	48039.0
46	51510.0
47	54687.5
48	58774.5
49	62619.5
50	63242.0
51	63653.5
52	63948.5
53	64377.5
54	65279.0
55	63986.0
56	60710.0
57	55574.5
58	51521.5
59	47897.5
60	42672.5
61	37376.5
62	31737.5
63	25038.0
64	18996.5
65	14823.5
66	11496.0
67	8836.0
68	7007.0
69	5566.0
70	4504.5
71	3606.5
72	2793.5
73	2435.0
74	1982.0
75	1556.5
76	1132.5
77	628.0
78	331.0
79	185.0
80	114.0
81	43.5
82	21.0
83	13.0
84	7.5
85	5.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11802538066767715
2	0.001367843815685
3	0.019736032197740713
4	0.05751457567856452
5	0.024034969904179283
6	0.020517657235275
7	0.016544396627809044
8	0.0061878648804797615
9	0.004885156484589285
10-11	0.008435036863390833
12-13	0.005210833583561904
14-15	0.003615015798596071
16-17	0.01094275052548
18-19	0.005959890911198929
20-21	0.014166953805308928
22-23	0.009737745259281309
24-25	0.015176552812124048
26-27	0.010323964037432022
28-29	0.015143985102226786
30-31	0.02155982395198738
32-33	0.014362360064692499
34-35	0.021690094791576426
36-37	0.015990745559555593
38-39	0.020778198914453094
40-41	0.01077991197599369
42-43	0.010486802586918333
44-45	0.017195750825754287
46-47	0.016055880979350116
48-49	0.022048339600446307
50-51	0.01644669349811726
52-53	0.021624959371781903
54-55	0.01800994357318583
56-57	0.021983204180651784
58-59	0.017228318535651547
60-61	0.020582792655069522
62-63	0.022471719829110712
64-65	0.017553995634624165
66-67	0.012538568310445832
68-69	0.02429551158335738
70-71	0.015697636170480235
72-73	0.017553995634624165
74-75	0.01885670403051464
76-77	0.013613302737055475
78-79	0.030190267074761783
80-81	0.02208090731034357
82-83	0.009542338999897738
84-85	0.013222490218288332
86-87	0.012799109989623927
88-89	0.022308881279624403
90-91	0.012733974569829404
92-93	0.007848818085240118
94-95	0.007490573276370238
96-97	0.00273568763137
98-99	0.0024751459521919045
100-101	0.011691807853117023
102-103	0.0038429897678769046
104-105	0.0034521772491097617
106-107	4.5594793856166665E-4
108-109	0.01986630303732976
110-111	0.0011073021365069046
112-113	0.009509771290000475
114-115	0.0025402813719864283
116-117	0.008467604573288094
118-119	0.01009599006815119
120-121	0.004298937706438572
122-123	6.187864880479761E-4
124-125	0.02361158967551488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1535263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.88033638184782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12799305807938	47.179631848620815
2	10.30926253726231	12.140256920855734
3	3.540309717295244	6.253638811508056
4	1.7220627865323967	4.055825445667589
5	1.0138262617743863	2.984721566301358
6	0.6881657915541093	2.4311659975911915
7	0.4749815341022281	1.9576950752173718
8	0.34716747419371397	1.635307012908988
9	0.26475369490655826	1.4029907952991731
>10	1.425264756154296	14.825798004600177
>50	0.06510963557592891	2.56654981060408
>100	0.020547451359977317	1.9648063858008817
>500	2.2212048382438626E-4	0.08004059628585733
>1k	3.3318072573657937E-4	0.5215717287385605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3366	0.21924582302836715	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2952	0.19227975923343427	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1658	0.10799452601932048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.51354197945238E-5	0.0
3	0.0	0.0	0.0	6.51354197945238E-5	0.0
4	0.0	0.0	0.0	1.302708395890476E-4	0.0
5	0.0	0.0	0.0	1.302708395890476E-4	0.0
6	0.0	0.0	0.0	1.302708395890476E-4	6.51354197945238E-5
7	0.0	0.0	0.0	1.9540625938357142E-4	6.51354197945238E-5
8	0.0	0.0	0.0	1.9540625938357142E-4	1.302708395890476E-4
9	0.0	0.0	0.0	1.9540625938357142E-4	1.302708395890476E-4
10-11	0.0	0.0	0.0	1.9540625938357142E-4	1.302708395890476E-4
12-13	0.0	0.0	0.0	1.9540625938357142E-4	2.605416791780952E-4
14-15	0.0	0.0	0.0	1.9540625938357142E-4	2.931093890753571E-4
16-17	0.0	0.0	0.0	3.25677098972619E-4	3.25677098972619E-4
18-19	0.0	0.0	0.0	3.25677098972619E-4	3.25677098972619E-4
20-21	0.0	0.0	0.0	3.25677098972619E-4	3.9081251876714283E-4
22-23	0.0	0.0	0.0	3.9081251876714283E-4	4.885156484589285E-4
24-25	0.0	0.0	0.0	4.5594793856166665E-4	5.862187781507142E-4
26-27	0.0	0.0	0.0	5.210833583561904E-4	5.862187781507142E-4
28-29	0.0	0.0	0.0	8.141927474315475E-4	5.862187781507142E-4
30-31	0.0	0.0	0.0	0.002279739692808333	6.187864880479761E-4
32-33	0.0	0.0	0.0	0.006546109689349643	6.51354197945238E-4
34-35	0.0	0.0	0.0	0.01218032350157595	6.51354197945238E-4
36-37	0.0	0.0	0.0	0.020126844716507858	6.51354197945238E-4
38-39	0.0	0.0	0.0	0.03719232470267309	6.51354197945238E-4
40-41	0.0	0.0	0.0	0.05683065377072202	7.164896177397619E-4
42-43	0.0	0.0	0.0	0.07425437856575715	7.164896177397619E-4
44-45	0.0	0.0	0.0	0.0941206816030869	7.164896177397619E-4
46-47	0.0	0.0	0.0	0.11649469830250582	7.164896177397619E-4
48-49	0.0	0.0	0.0	0.1409530484353495	7.164896177397619E-4
50-51	0.0	0.0	0.0	0.17127358634970036	7.164896177397619E-4
52-53	0.0	0.0	0.0	0.19924924915144832	7.164896177397619E-4
54-55	0.0	0.0	0.0	0.2307422246221006	7.164896177397619E-4
56-57	0.0	0.0	0.0	0.2666318409288832	7.490573276370238E-4
58-59	0.0	0.0	0.0	0.3093606763140908	8.141927474315476E-4
60-61	0.0	0.0	0.0	0.35332708467539437	8.467604573288095E-4
62-63	0.0	0.0	0.0	0.40149472761344474	9.118958771233333E-4
64-65	0.0	0.0	0.0	0.4468615475003306	9.118958771233333E-4
66-67	0.0	0.0	0.0	0.49978407608338116	9.118958771233333E-4
68-69	0.0	0.0	0.0	0.5581779799291717	9.118958771233333E-4
70-71	0.0	0.0	0.0	0.6247463789591751	9.77031296917857E-4
72-73	0.0	0.0	0.0	0.6964279084430485	0.0011724375563014284
74-75	0.0	3.25677098972619E-5	0.0	0.7664159170122644	0.0011724375563014284
76-77	0.0	6.51354197945238E-5	0.0	0.8463696448100423	0.0011724375563014284
78-79	0.0	6.51354197945238E-5	0.0	0.9402297847339511	0.0011724375563014284
80-81	0.0	6.51354197945238E-5	0.0	1.0465959252584085	0.0012050052661986904
82-83	0.0	6.51354197945238E-5	0.0	1.163676842339065	0.001302708395890476
84-85	0.0	6.51354197945238E-5	0.0	1.2736905663720157	0.001335276105787738
86-87	0.0	6.51354197945238E-5	0.0	1.398327192148837	0.0014329792354795237
88-89	0.0	6.51354197945238E-5	0.0	1.5413645740176114	0.0015306823651713094
90-91	0.0	6.51354197945238E-5	0.0	1.6970382273265232	0.0015632500750685713
92-93	0.0	6.51354197945238E-5	0.0	1.8656412614646483	0.0016283854948630952
94-95	3.25677098972619E-5	6.51354197945238E-5	0.0	2.030140764155718	0.0016283854948630952
96-97	6.51354197945238E-5	6.51354197945238E-5	0.0	2.20187029844398	0.0017260886245548809
98-99	6.51354197945238E-5	6.51354197945238E-5	0.0	2.3894603074522083	0.0018237917542466666
100-101	6.51354197945238E-5	6.51354197945238E-5	0.0	2.614926563070953	0.0018237917542466666
102-103	6.51354197945238E-5	6.51354197945238E-5	0.0	2.865241981341308	0.001986630303732976
104-105	6.51354197945238E-5	6.51354197945238E-5	0.0	3.119367821669642	0.002019198013630238
106-107	6.51354197945238E-5	6.51354197945238E-5	0.0	3.3761967819194494	0.0020843334334247616
108-109	6.51354197945238E-5	6.51354197945238E-5	0.0	3.6506123055137785	0.0022146042730138093
110-111	6.51354197945238E-5	6.51354197945238E-5	0.0	3.9603637943466365	0.002279739692808333
112-113	6.51354197945238E-5	6.51354197945238E-5	0.0	4.287083060035968	0.002279739692808333
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2475	0.0	82.13498	1
GTATCAA	4770	0.0	56.739624	1
TCAACGC	6505	0.0	40.921906	4
CAACGCA	6590	0.0	40.092907	5
ATCAACG	6810	0.0	39.176575	3
TATCAAC	6860	0.0	38.891033	2
AACGCAG	6905	0.0	38.25518	6
GTACATG	7715	0.0	33.921696	1
TACATGG	8115	0.0	32.36277	2
ACGCAGA	8165	0.0	32.203922	7
ACATGGG	8075	0.0	31.711845	3
CGCAGAG	8585	0.0	30.559126	8
GTGGTAT	920	0.0	30.45503	1
CATGGGG	6855	0.0	29.189552	4
GCAGAGT	9115	0.0	28.586443	9
ATGGGGG	3760	0.0	27.063147	5
AGAGTAC	8400	0.0	26.876652	10-11
CAGAGTA	8925	0.0	25.828913	10-11
GAGTACT	5035	0.0	24.517458	12-13
GTACTTT	5340	0.0	23.284986	14-15
>>END_MODULE