FastQCFastQC Report
Mon 15 Aug 2016
SRR2921498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395063
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.074.1151051
GTATCAA33350.057.9892431
TCAACGC41950.044.6713874
CAACGCA42650.044.0776985
AACGCAG44950.041.822336
TATCAAC47550.040.2847482
ATCAACG47900.039.2452283
ACGCAGA53000.035.355297
GTACATG76200.034.4384421
CGCAGAG55550.033.5169458
TACATGG80400.031.668172
ACATGGG79600.031.538043
GCAGAGT61200.029.839479
CATGGGG67050.028.569854
AGAGTAC55350.026.70625110-11
CAGAGTA59650.025.62871410-11
ATGGGGG35200.024.3372065
ATGGGGA26000.023.1099455
GTATAGA5250.020.4020181
GAGTACA55600.020.32708712-13
Produced by FastQC (version 0.11.5)