FastQCFastQC Report
Mon 15 Aug 2016
SRR2921498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395063
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.1813538169960783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.16687418417662858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.079.847281
GTATCAA43000.056.712631
TCAACGC56650.042.469414
CAACGCA57600.041.7360085
ATCAACG60150.039.99823
AACGCAG60150.039.9580546
TATCAAC61500.039.3138582
ACGCAGA72150.033.228587
CGTATAC902.3810615E-433.084323
GTACATG73800.032.6399841
CGCAGAG76050.031.36818
TACATGG76600.030.786562
ACATGGG77200.030.1615873
GTGGTAT7500.029.4147661
GCAGAGT82100.028.4044239
CATGGGG63100.027.7467884
AGAGTAC74750.026.34314510-11
CAGAGTA78000.026.313310-11
GAGTACT42700.023.89433112-13
GTATATA4150.022.9877681
Produced by FastQC (version 0.11.5)