FastQCFastQC Report
Mon 15 Aug 2016
SRR2921497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647191
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.11468008263765404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.073.2071
GTATCAA35550.062.7662121
TCAACGC47350.046.8644944
CAACGCA49500.045.0693365
TATCAAC51600.043.1172072
AACGCAG51950.042.828026
ATCAACG53050.042.0521053
ACGCAGA60600.036.908877
GTACATG86400.035.7427441
CGCAGAG64350.034.38518
TACATGG89250.034.0597422
ACATGGG91250.033.5098233
CATGGGG76950.031.6205774
GCAGAGT69650.031.0009239
GTATAGG4850.026.9907671
AGAGTAC64200.026.86903610-11
CAGAGTA67650.025.01517910-11
ATGGGGG41900.023.9953885
ATGGGGA29950.023.8363385
GAGTACT28550.021.1486212-13
Produced by FastQC (version 0.11.5)