##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921497_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647191
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.62024501105215	31.0	30.0	34.0	25.0	34.0
2	29.630009513165138	31.0	30.0	34.0	25.0	34.0
3	29.786705366894306	31.0	30.0	34.0	25.0	34.0
4	33.150624912350786	37.0	35.0	37.0	26.0	37.0
5	33.19732077215089	37.0	35.0	37.0	26.0	37.0
6	33.19074169297914	37.0	35.0	37.0	26.0	37.0
7	33.039297203542276	37.0	35.0	37.0	26.0	37.0
8	33.00427454982452	37.0	35.0	37.0	25.0	37.0
9	34.434540378134656	39.0	35.0	39.0	25.0	39.0
10-11	34.306724599636595	39.0	35.0	39.0	25.0	39.0
12-13	34.144445908215864	39.0	35.0	39.0	22.5	39.0
14-15	35.26188978691603	40.0	36.0	41.0	23.0	41.0
16-17	35.18138728295626	40.0	35.0	41.0	22.5	41.0
18-19	35.186839898955256	40.0	35.0	41.0	22.5	41.0
20-21	35.21530806081384	40.0	35.0	41.0	23.0	41.0
22-23	35.17418653938736	40.0	35.0	41.0	23.0	41.0
24-25	35.12720230987178	40.0	35.0	41.0	23.0	41.0
26-27	35.04483420562643	40.0	35.0	41.0	23.0	41.0
28-29	34.99709596519165	40.0	35.0	41.0	23.0	41.0
30-31	34.88965517660065	40.0	35.0	41.0	23.0	41.0
32-33	34.77890906397619	40.0	34.5	41.0	22.0	41.0
34-35	34.608654673319606	39.0	34.0	41.0	20.5	41.0
36-37	34.45548937554904	39.0	34.0	41.0	19.0	41.0
38-39	34.322294135895596	39.0	34.0	41.0	18.0	41.0
40-41	34.16868292748079	39.0	33.5	41.0	16.0	41.0
42-43	33.9523528236859	39.0	33.0	41.0	15.0	41.0
44-45	33.736550588243865	38.5	33.0	41.0	13.5	41.0
46-47	33.470485511394855	38.0	33.0	40.5	9.0	41.0
48-49	33.305059643963574	38.0	33.0	40.0	8.0	41.0
50-51	33.099757101635454	38.0	33.0	40.0	7.0	41.0
52-53	32.90274230493003	38.0	32.0	40.0	4.0	41.0
54-55	32.696497552499984	37.5	32.0	40.0	2.0	41.0
56-57	32.4518723086758	37.0	32.0	40.0	2.0	41.0
58-59	32.20070683970469	36.5	31.5	40.0	2.0	41.0
60-61	31.88455497874867	36.0	31.0	39.5	2.0	41.0
62-63	31.565845430189942	35.5	31.0	39.0	2.0	41.0
64-65	31.249412181101036	35.0	31.0	39.0	2.0	40.5
66-67	31.202891771506764	35.0	31.0	39.0	2.0	40.0
68-69	30.955075337347033	35.0	31.0	37.5	2.0	40.0
70-71	30.650690174970602	35.0	31.0	37.0	2.0	39.5
72-73	30.329228668685055	35.0	30.0	37.0	2.0	39.0
74-75	30.035426978413554	35.0	30.0	36.0	2.0	39.0
76-77	29.765486212588584	35.0	30.0	36.0	2.0	38.0
78-79	29.501947557994185	35.0	30.0	35.0	2.0	37.0
80-81	29.274345840889126	35.0	30.0	35.0	2.0	37.0
82-83	29.092986484263207	34.5	30.0	35.0	2.0	36.0
84-85	28.9094628370359	34.0	29.0	35.0	2.0	36.0
86-87	28.763001072735342	34.0	29.0	35.0	2.0	36.0
88-89	28.647970089686016	34.0	29.0	35.0	2.0	35.0
90-91	28.545891460067473	34.0	29.0	35.0	2.0	35.0
92-93	28.448177837299987	34.0	29.0	35.0	2.0	35.0
94-95	28.38665491737145	34.0	29.0	35.0	2.0	35.0
96-97	28.289665861457475	34.0	29.0	35.0	2.0	35.0
98-99	28.22725628054063	34.0	29.0	35.0	2.0	35.0
100-101	28.162740082965485	34.0	29.0	35.0	2.0	35.0
102-103	27.185338858699446	32.5	26.0	34.5	2.0	35.0
104-105	28.00545565146968	34.0	28.5	35.0	2.0	35.0
106-107	28.331455186435576	34.0	29.0	35.0	2.0	35.0
108-109	28.372420988215694	34.0	29.0	35.0	2.0	35.0
110-111	28.31143807852277	34.0	29.0	35.0	2.0	35.0
112-113	28.240660008462893	34.0	29.0	35.0	2.0	35.0
114-115	28.118332664517958	34.0	29.0	35.0	2.0	35.0
116-117	28.05369717294473	34.0	29.0	35.0	2.0	35.0
118-119	27.892707342378632	34.0	29.0	35.0	2.0	35.0
120-121	27.72203527095522	34.0	29.0	35.0	2.0	35.0
122-123	27.592586409226374	34.0	29.0	35.0	2.0	35.0
124-125	26.750289735677285	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72912.0
3	12677.0
4	8323.0
5	7036.0
6	7355.0
7	8373.0
8	9159.0
9	9557.0
10	9940.0
11	10616.0
12	10654.0
13	11236.0
14	12484.0
15	10778.0
16	7542.0
17	6495.0
18	6091.0
19	5681.0
20	5628.0
21	5779.0
22	6660.0
23	9243.0
24	13121.0
25	17461.0
26	20846.0
27	22778.0
28	26457.0
29	31298.0
30	35479.0
31	42814.0
32	53783.0
33	69416.0
34	97341.0
35	153102.0
36	292425.0
37	413458.0
38	103077.0
39	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.02618761311324	16.851413232275384	12.199164328486924	24.923234826124453
2	16.873706192725592	19.192220973325632	37.852679186804366	26.081393647144406
3	19.393297181755802	23.79397244302483	28.709291522191926	28.10343885302744
4	13.489460391220465	14.955185939334168	35.01654528196816	36.538808387477204
5	14.591739319488608	36.761740849874045	33.10357308562143	15.542946745015923
6	33.97659103180576	33.95758058334745	17.114748403091962	14.951079981754829
7	29.95462708394445	31.121411267131755	20.329629285481335	18.59433236344246
8	28.33897823358045	32.8439062102405	19.30537665361568	19.511738902563376
9	28.01469605878423	14.436266472338616	18.537499240906055	39.011538227971094
10-11	26.770082207520772	24.573104206147665	26.53539010540041	22.121423480931153
12-13	26.83705138961386	22.291059933749395	26.771432380081333	24.10045629655541
14-15	24.35954895144815	23.334317147764068	24.139769665964636	28.166364234823142
16-17	24.53422373050991	26.410659259106307	24.57415435143562	24.48096265894816
18-19	24.62433415734841	25.44466445174653	26.227671114728405	23.70333027617665
20-21	25.690901392828053	24.404256055846762	25.89719229337045	24.007650257954744
22-23	25.4759099081588	23.978418533456434	25.84464138199135	24.70103017639341
24-25	24.96498685583332	24.552847750912346	25.770554940490563	24.711610452763768
26-27	24.59097593018545	24.547457239013884	25.932007587375992	24.929559243424666
28-29	24.651011933287293	24.65353257981187	25.89687943960258	24.798576047298262
30-31	24.9695172596844	24.583772036781546	25.725944752367784	24.72076595116627
32-33	24.637655189756856	24.647252520969865	25.65761460154979	25.057477687723495
34-35	24.9221407054708	24.699039209017588	25.774979350889126	24.603840734622487
36-37	25.054270845228178	24.55070907425539	25.651948648300625	24.743071432215803
38-39	25.030547170206734	24.674429277416294	25.55255981186041	24.742463740516556
40-41	25.04218248342766	24.581181692619978	25.711392854062577	24.665242969889785
42-43	24.92148928002177	24.639124032966606	25.558139845663412	24.881246841348208
44-45	24.835217902342578	24.692531275341455	25.529547187117508	24.942703635198466
46-47	24.9051899936034	24.772003423669364	25.46903845067419	24.85376813205304
48-49	25.07794179010107	24.869246687208193	25.151950807235817	24.900860715454918
50-51	25.007987230150064	25.107326507035445	25.06444449573549	24.820241767079008
52-53	25.118732723883735	24.868100905123462	25.166690508884038	24.846475862108765
54-55	24.90750607591623	24.891957561057527	25.27581152163172	24.924724841394518
56-57	24.89128388268377	24.922592909765623	25.165503586417167	25.020619621133445
58-59	25.02575200094262	24.763221283785835	25.14151453348185	25.069512181789694
60-61	25.022120790141873	24.976269873032432	25.32595143689487	24.67565789993083
62-63	24.823481574090973	25.03687368262183	25.27875046424247	24.860894279044725
64-65	24.8938290095131	24.795378855445595	25.31356313336291	24.997229001678388
66-67	24.8394216903469	24.984172184766855	25.2804445938408	24.895961531045444
68-69	24.94443452565303	24.97479817283717	25.19432734197847	24.88643995953133
70-71	24.8056746789893	24.803275507219837	25.32969631040788	25.061353503382982
72-73	25.002300070717688	25.100891880887005	25.032603312648384	24.864204735746924
74-75	24.814423763961297	25.199711762792802	25.12792575796638	24.857938715279516
76-77	24.898385277844074	24.891401448142638	25.112211924615934	25.098001349397357
78-79	24.860194654102276	25.030903380743315	25.135417688302624	24.97348427685178
80-81	24.887394943695195	25.179446992659383	24.966670117813656	24.96648794583177
82-83	25.002314961696115	25.051437689883784	25.055384509824698	24.890862838595407
84-85	24.815591812446492	25.132917195441106	24.98217853044223	25.06931246167018
86-87	24.660612671735375	25.240519848940572	25.082374386081984	25.016493093242065
88-89	24.68576435643745	25.10478569674101	25.06224900390967	25.147200942911873
90-91	24.671065014675328	25.104281649708376	25.087918386479025	25.136734949137267
92-93	24.843468167939236	25.164471137512717	25.016857741308584	24.975202953239464
94-95	24.803166817753755	25.02196480409581	25.086386800351924	25.088481577798515
96-97	24.81159707152754	24.9710990029156	25.14174239011246	25.0755615354444
98-99	24.71590262259379	25.04540791838809	24.959130596603735	25.279558862414387
100-101	24.713556165820787	25.15229139888294	25.075940782279616	25.058211653016656
102-103	24.689222557023676	24.971965521993784	25.195816351249693	25.14299556973284
104-105	24.688386799864485	25.109345421031477	25.00258033336693	25.199687445737105
106-107	24.66438216020648	25.113795257787164	25.006663481148266	25.215159100858088
108-109	24.653338470071667	25.115796715098337	25.001707692265658	25.229157122564338
110-111	24.648520756043517	25.239599058816104	25.062982882680778	25.048897302459604
112-113	24.674697814966255	25.244779212231844	25.086313644644303	24.994209328157602
114-115	24.47147059903895	25.49065858189189	25.040276640641295	24.99759417842787
116-117	24.482950707699313	25.534398360717947	24.942587181725038	25.0400637498577
118-119	24.66714261195365	25.21837489638148	24.969446990528567	25.145035501136302
120-121	24.367089860976847	25.347420785501697	25.17013434954402	25.11535500397744
122-123	24.463483737133068	25.37372211222184	25.03829602210223	25.124498128542854
124-125	24.394536669000022	25.56748811718829	24.977248263187963	25.060726950623724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	189.5
2	231.5
3	244.5
4	215.0
5	184.5
6	203.0
7	259.0
8	349.5
9	438.0
10	453.5
11	473.5
12	560.5
13	629.0
14	709.0
15	827.5
16	904.0
17	1004.0
18	1107.5
19	1175.0
20	1263.5
21	1430.0
22	1658.5
23	1927.5
24	2245.0
25	2756.0
26	3489.5
27	4294.5
28	5026.5
29	6199.0
30	7901.0
31	9403.0
32	11159.0
33	12990.5
34	14828.0
35	16941.0
36	19734.5
37	22854.0
38	25495.0
39	28469.0
40	31545.0
41	34497.0
42	37588.0
43	42047.0
44	47051.0
45	50760.5
46	55159.0
47	59770.5
48	64609.5
49	69496.5
50	71036.0
51	72097.5
52	72102.0
53	72225.0
54	73748.0
55	73355.0
56	70097.5
57	64271.0
58	59826.0
59	55370.0
60	49262.0
61	43491.5
62	37381.5
63	29471.5
64	22133.0
65	17237.5
66	13388.5
67	10330.5
68	8133.0
69	6394.0
70	5297.0
71	4265.5
72	3332.5
73	2855.5
74	2320.5
75	1733.5
76	1179.0
77	702.5
78	411.0
79	278.0
80	169.5
81	84.0
82	58.5
83	39.0
84	33.0
85	25.0
86	17.5
87	17.5
88	13.0
89	11.0
90	11.0
91	10.0
92	12.0
93	14.0
94	13.0
95	10.0
96	10.5
97	11.0
98	9.5
99	11.5
100	30.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037093451821919865
2	0.02452660316866714
3	0.025558663202992247
4	0.030233288064347122
5	0.03320804933975477
6	0.04419645323462792
7	0.03727558006327135
8	0.03472578468435051
9	0.029808322167860314
10-11	0.03433117349475562
12-13	0.034270464080971785
14-15	0.032722374029484136
16-17	0.03551500706354029
18-19	0.04741405216517089
20-21	0.05900955019788233
22-23	0.05287789940571555
24-25	0.06213608500774955
26-27	0.04686766744111642
28-29	0.0478390180616577
30-31	0.04659447507908919
32-33	0.0546384724054466
34-35	0.038307640097596456
36-37	0.049721009888956405
38-39	0.05867564842207127
40-41	0.04692837685490025
42-43	0.055791951267339365
44-45	0.05621691716382617
46-47	0.06095225143896488
48-49	0.046746248613548765
50-51	0.04935675340625343
52-53	0.05776500721531382
54-55	0.044408936182871325
56-57	0.042557299062464526
58-59	0.04352864968300579
60-61	0.03339017758110626
62-63	0.041980559631518144
64-65	0.04082708076962538
66-67	0.034270464080971785
68-69	0.02944406568515734
70-71	0.04793008218233344
72-73	0.030506480426374355
74-75	0.03827728539070454
76-77	0.03217598930542966
78-79	0.03196350635718626
80-81	0.02401057315150459
82-83	0.017939631773121634
84-85	0.018273533548932696
86-87	0.01757537529041866
88-89	0.022917803703395658
90-91	0.01268826748082038
92-93	0.019518076531501203
94-95	0.014448840480551435
96-97	0.011474079205143787
98-99	0.010715211532845917
100-101	0.01117053213622464
102-103	0.0067994543437889104
104-105	0.007103001412708058
106-107	0.009349249722709754
108-109	0.014054229290956543
110-111	0.0070726467058161435
112-113	0.008833219705547202
114-115	0.008772510291763371
116-117	0.007801159671222099
118-119	0.25164052013397353
120-121	0.3679901116506829
122-123	0.029110163909346277
124-125	0.0038854024821650917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1647191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.65937496944047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25057578386158	47.07448616419035
2	10.246700272622391	12.021300669824493
3	3.407303547222999	5.996108892337756
4	1.7319928689908286	4.063904765861199
5	1.0282069152067368	3.0156987492641827
6	0.6828123276407202	2.403200661650007
7	0.4715698245789941	1.9363393814976735
8	0.34085615948657316	1.5995527415973037
9	0.2828468326251372	1.4932456572488844
>10	1.4633086062027445	15.176355386826748
>50	0.07275544779044911	2.8964025834554192
>100	0.020654201918090597	2.0105120997048935
>500	2.0860592636368235E-4	0.10921248730457647
>1k	2.0860592636368235E-4	0.20367975923637816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1889	0.11468008263765404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	6.0709413783829565E-5
12-13	0.0	0.0	0.0	0.0	1.517735344595739E-4
14-15	0.0	0.0	0.0	0.0	2.1248294824340348E-4
16-17	0.0	0.0	0.0	0.0	2.4283765513531826E-4
18-19	0.0	0.0	0.0	0.0	3.035470689191478E-4
20-21	0.0	0.0	0.0	3.0354706891914783E-5	3.339017758110626E-4
22-23	0.0	0.0	0.0	6.0709413783829565E-5	3.642564827029774E-4
24-25	0.0	0.0	0.0	1.517735344595739E-4	4.2496589648680696E-4
26-27	0.0	0.0	0.0	2.7319236202723307E-4	4.2496589648680696E-4
28-29	0.0	0.0	0.0	0.00139631651702808	4.2496589648680696E-4
30-31	0.0	0.0	0.0	0.004765688982030621	4.2496589648680696E-4
32-33	0.0	0.0	0.0	0.010654502119062088	4.856753102706365E-4
34-35	0.0	0.0	0.0	0.018577080617851845	4.856753102706365E-4
36-37	0.0	0.0	0.0	0.031204638684888396	4.856753102706365E-4
38-39	0.0	0.0	0.0	0.05139051876801172	5.46384724054466E-4
40-41	0.0	0.0	0.0	0.0749457713161376	5.46384724054466E-4
42-43	0.0	0.0	0.0	0.09643690379561326	5.767394309463808E-4
44-45	0.0	0.0	0.0	0.12147953698144295	6.374488447302104E-4
46-47	0.0	0.0	0.0	0.15107537620105987	6.678035516221252E-4
48-49	0.0	0.0	0.0	0.18231036959284017	6.678035516221252E-4
50-51	0.0	0.0	0.0	0.21573090188083835	6.678035516221252E-4
52-53	0.0	0.0	0.0	0.24890859651370123	7.285129654059548E-4
54-55	0.0	0.0	0.0	0.2875197836802168	7.892223791897843E-4
56-57	0.0	0.0	0.0	0.3289539585876805	8.195770860816991E-4
58-59	0.0	0.0	0.0	0.3749716942358233	8.802864998655286E-4
60-61	0.0	0.0	0.0	0.42645327712451075	9.106412067574434E-4
62-63	0.0	0.0	0.0	0.47966507830603733	0.0010320600343251024
64-65	0.0	0.0	0.0	0.5322090759359419	0.0010320600343251024
66-67	0.0	0.0	0.0	0.5940416138747723	0.001092769448108932
68-69	0.0	0.0	0.0	0.6668625557084759	0.0011534788618927617
70-71	0.0	0.0	0.0	0.7445098959379939	0.0011534788618927617
72-73	0.0	0.0	0.0	0.8278335663562999	0.0012748976894604207
74-75	0.0	0.0	0.0	0.9083949584474418	0.0012748976894604207
76-77	0.0	0.0	0.0	1.0014321350711606	0.0012748976894604207
78-79	0.0	0.0	0.0	1.10843247686516	0.0014266712239199948
80-81	0.0	0.0	0.0	1.2300030779672788	0.0014570259308119096
82-83	0.0	0.0	0.0	1.3583427787062945	0.0014570259308119096
84-85	0.0	0.0	0.0	1.485377227048958	0.0014570259308119096
86-87	0.0	0.0	0.0	1.6211841856833846	0.0015177353445957392
88-89	0.0	0.0	0.0	1.7757503531770147	0.0015784447583795686
90-91	0.0	0.0	0.0	1.9481954430299826	0.0015784447583795686
92-93	0.0	0.0	0.0	2.130566522036607	0.0016391541721633982
94-95	0.0	0.0	0.0	2.321679756628102	0.0016998635859472278
96-97	0.0	0.0	0.0	2.5186818043566293	0.0017605729997310572
98-99	0.0	0.0	0.0	2.7298291454967885	0.0018819918272987165
100-101	0.0	0.0	0.0	2.972818574166566	0.0018819918272987165
102-103	0.0	0.0	0.0	3.238634742419064	0.0020034106548663757
104-105	0.0	0.0	0.0	3.522785153634278	0.0020034106548663757
106-107	0.0	0.0	0.0	3.8113977067626035	0.0020034106548663757
108-109	0.0	0.0	0.0	4.113669877992291	0.0020034106548663757
110-111	0.0	0.0	0.0	4.446023563751867	0.0020641200686502053
112-113	0.0	0.0	0.0	4.8062428704382185	0.0021248294824340345
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1715	0.0	73.207	1
GTATCAA	3555	0.0	62.766212	1
TCAACGC	4735	0.0	46.864494	4
CAACGCA	4950	0.0	45.069336	5
TATCAAC	5160	0.0	43.117207	2
AACGCAG	5195	0.0	42.82802	6
ATCAACG	5305	0.0	42.052105	3
ACGCAGA	6060	0.0	36.90887	7
GTACATG	8640	0.0	35.742744	1
CGCAGAG	6435	0.0	34.3851	8
TACATGG	8925	0.0	34.059742	2
ACATGGG	9125	0.0	33.509823	3
CATGGGG	7695	0.0	31.620577	4
GCAGAGT	6965	0.0	31.000923	9
GTATAGG	485	0.0	26.990767	1
AGAGTAC	6420	0.0	26.869036	10-11
CAGAGTA	6765	0.0	25.015179	10-11
ATGGGGG	4190	0.0	23.995388	5
ATGGGGA	2995	0.0	23.836338	5
GAGTACT	2855	0.0	21.14862	12-13
>>END_MODULE