FastQCFastQC Report
Mon 15 Aug 2016
SRR2921497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647191
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30190.18328172021338146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.16537244314715172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.081.775931
GTATCAA48750.059.444811
TCAACGC64950.044.2805254
CAACGCA66400.043.2885635
AACGCAG69400.041.5774356
TATCAAC69550.041.438712
ATCAACG69500.041.3815843
ACGCAGA83650.034.351327
GTACATG84850.033.1697731
CGCAGAG87250.032.8647738
TACATGG85900.032.0263022
ACATGGG87350.030.812033
GCAGAGT94100.029.9033999
CATGGGG73850.029.7523084
CAGAGTA91900.026.08786610-11
GTATAGG4600.025.9253431
TATACCG2308.267307E-925.874165
AGAGTAC88000.025.62156110-11
GAGTACT53000.024.63737712-13
GTACTTT55150.023.57010714-15
Produced by FastQC (version 0.11.5)