##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647191
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19413170664483	31.0	31.0	34.0	28.0	34.0
2	31.258368337369497	33.0	31.0	34.0	28.0	34.0
3	31.341309538480967	33.0	31.0	34.0	28.0	34.0
4	34.84519706579261	37.0	35.0	37.0	32.0	37.0
5	34.48913453266804	37.0	35.0	37.0	30.0	37.0
6	34.459102799857455	37.0	35.0	37.0	30.0	37.0
7	34.308162198554996	37.0	35.0	37.0	30.0	37.0
8	34.277551905031046	37.0	35.0	37.0	30.0	37.0
9	35.835676615523035	39.0	37.0	39.0	30.0	39.0
10-11	35.70690587794615	39.0	36.0	39.0	30.0	39.0
12-13	35.60574760304057	39.0	35.0	39.0	30.0	39.0
14-15	36.78323369906708	40.0	37.0	41.0	30.0	41.0
16-17	36.76001143765356	40.0	37.0	41.0	30.0	41.0
18-19	36.77762354213931	40.0	37.0	41.0	30.0	41.0
20-21	36.76704098067559	40.0	37.0	41.0	30.0	41.0
22-23	36.70831221151646	40.0	37.0	41.0	30.0	41.0
24-25	36.64085767831418	40.0	36.5	41.0	30.0	41.0
26-27	36.514838594917045	40.0	36.0	41.0	29.0	41.0
28-29	36.32773855612373	40.0	36.0	41.0	28.5	41.0
30-31	36.278435834095745	40.0	36.0	41.0	29.0	41.0
32-33	36.15235513064363	40.0	35.0	41.0	28.5	41.0
34-35	36.0310179572375	40.0	35.0	41.0	28.0	41.0
36-37	35.894528624792144	40.0	35.0	41.0	27.0	41.0
38-39	35.726113729373225	40.0	35.0	41.0	27.0	41.0
40-41	35.521895760722344	39.5	35.0	41.0	26.0	41.0
42-43	35.35012970566255	39.0	35.0	41.0	26.0	41.0
44-45	35.12719532828919	39.0	35.0	41.0	24.5	41.0
46-47	34.84321004667947	39.0	34.5	41.0	23.5	41.0
48-49	34.579490478032	39.0	34.0	41.0	23.0	41.0
50-51	34.34786038777531	39.0	34.0	41.0	22.0	41.0
52-53	34.138302419088006	38.0	34.0	40.5	22.0	41.0
54-55	33.9092294093399	38.0	33.0	40.0	20.5	41.0
56-57	33.64186788295953	37.5	33.0	40.0	20.0	41.0
58-59	33.482086473274805	37.0	33.0	40.0	20.0	41.0
60-61	33.353130875532955	37.0	33.0	40.0	20.0	41.0
62-63	33.075185269953515	36.0	33.0	40.0	19.0	41.0
64-65	32.76970430265828	36.0	33.0	39.0	18.0	41.0
66-67	32.45914924255899	35.0	32.5	39.0	17.5	41.0
68-69	32.13675402548945	35.0	32.0	38.5	15.5	40.5
70-71	31.827351230063787	35.0	32.0	37.0	13.5	40.0
72-73	31.513607711552577	35.0	32.0	37.0	12.5	39.0
74-75	31.230756178245265	35.0	32.0	36.0	10.5	39.0
76-77	30.94892061697763	35.0	31.5	36.0	9.0	38.5
78-79	30.681709042849313	35.0	31.0	35.5	7.0	37.0
80-81	30.470630303346727	35.0	31.0	35.0	7.0	37.0
82-83	30.29248065342756	35.0	31.0	35.0	7.0	36.0
84-85	30.111348957103335	35.0	31.0	35.0	3.5	36.0
86-87	29.978534669021382	35.0	31.0	35.0	2.0	36.0
88-89	29.873750524377563	35.0	31.0	35.0	2.0	35.5
90-91	29.78274984503922	35.0	31.0	35.0	2.0	35.0
92-93	29.84707905761991	35.0	31.0	35.0	2.0	35.0
94-95	29.820878088819086	35.0	31.0	35.0	2.0	35.0
96-97	29.80024022715034	35.0	31.0	35.0	2.0	35.0
98-99	29.760404227560738	35.0	31.0	35.0	2.0	35.0
100-101	29.711241744278595	35.0	31.0	35.0	2.0	35.0
102-103	28.82967488287636	33.5	29.5	35.0	2.0	35.0
104-105	29.538973318819735	34.5	31.0	35.0	2.0	35.0
106-107	29.642921191288686	35.0	31.0	35.0	2.0	35.0
108-109	29.645945430736326	35.0	31.0	35.0	2.0	35.0
110-111	29.614149482361185	35.0	31.0	35.0	2.0	35.0
112-113	29.566787033197727	35.0	31.0	35.0	2.0	35.0
114-115	29.497931023178246	35.0	31.0	35.0	2.0	35.0
116-117	29.422346285282035	35.0	31.0	35.0	2.0	35.0
118-119	29.328703532255822	35.0	31.0	35.0	2.0	35.0
120-121	29.201119967265484	35.0	31.0	35.0	2.0	35.0
122-123	29.077100044864256	35.0	31.0	35.0	2.0	35.0
124-125	28.158672552241967	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28962.0
3	10671.0
4	5916.0
5	4863.0
6	5361.0
7	6138.0
8	7314.0
9	7933.0
10	8817.0
11	9585.0
12	10204.0
13	11651.0
14	14733.0
15	12378.0
16	7040.0
17	6186.0
18	5469.0
19	4997.0
20	4833.0
21	5110.0
22	6041.0
23	7938.0
24	11275.0
25	15700.0
26	19345.0
27	21339.0
28	24730.0
29	29843.0
30	34652.0
31	41249.0
32	51549.0
33	66114.0
34	91834.0
35	147615.0
36	298532.0
37	467661.0
38	133398.0
39	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20860103160835	17.353595129583187	12.506640538099365	24.931163300709095
2	17.239790380118166	19.5907895471754	36.8656703732215	26.303749699484936
3	19.426744243943375	23.915430081475574	28.972222525827718	27.685603148753334
4	13.726705093884265	15.370947784299755	34.67038911354374	36.231958008272244
5	14.667452843078257	36.77896804022859	32.98285329814566	15.570725818547487
6	34.14038643582446	34.436276221281595	16.442376177745178	14.980961165148763
7	29.85621273787195	31.408964587202863	19.96354466780048	18.771278007124696
8	28.470316959491086	32.91110114084046	18.940647275637037	19.677934624031415
9	28.02187794829577	14.120796193154428	18.38048785226253	39.47683800628727
10-11	26.824486752820814	24.592059979849267	26.2294360375123	22.35401722981762
12-13	27.095437158362124	22.18960300718739	26.789903956632195	23.92505587781829
14-15	24.531722311163566	23.14809249656832	24.361185633635735	27.95899955863238
16-17	24.49609419113388	26.15210129238376	24.80294881587144	24.548855700610915
18-19	24.504799315164135	24.904954738906326	26.959948030186204	23.63029791574333
20-21	25.51873688339231	23.83662349639998	26.860748188151163	23.783891432056542
22-23	25.28739191307632	23.511159925539783	26.76023372639877	24.44121443498513
24-25	24.79092863901959	24.06982332267042	26.675705402385148	24.463542635924846
26-27	24.442708682758248	24.156439552740157	26.812185289895307	24.58866647460629
28-29	24.53155026639749	24.256621988797644	26.77402510663489	24.437802638169977
30-31	24.833441215010815	24.162318002800472	26.66989906889814	24.334341713290577
32-33	24.497681677801637	24.19230613935677	26.591655011555943	24.71835717128565
34-35	24.688938909915397	24.264920640028954	26.62644069360961	24.419699756446043
36-37	24.944852974660602	24.1443786464889	26.45757853403936	24.453189844811142
38-39	24.87809380333064	24.25897639781876	26.358334540175075	24.504595258675522
40-41	24.913670652514774	24.207857086261953	26.404470334731705	24.474001926491567
42-43	24.840297368834072	24.27376778601441	26.233428281125203	24.652506564026314
44-45	24.74111531188057	24.316443932782423	26.159337988688584	24.783102766648422
46-47	24.995787625912037	24.403960451347913	25.83055114247175	24.7697007802683
48-49	25.153338097982147	24.684473580184243	25.3733640748313	24.78882424700231
50-51	25.023975984852097	24.999779897574957	25.21954837411098	24.75669574346197
52-53	25.193317260121585	24.75180489659132	25.301372720292413	24.75350512299468
54-55	24.93125764879148	24.823874764293503	25.397099599522864	24.84776798739215
56-57	24.940272936513068	24.867984138362157	25.275576374891344	24.916166550233427
58-59	24.97274480117139	24.663442695166502	25.398050376654552	24.965762127007558
60-61	24.984561651080565	24.807104130069533	25.58633847297272	24.621995745877186
62-63	24.86826221496789	24.812093765484274	25.50336039134532	24.816283628202513
64-65	24.909755293394724	24.55694327137289	25.58371399547395	24.94958743975843
66-67	24.856322231643382	24.783007180017748	25.478847469173527	24.881823119165343
68-69	24.946138422432647	24.78349103177408	25.35289352653062	24.91747701926265
70-71	24.79858259464599	24.684584198820488	25.553370375475197	24.963462831058326
72-73	25.074480594059583	24.93446041682416	25.155298831657312	24.835760157458946
74-75	24.845163076676457	25.031817469733937	25.206449231098055	24.91657022249155
76-77	24.96577070409434	24.80581136030106	25.136476984251793	25.09194095135281
78-79	24.879995603355905	24.889529762517455	25.205584102366508	25.024890531760136
80-81	24.904871219023754	25.066483123958427	25.020668289363947	25.00797736765388
82-83	25.047896514991585	24.963624148890084	25.018389043863827	24.9700902922545
84-85	24.784036998549166	25.072260893931237	25.00480423756522	25.138897869954373
86-87	24.7150098938243	25.05150313876187	25.072571914653853	25.16091505275998
88-89	24.718980703908404	24.91568813901833	25.06008396574819	25.305247191325076
90-91	24.70165858065715	24.937575156475745	25.10925296322573	25.251513299641378
92-93	24.781656803655963	25.010397295064003	25.025454399740628	25.182491501539406
94-95	24.74636058825583	24.824559938921936	25.182376610622786	25.24670286219945
96-97	24.681190787696288	24.81311602803639	25.180448594386046	25.32524458988128
98-99	24.62267779243932	24.869829492039816	24.97731710789407	25.53017560762679
100-101	24.595697580484803	24.943966421443548	25.091810673140646	25.368525324931003
102-103	24.640444407343043	24.699850498212808	25.247892176579068	25.411812917865085
104-105	24.575541629886143	24.89527416313432	24.97960122829747	25.549582978682068
106-107	24.63084781231512	24.87638770716006	24.99006639503177	25.50269808549305
108-109	24.672366315802257	24.747568895217334	25.012948687977605	25.567116101002803
110-111	24.623674779729466	24.986446524633934	25.012551762741026	25.377326932895578
112-113	24.55171258119081	24.938738808752152	24.995051747188604	25.51449686286843
114-115	24.409169405485773	25.171303307558535	24.98243179209684	25.437095494858852
116-117	24.40279917112866	25.138899092260814	24.896558046797352	25.561743689813177
118-119	24.43268855383319	24.881498315445505	25.002474160955856	25.68333896976545
120-121	24.22478296412165	24.97514624631671	25.119427029530968	25.68064376003067
122-123	24.286256771807064	24.99543917067883	24.989671732602144	25.728632324911967
124-125	24.185285481408208	25.079662628200282	24.985509675346897	25.749542215044613
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	58.5
2	92.0
3	132.0
4	109.0
5	98.5
6	186.0
7	385.5
8	659.5
9	780.0
10	636.0
11	507.0
12	564.0
13	667.5
14	746.5
15	882.0
16	1026.0
17	1173.5
18	1341.5
19	1483.0
20	1713.5
21	1961.0
22	2188.5
23	2422.5
24	2705.5
25	3185.0
26	3864.0
27	4642.5
28	5392.0
29	6424.0
30	7847.0
31	9426.0
32	11244.5
33	12832.0
34	14520.5
35	16621.0
36	19347.5
37	22242.0
38	24772.0
39	27637.5
40	30855.5
41	33656.0
42	36400.5
43	41281.0
44	46500.0
45	50274.0
46	54771.5
47	59674.5
48	64638.0
49	69529.5
50	70998.0
51	71998.5
52	72991.5
53	73542.0
54	74780.0
55	74018.0
56	70519.0
57	64783.5
58	60295.5
59	55743.5
60	49546.5
61	43727.5
62	37413.0
63	29216.0
64	21814.5
65	17004.0
66	13005.0
67	9853.5
68	7848.0
69	6193.5
70	5062.5
71	4035.0
72	3232.5
73	2926.0
74	2294.5
75	1669.5
76	1153.5
77	634.0
78	339.0
79	200.5
80	106.0
81	45.5
82	26.5
83	12.0
84	6.0
85	7.0
86	8.0
87	5.0
88	3.0
89	2.5
90	1.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12099386167117232
2	0.0011534788618927617
3	0.01924488416947397
4	0.05840245606004404
5	0.024405184341099485
6	0.01784856765244589
7	0.014813096963254413
8	0.004371077792435728
9	0.003217598930542967
10-11	0.00634413374041019
12-13	0.0042800136717599845
14-15	0.0023069577237855234
16-17	0.009531377964061241
18-19	0.004917462516490194
20-21	0.011261596256900383
22-23	0.008317189688384649
24-25	0.012475784532576976
26-27	0.008468963222844223
28-29	0.013113233377307184
30-31	0.019730559479744607
32-33	0.012293656291225486
34-35	0.019912687721096094
36-37	0.0139631651702808
38-39	0.02006446125555567
40-41	0.009440313843385497
42-43	0.008833219705547202
44-45	0.016421896428525897
46-47	0.015632674049336114
48-49	0.02064120068650205
50-51	0.013993519877172716
52-53	0.021339358945016092
54-55	0.017028990566364192
56-57	0.019700204772852694
58-59	0.015632674049336114
60-61	0.019305593583257803
62-63	0.021794679548394813
64-65	0.016998635859472276
66-67	0.011504433912035702
68-69	0.022887448996503745
70-71	0.014448840480551435
72-73	0.01733253763528334
74-75	0.01803069589379738
76-77	0.012748976894604209
78-79	0.029170873323130102
80-81	0.020914393048529285
82-83	0.009136766774466348
84-85	0.012597203360144636
86-87	0.01238472041190123
88-89	0.019973397134879924
90-91	0.012779331601496123
92-93	0.0077708049643301835
94-95	0.007194065533383804
96-97	0.00279263303405616
98-99	0.0014873806377038242
100-101	0.011383015084468043
102-103	0.003247953637434882
104-105	0.0020034106548663757
106-107	2.73192362027233E-4
108-109	0.018577080617851845
110-111	7.285129654059548E-4
112-113	0.008802864998655287
114-115	0.0023980218444612676
116-117	0.008013642619465503
118-119	0.010199181515683367
120-121	0.0033693724650025406
122-123	7.588676722978696E-4
124-125	0.0244355390479914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1647191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.97763687248912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40661053416737	45.244010202909585
2	10.616238853523058	12.09776404695294
3	3.604646241990754	6.161526738897952
4	1.7658073291925658	4.024461151580555
5	1.0774785360235595	3.0696090381725787
6	0.7034833317683721	2.404969069400826
7	0.5021303454653508	2.0027140340607588
8	0.3573909865790591	1.6290635083841813
9	0.28516471818816086	1.4623210585593651
>10	1.5759495862066157	15.935363699361051
>50	0.0805039727183403	3.10522945754516
>100	0.023954012028490665	2.298633222319286
>500	3.2077607422902353E-4	0.12093323136495258
>1k	3.2077607422902353E-4	0.4434015404907431
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3019	0.18328172021338146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2724	0.16537244314715172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.0709413783829565E-5
7	0.0	0.0	0.0	0.0	6.0709413783829565E-5
8	0.0	0.0	0.0	0.0	6.0709413783829565E-5
9	0.0	0.0	0.0	0.0	6.0709413783829565E-5
10-11	0.0	0.0	0.0	0.0	6.0709413783829565E-5
12-13	0.0	0.0	0.0	0.0	9.106412067574435E-5
14-15	0.0	0.0	0.0	0.0	1.821282413514887E-4
16-17	0.0	0.0	0.0	0.0	1.821282413514887E-4
18-19	0.0	0.0	0.0	0.0	1.821282413514887E-4
20-21	0.0	0.0	0.0	3.0354706891914783E-5	1.821282413514887E-4
22-23	0.0	0.0	0.0	1.2141882756765913E-4	2.4283765513531826E-4
24-25	0.0	0.0	0.0	2.1248294824340348E-4	3.642564827029774E-4
26-27	0.0	0.0	0.0	3.035470689191478E-4	5.160300171625513E-4
28-29	0.0	0.0	0.0	0.00139631651702808	5.767394309463808E-4
30-31	0.0	0.0	0.0	0.004704979568246791	6.070941378382957E-4
32-33	0.0	0.0	0.0	0.010745566239737832	6.070941378382957E-4
34-35	0.0	0.0	0.0	0.018880627686770995	7.285129654059548E-4
36-37	0.0	0.0	0.0	0.03208492518475392	7.285129654059548E-4
38-39	0.0	0.0	0.0	0.05269577116436406	7.285129654059548E-4
40-41	0.0	0.0	0.0	0.07667598960897673	7.285129654059548E-4
42-43	0.0	0.0	0.0	0.09874386151939879	7.588676722978696E-4
44-45	0.0	0.0	0.0	0.1248489094464455	8.195770860816991E-4
46-47	0.0	0.0	0.0	0.1549000692694411	8.802864998655286E-4
48-49	0.0	0.0	0.0	0.1862564814887891	9.71350620541273E-4
50-51	0.0	0.0	0.0	0.2203144626215175	9.71350620541273E-4
52-53	0.0	0.0	0.0	0.2540385419784348	0.0010017053274331876
54-55	0.0	0.0	0.0	0.29353001564481596	0.0010320600343251024
56-57	0.0	0.0	0.0	0.33611766941417237	0.001092769448108932
58-59	0.0	0.0	0.0	0.3830460462690726	0.001092769448108932
60-61	0.0	0.0	0.0	0.43565075331276093	0.001092769448108932
62-63	0.0	0.0	0.0	0.49028922571820754	0.0011534788618927617
64-65	0.0	0.0	0.0	0.5436224457273018	0.0011534788618927617
66-67	0.0	0.0	0.0	0.6063049154591058	0.0011534788618927617
68-69	0.0	0.0	0.0	0.6800668532064588	0.0011534788618927617
70-71	0.0	0.0	0.0	0.7587766081771938	0.0011838335687846765
72-73	0.0	0.0	0.0	0.8429198556815816	0.0013052523963523355
74-75	0.0	0.0	0.0	0.9249382737035352	0.00139631651702808
76-77	0.0	0.0	0.0	1.0193717668442823	0.0014873806377038242
78-79	0.0	0.0	0.0	1.1276470063277422	0.001517735344595739
80-81	0.0	0.0	0.0	1.2511906633778354	0.0015480900514876538
82-83	0.0	0.0	0.0	1.3811998729959063	0.0016998635859472278
84-85	0.0	0.0	0.0	1.5093574454935705	0.0016998635859472278
86-87	0.0	0.0	0.0	1.6470463959552961	0.0016998635859472278
88-89	0.0	0.0	0.0	1.8047694529656853	0.0017302182928391426
90-91	0.0	0.0	0.0	1.9793090175941952	0.0017605729997310572
92-93	0.0	0.0	0.0	2.165292306720957	0.0018212824135148869
94-95	0.0	0.0	0.0	2.359441012001644	0.0018819918272987165
96-97	0.0	0.0	0.0	2.561360522246661	0.0020034106548663753
98-99	0.0	0.0	0.0	2.778002065334257	0.0021248294824340345
100-101	0.0	0.0	0.0	3.025939311227417	0.0021248294824340345
102-103	0.0	0.0	0.0	3.2950641425311336	0.0022462483100016938
104-105	0.0	0.0	0.0	3.582341088556215	0.0022462483100016938
106-107	0.0	0.0	0.0	3.8757496853734628	0.0022766030168936083
108-109	0.0	0.0	0.0	4.183698186791938	0.0023373124306774384
110-111	0.0	0.0	0.0	4.523792322808951	0.0023980218444612676
112-113	0.0	0.0	0.0	4.892389528597473	0.0024890859651370122
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2450	0.0	81.77593	1
GTATCAA	4875	0.0	59.44481	1
TCAACGC	6495	0.0	44.280525	4
CAACGCA	6640	0.0	43.288563	5
AACGCAG	6940	0.0	41.577435	6
TATCAAC	6955	0.0	41.43871	2
ATCAACG	6950	0.0	41.381584	3
ACGCAGA	8365	0.0	34.35132	7
GTACATG	8485	0.0	33.169773	1
CGCAGAG	8725	0.0	32.864773	8
TACATGG	8590	0.0	32.026302	2
ACATGGG	8735	0.0	30.81203	3
GCAGAGT	9410	0.0	29.903399	9
CATGGGG	7385	0.0	29.752308	4
CAGAGTA	9190	0.0	26.087866	10-11
GTATAGG	460	0.0	25.925343	1
TATACCG	230	8.267307E-9	25.87416	5
AGAGTAC	8800	0.0	25.621561	10-11
GAGTACT	5300	0.0	24.637377	12-13
GTACTTT	5515	0.0	23.570107	14-15
>>END_MODULE