FastQCFastQC Report
Mon 15 Aug 2016
SRR2921496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045604
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.073.690871
GTATCAA44750.057.1830141
TCAACGC58500.042.916144
CAACGCA60350.041.501995
AACGCAG64250.038.981856
TATCAAC64650.038.7406652
GTACATG106550.038.0910151
ATCAACG66300.037.8671883
TACATGG112800.035.652592
ACATGGG115250.034.430953
ACGCAGA75500.033.014097
CATGGGG98300.031.6528134
CGCAGAG80200.031.0036568
GCAGAGT87100.027.454849
AGAGTAC78050.024.99777210-11
CAGAGTA83700.024.94491810-11
ATGGGGG52450.024.840645
GTATAGG5900.024.207551
TATATAG4250.022.3972745
TATACCG2153.4281165E-622.136845
Produced by FastQC (version 0.11.5)