##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921496_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2045604
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.86807172844793	31.0	30.0	34.0	25.0	34.0
2	29.900394211196303	31.0	30.0	34.0	25.0	34.0
3	29.970085607967132	31.0	30.0	34.0	25.0	34.0
4	33.34122635661643	37.0	35.0	37.0	27.0	37.0
5	33.332490061615054	37.0	35.0	37.0	27.0	37.0
6	33.346707378358666	37.0	35.0	37.0	27.0	37.0
7	33.15503538319245	37.0	35.0	37.0	26.0	37.0
8	33.12091147651256	37.0	35.0	37.0	26.0	37.0
9	34.575499461283805	39.0	35.0	39.0	26.0	39.0
10-11	34.47338829998377	39.0	35.0	39.0	25.0	39.0
12-13	34.35636198403992	39.0	35.0	39.0	25.0	39.0
14-15	35.51901736602001	40.0	36.0	41.0	25.0	41.0
16-17	35.46411744404098	40.0	36.0	41.0	25.0	41.0
18-19	35.48588803111453	40.0	36.0	41.0	25.0	41.0
20-21	35.505538706416296	40.0	36.0	41.0	25.0	41.0
22-23	35.48183128308314	40.0	36.0	41.0	25.0	41.0
24-25	35.44795840250606	40.0	35.0	41.0	25.0	41.0
26-27	35.37355103920407	40.0	35.0	41.0	24.5	41.0
28-29	35.32871587071594	40.0	35.0	41.0	24.5	41.0
30-31	35.23191023287009	40.0	35.0	41.0	24.0	41.0
32-33	35.12356448266625	40.0	35.0	41.0	24.0	41.0
34-35	34.95786672298255	40.0	35.0	41.0	24.0	41.0
36-37	34.81571384295299	39.0	35.0	41.0	23.0	41.0
38-39	34.68526704093266	39.0	34.0	41.0	22.5	41.0
40-41	34.53123502887166	39.0	34.0	41.0	21.5	41.0
42-43	34.33420691394815	39.0	34.0	41.0	19.5	41.0
44-45	34.134889255202864	39.0	33.0	41.0	18.0	41.0
46-47	33.87280138286785	39.0	33.0	41.0	15.0	41.0
48-49	33.717527439328435	38.0	33.0	41.0	14.5	41.0
50-51	33.50999362535467	38.0	33.0	40.0	12.5	41.0
52-53	33.30950223014816	38.0	33.0	40.0	9.5	41.0
54-55	33.09456864573984	37.5	33.0	40.0	8.5	41.0
56-57	32.83635591248355	37.0	32.0	40.0	7.5	41.0
58-59	32.58149524541407	37.0	32.0	40.0	7.0	41.0
60-61	32.26136656948266	36.0	32.0	40.0	4.0	41.0
62-63	31.93876478536413	35.5	31.0	39.0	2.0	41.0
64-65	31.62275176427109	35.0	31.0	39.0	2.0	41.0
66-67	31.560953390783357	35.0	31.0	39.0	2.0	41.0
68-69	31.3070249667091	35.0	31.0	37.5	2.0	40.0
70-71	30.998396072749173	35.0	31.0	37.0	2.0	39.5
72-73	30.67321314389295	35.0	31.0	37.0	2.0	39.0
74-75	30.379722810475535	35.0	31.0	36.0	2.0	39.0
76-77	30.11161862217712	35.0	30.5	36.0	2.0	38.0
78-79	29.85208109683008	35.0	30.0	35.0	2.0	37.0
80-81	29.631576052843073	35.0	30.0	35.0	2.0	37.0
82-83	29.452749896851984	35.0	30.0	35.0	2.0	36.0
84-85	29.2705746077931	35.0	30.0	35.0	2.0	36.0
86-87	29.132293933723243	34.5	30.0	35.0	2.0	36.0
88-89	29.019276213773537	34.0	30.0	35.0	2.0	35.0
90-91	28.918943989159192	34.0	30.0	35.0	2.0	35.0
92-93	28.82869802757523	34.0	30.0	35.0	2.0	35.0
94-95	28.76840923267651	34.0	29.0	35.0	2.0	35.0
96-97	28.67431697435085	34.0	29.0	35.0	2.0	35.0
98-99	28.616109227396898	34.0	29.0	35.0	2.0	35.0
100-101	28.550374119331014	34.0	29.0	35.0	2.0	35.0
102-103	27.593808479060463	33.0	27.5	34.5	2.0	35.0
104-105	28.401391960516307	34.0	29.0	35.0	2.0	35.0
106-107	28.725198767698927	34.5	30.0	35.0	2.0	35.0
108-109	28.76498432736737	35.0	30.0	35.0	2.0	35.0
110-111	28.709591396966374	35.0	30.0	35.0	2.0	35.0
112-113	28.639629419965935	35.0	30.0	35.0	2.0	35.0
114-115	28.518779783379383	35.0	30.0	35.0	2.0	35.0
116-117	28.459952170605845	35.0	30.0	35.0	2.0	35.0
118-119	28.30438638172393	35.0	29.5	35.0	2.0	35.0
120-121	28.134752131888675	34.5	29.0	35.0	2.0	35.0
122-123	28.010857428906085	34.5	29.0	35.0	2.0	35.0
124-125	27.183390578039543	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81815.0
3	14214.0
4	9008.0
5	7744.0
6	8496.0
7	9806.0
8	10705.0
9	10988.0
10	11619.0
11	12330.0
12	12595.0
13	13489.0
14	14927.0
15	12544.0
16	8842.0
17	7930.0
18	7246.0
19	6701.0
20	6494.0
21	6981.0
22	8137.0
23	11039.0
24	16345.0
25	22100.0
26	25630.0
27	27414.0
28	32148.0
29	38248.0
30	43315.0
31	51598.0
32	65312.0
33	85082.0
34	118504.0
35	190551.0
36	370714.0
37	530350.0
38	134476.0
39	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.02545080752668	16.853311138127015	12.245719321272677	24.87551873307363
2	17.069663233413003	19.10917629294664	37.78424093538885	26.036919538251507
3	19.274915075716716	23.814739638063877	28.595486580569634	28.31485870564977
4	13.659371230092074	14.958016642729968	34.91755652997564	36.465055597202316
5	14.808231256024024	36.978351900327496	32.85362825358434	15.359788590064143
6	34.306610396870305	33.792347235963895	17.00834314337794	14.892699223787861
7	30.234403998820426	31.150140135749016	20.1067780123562	18.508677853074353
8	28.49752309415176	32.77276528321817	19.230814371433183	19.498897251196887
9	28.065177378626156	14.358504922239762	18.366467742850382	39.209849956283705
10-11	26.838980786003354	24.60976497752079	26.34264589847031	22.20860833800555
12-13	27.087779903138642	22.19006333618843	26.5320997818211	24.190056978851832
14-15	24.450289828176526	23.284253137716735	23.9875329994814	28.27792403462534
16-17	24.707883353355083	26.261730940438692	24.406371195673835	24.624014510532387
18-19	24.863512990384223	25.368621914659943	25.855047555558926	23.912817539396908
20-21	25.926753850371785	24.313233275312495	25.57540046972665	24.184612404589068
22-23	25.716194649015588	23.88053240832449	25.506830206194998	24.896442736464923
24-25	25.142315849100072	24.36762846703421	25.442197202623774	25.047858481241946
26-27	24.84455114057781	24.386329450586373	25.63889499563127	25.130224413204548
28-29	24.82958453964044	24.536329335962325	25.642590085127644	24.99149603926959
30-31	25.168931476619573	24.44383199583687	25.40244720769083	24.984789319852727
32-33	24.89192734700346	24.44564769733592	25.339845572763046	25.32257938289757
34-35	25.2030989585371	24.535777302212033	25.432141571662193	24.828982167588673
36-37	25.269751228743075	24.35361682584447	25.404009353560582	24.972622591851877
38-39	25.311684652612488	24.4330782919368	25.307013296001614	24.948223759449103
40-41	25.291940502900882	24.384689217526333	25.42736793196838	24.896002347604405
42-43	25.143377862604755	24.45988428030794	25.347689788267886	25.049048068819417
44-45	25.08481548474446	24.454373280089687	25.239650211940884	25.221161023224965
46-47	25.19661986988211	24.568849973095926	25.199750525852373	25.034779631169595
48-49	25.29305560616261	24.739554357397864	24.849648137637278	25.117741898802247
50-51	25.222204044021897	24.939455588170347	24.824639466372815	25.013700901434945
52-53	25.37952168931205	24.72736877761378	24.912485799501805	24.98062373357236
54-55	25.137700020834743	24.754311470551123	24.98774858631891	25.120239922295223
56-57	25.141780219629737	24.759844164024898	24.837679010365264	25.260696605980105
58-59	25.311166283926656	24.586238526052774	24.879755405046204	25.222839784974372
60-61	25.231010578367552	24.725271501001505	25.13465021751377	24.909067703117177
62-63	25.09581776309966	24.88902158534444	24.963308777968347	25.051851873587555
64-65	25.161980043266407	24.62514796871145	25.043678431865434	25.16919355615671
66-67	25.063371239934384	24.850279562099658	24.988819716616188	25.097529481349774
68-69	25.227091352924607	24.72843414546365	24.86799459362188	25.17647990798986
70-71	25.04289918001858	24.61555805371122	25.075668113309103	25.265874652961102
72-73	25.316507381993723	24.890389911666443	24.805327788687983	24.987774917651844
74-75	25.06064757748353	25.08258120393358	24.857327506210247	24.999443712372642
76-77	25.179128413281067	24.71660362992834	24.811763880327916	25.29250407646268
78-79	25.156592075260043	24.780912906492684	24.88001005397164	25.182484964275638
80-81	25.094188410070302	25.03952123682768	24.70829097605353	25.15799937704849
82-83	25.17418391474498	24.923774674221075	24.800831388992652	25.101210022041293
84-85	25.094555634357015	24.987208005771492	24.67570186060259	25.24253449926891
86-87	24.881676362359784	25.04395576456139	24.83530034157394	25.239067531504887
88-89	24.984542702358578	24.893644746437975	24.789227003098304	25.33258554810514
90-91	24.953223050390033	24.825127301262135	24.883185966181745	25.33846368216609
92-93	25.04142571949226	24.96634847867743	24.77135789816974	25.220867903660572
94-95	24.987129058991204	24.821041360579475	24.817398872050866	25.37443070837846
96-97	24.986976629803582	24.829889484083864	24.839936607783024	25.343197278329527
98-99	24.97600691509308	24.894310644324946	24.68733209118497	25.442350349397007
100-101	24.913011061541283	24.956719427977756	24.81144044490619	25.31882906557477
102-103	24.96766033920711	24.771886837098066	24.962673724844485	25.29777909885034
104-105	24.8102575911019	25.031135868251337	24.7510780527023	25.407528487944457
106-107	24.874463619505597	24.92834000595476	24.8137915726967	25.383404801842946
108-109	24.87208088844104	24.877972342434774	24.853990946717545	25.39595582240664
110-111	24.84816500014422	25.06188043180148	24.82770522474709	25.262249343307207
112-113	24.92040184205721	25.148031184633258	24.72821736059964	25.203349612709893
114-115	24.601832083311535	25.295451178749122	24.891428750224282	25.21128798771506
116-117	24.660778325795075	25.28631167302915	24.84203388537853	25.210876115797248
118-119	24.85371806055141	25.081569945799725	24.721206715737683	25.343505277911184
120-121	24.600180404234028	25.09139802487537	24.964815777446823	25.343605793443775
122-123	24.664806203976408	25.145512664724052	24.82981754132374	25.359863589975802
124-125	24.637055190490262	25.300308062797583	24.838837178435163	25.223799568276988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	217.0
1	235.0
2	278.0
3	276.0
4	243.0
5	217.5
6	221.0
7	290.0
8	397.0
9	474.5
10	495.0
11	496.5
12	548.0
13	626.5
14	723.0
15	848.5
16	946.0
17	1032.5
18	1149.0
19	1293.5
20	1417.5
21	1624.0
22	1885.0
23	2200.5
24	2705.0
25	3346.0
26	4147.0
27	5072.5
28	6085.5
29	7432.5
30	9209.5
31	10949.5
32	12901.0
33	14655.0
34	16320.0
35	18801.0
36	22109.5
37	25719.5
38	28982.0
39	32765.0
40	36765.5
41	40410.0
42	44425.5
43	50316.0
44	56078.0
45	60180.5
46	66278.0
47	73068.5
48	80247.5
49	86790.0
50	88386.5
51	89091.0
52	90473.5
53	91792.0
54	93384.0
55	94016.0
56	90521.5
57	83576.0
58	78203.0
59	72724.0
60	65568.5
61	57631.0
62	49235.0
63	39023.5
64	29189.5
65	22524.5
66	17167.5
67	12770.5
68	9814.0
69	7719.0
70	6432.5
71	5339.5
72	4291.5
73	3756.5
74	3013.5
75	2254.5
76	1545.5
77	871.5
78	522.0
79	333.0
80	183.5
81	89.5
82	60.0
83	42.5
84	31.0
85	19.0
86	16.0
87	17.5
88	15.5
89	12.0
90	9.5
91	11.5
92	9.0
93	4.5
94	7.0
95	8.5
96	7.0
97	10.0
98	18.0
99	16.0
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03509965760723972
2	0.02483374103687713
3	0.026740268399944467
4	0.0306510937600826
5	0.03260650644015166
6	0.04551223012860749
7	0.03915713891838303
8	0.034904116339232814
9	0.02933119020103598
10-11	0.03492855899773367
12-13	0.03531964153374749
14-15	0.0335108848046836
16-17	0.03639511850778547
18-19	0.04866533307521886
20-21	0.059811185351612534
22-23	0.05294279831286994
24-25	0.06333092817573685
26-27	0.04695434698015843
28-29	0.047345429516172245
30-31	0.04829869319770591
32-33	0.056022573283978715
34-35	0.03852162979736058
36-37	0.05059630309678707
38-39	0.06012893991212376
40-41	0.04724765888216879
42-43	0.05773355937903915
44-45	0.05700027962401325
46-47	0.06374645337025153
48-49	0.048078709271198145
50-51	0.05037631917027929
52-53	0.059713414717609076
54-55	0.046049968615626484
56-57	0.0435079321315367
58-59	0.04485227834908418
60-61	0.0330464742931672
62-63	0.04091701033044519
64-65	0.040599255769933965
66-67	0.03468413241272505
68-69	0.0299178140050567
70-71	0.048127594588199867
72-73	0.030699979077084324
74-75	0.039034925625878715
76-77	0.03091996300359209
78-79	0.03133548819810677
80-81	0.024491543817865044
82-83	0.017207631584607773
84-85	0.018551977802155256
86-87	0.017892026022631947
88-89	0.022169491260283024
90-91	0.012661297103447197
92-93	0.01842976450965094
94-95	0.014616709783516263
96-97	0.011854689372918708
98-99	0.010901425691385039
100-101	0.011194737593395399
102-103	0.006257320576221009
104-105	0.006526189819730505
106-107	0.00884824237731252
108-109	0.0131257076149636
110-111	0.006208435259219282
112-113	0.008481602499799571
114-115	0.008457159841298707
116-117	0.007308354891758131
118-119	0.2455998326166746
120-121	0.3619224444222831
122-123	0.02898899298202389
124-125	0.0038863827016372672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2045604.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.992691261709034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54929573396801	41.870951821271035
2	11.416682224671831	12.328347971794953
3	3.973886751661971	6.436825214744417
4	2.00827231268022	4.337281077919261
5	1.192526469574711	3.2193856746581617
6	0.7446091510408652	2.4122071201675626
7	0.5391647689610751	2.0377669836794206
8	0.4020010113943237	1.7364093196086794
9	0.3064436584207596	1.4891146054398625
>10	1.733973362784013	16.896326413838658
>50	0.09899820244537225	3.650091364284449
>100	0.03286541835468345	3.0006343343419153
>500	0.0010064481759294531	0.33592522755482734
>1k	2.7448586616257644E-4	0.24873287069684513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.888531700172663E-5
2	0.0	0.0	0.0	0.0	4.888531700172663E-5
3	0.0	0.0	0.0	9.777063400345326E-5	4.888531700172663E-5
4	0.0	0.0	0.0	9.777063400345326E-5	4.888531700172663E-5
5	0.0	0.0	0.0	9.777063400345326E-5	4.888531700172663E-5
6	0.0	0.0	0.0	9.777063400345326E-5	9.777063400345326E-5
7	0.0	0.0	0.0	1.466559510051799E-4	9.777063400345326E-5
8	0.0	0.0	0.0	1.466559510051799E-4	9.777063400345326E-5
9	0.0	0.0	0.0	1.466559510051799E-4	9.777063400345326E-5
10-11	0.0	0.0	0.0	1.466559510051799E-4	1.466559510051799E-4
12-13	0.0	0.0	0.0	1.466559510051799E-4	1.9554126800690652E-4
14-15	0.0	0.0	0.0	1.466559510051799E-4	2.933119020103597E-4
16-17	0.0	0.0	0.0	1.466559510051799E-4	3.9108253601381304E-4
18-19	0.0	0.0	0.0	1.466559510051799E-4	4.3996785301553964E-4
20-21	0.0	0.0	0.0	1.466559510051799E-4	4.3996785301553964E-4
22-23	0.0	0.0	0.0	1.466559510051799E-4	5.377384870189929E-4
24-25	0.0	0.0	0.0	1.9554126800690652E-4	5.866238040207196E-4
26-27	0.0	0.0	0.0	3.666398775129497E-4	6.110664625215828E-4
28-29	4.888531700172663E-5	0.0	0.0	0.0011243622910397126	6.355091210224462E-4
30-31	4.888531700172663E-5	0.0	0.0	0.00391082536013813	6.355091210224462E-4
32-33	4.888531700172663E-5	0.0	0.0	0.009899276692849642	6.843944380241728E-4
34-35	1.222132925043166E-4	0.0	0.0	0.016963204999599142	7.821650720276261E-4
36-37	1.466559510051799E-4	0.0	0.0	0.029355632859536844	7.821650720276261E-4
38-39	1.466559510051799E-4	0.0	0.0	0.04790761066169209	7.821650720276261E-4
40-41	1.466559510051799E-4	0.0	0.0	0.06785281999839657	7.821650720276261E-4
42-43	1.466559510051799E-4	0.0	0.0	0.089411244796158	7.821650720276261E-4
44-45	1.466559510051799E-4	0.0	0.0	0.11292508227398851	7.821650720276261E-4
46-47	1.466559510051799E-4	0.0	0.0	0.14054528637996405	8.55493047530216E-4
48-49	1.466559510051799E-4	0.0	0.0	0.1690209835334698	8.799357060310793E-4
50-51	1.466559510051799E-4	0.0	0.0	0.19991650387856105	9.288210230328059E-4
52-53	1.466559510051799E-4	0.0	0.0	0.23110533612566264	0.0011488049495405757
54-55	1.466559510051799E-4	0.0	0.0	0.2661072230988989	0.0012710182420448924
56-57	1.466559510051799E-4	0.0	0.0	0.3077819558428708	0.0012954609005457556
58-59	1.466559510051799E-4	0.0	0.0	0.3503366242928739	0.001319903559046619
60-61	1.466559510051799E-4	0.0	0.0	0.4006396154876506	0.001319903559046619
62-63	1.710986095060432E-4	0.0	0.0	0.45275136341149114	0.0014176741930500723
64-65	1.9554126800690652E-4	0.0	0.0	0.5063541135038844	0.0014176741930500723
66-67	1.9554126800690652E-4	0.0	0.0	0.5673629891220393	0.0014176741930500723
68-69	1.9554126800690652E-4	0.0	0.0	0.6378800588970299	0.0014176741930500723
70-71	1.9554126800690652E-4	0.0	0.0	0.7163165500263002	0.0014421168515509355
72-73	1.9554126800690652E-4	0.0	0.0	0.7981505706871908	0.0015154448270535256
74-75	1.9554126800690652E-4	0.0	0.0	0.8806445431276044	0.0015154448270535256
76-77	1.9554126800690652E-4	0.0	0.0	0.973160005553372	0.0015154448270535256
78-79	1.9554126800690652E-4	0.0	0.0	1.0800233085191464	0.0015154448270535256
80-81	1.9554126800690652E-4	0.0	0.0	1.2000367617583851	0.0015154448270535256
82-83	1.9554126800690652E-4	0.0	0.0	1.32955840915446	0.0015643301440552522
84-85	1.9554126800690652E-4	0.0	0.0	1.459935549598065	0.0015643301440552522
86-87	1.9554126800690652E-4	0.0	0.0	1.5991609324189824	0.001613215461056979
88-89	1.9554126800690652E-4	0.0	0.0	1.7536874194614402	0.0016621007780587055
90-91	1.9554126800690652E-4	0.0	0.0	1.9302611844716768	0.0016621007780587055
92-93	1.9554126800690652E-4	0.0	0.0	2.1181274577093125	0.0016865434365595689
94-95	1.9554126800690652E-4	0.0	0.0	2.3121288382306644	0.0017598714120621586
96-97	1.9554126800690652E-4	0.0	0.0	2.51500290378783	0.0017598714120621586
98-99	1.9554126800690652E-4	0.0	0.0	2.734229107882073	0.0017598714120621586
100-101	1.9554126800690652E-4	0.0	0.0	2.989899315801103	0.0018087567290638853
102-103	1.9554126800690652E-4	0.0	0.0	3.260528430722662	0.0018576420460656119
104-105	1.9554126800690652E-4	0.0	0.0	3.5493917688858643	0.0019065273630673387
106-107	1.9554126800690652E-4	0.0	0.0	3.84456131294229	0.0019065273630673387
108-109	1.9554126800690652E-4	0.0	0.0	4.156987373900325	0.0019065273630673387
110-111	1.9554126800690652E-4	0.0	0.0	4.499477904814421	0.0019554126800690654
112-113	1.9554126800690652E-4	0.0	0.0	4.865066748011834	0.0020042979970707918
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2455	0.0	73.69087	1
GTATCAA	4475	0.0	57.183014	1
TCAACGC	5850	0.0	42.91614	4
CAACGCA	6035	0.0	41.50199	5
AACGCAG	6425	0.0	38.98185	6
TATCAAC	6465	0.0	38.740665	2
GTACATG	10655	0.0	38.091015	1
ATCAACG	6630	0.0	37.867188	3
TACATGG	11280	0.0	35.65259	2
ACATGGG	11525	0.0	34.43095	3
ACGCAGA	7550	0.0	33.01409	7
CATGGGG	9830	0.0	31.652813	4
CGCAGAG	8020	0.0	31.003656	8
GCAGAGT	8710	0.0	27.45484	9
AGAGTAC	7805	0.0	24.997772	10-11
CAGAGTA	8370	0.0	24.944918	10-11
ATGGGGG	5245	0.0	24.84064	5
GTATAGG	590	0.0	24.20755	1
TATATAG	425	0.0	22.397274	5
TATACCG	215	3.4281165E-6	22.13684	5
>>END_MODULE