FastQCFastQC Report
Mon 15 Aug 2016
SRR2921496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045604
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.18292885622046104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.1630325322007583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.076.728831
GTATCAA60750.061.0474241
TCAACGC82500.044.5295564
CAACGCA83500.044.11195
TATCAAC87300.042.356082
AACGCAG88150.042.0457156
ATCAACG90350.040.8593373
ACGCAGA102100.036.3000877
GTACATG106300.035.0566181
CGCAGAG108900.034.141848
ACATGGG111150.032.6238823
TACATGG112500.032.4448932
GCAGAGT116100.031.255939
CATGGGG97750.029.3594194
GTGGTAT12550.028.9807321
AGAGTAC106750.028.03110310-11
CAGAGTA111650.026.74761810-11
GAGTACT60950.025.37603812-13
TACCGTA2001.8296305E-623.7960787
ATGGGGG52650.023.7340875
Produced by FastQC (version 0.11.5)