FastQCFastQC Report
Mon 15 Aug 2016
SRR2921495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710715
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.12702291147268832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.073.518271
GTATCAA42450.060.558991
TCAACGC51750.048.7417454
CAACGCA53100.047.0530325
ATCAACG55600.045.5779723
AACGCAG55200.045.1565256
TATCAAC59800.042.874222
GTACATG92350.038.533361
ACGCAGA65750.037.5478637
TACATGG95950.036.5786132
ACATGGG97300.035.5819973
CGCAGAG70300.035.116648
CATGGGG76850.033.3640674
GCAGAGT76550.031.8609589
ATGGGGG40500.026.8799785
AGAGTAC69400.026.4877210-11
CAGAGTA72700.026.3082610-11
ATGGGGA30000.025.1834555
GACCGTA1701.4013822E-524.4952567
GTGGTAT9850.024.1655311
Produced by FastQC (version 0.11.5)