##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921495_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1710715
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.20420350555177	31.0	30.0	34.0	23.0	34.0
2	29.29156580727941	31.0	30.0	34.0	22.0	34.0
3	29.43656658180936	31.0	30.0	34.0	23.0	34.0
4	32.75569747152507	37.0	33.0	37.0	24.0	37.0
5	32.77655775509071	37.0	35.0	37.0	25.0	37.0
6	32.78961369953499	37.0	35.0	37.0	25.0	37.0
7	32.60964450536764	37.0	33.0	37.0	23.0	37.0
8	32.55193822466045	37.0	33.0	37.0	22.0	37.0
9	33.92365999012109	39.0	35.0	39.0	21.0	39.0
10-11	33.79505236114724	39.0	34.0	39.0	18.5	39.0
12-13	33.625347880856836	39.0	34.0	39.0	17.0	39.0
14-15	34.69959402939706	40.0	34.0	41.0	17.0	41.0
16-17	34.60772250199478	39.5	34.0	41.0	17.0	41.0
18-19	34.62821919489804	40.0	34.0	41.0	17.5	41.0
20-21	34.64869250576513	40.0	34.0	41.0	18.0	41.0
22-23	34.6137413303794	39.5	34.0	41.0	18.0	41.0
24-25	34.57490581423557	39.0	34.0	41.0	18.0	41.0
26-27	34.488802927430925	39.0	34.0	41.0	18.0	41.0
28-29	34.43031276396127	39.0	34.0	41.0	18.0	41.0
30-31	34.31678567148823	39.0	34.0	41.0	17.0	41.0
32-33	34.192491151360684	39.0	33.5	41.0	16.5	41.0
34-35	34.011555402273316	39.0	33.0	41.0	16.0	41.0
36-37	33.86032331510509	39.0	33.0	41.0	15.0	41.0
38-39	33.719009595403094	39.0	33.0	41.0	11.0	41.0
40-41	33.54867000055532	38.5	33.0	41.0	9.0	41.0
42-43	33.336996811274815	38.0	33.0	41.0	8.5	41.0
44-45	33.13264629117065	38.0	32.5	40.5	7.0	41.0
46-47	32.848082234621195	38.0	32.0	40.0	2.0	41.0
48-49	32.683106478869945	38.0	32.0	40.0	2.0	41.0
50-51	32.481976249696764	38.0	32.0	40.0	2.0	41.0
52-53	32.27281633702867	37.5	31.0	40.0	2.0	41.0
54-55	32.06244289668355	37.0	31.0	40.0	2.0	41.0
56-57	31.820966087279295	36.5	31.0	40.0	2.0	41.0
58-59	31.56729028505625	36.0	31.0	40.0	2.0	41.0
60-61	31.26270565231497	35.5	30.5	39.5	2.0	41.0
62-63	30.95449066618344	35.0	30.0	39.0	2.0	41.0
64-65	30.648602192650443	35.0	30.0	39.0	2.0	40.5
66-67	30.610230225373602	35.0	30.0	38.5	2.0	40.0
68-69	30.369021724834354	35.0	30.0	37.5	2.0	40.0
70-71	30.073312036195393	35.0	30.0	37.0	2.0	39.5
72-73	29.76454961814212	35.0	30.0	36.5	2.0	39.0
74-75	29.47211546049459	35.0	29.0	36.0	2.0	39.0
76-77	29.205783838921153	35.0	29.0	36.0	2.0	38.5
78-79	28.940111590767604	34.5	29.0	35.0	2.0	37.0
80-81	28.70936304410729	34.0	29.0	35.0	2.0	37.0
82-83	28.510416404836576	34.0	29.0	35.0	2.0	36.0
84-85	28.314320620325418	34.0	29.0	35.0	2.0	36.0
86-87	28.16560210204505	34.0	29.0	35.0	2.0	36.0
88-89	28.03779764601351	34.0	28.5	35.0	2.0	35.0
90-91	27.92391163928533	34.0	28.0	35.0	2.0	35.0
92-93	27.82497581420634	34.0	28.0	35.0	2.0	35.0
94-95	27.754759267323898	34.0	28.0	35.0	2.0	35.0
96-97	27.64741555431501	34.0	27.0	35.0	2.0	35.0
98-99	27.584870945774135	34.0	27.0	35.0	2.0	35.0
100-101	27.513085171989488	34.0	27.0	35.0	2.0	35.0
102-103	26.554799601336285	32.5	25.0	34.5	2.0	35.0
104-105	27.360384108399117	34.0	27.0	35.0	2.0	35.0
106-107	27.685735204285926	34.0	28.0	35.0	2.0	35.0
108-109	27.7244844991714	34.0	29.0	35.0	2.0	35.0
110-111	27.661714546256974	34.0	28.0	35.0	2.0	35.0
112-113	27.58525879529904	34.0	28.0	35.0	2.0	35.0
114-115	27.457907073942767	34.0	27.0	35.0	2.0	35.0
116-117	27.388912238450004	34.0	27.0	35.0	2.0	35.0
118-119	27.227809132438775	34.0	27.0	35.0	2.0	35.0
120-121	27.04458019015441	34.0	27.0	35.0	2.0	35.0
122-123	26.913215819116566	34.0	27.0	35.0	2.0	35.0
124-125	26.058969202935614	33.0	23.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86681.0
3	14791.0
4	9591.0
5	8083.0
6	8655.0
7	9754.0
8	10582.0
9	10874.0
10	11642.0
11	12169.0
12	12387.0
13	12850.0
14	14370.0
15	11803.0
16	8790.0
17	7631.0
18	6907.0
19	6272.0
20	6264.0
21	6398.0
22	7617.0
23	10193.0
24	14539.0
25	19994.0
26	23844.0
27	24957.0
28	29416.0
29	34389.0
30	38289.0
31	45682.0
32	56970.0
33	73763.0
34	102454.0
35	159867.0
36	293259.0
37	397984.0
38	100928.0
39	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.277161836596264	17.01219797441115	12.224522255748134	24.48611793324445
2	16.88367578440201	19.206183999719332	38.043351147805545	25.866789068073114
3	19.384329143648348	24.032353505255426	28.532503192549314	28.05081415854691
4	13.461862325450847	14.968949547400182	35.486457090730475	36.082731036418494
5	14.490132338760562	37.087833851145064	33.11679388286286	15.305239927231518
6	33.776731842056165	34.376942731705206	17.048784752783643	14.797540673454982
7	30.13266778202649	31.280638526929742	20.1534331231459	18.433260567897868
8	28.16406083249078	32.89350805491929	19.31379537598687	19.628635736603055
9	27.857824910111432	14.746344375685968	18.761201352143907	38.63462936205869
10-11	26.51171888591275	24.86290858067291	26.48771418204337	22.137658351370973
12-13	26.860539198415506	22.59678008368636	26.6840850009254	23.85859571697273
14-15	24.24661943964374	23.7520017004769	24.161361691378062	27.840017168501298
16-17	24.247911396975944	26.748621169676174	24.616642335083473	24.38682509826441
18-19	24.34876795201411	25.713986604563242	26.403380207224554	23.533865236198093
20-21	25.57670581415855	24.66593220641355	26.066076271367365	23.69128570806053
22-23	25.323475370543612	24.26191759445744	25.968305920480255	24.44630111451869
24-25	24.689209603097886	24.779437893834793	26.04161030445102	24.489742198616305
26-27	24.33150920631467	24.8297959071479	26.177860279707275	24.660834606830154
28-29	24.38385406843028	24.88933922276467	26.186008360419162	24.540798348385888
30-31	24.727675311713842	24.785633222339936	26.008422319585534	24.478269146360685
32-33	24.373190870376686	24.83887816270513	25.96578058185761	24.822150385060574
34-35	24.736566946410473	24.85171200052397	26.144301489400394	24.267419563665158
36-37	24.817910788202767	24.63134007775729	25.922479577379328	24.628269556660616
38-39	24.777742617206076	24.917803227272543	25.870004802245184	24.434449353276204
40-41	24.833906084417816	24.794663472672873	26.024197076279215	24.347233366630093
42-43	24.62552367026138	24.81684518369585	25.854227746298662	24.703403399744104
44-45	24.542268946933437	24.912396040669528	25.804549732318797	24.740785280078235
46-47	24.734972307614917	24.994304174900876	25.675141722949803	24.595581794534407
48-49	24.910971149915284	25.120669255994276	25.32027869290617	24.64808090118426
50-51	24.79861856399669	25.298906606855127	25.262499159265765	24.639975669882414
52-53	24.936456719648888	25.176270273384528	25.34931622429352	24.537956782673064
54-55	24.820346788085676	25.1358054005181	25.46676207233032	24.5770857390659
56-57	24.687015873925603	25.254150654624713	25.283684355501546	24.77514911594814
58-59	24.81224114074107	25.10523970323515	25.334199657758965	24.748319498264816
60-61	24.817783095148087	25.199832643176485	25.486976487301906	24.495407774373533
62-63	24.650173644869707	25.337267548775394	25.387064166289708	24.625494640065195
64-65	24.711962247412316	25.019715194749864	25.51296787585553	24.755354681982286
66-67	24.641469459884842	25.273899526364556	25.391380062985707	24.693250950764895
68-69	24.765176510488008	25.21943026048618	25.306556229141297	24.708836999884515
70-71	24.61727547393157	24.99397606568077	25.545955600679825	24.84279285970783
72-73	24.927719279780256	25.2627439629628	25.281513999318783	24.52802275793816
74-75	24.64829400699947	25.450902682565037	25.212421267653284	24.68838204278221
76-77	24.66685417601046	25.166609458774353	25.371388374147802	24.79514799106738
78-79	24.71261460841995	25.21956837443531	25.320117079742815	24.747699937401926
80-81	24.66654056723112	25.442812200617382	25.142395400079693	24.7482518320718
82-83	24.86844624646834	25.31915360093778	25.155802148299617	24.65659800429426
84-85	24.620314202366764	25.319451155859074	25.144666917485996	24.915567724288167
86-87	24.445933356193194	25.401955015181933	25.280026005285833	24.872085623339043
88-89	24.630970446939934	25.249125826850428	25.20901661988721	24.910887106322434
90-91	24.526653362615107	25.172368535284722	25.347345946946128	24.953632155154043
92-93	24.68722566379313	25.40782639632105	25.1524150940859	24.75253284579992
94-95	24.538049864773694	25.13978646754744	25.35420172046682	24.967961947212046
96-97	24.577815479378057	25.17258030947531	25.259922783173188	24.989681427973444
98-99	24.497150144794684	25.175158160460047	25.160922769055478	25.166768925689798
100-101	24.470235126917323	25.304307512965334	25.324418321180133	24.901039038937213
102-103	24.53875547207688	25.12465535086327	25.371145432070836	24.965443744989013
104-105	24.389034807989884	25.352110617655775	25.23121828256888	25.02763629178546
106-107	24.373557866282233	25.3066370157836	25.289566712440802	25.030238405493364
108-109	24.39871955231686	25.246427131787254	25.208309522006928	25.146543793888966
110-111	24.464067901539494	25.350981778557102	25.226142139472564	24.95880818043084
112-113	24.41758670249474	25.350050114683558	25.326198506760733	24.906164676060964
114-115	24.168700097160368	25.5985710059898	25.317203465035714	24.915525431814117
116-117	24.23418593965385	25.642933056159308	25.18929052880922	24.933590475377617
118-119	24.34722383358119	25.267925050127914	25.279819809051034	25.105031307239866
120-121	24.065522052597263	25.344655699200185	25.46092191128278	25.128900336919774
122-123	24.142628936024906	25.459967910711324	25.295077615382276	25.102325537881498
124-125	24.108402037704373	25.536572198704526	25.289297843130438	25.065727920460667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	152.0
2	172.0
3	167.0
4	149.0
5	150.0
6	181.0
7	214.5
8	280.5
9	367.5
10	416.5
11	463.0
12	539.5
13	631.5
14	726.5
15	829.0
16	931.0
17	1018.0
18	1125.5
19	1222.5
20	1332.0
21	1545.5
22	1833.5
23	2113.5
24	2462.0
25	3074.5
26	4152.0
27	5393.0
28	6197.0
29	7496.5
30	9594.0
31	11742.5
32	14427.0
33	16968.0
34	19397.5
35	22076.5
36	25515.0
37	29136.5
38	30988.0
39	33009.5
40	35586.0
41	37700.5
42	40097.0
43	44590.0
44	49477.5
45	53014.0
46	56706.5
47	59849.5
48	64289.5
49	69195.0
50	69961.5
51	69591.0
52	69811.5
53	70375.0
54	71741.0
55	71429.0
56	67882.0
57	62305.0
58	58537.5
59	54950.0
60	49450.5
61	43909.0
62	38513.0
63	30976.5
64	23437.0
65	18426.0
66	14182.0
67	10894.5
68	8918.5
69	7301.5
70	6140.5
71	5064.0
72	4115.5
73	3792.0
74	3145.0
75	2405.0
76	1691.5
77	1050.5
78	687.0
79	438.5
80	251.0
81	133.0
82	82.5
83	53.0
84	37.5
85	30.5
86	33.0
87	24.0
88	19.5
89	15.5
90	9.5
91	11.0
92	12.0
93	10.5
94	9.5
95	9.0
96	7.0
97	10.0
98	10.5
99	11.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034780778797169606
2	0.028935269755628497
3	0.028233808670643564
4	0.034780778797169606
5	0.03706052732337064
6	0.047699353778975453
7	0.038229629131678856
8	0.03752816804669393
9	0.03168265900515282
10-11	0.037031299778162934
12-13	0.037382030320655395
14-15	0.03545301233694683
16-17	0.03860958721937903
18-19	0.05138202447514636
20-21	0.06251771919928217
22-23	0.056321479615248596
24-25	0.0679540426079154
26-27	0.050037557395591896
28-29	0.051002066387446185
30-31	0.04989141966955337
32-33	0.05751980896876452
34-35	0.04080165310995695
36-37	0.05304799455198557
38-39	0.06418368927612139
40-41	0.049043820858529916
42-43	0.06006260540183491
44-45	0.060325653308704257
46-47	0.06602502462420683
48-49	0.05120665920390012
50-51	0.052609581373869986
52-53	0.061582437752635596
54-55	0.04813776695709104
56-57	0.046997892693990524
58-59	0.047290168146067575
60-61	0.03486846143279272
62-63	0.04439664117050473
64-65	0.043227539362196504
66-67	0.03767430577273245
68-69	0.03200416200243758
70-71	0.05079747357099225
72-73	0.03171188655036052
74-75	0.042584533367626985
76-77	0.03378704226010761
78-79	0.03498537161362354
80-81	0.024814185881342012
82-83	0.017098113946507745
84-85	0.01873485647813926
86-87	0.01715656903692316
88-89	0.022475982264725566
90-91	0.012187886351613214
92-93	0.019670137924785835
94-95	0.014555317513437364
96-97	0.012100203715990097
98-99	0.011077239633720403
100-101	0.011223377359758932
102-103	0.005757826405917993
104-105	0.005962419222371932
106-107	0.008300622838988376
108-109	0.012830892346182737
110-111	0.007043838395057038
112-113	0.008096030022534438
114-115	0.008242167748572966
116-117	0.006839245578603099
118-119	0.2385552239852927
120-121	0.3511105005801668
122-123	0.028467629032305205
124-125	0.003770353331794016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1710715.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.29480619730293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03465707397218	48.64229092663387
2	9.282650930873253	11.008259758866863
3	3.162038139842734	5.624773160713656
4	1.5130241123108608	3.588578860452752
5	0.9278485008934356	2.7508298520467167
6	0.5857870414177743	2.0840477456255035
7	0.42743659167981507	1.7741338905703237
8	0.31630146964043754	1.5004027473801471
9	0.2523738332255777	1.3468011777342972
>10	1.386528793063063	14.940364689108618
>50	0.08126848549315925	3.2818153451556324
>100	0.029290816208632827	2.9792997958514444
>500	4.963821119578969E-4	0.19319743172649873
>1k	2.978292671747381E-4	0.2852046181337253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2173	0.12702291147268832	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.84550904154111E-5
2	0.0	0.0	0.0	0.0	5.84550904154111E-5
3	0.0	0.0	0.0	0.0	5.84550904154111E-5
4	0.0	0.0	0.0	0.0	5.84550904154111E-5
5	0.0	0.0	0.0	0.0	5.84550904154111E-5
6	0.0	0.0	0.0	0.0	5.84550904154111E-5
7	0.0	0.0	0.0	0.0	5.84550904154111E-5
8	0.0	0.0	0.0	0.0	1.169101808308222E-4
9	0.0	0.0	0.0	0.0	1.169101808308222E-4
10-11	0.0	0.0	0.0	0.0	1.169101808308222E-4
12-13	0.0	0.0	0.0	0.0	1.169101808308222E-4
14-15	0.0	0.0	0.0	0.0	1.4613772603852776E-4
16-17	0.0	0.0	0.0	0.0	1.753652712462333E-4
18-19	0.0	0.0	0.0	0.0	2.922754520770555E-4
20-21	0.0	0.0	0.0	5.84550904154111E-5	3.799580877001721E-4
22-23	0.0	0.0	0.0	1.169101808308222E-4	4.091856329078777E-4
24-25	0.0	0.0	0.0	1.169101808308222E-4	4.091856329078777E-4
26-27	0.0	0.0	0.0	1.753652712462333E-4	4.091856329078777E-4
28-29	0.0	0.0	0.0	9.352814466465776E-4	4.091856329078777E-4
30-31	0.0	0.0	0.0	0.0036242156057554884	4.091856329078777E-4
32-33	0.0	0.0	0.0	0.010258868367904649	4.676407233232888E-4
34-35	0.0	0.0	0.0	0.018150305573985146	4.676407233232888E-4
36-37	0.0	0.0	0.0	0.031185790736621824	4.676407233232888E-4
38-39	0.0	0.0	0.0	0.0526388089190777	5.553233589464055E-4
40-41	0.0	0.0	0.0	0.07455946782485685	6.722335397772276E-4
42-43	0.0	0.0	0.0	0.09589557582648191	7.014610849849332E-4
44-45	0.0	0.0	0.0	0.12050516889136997	7.306886301926387E-4
46-47	0.0	0.0	0.0	0.14987885182511407	7.599161754003443E-4
48-49	0.0	0.0	0.0	0.1806846844740357	7.599161754003443E-4
50-51	0.0	5.84550904154111E-5	0.0	0.2131857147450043	7.599161754003443E-4
52-53	0.0	5.84550904154111E-5	0.0	0.24951555343818227	7.599161754003443E-4
54-55	0.0	5.84550904154111E-5	0.0	0.28610844003822966	7.599161754003443E-4
56-57	0.0	5.84550904154111E-5	0.0	0.3284006979537796	7.891437206080499E-4
58-59	0.0	5.84550904154111E-5	0.0	0.38065954878515706	8.183712658157554E-4
60-61	0.0	5.84550904154111E-5	0.0	0.4339121361535966	8.183712658157554E-4
62-63	0.0	5.84550904154111E-5	0.0	0.48760313670015165	9.352814466465776E-4
64-65	0.0	5.84550904154111E-5	0.0	0.5472857840142864	9.352814466465776E-4
66-67	0.0	5.84550904154111E-5	0.0	0.6116740661068618	9.352814466465776E-4
68-69	0.0	8.768263562311665E-5	0.0	0.68749031837565	9.352814466465776E-4
70-71	0.0	1.4613772603852776E-4	0.0	0.770964187488857	9.352814466465776E-4
72-73	0.0	1.753652712462333E-4	0.0	0.8557240685912031	9.645089918542832E-4
74-75	0.0	1.753652712462333E-4	0.0	0.9472939677269445	9.937365370619887E-4
76-77	0.0	1.753652712462333E-4	0.0	1.0498241963155754	9.937365370619887E-4
78-79	0.0	1.753652712462333E-4	0.0	1.1643961735297814	9.937365370619887E-4
80-81	0.0	1.753652712462333E-4	0.0	1.2932311928053473	0.0010229640822696942
82-83	0.0	1.753652712462333E-4	0.0	1.432734266081726	0.0010521916274773997
84-85	0.0	1.753652712462333E-4	0.0	1.5726465249910127	0.0010814191726851053
86-87	0.0	2.338203616616444E-4	0.0	1.725652724153351	0.0011106467178928108
88-89	0.0	2.338203616616444E-4	0.0	1.9003749894050148	0.0011106467178928108
90-91	0.0	2.338203616616444E-4	0.0	2.0924291889648483	0.0011106467178928108
92-93	0.0	2.338203616616444E-4	0.0	2.300353945572465	0.0011983293535159276
94-95	0.0	2.338203616616444E-4	0.0	2.5109676363391915	0.0012275568987236331
96-97	0.0	2.338203616616444E-4	0.0	2.7316356026573683	0.0013152395343467497
98-99	0.0	2.338203616616444E-4	0.0	2.977643850670626	0.0013444670795544553
100-101	0.0	2.338203616616444E-4	0.0	3.2567084523137986	0.0013444670795544553
102-103	0.0	2.338203616616444E-4	0.0	3.5550340062488495	0.0014321497151775719
104-105	0.0	2.338203616616444E-4	0.0	3.871656003483923	0.0014906048055929831
106-107	0.0	2.338203616616444E-4	0.0	4.19640325828674	0.0015490598960083942
108-109	0.0	2.338203616616444E-4	0.0	4.539680776751242	0.0016367425316315108
110-111	0.0	2.338203616616444E-4	0.0	4.919317361454128	0.0017536527124623331
112-113	0.0	2.338203616616444E-4	0.0	5.325726377567275	0.0018121078028777442
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1975	0.0	73.51827	1
GTATCAA	4245	0.0	60.55899	1
TCAACGC	5175	0.0	48.741745	4
CAACGCA	5310	0.0	47.053032	5
ATCAACG	5560	0.0	45.577972	3
AACGCAG	5520	0.0	45.156525	6
TATCAAC	5980	0.0	42.87422	2
GTACATG	9235	0.0	38.53336	1
ACGCAGA	6575	0.0	37.547863	7
TACATGG	9595	0.0	36.578613	2
ACATGGG	9730	0.0	35.581997	3
CGCAGAG	7030	0.0	35.11664	8
CATGGGG	7685	0.0	33.364067	4
GCAGAGT	7655	0.0	31.860958	9
ATGGGGG	4050	0.0	26.879978	5
AGAGTAC	6940	0.0	26.48772	10-11
CAGAGTA	7270	0.0	26.30826	10-11
ATGGGGA	3000	0.0	25.183455	5
GACCGTA	170	1.4013822E-5	24.495256	7
GTGGTAT	985	0.0	24.165531	1
>>END_MODULE