FastQCFastQC Report
Mon 15 Aug 2016
SRR2921495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710715
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.19103123547756348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.16864293584846102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.078.389191
GTATCAA56350.063.0345461
TCAACGC76800.045.742834
CAACGCA79100.044.3141485
ATCAACG80850.043.598743
TATCAAC81600.043.3439562
AACGCAG82000.042.80956
ACGCAGA96750.036.4038547
CGCAGAG102150.034.3609288
GTACATG89600.033.6564941
GCAGAGT106300.032.570799
TACATGG94600.031.2822152
ACATGGG95800.030.7660663
CATGGGG75350.027.578744
AGAGTAC99500.027.4730810-11
CAGAGTA103350.027.28429610-11
GTGGTAT10350.027.0634941
GAGTACT58150.025.6788312-13
ATGGGGG41700.025.2604355
TGGTATC12300.024.688652
Produced by FastQC (version 0.11.5)