##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1710715
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19368042017519	31.0	31.0	34.0	28.0	34.0
2	31.260566488281217	33.0	31.0	34.0	28.0	34.0
3	31.336555767617632	33.0	31.0	34.0	28.0	34.0
4	34.82523681618505	37.0	35.0	37.0	32.0	37.0
5	34.487606644005574	37.0	35.0	37.0	30.0	37.0
6	34.46594786390486	37.0	35.0	37.0	30.0	37.0
7	34.29932396687935	37.0	35.0	37.0	30.0	37.0
8	34.27098610814776	37.0	35.0	37.0	30.0	37.0
9	35.8237526414394	39.0	37.0	39.0	30.0	39.0
10-11	35.69420826964164	39.0	36.0	39.0	30.0	39.0
12-13	35.603034111468006	39.0	35.0	39.0	30.0	39.0
14-15	36.772697965470584	40.0	37.0	41.0	30.0	41.0
16-17	36.75765016382039	40.0	37.0	41.0	30.0	41.0
18-19	36.78997056786197	40.0	37.0	41.0	30.0	41.0
20-21	36.780047231713056	40.0	37.0	41.0	30.0	41.0
22-23	36.720579114580744	40.0	37.0	41.0	30.0	41.0
24-25	36.65664970494793	40.0	36.5	41.0	30.0	41.0
26-27	36.5281218671725	40.0	36.0	41.0	29.5	41.0
28-29	36.33693426432808	40.0	36.0	41.0	28.5	41.0
30-31	36.282670403895445	40.0	36.0	41.0	29.0	41.0
32-33	36.147898977912746	40.0	35.0	41.0	29.0	41.0
34-35	36.00848183361927	40.0	35.0	41.0	28.0	41.0
36-37	35.86874230950217	40.0	35.0	41.0	27.0	41.0
38-39	35.69181190321006	40.0	35.0	41.0	27.0	41.0
40-41	35.47960180392409	39.0	35.0	41.0	26.0	41.0
42-43	35.297496660752955	39.0	35.0	41.0	25.5	41.0
44-45	35.063939931549086	39.0	35.0	41.0	24.5	41.0
46-47	34.74984319421996	39.0	34.0	41.0	23.5	41.0
48-49	34.468465232373596	39.0	34.0	41.0	22.5	41.0
50-51	34.2254481313369	38.5	34.0	41.0	21.5	41.0
52-53	34.01814562916675	38.0	33.5	40.5	20.0	41.0
54-55	33.793551526700824	38.0	33.0	40.0	20.0	41.0
56-57	33.53740044367414	37.5	33.0	40.0	19.0	41.0
58-59	33.384522845710705	37.0	33.0	40.0	18.5	41.0
60-61	33.25909020497278	37.0	33.0	40.0	18.0	41.0
62-63	32.99598968852205	36.0	33.0	40.0	18.0	41.0
64-65	32.69737215141038	36.0	33.0	39.0	16.5	41.0
66-67	32.39106923128634	35.0	32.0	39.0	14.0	41.0
68-69	32.075827358735964	35.0	32.0	38.5	13.0	40.5
70-71	31.777574289113034	35.0	32.0	37.0	11.5	40.0
72-73	31.467037466790202	35.0	32.0	37.0	9.0	39.0
74-75	31.1830439319232	35.0	32.0	36.5	7.5	39.0
76-77	30.904699204718497	35.0	31.0	36.0	7.0	39.0
78-79	30.641795097371567	35.0	31.0	35.5	7.0	37.0
80-81	30.424653434382698	35.0	31.0	35.0	3.5	37.0
82-83	30.243421610262374	35.0	31.0	35.0	2.0	36.5
84-85	30.055275133496814	35.0	31.0	35.0	2.0	36.0
86-87	29.917885504014404	35.0	31.0	35.0	2.0	36.0
88-89	29.816338782322013	35.0	31.0	35.0	2.0	35.5
90-91	29.71872082725644	35.0	31.0	35.0	2.0	35.0
92-93	29.780162680516625	35.0	31.0	35.0	2.0	35.0
94-95	29.75194962340308	35.0	31.0	35.0	2.0	35.0
96-97	29.730519402705887	35.0	31.0	35.0	2.0	35.0
98-99	29.690517999783715	35.0	31.0	35.0	2.0	35.0
100-101	29.643404366010703	35.0	31.0	35.0	2.0	35.0
102-103	28.75966949491879	33.5	29.5	35.0	2.0	35.0
104-105	29.461718345837852	34.5	30.5	35.0	2.0	35.0
106-107	29.566573625647756	35.0	31.0	35.0	2.0	35.0
108-109	29.57372297548101	35.0	31.0	35.0	2.0	35.0
110-111	29.539639273637047	35.0	31.0	35.0	2.0	35.0
112-113	29.4932802950813	35.0	31.0	35.0	2.0	35.0
114-115	29.428789132029593	35.0	31.0	35.0	2.0	35.0
116-117	29.355062649243152	35.0	31.0	35.0	2.0	35.0
118-119	29.26002139456309	35.0	31.0	35.0	2.0	35.0
120-121	29.139199106806217	35.0	31.0	35.0	2.0	35.0
122-123	29.02365852874383	35.0	31.0	35.0	2.0	35.0
124-125	28.111727552514594	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29685.0
3	10730.0
4	6017.0
5	5146.0
6	5447.0
7	6696.0
8	7468.0
9	8365.0
10	9485.0
11	10388.0
12	11345.0
13	12858.0
14	16898.0
15	14063.0
16	7632.0
17	6448.0
18	5775.0
19	5138.0
20	4991.0
21	5167.0
22	6188.0
23	8305.0
24	11505.0
25	16002.0
26	20255.0
27	22196.0
28	26061.0
29	31484.0
30	35884.0
31	42900.0
32	53233.0
33	68756.0
34	94822.0
35	152204.0
36	303400.0
37	478307.0
38	149156.0
39	315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.02450438176036	17.637419425823765	12.579637777598302	24.758438414817572
2	17.165648360538828	19.675638516523215	37.1434467148813	26.015266408056657
3	19.411625411841747	24.107103703725357	28.878007139077532	27.60326374535536
4	13.553386928383638	15.491289585813007	34.96857700169909	35.98674648410426
5	14.395017158922203	37.02649239353077	33.08540751928588	15.493082928261146
6	33.7008495535897	34.87888160625852	16.437020189322403	14.983248650829381
7	29.789781643253203	31.58993271796503	19.911927652518806	18.708357986262957
8	28.242957032211375	33.08147341849998	18.82933952684632	19.846230022442327
9	27.91271103212833	14.32686012252724	18.65956601038208	39.10086283496235
10-11	26.621514260977435	24.800666675630502	26.246669983286342	22.33114908010572
12-13	27.07965046673027	22.480831556204052	26.812934929008364	23.626583048057316
14-15	24.44364163328034	23.43601619379238	24.494966987800876	27.625375185126405
16-17	24.14392135713315	26.504493523158928	24.973728710755662	24.377856408952262
18-19	24.165049797996154	25.084604272976147	27.364491401025713	23.385854528001985
20-21	25.249061734219758	24.092975642067866	27.205162091460856	23.452800532251526
22-23	24.993233103366027	23.689165891567132	27.135197040922332	24.18240396414451
24-25	24.437669747046026	24.278559524850046	27.146022142132814	24.137748585971114
26-27	24.0657000278276	24.343859103483595	27.281161650293594	24.309279218395215
28-29	24.244295106608565	24.327519330558022	27.282841632223764	24.14534393060965
30-31	24.495867217723795	24.253247332248332	27.133989382493073	24.116896067534803
32-33	24.09276147262872	24.38344495590784	27.086503183516164	24.437290387947275
34-35	24.37191100778983	24.39971303397577	27.237887700863062	23.990488257371336
36-37	24.54206382823853	24.200919248710186	26.975795689768322	24.281221233282967
38-39	24.507195178423586	24.455713343530302	26.8206327266604	24.216458751385712
40-41	24.513772452289253	24.43131195498902	26.867302030498397	24.18761356222333
42-43	24.537814487400627	24.493910261750447	26.595086994381607	24.373188256467323
44-45	24.437792241771188	24.54987276200722	26.49415114499577	24.518183851225825
46-47	24.66455975395251	24.679585465751757	26.150438946644982	24.505415833650755
48-49	24.91055678438265	24.931020994525532	25.55620261439055	24.60221960670127
50-51	24.76339117104708	25.185948697920622	25.422615992114206	24.62804413891809
52-53	24.960540398475466	25.060347829188384	25.49714420361184	24.481967568724315
54-55	24.776630152619983	25.06738221413571	25.58951398275951	24.566473650484802
56-57	24.619752716114675	25.131913876003736	25.42610177211445	24.822231635767146
58-59	24.68443812753906	24.968055745975434	25.59422451395184	24.753281612533666
60-61	24.72996439283647	25.052358316816054	25.794437330807508	24.423239959539973
62-63	24.618114684725903	25.119050925350706	25.69953473006889	24.563299659854497
64-65	24.651414061657256	24.829065213495642	25.80101609325911	24.718504631587994
66-67	24.596892415488522	25.06248931228871	25.65913033819207	24.681487934030695
68-69	24.78490774326277	25.02931581235424	25.51240180920442	24.673374635178565
70-71	24.609697811232508	24.867175795666313	25.789045200423864	24.734081192677312
72-73	24.893905186594445	25.16770391149806	25.358363241395704	24.5800276605118
74-75	24.62052435359123	25.36069297955072	25.302313235246398	24.71646943161165
76-77	24.673961784526842	25.032257423299825	25.379737251813694	24.914043540359636
78-79	24.67899755021561	25.166235369343227	25.343500469695503	24.811266610745662
80-81	24.647183637576603	25.3099293753351	25.223483008041235	24.81940397904706
82-83	24.81489839215175	25.158648081578583	25.250460817504578	24.77599270876509
84-85	24.56099067478802	25.254104060841737	25.14144500007454	25.043460264295703
86-87	24.34745345050047	25.30586246872974	25.313374931525512	25.033309149244282
88-89	24.515780297241058	25.090728158149687	25.2667176899883	25.126773854620954
90-91	24.397005724762405	25.087929357180272	25.39664675783881	25.118418160218514
92-93	24.51673664383201	25.27561181789802	25.252666089077362	24.954985449192602
94-95	24.404639243812262	25.031414774107386	25.384686660464094	25.17925932161626
96-97	24.441370908672145	25.07314424073501	25.322667670567107	25.162817180025733
98-99	24.358975483134042	25.107654589886653	25.19437451609141	25.338995410887893
100-101	24.323601441221918	25.136174190715778	25.320243929674074	25.21998043838823
102-103	24.480483324345535	24.960738665509215	25.430237877067373	25.128540133077877
104-105	24.257416626794292	25.178022718045863	25.215873462185645	25.3486871929742
106-107	24.350393798596432	25.127381358436367	25.214859682488466	25.30736516047874
108-109	24.357290029561444	25.058672681859	25.18990420595719	25.39413308262236
110-111	24.380443315860944	25.154249812503544	25.173277122392662	25.292029749242857
112-113	24.313644905598498	25.133357049047977	25.271120602593513	25.28187744276002
114-115	24.030616002815265	25.358689031340866	25.28123418021201	25.329460785631863
116-117	24.11412052193434	25.371018847628847	25.092688943971563	25.42217168646525
118-119	24.143783211495126	24.992933491217798	25.230870953418922	25.632412343868154
120-121	23.881088671114135	25.155322347945443	25.335547714417327	25.628041266523095
122-123	23.898321630576348	25.214268010671603	25.21333272539791	25.67407763335414
124-125	23.91139425906225	25.335972643323917	25.128903163225303	25.623729934388535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	38.0
2	62.0
3	94.5
4	81.0
5	69.0
6	140.0
7	294.5
8	468.0
9	548.0
10	483.0
11	453.0
12	531.5
13	640.0
14	764.5
15	945.5
16	1137.5
17	1310.5
18	1512.5
19	1689.5
20	1905.0
21	2185.5
22	2510.5
23	2810.5
24	3171.5
25	3807.0
26	4904.0
27	6084.0
28	6848.5
29	8084.5
30	10124.5
31	12156.5
32	14731.0
33	17128.0
34	19290.5
35	21863.0
36	25143.5
37	28535.0
38	30476.0
39	32601.0
40	35041.0
41	36748.0
42	38949.5
43	43864.0
44	48695.0
45	51960.0
46	56055.0
47	59651.5
48	64273.0
49	69465.0
50	70139.0
51	69525.0
52	70030.5
53	70850.0
54	72204.5
55	71794.0
56	68084.0
57	62318.0
58	58779.0
59	55210.5
60	49617.0
61	44212.5
62	38460.5
63	30365.0
64	22688.0
65	17967.0
66	13852.0
67	10468.0
68	8527.0
69	7041.0
70	5933.0
71	4887.5
72	3988.0
73	3762.5
74	3143.5
75	2475.0
76	1737.5
77	965.5
78	575.5
79	351.5
80	197.0
81	94.0
82	59.5
83	29.0
84	16.5
85	8.0
86	6.0
87	4.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11930683953785405
2	0.0018121078028777442
3	0.02396658707031855
4	0.057285988607102885
5	0.030747377558506237
6	0.023674311618241496
7	0.017711892395869563
8	0.006313149764864399
9	0.004384131781155832
10-11	0.008066802477326732
12-13	0.00531941322780241
14-15	0.0034780778797169607
16-17	0.011544880357043692
18-19	0.006079329403202755
20-21	0.014350724696983425
22-23	0.01063882645560482
24-25	0.015519826505291646
26-27	0.010697281546020232
28-29	0.016104377409445758
30-31	0.024931096062172836
32-33	0.015402916324460825
34-35	0.023849676889487727
36-37	0.01733193430816939
38-39	0.023557401437410672
40-41	0.011632562992666809
42-43	0.010141958187073826
44-45	0.019728593015201246
46-47	0.01832567084523138
48-49	0.024083497251149374
50-51	0.017361161853377098
52-53	0.02463882061009578
54-55	0.018880994204177784
56-57	0.02417117988677249
58-59	0.01832567084523138
60-61	0.022505209809933275
62-63	0.024405000248434134
64-65	0.01990395828644748
66-67	0.01300625761742897
68-69	0.026918569136296814
70-71	0.01622128759027658
72-73	0.01955322774395501
74-75	0.02113151518517111
76-77	0.01496450314634524
78-79	0.03299789853949957
80-81	0.023732766708656906
82-83	0.009820455189789065
84-85	0.014292269606568014
86-87	0.013093940253052085
88-89	0.022563664900348685
90-91	0.014672227694268184
92-93	0.009177449195219543
94-95	0.007862209660872792
96-97	0.0031565748824321995
98-99	0.002075155709747094
100-101	0.012977030072221264
102-103	0.003653443150963194
104-105	0.002835071885147438
106-107	3.2150299728476105E-4
108-109	0.022154479267440807
110-111	9.352814466465775E-4
112-113	0.010258868367904649
114-115	0.0023966587070318552
116-117	0.00906053901438872
118-119	0.010609598910397115
120-121	0.004617952142817476
122-123	4.0918563290787764E-4
124-125	0.024521910429264958
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1710715.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.38809150243389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.36215966285498	45.31468812402269
2	10.17816190280345	11.478542494037352
3	3.386963120566777	5.729531590736654
4	1.6355433872598124	3.689006807080279
5	1.0118349573999188	2.852772108161396
6	0.6652796609714526	2.250831023853988
7	0.47507169887127926	1.8751870498319292
8	0.3303824560626234	1.4903708930606443
9	0.26354308441928503	1.3374622403161476
>10	1.5624010843192604	16.155907363197908
>50	0.09022137674890507	3.4808751410029592
>100	0.03718776002299905	3.552962659774763
>500	8.332317995525776E-4	0.27826481853636753
>1k	4.166158997762888E-4	0.5135976863867737
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3268	0.19103123547756348	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2885	0.16864293584846102	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.84550904154111E-5
10-11	0.0	0.0	0.0	0.0	5.84550904154111E-5
12-13	0.0	0.0	0.0	0.0	8.768263562311665E-5
14-15	0.0	0.0	0.0	2.922754520770555E-5	2.922754520770555E-4
16-17	0.0	0.0	0.0	5.84550904154111E-5	4.091856329078777E-4
18-19	0.0	0.0	0.0	5.84550904154111E-5	4.676407233232888E-4
20-21	0.0	0.0	0.0	1.169101808308222E-4	4.676407233232888E-4
22-23	0.0	0.0	0.0	1.169101808308222E-4	4.676407233232888E-4
24-25	0.0	0.0	0.0	1.753652712462333E-4	4.676407233232888E-4
26-27	0.0	0.0	0.0	2.338203616616444E-4	4.676407233232888E-4
28-29	0.0	0.0	0.0	0.0010814191726851053	4.676407233232888E-4
30-31	0.0	0.0	0.0	0.0037411257865863105	4.968682685309943E-4
32-33	0.0	0.0	0.0	0.010521916274773998	5.553233589464055E-4
34-35	0.0	0.0	0.0	0.01858871875210073	6.430059945695221E-4
36-37	0.0	0.0	0.0	0.03188725182160675	6.430059945695221E-4
38-39	0.0	0.0	0.0	0.054100186179462974	6.430059945695221E-4
40-41	0.0	0.0	0.0	0.07707303671271953	6.430059945695221E-4
42-43	0.0	0.0	0.0	0.09946133634182198	6.722335397772276E-4
44-45	0.0	0.0	0.0	0.12556153421230304	7.014610849849332E-4
46-47	0.0	0.0	0.0	0.15668886985850944	7.014610849849332E-4
48-49	0.0	0.0	0.0	0.18875148695136243	7.599161754003443E-4
50-51	0.0	0.0	0.0	0.22294771484437792	7.599161754003443E-4
52-53	0.0	0.0	0.0	0.2604758828910718	8.183712658157554E-4
54-55	0.0	0.0	0.0	0.29841323657067365	8.768263562311665E-4
56-57	0.0	0.0	0.0	0.3424883747438936	8.768263562311665E-4
58-59	0.0	0.0	0.0	0.39711465673709534	8.768263562311665E-4
60-61	0.0	0.0	0.0	0.45270544772215127	8.768263562311665E-4
62-63	0.0	0.0	0.0	0.5085885141592843	8.768263562311665E-4
64-65	0.0	0.0	0.0	0.5696448560981812	8.768263562311665E-4
66-67	0.0	0.0	0.0	0.6366051621690345	8.768263562311665E-4
68-69	0.0	0.0	0.0	0.7152272587777626	8.768263562311665E-4
70-71	0.0	0.0	0.0	0.8014192895952862	9.352814466465776E-4
72-73	0.0	0.0	0.0	0.8893649731252722	9.645089918542832E-4
74-75	0.0	0.0	0.0	0.9832438483324224	9.937365370619887E-4
76-77	0.0	0.0	0.0	1.0891352446199396	9.937365370619887E-4
78-79	0.0	0.0	0.0	1.2076529404371856	9.937365370619887E-4
80-81	0.0	0.0	0.0	1.3395860795047685	0.0010229640822696942
82-83	0.0	5.84550904154111E-5	0.0	1.4833563743814722	0.0011106467178928108
84-85	0.0	5.84550904154111E-5	0.0	1.6281204057952376	0.0011106467178928108
86-87	0.0	5.84550904154111E-5	0.0	1.7851015511058241	0.0011106467178928108
88-89	0.0	5.84550904154111E-5	0.0	1.9653185948565368	0.001169101808308222
90-91	0.0	5.84550904154111E-5	0.0	2.165205776532035	0.001169101808308222
92-93	0.0	5.84550904154111E-5	0.0	2.380583557167617	0.001169101808308222
94-95	0.0	5.84550904154111E-5	0.0	2.5994978707733316	0.0012275568987236331
96-97	0.0	5.84550904154111E-5	0.0	2.830541615640244	0.0013152395343467497
98-99	0.0	5.84550904154111E-5	0.0	3.0852596721253978	0.0013736946247621608
100-101	0.0	5.84550904154111E-5	0.0	3.373589405599413	0.0014029221699698663
102-103	0.0	1.169101808308222E-4	0.0	3.6816477320886296	0.0015782874412160997
104-105	0.0	1.169101808308222E-4	0.0	4.008733190508062	0.0015782874412160997
106-107	0.0	1.169101808308222E-4	0.0	4.341664157969037	0.0015782874412160997
108-109	0.0	1.169101808308222E-4	0.0	4.698064844231798	0.0015782874412160997
110-111	0.0	1.169101808308222E-4	0.0	5.092665932081031	0.0015782874412160997
112-113	0.0	1.169101808308222E-4	0.0	5.516377654957138	0.0015782874412160997
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2775	0.0	78.38919	1
GTATCAA	5635	0.0	63.034546	1
TCAACGC	7680	0.0	45.74283	4
CAACGCA	7910	0.0	44.314148	5
ATCAACG	8085	0.0	43.59874	3
TATCAAC	8160	0.0	43.343956	2
AACGCAG	8200	0.0	42.8095	6
ACGCAGA	9675	0.0	36.403854	7
CGCAGAG	10215	0.0	34.360928	8
GTACATG	8960	0.0	33.656494	1
GCAGAGT	10630	0.0	32.57079	9
TACATGG	9460	0.0	31.282215	2
ACATGGG	9580	0.0	30.766066	3
CATGGGG	7535	0.0	27.57874	4
AGAGTAC	9950	0.0	27.47308	10-11
CAGAGTA	10335	0.0	27.284296	10-11
GTGGTAT	1035	0.0	27.063494	1
GAGTACT	5815	0.0	25.67883	12-13
ATGGGGG	4170	0.0	25.260435	5
TGGTATC	1230	0.0	24.68865	2
>>END_MODULE