FastQCFastQC Report
Mon 15 Aug 2016
SRR2921494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669028
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.067.622891
GTATCAA34800.055.404131
TCAACGC44450.042.6951074
CAACGCA45300.041.8939865
AACGCAG48150.039.782566
ATCAACG48050.039.4951253
GTACATG89450.039.3172841
TATCAAC48650.038.885752
TACATGG93800.037.2290082
ACATGGG92900.036.6931573
CATGGGG76050.033.9507754
ACGCAGA57900.032.9796947
CGCAGAG61250.031.2702168
ATGGGGG41600.030.0320635
GCAGAGT66950.027.5417929
AGAGTAC60050.024.41659210-11
CAGAGTA64800.023.08575410-11
TATACCG2352.9139665E-722.7842045
GTATAGT4000.022.3155521
GTATAGG5150.021.9544581
Produced by FastQC (version 0.11.5)