##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921494_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669028
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83609022736587	31.0	30.0	34.0	25.0	34.0
2	29.935858475711612	31.0	30.0	34.0	25.0	34.0
3	30.028012112439097	31.0	30.0	34.0	25.0	34.0
4	33.408862523576595	37.0	35.0	37.0	27.0	37.0
5	33.42823547597764	37.0	35.0	37.0	27.0	37.0
6	33.502633868335344	37.0	35.0	37.0	27.0	37.0
7	33.390583621125586	37.0	35.0	37.0	27.0	37.0
8	33.36511490520231	37.0	35.0	37.0	27.0	37.0
9	34.839334630695234	39.0	35.0	39.0	27.0	39.0
10-11	34.72241088825352	39.0	35.0	39.0	27.0	39.0
12-13	34.57704574159331	39.0	35.0	39.0	25.5	39.0
14-15	35.73608321729773	40.0	36.0	41.0	25.5	41.0
16-17	35.66474588802585	40.0	36.0	41.0	25.0	41.0
18-19	35.679932871108214	40.0	36.0	41.0	25.0	41.0
20-21	35.70088278926417	40.0	36.0	41.0	25.0	41.0
22-23	35.67495033037193	40.0	36.0	41.0	25.0	41.0
24-25	35.64209348195477	40.0	36.0	41.0	25.0	41.0
26-27	35.56120987784507	40.0	35.0	41.0	25.0	41.0
28-29	35.521196768418505	40.0	35.0	41.0	25.0	41.0
30-31	35.425341276479486	40.0	35.0	41.0	25.0	41.0
32-33	35.32222587038684	40.0	35.0	41.0	25.0	41.0
34-35	35.16785967641046	40.0	35.0	41.0	24.0	41.0
36-37	35.03352699894789	40.0	35.0	41.0	24.0	41.0
38-39	34.90903447994881	39.0	35.0	41.0	23.5	41.0
40-41	34.76192190903927	39.0	34.0	41.0	23.0	41.0
42-43	34.56341595227881	39.0	34.0	41.0	22.0	41.0
44-45	34.37741308114663	39.0	34.0	41.0	20.5	41.0
46-47	34.124193243013295	39.0	33.0	41.0	19.0	41.0
48-49	33.98018517364598	39.0	33.0	41.0	18.0	41.0
50-51	33.78401530711288	38.0	33.0	40.5	15.0	41.0
52-53	33.58302497022218	38.0	33.0	40.0	14.5	41.0
54-55	33.38776341679109	38.0	33.0	40.0	13.5	41.0
56-57	33.14415186563677	37.0	33.0	40.0	12.5	41.0
58-59	32.90149895627874	37.0	32.0	40.0	10.5	41.0
60-61	32.59063029499805	36.5	32.0	40.0	8.0	41.0
62-63	32.278606769928366	36.0	32.0	39.5	7.5	41.0
64-65	31.970888145675204	35.0	31.5	39.0	7.0	41.0
66-67	31.919754791411528	35.0	32.0	39.0	4.0	41.0
68-69	31.666697323232444	35.0	32.0	38.5	2.0	40.5
70-71	31.367081918338098	35.0	31.0	37.0	2.0	40.0
72-73	31.039093412453234	35.0	31.0	37.0	2.0	39.0
74-75	30.73714221690709	35.0	31.0	36.5	2.0	39.0
76-77	30.459149576879476	35.0	31.0	36.0	2.0	38.5
78-79	30.188389589629416	35.0	31.0	35.5	2.0	37.0
80-81	29.95063324282157	35.0	30.5	35.0	2.0	37.0
82-83	29.758105915538863	35.0	30.0	35.0	2.0	36.5
84-85	29.56197589255543	35.0	30.0	35.0	2.0	36.0
86-87	29.413645546989024	35.0	30.0	35.0	2.0	36.0
88-89	29.29342287846579	35.0	30.0	35.0	2.0	35.5
90-91	29.186569068943122	34.0	30.0	35.0	2.0	35.0
92-93	29.08855453593349	34.0	30.0	35.0	2.0	35.0
94-95	29.02415208133117	34.0	30.0	35.0	2.0	35.0
96-97	28.92350457871288	34.0	30.0	35.0	2.0	35.0
98-99	28.862487327953755	34.0	30.0	35.0	2.0	35.0
100-101	28.802041367790117	34.0	29.5	35.0	2.0	35.0
102-103	27.83184494208605	33.5	28.0	34.5	2.0	35.0
104-105	28.651057981052446	34.0	29.5	35.0	2.0	35.0
106-107	28.971103840079376	35.0	30.0	35.0	2.0	35.0
108-109	29.010103784957472	35.0	30.0	35.0	2.0	35.0
110-111	28.955639749602764	35.0	30.0	35.0	2.0	35.0
112-113	28.88546657096226	35.0	30.0	35.0	2.0	35.0
114-115	28.767005406739734	35.0	30.0	35.0	2.0	35.0
116-117	28.703986691655263	35.0	30.0	35.0	2.0	35.0
118-119	28.55274267417922	35.0	30.0	35.0	2.0	35.0
120-121	28.389656135187664	35.0	30.0	35.0	2.0	35.0
122-123	28.26663093728805	35.0	30.0	35.0	2.0	35.0
124-125	27.42925523118845	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61433.0
3	10669.0
4	7242.0
5	6154.0
6	6551.0
7	7610.0
8	8232.0
9	8645.0
10	9241.0
11	9560.0
12	9873.0
13	10361.0
14	11514.0
15	10080.0
16	7231.0
17	6312.0
18	5762.0
19	5329.0
20	5234.0
21	5535.0
22	6505.0
23	8883.0
24	12671.0
25	17250.0
26	20867.0
27	22265.0
28	25927.0
29	31198.0
30	34759.0
31	41724.0
32	52664.0
33	68378.0
34	96512.0
35	153101.0
36	293748.0
37	442874.0
38	126985.0
39	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9736489276709	17.103743487300648	12.049655043449961	24.872952541578485
2	16.96243375430526	19.263645729141633	37.96999758480018	25.803922931752926
3	19.310750296062253	24.134946852630517	28.723012787012575	27.83129006429465
4	13.391816335324918	15.081878000789922	35.40259355752017	36.123712106364984
5	14.382749235677455	37.16019037789689	33.370193428594206	15.086866957831452
6	33.74639002477981	34.45825495985719	17.220885527371074	14.574469487991927
7	30.00230163116121	31.04618726406782	20.509871240519256	18.441639864251712
8	28.21062990828184	33.21470247488584	19.244150817420156	19.33051679941216
9	27.84265733008423	14.494516835454721	18.661757848974148	39.001067985486905
10-11	26.495980580924055	24.71664781198259	26.826074904290625	21.96129670280273
12-13	26.686121569769742	22.40694191219042	26.970033695460643	23.936902822579196
14-15	24.196718638980464	23.662761026548342	24.166062076093606	27.974458258377588
16-17	24.387996312754204	26.578347380269403	24.69942233948314	24.33423396749326
18-19	24.521139171457172	25.768269363337186	26.180292885273616	23.530298579932023
20-21	25.539490280901838	24.78630813353988	25.83426455706523	23.83993702849305
22-23	25.302330795314248	24.34013552020799	25.77992158447204	24.577612100005723
24-25	24.813863127722957	24.85505035093073	25.740725530622733	24.590360990723582
26-27	24.41320632682776	24.928713192959613	25.864657868283203	24.79342261192943
28-29	24.48913960859912	24.98063855600792	25.90165585303045	24.628565982362506
30-31	24.89820237960367	24.85138716303984	25.78751166633998	24.462898791016514
32-33	24.517399341235826	24.906279421157965	25.72552332006593	24.85079791754028
34-35	24.720561188236434	25.037094178004704	25.830254763803513	24.41208986995535
36-37	24.86881861976837	24.807255461032828	25.680439250440067	24.643486668758737
38-39	24.854501985535325	25.007433767529303	25.578934621213573	24.559129625721795
40-41	24.7792809250225	24.940348828601152	25.745898148378487	24.534472097997863
42-43	24.670583298363407	24.943978178766262	25.602751633595105	24.782686889275222
44-45	24.60882339628908	24.979070204514002	25.653679552466567	24.758426846730348
46-47	24.63381031518212	24.988968745578504	25.561155116223862	24.816065823015514
48-49	24.810377811738817	25.243376073691525	25.24250690018861	24.703739214381045
50-51	24.75662594846944	25.349205797337753	25.163857874349453	24.730310379843353
52-53	24.889871670821208	25.173165856740848	25.301788791010367	24.63517368142758
54-55	24.747147788693297	25.120079699472562	25.474339539174483	24.658432972659654
56-57	24.648008259769142	25.21486338863323	25.25433405611308	24.88279429548455
58-59	24.75016633599271	25.050680029490923	25.318945519717555	24.88020811479881
60-61	24.809602495200732	25.2176677856472	25.419228125189168	24.5535015939629
62-63	24.67721744519792	25.32570461316593	25.389510379900553	24.607567561735603
64-65	24.710284351099283	24.998771248411614	25.453199549257953	24.83774485123115
66-67	24.647216011473926	25.225257622438647	25.337215912581435	24.79031045350599
68-69	24.692350244555563	25.271600210244067	25.313403298818066	24.72264624638231
70-71	24.60464369078347	25.083201358076344	25.484522029777455	24.827632921362728
72-73	24.78532078420843	25.321868991738867	25.23514625995483	24.657663964097868
74-75	24.671039914552942	25.409321437315896	25.2180605208039	24.701578127327263
76-77	24.63987437931334	25.137023041447037	25.30657500831578	24.916527570923847
78-79	24.59664755986757	25.264906060235564	25.346984985460136	24.791461394436734
80-81	24.660905470639175	25.407293985802763	25.142646534442825	24.789154009115236
82-83	24.785660480928325	25.286430227520196	25.18788228899685	24.740027002554633
84-85	24.573710374443326	25.38834161657619	25.11891524724933	24.91903276173115
86-87	24.413741692189596	25.490348827663155	25.3046721489776	24.79123733116965
88-89	24.493035113288776	25.31872956439194	25.250501002004004	24.937734320315272
90-91	24.563399338638643	25.265028413610608	25.263380553028004	24.908191694722742
92-93	24.61737048125625	25.463739785559675	25.189127448888847	24.72976228429523
94-95	24.606511776183012	25.238915335164595	25.26689964956687	24.887673239085515
96-97	24.59389132984692	25.213147317850687	25.241909509949174	24.951051842353227
98-99	24.44579799366455	25.300631237965238	25.163472027520523	25.090098740849687
100-101	24.462849633850315	25.36529580496305	25.26546676070331	24.906387800483326
102-103	24.517543110666914	25.138059890414915	25.420246427130174	24.924150571787997
104-105	24.40670481308127	25.385990525013025	25.19329111242866	25.014013549477042
106-107	24.386766322413525	25.344475346767304	25.332820813498408	24.935937517320763
108-109	24.466051880417194	25.315844712891185	25.221256835299943	24.996846571391675
110-111	24.431012684774075	25.501668733859418	25.267386888605277	24.79993169276123
112-113	24.43330461157922	25.43603936997494	25.302357380911577	24.82829863753426
114-115	24.20590741368793	25.68114298999452	25.303555785116536	24.809393811201005
116-117	24.274505856263147	25.720101360359564	25.11152575623301	24.893867027144275
118-119	24.442586574870294	25.355084821856234	25.228507447043963	24.973821156229516
120-121	24.124706988003535	25.497703254947268	25.388390276195654	24.989199480853543
122-123	24.249518897398538	25.618601155605152	25.24028160758689	24.89159833940942
124-125	24.187379322844812	25.68585068313194	25.222720034991692	24.904049959031557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	124.0
2	159.5
3	167.0
4	147.0
5	154.0
6	178.5
7	208.0
8	248.0
9	303.5
10	343.0
11	377.5
12	423.0
13	480.5
14	551.0
15	621.5
16	673.0
17	780.0
18	888.5
19	970.0
20	1126.0
21	1270.5
22	1470.0
23	1768.0
24	2135.5
25	2725.0
26	3699.0
27	4839.5
28	5739.0
29	7019.0
30	8992.0
31	10865.5
32	13248.0
33	15775.0
34	18348.0
35	21288.0
36	24398.5
37	27864.0
38	30286.0
39	32878.5
40	35664.5
41	37733.0
42	40244.5
43	44300.5
44	48824.0
45	52391.0
46	55919.5
47	59330.5
48	64164.5
49	68676.0
50	69199.0
51	69305.0
52	69613.5
53	69833.5
54	70518.0
55	69772.5
56	66622.0
57	61288.0
58	57115.5
59	53036.0
60	47610.0
61	42262.0
62	36388.5
63	28938.5
64	22113.0
65	17500.5
66	13684.5
67	10763.5
68	8714.0
69	7017.5
70	5968.5
71	4893.0
72	3790.0
73	3281.5
74	2731.5
75	2146.5
76	1539.5
77	933.5
78	581.0
79	393.0
80	222.5
81	98.5
82	61.5
83	38.5
84	21.5
85	18.5
86	20.5
87	18.0
88	15.5
89	13.0
90	9.5
91	9.0
92	8.5
93	8.5
94	9.5
95	7.0
96	3.0
97	3.5
98	4.5
99	8.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03337271753379811
2	0.025703583163374132
3	0.02756095164371119
4	0.03049679214488912
5	0.03337271753379811
6	0.043977692405400025
7	0.03870516252573354
8	0.03277356641110874
9	0.027920442317324816
10-11	0.033881995988084085
12-13	0.0331929721969913
14-15	0.03256386351816746
16-17	0.03439127444237005
18-19	0.04781225959061202
20-21	0.058237489125407126
22-23	0.051736699444227416
24-25	0.062012141198350176
26-27	0.04595489111027496
28-29	0.04577514577346815
30-31	0.04595489111027496
32-33	0.054193219047253854
34-35	0.038315714295985445
36-37	0.049280179841200984
38-39	0.05775816822725562
40-41	0.04718315091178818
42-43	0.05560122418557388
44-45	0.05700922932389391
46-47	0.062311716759694866
48-49	0.04652408467682986
50-51	0.04877090138691502
52-53	0.058357319349944994
54-55	0.04559540043666133
56-57	0.04197053614439063
58-59	0.043019050609097026
60-61	0.032653736186570866
62-63	0.04095197923581869
64-65	0.04002329499565016
66-67	0.03292335419178108
68-69	0.02941832012404825
70-71	0.04709327824338477
72-73	0.029478235236317183
74-75	0.03753681783648926
76-77	0.030736452593964875
78-79	0.030676537481695933
80-81	0.023546639121692386
82-83	0.017794788343874397
84-85	0.01782474590000887
86-87	0.015997334975806278
88-89	0.022288421764044702
90-91	0.011833234673115131
92-93	0.018693515027908458
94-95	0.01413996649546922
96-97	0.010215526641853823
98-99	0.009856035968240197
100-101	0.01048514464706404
102-103	0.005781808333952456
104-105	0.006440874568910767
106-107	0.008957309284206136
108-109	0.012552216020342379
110-111	0.005871681002355862
112-113	0.008507945942189106
114-115	0.008507945942189106
116-117	0.007848879707230794
118-119	0.24196718089810357
120-121	0.3543080164023611
122-123	0.027860527205055875
124-125	0.0036248642922707104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1669028.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.18624188641473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94185742872475	46.51516253197058
2	10.374534853137371	12.073103888473824
3	3.5254995914695892	6.1540671599911745
4	1.7490138606764227	4.070741742400416
5	1.0213640541201439	2.971466795356194
6	0.6780757330762	2.367280717324786
7	0.49118297995584215	2.000606417754044
8	0.3506818145414062	1.6323885508858473
9	0.27944145266274284	1.4633683161952917
>10	1.49093495810381	15.355626058173991
>50	0.07509186954400308	2.952631258884956
>100	0.021698554365733577	2.1271701989605036
>500	3.1142481101363893E-4	0.10280442743328715
>1k	3.1142481101363893E-4	0.21358193619512533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.991511226893737E-5
6	0.0	0.0	0.0	0.0	5.991511226893737E-5
7	0.0	0.0	0.0	0.0	5.991511226893737E-5
8	0.0	0.0	0.0	0.0	5.991511226893737E-5
9	0.0	0.0	0.0	0.0	1.1983022453787474E-4
10-11	0.0	0.0	0.0	0.0	1.1983022453787474E-4
12-13	0.0	0.0	0.0	0.0	2.097028929412808E-4
14-15	0.0	0.0	0.0	2.9957556134468685E-5	3.2953311747915554E-4
16-17	0.0	0.0	0.0	8.987266840340605E-5	3.594906736136242E-4
18-19	0.0	0.0	0.0	1.1983022453787474E-4	4.194057858825616E-4
20-21	0.0	0.0	0.0	1.1983022453787474E-4	5.092784542859676E-4
22-23	0.0	0.0	0.0	1.1983022453787474E-4	6.291086788238424E-4
24-25	0.0	0.0	0.0	1.797453368068121E-4	6.590662349583111E-4
26-27	0.0	0.0	0.0	3.2953311747915554E-4	6.590662349583111E-4
28-29	0.0	0.0	0.0	0.0013480900260510907	6.590662349583111E-4
30-31	0.0	0.0	0.0	0.0055421478848767065	6.890237910927798E-4
32-33	0.0	0.0	0.0	0.011833234673115131	7.189813472272484E-4
34-35	0.0	0.0	0.0	0.02052092595211105	7.189813472272484E-4
36-37	0.0	0.0	0.0	0.03310309952858789	7.189813472272484E-4
38-39	0.0	0.0	0.0	0.05464258238927088	7.189813472272484E-4
40-41	0.0	0.0	0.0	0.07770990061281177	7.788964594961858E-4
42-43	0.0	0.0	0.0	0.09553464651282063	8.088540156306544E-4
44-45	0.0	0.0	0.0	0.11935090363972325	8.388115717651232E-4
46-47	0.0	0.0	0.0	0.14769075174293061	8.687691278995919E-4
48-49	0.0	0.0	0.0	0.18004491236815678	0.0010185569085719352
50-51	0.0	0.0	0.0	0.21332775723355152	0.0010185569085719352
52-53	0.0	0.0	0.0	0.2450827667360883	0.0010784720208408727
54-55	0.0	0.0	0.0	0.2824997543480397	0.0011983022453787473
56-57	0.0	0.0	0.0	0.3221036435578073	0.0011983022453787473
58-59	0.0	0.0	0.0	0.37057496938337764	0.0011983022453787473
60-61	0.0	0.0	0.0	0.42701500514071666	0.0011983022453787473
62-63	0.0	0.0	0.0	0.4839942769084761	0.0013181324699166222
64-65	0.0	0.0	0.0	0.5403743975535461	0.0013780475821855594
66-67	0.0	0.0	0.0	0.606071318156436	0.0013780475821855594
68-69	0.0	5.991511226893737E-5	0.0	0.6757226361690756	0.0013780475821855594
70-71	0.0	5.991511226893737E-5	0.0	0.7583156184318058	0.0013780475821855594
72-73	1.1983022453787474E-4	5.991511226893737E-5	0.0	0.8403993222402499	0.001617708031261309
74-75	1.1983022453787474E-4	5.991511226893737E-5	0.0	0.9251192909885275	0.0016776231435302463
76-77	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.022002027527399	0.0016776231435302463
78-79	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.1332344334546813	0.0017375382557991838
80-81	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.251207289512219	0.0018573684803370586
82-83	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.3777180490680805	0.0021569440416817453
84-85	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.5080633758091535	0.0021569440416817453
86-87	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.6449694073436754	0.0022168591539506828
88-89	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.8032950915143426	0.00227677426621962
90-91	1.1983022453787474E-4	5.991511226893737E-5	0.0	1.978846370462329	0.00227677426621962
92-93	1.1983022453787474E-4	5.991511226893737E-5	0.0	2.170544772166794	0.002336689378488557
94-95	1.1983022453787474E-4	5.991511226893737E-5	0.0	2.363830924346386	0.002336689378488557
96-97	1.1983022453787474E-4	5.991511226893737E-5	0.0	2.566254131146991	0.0025164347152953695
98-99	1.1983022453787474E-4	5.991511226893737E-5	0.0	2.790756056818699	0.002576349827564307
100-101	1.1983022453787474E-4	5.991511226893737E-5	0.0	3.0412910987712607	0.002576349827564307
102-103	1.1983022453787474E-4	5.991511226893737E-5	0.0	3.3245697495787967	0.0027560951643711192
104-105	1.1983022453787474E-4	5.991511226893737E-5	0.0	3.616356346328522	0.002816010276640056
106-107	1.1983022453787474E-4	5.991511226893737E-5	0.0	3.916021780341612	0.002935840501177931
108-109	1.1983022453787474E-4	5.991511226893737E-5	0.0	4.226052528777229	0.003085628281850275
110-111	1.1983022453787474E-4	5.991511226893737E-5	0.0	4.577604450015219	0.003175500950253681
112-113	1.1983022453787474E-4	5.991511226893737E-5	0.0	4.943386210417081	0.003175500950253681
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1760	0.0	67.62289	1
GTATCAA	3480	0.0	55.40413	1
TCAACGC	4445	0.0	42.695107	4
CAACGCA	4530	0.0	41.893986	5
AACGCAG	4815	0.0	39.78256	6
ATCAACG	4805	0.0	39.495125	3
GTACATG	8945	0.0	39.317284	1
TATCAAC	4865	0.0	38.88575	2
TACATGG	9380	0.0	37.229008	2
ACATGGG	9290	0.0	36.693157	3
CATGGGG	7605	0.0	33.950775	4
ACGCAGA	5790	0.0	32.979694	7
CGCAGAG	6125	0.0	31.270216	8
ATGGGGG	4160	0.0	30.032063	5
GCAGAGT	6695	0.0	27.541792	9
AGAGTAC	6005	0.0	24.416592	10-11
CAGAGTA	6480	0.0	23.085754	10-11
TATACCG	235	2.9139665E-7	22.784204	5
GTATAGT	400	0.0	22.315552	1
GTATAGG	515	0.0	21.954458	1
>>END_MODULE