FastQCFastQC Report
Mon 15 Aug 2016
SRR2921494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669028
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.1653057947499982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.16446698317823308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.082.179361
GTATCAA49500.059.603921
TCAACGC66800.043.581194
CAACGCA68500.042.564835
ATCAACG70400.041.268043
TATCAAC71300.040.9976232
AACGCAG71050.040.9460566
ACGCAGA84400.034.46947
GTACATG83800.033.7139131
CGCAGAG88950.032.6383488
TACATGG87300.031.983452
ACATGGG87600.031.1263433
GCAGAGT95700.030.147979
CATGGGG70950.029.704184
GTATAGG6350.029.0979771
AGAGTAC88800.026.73013910-11
GCGTAAT900.00944944126.4906351
CAGAGTA93300.026.23792610-11
ATGGGGG37100.025.9828345
GAGTACT54650.024.87352612-13
Produced by FastQC (version 0.11.5)