##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921494_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1669028 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35420256580477 31.0 31.0 34.0 28.0 34.0 2 31.42262921892263 33.0 31.0 34.0 28.0 34.0 3 31.505998701040365 33.0 31.0 34.0 28.0 34.0 4 35.01887625612033 37.0 35.0 37.0 32.0 37.0 5 34.69244374570109 37.0 35.0 37.0 32.0 37.0 6 34.671801791222194 37.0 35.0 37.0 31.0 37.0 7 34.523457365604415 37.0 35.0 37.0 30.0 37.0 8 34.494883848563354 37.0 35.0 37.0 30.0 37.0 9 36.0842214750142 39.0 37.0 39.0 31.0 39.0 10-11 35.956172694526394 39.0 37.0 39.0 30.0 39.0 12-13 35.86139028224811 39.0 37.0 39.0 30.0 39.0 14-15 37.056524216490075 40.0 37.0 41.0 30.5 41.0 16-17 37.033661807950494 40.0 37.0 41.0 30.0 41.0 18-19 37.05602811936049 40.0 37.0 41.0 30.0 41.0 20-21 37.033528496825696 40.0 37.0 41.0 30.0 41.0 22-23 36.97193576141323 40.0 37.0 41.0 30.0 41.0 24-25 36.9088864896215 40.0 37.0 41.0 30.0 41.0 26-27 36.78383975583394 40.0 36.5 41.0 30.0 41.0 28-29 36.594581996227745 40.0 36.0 41.0 30.0 41.0 30-31 36.54286027556159 40.0 36.0 41.0 30.0 41.0 32-33 36.42028474057955 40.0 36.0 41.0 30.0 41.0 34-35 36.292096357880155 40.0 35.0 41.0 29.0 41.0 36-37 36.16154821848405 40.0 35.0 41.0 29.0 41.0 38-39 35.988097563372214 40.0 35.0 41.0 28.0 41.0 40-41 35.78538047294593 40.0 35.0 41.0 27.0 41.0 42-43 35.61252926853234 39.5 35.0 41.0 27.0 41.0 44-45 35.39284601576486 39.0 35.0 41.0 26.0 41.0 46-47 35.10561716160544 39.0 35.0 41.0 24.5 41.0 48-49 34.84357961639948 39.0 34.5 41.0 24.0 41.0 50-51 34.614544812909074 39.0 34.0 41.0 23.0 41.0 52-53 34.41426237306983 38.5 34.0 41.0 23.0 41.0 54-55 34.194195663583834 38.0 34.0 40.0 22.5 41.0 56-57 33.933765041688936 38.0 33.5 40.0 22.0 41.0 58-59 33.78271754578114 37.0 33.5 40.0 22.0 41.0 60-61 33.66117195157901 37.0 33.0 40.0 22.0 41.0 62-63 33.38342975672067 36.5 33.0 40.0 21.5 41.0 64-65 33.08638860462497 36.0 33.0 39.0 20.5 41.0 66-67 32.7850164886389 35.5 33.0 39.0 20.0 41.0 68-69 32.46097548992587 35.0 33.0 39.0 20.0 41.0 70-71 32.16275071478729 35.0 32.5 37.5 19.0 40.0 72-73 31.850133430955022 35.0 32.0 37.0 18.0 39.0 74-75 31.559470841711466 35.0 32.0 36.5 18.0 39.0 76-77 31.270491567547097 35.0 32.0 36.0 16.0 39.0 78-79 30.993513290370203 35.0 32.0 36.0 13.5 37.0 80-81 30.780331426435026 35.0 32.0 35.0 12.5 37.0 82-83 30.594392664472977 35.0 31.5 35.0 11.0 36.5 84-85 30.407387413512534 35.0 31.0 35.0 10.0 36.0 86-87 30.271049976393446 35.0 31.0 35.0 7.5 36.0 88-89 30.166174563877895 35.0 31.0 35.0 7.0 36.0 90-91 30.070178571000604 35.0 31.0 35.0 7.0 35.0 92-93 30.13086449118888 35.0 31.0 35.0 7.0 35.0 94-95 30.101873964966437 35.0 31.0 35.0 7.0 35.0 96-97 30.080279060626903 35.0 32.0 35.0 3.5 35.0 98-99 30.04286566792169 35.0 31.5 35.0 2.0 35.0 100-101 29.994835017746855 35.0 31.0 35.0 2.0 35.0 102-103 29.116834169348866 33.5 29.5 35.0 2.0 35.0 104-105 29.820709119319748 34.5 31.0 35.0 2.0 35.0 106-107 29.92557913947519 35.0 31.0 35.0 2.0 35.0 108-109 29.930473904572004 35.0 32.0 35.0 2.0 35.0 110-111 29.902740696980516 35.0 32.0 35.0 2.0 35.0 112-113 29.857494601648384 35.0 32.0 35.0 2.0 35.0 114-115 29.791677551245396 35.0 32.0 35.0 2.0 35.0 116-117 29.717726724776337 35.0 31.0 35.0 2.0 35.0 118-119 29.62658834962625 35.0 31.0 35.0 2.0 35.0 120-121 29.507730247784938 35.0 31.0 35.0 2.0 35.0 122-123 29.393070997011435 35.0 31.0 35.0 2.0 35.0 124-125 28.47765495845486 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24324.0 3 9477.0 4 5505.0 5 4548.0 6 4779.0 7 5895.0 8 6632.0 9 7549.0 10 8651.0 11 9430.0 12 10105.0 13 11642.0 14 14992.0 15 12752.0 16 6918.0 17 5816.0 18 5361.0 19 4692.0 20 4638.0 21 4895.0 22 5937.0 23 7739.0 24 10715.0 25 15244.0 26 18761.0 27 20838.0 28 24799.0 29 29651.0 30 34138.0 31 40980.0 32 50663.0 33 66140.0 34 92179.0 35 148150.0 36 296618.0 37 484499.0 38 153089.0 39 287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.26401921579496 17.51587618673971 12.393683839223689 24.82642075824164 2 17.208937812425294 19.64027539860719 37.10523493423303 26.045551854734484 3 19.219528389911 24.17138039046952 29.06120839523248 27.547882824387 4 13.479900077393857 15.418942971336696 35.167565798268804 35.93359115300064 5 14.3579896656994 37.04269041805854 33.32440378804496 15.274916128197107 6 33.8002991521625 34.872740478307875 16.540801430560983 14.786158938968644 7 29.696841497198278 31.421077578638982 20.33944212507573 18.54263879908701 8 28.36227263440013 33.17487071235679 18.929292796142253 19.53356385710083 9 27.8847599925463 14.080908632814628 18.653731734981296 39.380599639657774 10-11 26.601906436207006 24.683995780518664 26.53593584614772 22.17816193712661 12-13 26.951787741313694 22.392550825707094 26.969283630229178 23.68637780275003 14-15 24.380836998730366 23.353477288918366 24.555134341969108 27.710551370382152 16-17 24.220217349555405 26.39131608442436 25.02069503561823 24.367771530402006 18-19 24.23420459917637 25.152715687878196 27.175692995180807 23.437386717764632 20-21 25.280936748447196 24.1420594527984 27.059361417370724 23.517642381383677 22-23 25.037502415524067 23.76318426741087 26.963510232085053 24.23580308498001 24-25 24.52393828582728 24.314629027353117 26.940372628005676 24.221060058813922 26-27 24.20070352171309 24.350743413509402 27.074291018069204 24.374262046708306 28-29 24.362754086415446 24.432683653499296 27.060824941186056 24.1437373188992 30-31 24.59010499065152 24.399359988494176 26.92827436598111 24.082260654873195 32-33 24.26169920039884 24.41426099211904 26.835175739679006 24.48886406780311 34-35 24.418762131282424 24.493849488501088 26.976945844217404 24.110442535999084 36-37 24.643877972926816 24.323919439600676 26.74396725810608 24.288235329366433 38-39 24.612320062994804 24.55236353372577 26.6295980904999 24.20571831277952 40-41 24.627858155722393 24.402618152882177 26.779412500490597 24.190111190904833 42-43 24.5688634989571 24.53126395003185 26.478411267760922 24.421461283250135 44-45 24.501227997688122 24.60837255625164 26.38338875827067 24.507010687789567 46-47 24.620421424725617 24.658862416305453 26.13142504620859 24.589291112760343 48-49 24.842603157405232 24.93647795425454 25.586260541064004 24.634658347276222 50-51 24.68374664016853 25.231147117099056 25.424939469851477 24.660166772880935 52-53 24.90140569914311 25.086099596873506 25.509271549768776 24.50322315421461 54-55 24.805029079722875 24.998284669378638 25.69196736886859 24.5047188820299 56-57 24.641739199740638 25.142571723322316 25.487925936113363 24.727763140823676 58-59 24.686320339912886 24.883170674633362 25.655620975916243 24.774888009537506 60-61 24.82575061618753 25.03078690428464 25.777492930248115 24.36596954927972 62-63 24.673090251091498 25.063635075204 25.740691300894202 24.522583372810303 64-65 24.681892215482744 24.80890162478562 25.81206322725546 24.697142932476176 66-67 24.663609685068977 24.99635229490834 25.678285893193397 24.661752126829285 68-69 24.714098482083624 25.00852475835531 25.61271887953812 24.66465788002294 70-71 24.520255799655562 24.85363787042952 25.852076274893548 24.774030055021374 72-73 24.83522950117767 25.103901967957654 25.442656390776268 24.618212140088406 74-75 24.64304836828318 25.231543279289838 25.422854312678034 24.70255403974895 76-77 24.69889156141354 24.94187558498928 25.423828852959613 24.935404000637572 78-79 24.58449550334597 25.039554733562913 25.516009478752515 24.859940284338602 80-81 24.61795916288254 25.249587628711527 25.308226001938632 24.8242272064673 82-83 24.791089438766704 25.095991601633656 25.340943953367972 24.771975006231663 84-85 24.564534153860905 25.17714347348016 25.230833300875755 25.027489071783176 86-87 24.404178733496682 25.2560325161745 25.407096344686792 24.932692405642033 88-89 24.45054163047357 25.05693764320555 25.376885099671213 25.115635626649663 90-91 24.481835518224404 25.017534556033432 25.41565361724184 25.08497630850033 92-93 24.493021784304467 25.223589317086383 25.315625546764707 24.96776335184444 94-95 24.454667989282854 24.96119483652398 25.395938497748087 25.18819867644508 96-97 24.47509374690119 24.997775598813295 25.409312287052906 25.11781836723261 98-99 24.357986515335167 24.963945627549794 25.302889682308106 25.375178174806933 100-101 24.331962639222823 25.051195050520942 25.418690874772487 25.19815143548375 102-103 24.40318373905994 24.846598013997763 25.50942760882239 25.240790638119908 104-105 24.324916199644516 25.077284464047704 25.32818373196109 25.269615604346683 106-107 24.380041497331074 24.997049171880796 25.360435416408283 25.26247391437985 108-109 24.390611493384988 24.904771107585503 25.26606112087395 25.438556278155556 110-111 24.34229486352195 25.06939688668161 25.312802759458673 25.275505490337764 112-113 24.27635783178336 24.98469801842709 25.437570761366086 25.301373388423464 114-115 24.0871602371487 25.179224623201385 25.389261202100666 25.34435393754924 116-117 24.054608064779966 25.180072514352176 25.2538032285724 25.511516192295453 118-119 24.08704911215586 24.833031133566706 25.4120742488648 25.667845505412636 120-121 23.8592692276812 24.936038548233824 25.59027282629927 25.614419397785703 122-123 23.87143218347555 24.96848446212084 25.47354192704932 25.686541427354285 124-125 23.868648659014852 25.002547008792874 25.36587032189397 25.762934010298306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 35.0 1 34.5 2 69.0 3 132.0 4 114.5 5 72.0 6 137.5 7 306.5 8 492.5 9 570.5 10 527.5 11 480.0 12 528.0 13 622.5 14 739.0 15 889.0 16 1037.5 17 1207.0 18 1398.5 19 1574.0 20 1768.0 21 1967.5 22 2192.0 23 2530.5 24 2873.0 25 3374.0 26 4262.0 27 5303.5 28 6119.5 29 7346.0 30 9295.0 31 11009.0 32 13136.5 33 15603.5 34 18145.0 35 20794.5 36 23783.0 37 26991.0 38 29342.5 39 31928.0 40 34534.5 41 37010.0 42 39504.0 43 43715.5 44 48515.0 45 51848.0 46 55419.0 47 59346.5 48 64439.5 49 68867.5 50 69489.5 51 69535.5 52 69916.5 53 70299.0 54 71059.0 55 70245.5 56 66946.5 57 61565.0 58 57710.5 59 53682.0 60 47512.5 61 41869.0 62 36126.0 63 28581.5 64 21762.0 65 17197.0 66 13310.5 67 10210.5 68 8184.0 69 6572.5 70 5525.0 71 4578.0 72 3651.0 73 3318.5 74 2729.0 75 2092.0 76 1491.5 77 860.5 78 506.5 79 296.0 80 154.5 81 63.5 82 36.0 83 15.0 84 6.0 85 4.0 86 3.5 87 3.5 88 1.5 89 1.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12378462194762462 2 0.0017375382557991838 3 0.01911292081379102 4 0.05614046019599432 5 0.024565196030264323 6 0.018693515027908458 7 0.01384039093412453 8 0.0044936334201703025 9 0.003534991623867305 10-11 0.006141299007566081 12-13 0.00386452474134646 14-15 0.002456519603026432 16-17 0.008957309284206136 18-19 0.004613463644708177 20-21 0.011473743999501507 22-23 0.008118497712441015 24-25 0.012312555571266629 26-27 0.008298243049247825 28-29 0.012342513127401098 30-31 0.01845385457883271 32-33 0.011743362004711724 34-35 0.018274109242025896 36-37 0.013061494474628347 38-39 0.01911292081379102 40-41 0.008477988386054638 42-43 0.007489389033617171 44-45 0.01524839607244456 46-47 0.014169924051603687 48-49 0.01893317547698421 50-51 0.013870348490259001 52-53 0.019232751038328895 54-55 0.015188480960175624 56-57 0.018363981910429303 58-59 0.014828990286561998 60-61 0.017914618568412272 62-63 0.01998168994169061 64-65 0.015218438516310091 66-67 0.010215526641853823 68-69 0.021509525304548513 70-71 0.013301154923704095 72-73 0.016027292531940747 74-75 0.016746273879167994 76-77 0.01162353178017385 78-79 0.027201460970097565 80-81 0.019502369043539112 82-83 0.007908794819499732 84-85 0.010964465545215539 86-87 0.01180327711698066 88-89 0.018963133033118678 90-91 0.010814677764543196 92-93 0.007189813472272485 94-95 0.006800365242524391 96-97 0.002366646934623026 98-99 0.0012582173576476847 100-101 0.009915951080509135 102-103 0.0028160102766400566 104-105 0.0018873260364715271 106-107 2.9957556134468683E-4 108-109 0.017105764552781618 110-111 2.9957556134468683E-4 112-113 0.007579261702020577 114-115 0.001977198704874933 116-117 0.007010068135465672 118-119 0.009226927289416355 120-121 0.0032354160625226182 122-123 5.991511226893737E-4 124-125 0.024205705356650696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1669028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.43156719892166 #Duplication Level Percentage of deduplicated Percentage of total 1 78.98526516608148 44.57262298944373 2 10.811022351883937 12.201658686787649 3 3.6622215620249468 6.199947065242519 4 1.8576088378941809 4.19311111859745 5 1.1116155190514807 3.1365102931358906 6 0.7305156500079094 2.473448579597117 7 0.5058228023462522 1.998106142294458 8 0.376801751213819 1.7010810675435188 9 0.27602504057373195 1.4018873063149473 >10 1.577394466542947 15.97892300841054 >50 0.07888200718512378 3.0226539626031816 >100 0.026079799907906757 2.54044220030496 >500 3.193051227040098E-4 0.10276772734796757 >1k 4.2574016360534637E-4 0.47683985237610443 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 0.1653057947499982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2745 0.16446698317823308 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1983022453787474E-4 2 0.0 0.0 0.0 0.0 1.1983022453787474E-4 3 0.0 0.0 0.0 0.0 1.1983022453787474E-4 4 0.0 0.0 0.0 0.0 1.797453368068121E-4 5 0.0 0.0 0.0 0.0 2.3966044907574948E-4 6 5.991511226893737E-5 0.0 0.0 0.0 2.3966044907574948E-4 7 5.991511226893737E-5 0.0 0.0 0.0 2.3966044907574948E-4 8 5.991511226893737E-5 0.0 0.0 0.0 2.3966044907574948E-4 9 5.991511226893737E-5 0.0 0.0 0.0 2.3966044907574948E-4 10-11 5.991511226893737E-5 0.0 0.0 0.0 2.3966044907574948E-4 12-13 5.991511226893737E-5 0.0 0.0 5.991511226893737E-5 2.6961800521021817E-4 14-15 5.991511226893737E-5 0.0 0.0 5.991511226893737E-5 3.2953311747915554E-4 16-17 5.991511226893737E-5 0.0 0.0 5.991511226893737E-5 3.594906736136242E-4 18-19 5.991511226893737E-5 0.0 0.0 5.991511226893737E-5 3.594906736136242E-4 20-21 5.991511226893737E-5 0.0 0.0 5.991511226893737E-5 4.194057858825616E-4 22-23 5.991511226893737E-5 0.0 0.0 5.991511226893737E-5 4.7932089815149896E-4 24-25 5.991511226893737E-5 0.0 0.0 1.1983022453787474E-4 5.991511226893737E-4 26-27 5.991511226893737E-5 0.0 0.0 2.3966044907574945E-4 7.189813472272485E-4 28-29 5.991511226893737E-5 0.0 0.0 0.0012582173576476847 8.388115717651232E-4 30-31 5.991511226893737E-5 0.0 0.0 0.0055721054410111754 8.388115717651232E-4 32-33 5.991511226893737E-5 0.0 0.0 0.01231255557126663 8.388115717651232E-4 34-35 5.991511226893737E-5 0.0 0.0 0.021299822411607234 0.0010185569085719352 36-37 5.991511226893737E-5 0.0 0.0 0.034541062223042394 0.0010185569085719352 38-39 5.991511226893737E-5 0.0 0.0 0.05679952643095263 0.0010185569085719352 40-41 5.991511226893737E-5 0.0 0.0 0.0802562928842416 0.0010185569085719352 42-43 5.991511226893737E-5 0.0 0.0 0.09880002013147772 0.001048514464706404 44-45 5.991511226893737E-5 0.0 0.0 0.12336521616174204 0.0011383871331098099 46-47 5.991511226893737E-5 0.0 0.0 0.1523940880560422 0.001228259801513216 48-49 5.991511226893737E-5 0.0 0.0 0.185107739354882 0.0013181324699166222 50-51 5.991511226893737E-5 0.0 0.0 0.2187800324500248 0.0013181324699166222 52-53 5.991511226893737E-5 0.0 0.0 0.25098440529457866 0.001408005138320028 54-55 5.991511226893737E-5 0.0 0.0 0.2888207986924126 0.0014679202505889655 56-57 5.991511226893737E-5 0.0 0.0 0.3291736268055419 0.0015577929189923715 58-59 5.991511226893737E-5 0.0 0.0 0.37878333976422207 0.001617708031261309 60-61 5.991511226893737E-5 0.0 0.0 0.43663138065988105 0.0016476655873957776 62-63 5.991511226893737E-5 0.0 0.0 0.49495874245369165 0.0017974533680681212 64-65 5.991511226893737E-5 0.0 0.0 0.5519679717775856 0.0017974533680681212 66-67 5.991511226893737E-5 0.0 0.0 0.6183239586154337 0.0017974533680681212 68-69 5.991511226893737E-5 0.0 0.0 0.6880052341842078 0.0017974533680681212 70-71 5.991511226893737E-5 0.0 0.0 0.7718264762484512 0.0018573684803370584 72-73 5.991511226893737E-5 0.0 0.0 0.8554380154197533 0.002097028929412808 74-75 5.991511226893737E-5 0.0 0.0 0.9416259044186197 0.002097028929412808 76-77 5.991511226893737E-5 0.0 0.0 1.0396470280906014 0.002097028929412808 78-79 5.991511226893737E-5 0.0 0.0 1.152257481600069 0.002097028929412808 80-81 5.991511226893737E-5 0.0 0.0 1.2711889794539097 0.002097028929412808 82-83 5.991511226893737E-5 0.0 0.0 1.3997368528269147 0.0022168591539506828 84-85 5.991511226893737E-5 0.0 0.0 1.5317298451553838 0.0022168591539506828 86-87 5.991511226893737E-5 0.0 0.0 1.670702948063184 0.0023067318223540887 88-89 5.991511226893737E-5 0.0 0.0 1.8327733267506598 0.0024265620468919636 90-91 5.991511226893737E-5 0.0 0.0 2.0115001066488998 0.002456519603026432 92-93 5.991511226893737E-5 0.0 0.0 2.2048461739407608 0.0025164347152953695 94-95 5.991511226893737E-5 0.0 0.0 2.401787147968758 0.0025164347152953695 96-97 5.991511226893737E-5 0.0 0.0 2.6073259406073475 0.002666222495967713 98-99 5.991511226893737E-5 0.0 0.0 2.8344341736627543 0.0028759253889089936 100-101 5.991511226893737E-5 0.0 0.0 3.089372976367083 0.0028759253889089936 102-103 5.991511226893737E-5 0.0 0.0 3.3760368310178137 0.003025713169581337 104-105 5.991511226893737E-5 0.0 0.0 3.671598079840482 0.003175500950253681 106-107 5.991511226893737E-5 0.0 0.0 3.9738398636811363 0.003175500950253681 108-109 5.991511226893737E-5 0.0 0.0 4.289802208231378 0.003235416062522618 110-111 5.991511226893737E-5 0.0 0.0 4.6480646220434885 0.0034451189554638986 112-113 5.991511226893737E-5 0.0 0.0 5.021066153473758 0.003534991623867305 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2495 0.0 82.17936 1 GTATCAA 4950 0.0 59.60392 1 TCAACGC 6680 0.0 43.58119 4 CAACGCA 6850 0.0 42.56483 5 ATCAACG 7040 0.0 41.26804 3 TATCAAC 7130 0.0 40.997623 2 AACGCAG 7105 0.0 40.946056 6 ACGCAGA 8440 0.0 34.4694 7 GTACATG 8380 0.0 33.713913 1 CGCAGAG 8895 0.0 32.638348 8 TACATGG 8730 0.0 31.98345 2 ACATGGG 8760 0.0 31.126343 3 GCAGAGT 9570 0.0 30.14797 9 CATGGGG 7095 0.0 29.70418 4 GTATAGG 635 0.0 29.097977 1 AGAGTAC 8880 0.0 26.730139 10-11 GCGTAAT 90 0.009449441 26.490635 1 CAGAGTA 9330 0.0 26.237926 10-11 ATGGGGG 3710 0.0 25.982834 5 GAGTACT 5465 0.0 24.873526 12-13 >>END_MODULE