FastQCFastQC Report
Mon 15 Aug 2016
SRR2921493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752490
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.071.902261
GTATCAA41150.056.4050981
TCAACGC51150.044.435464
CAACGCA52500.043.4061665
AACGCAG55700.040.8033036
TATCAAC56750.040.781062
ATCAACG56900.040.1507573
GTACATG93800.036.8000761
TACATGG97250.035.2377052
ACATGGG97100.034.8632473
ACGCAGA66300.034.0982747
CATGGGG82700.033.167094
CGCAGAG70750.031.952678
GCAGAGT77650.028.2705929
ATGGGGG45350.027.4208535
CAGAGTA74050.025.46664610-11
AGAGTAC69000.025.30443210-11
ATGGGGA32650.022.2325155
GAGTACT27550.021.80970412-13
GTGGTAT9650.021.5856111
Produced by FastQC (version 0.11.5)