##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921493_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752490
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.971530222711685	31.0	30.0	34.0	26.0	34.0
2	29.98268178420419	31.0	30.0	34.0	25.0	34.0
3	30.121174443220788	31.0	30.0	34.0	26.0	34.0
4	33.515973842932056	37.0	35.0	37.0	27.0	37.0
5	33.52327317131624	37.0	35.0	37.0	27.0	37.0
6	33.53087720899977	37.0	35.0	37.0	27.0	37.0
7	33.3542314078825	37.0	35.0	37.0	27.0	37.0
8	33.283535426735675	37.0	35.0	37.0	26.0	37.0
9	34.75732415020913	39.0	35.0	39.0	27.0	39.0
10-11	34.66555415437463	39.0	35.0	39.0	26.5	39.0
12-13	34.54171864033461	39.0	35.0	39.0	25.5	39.0
14-15	35.70146448767183	40.0	36.0	41.0	25.5	41.0
16-17	35.63532288344013	40.0	36.0	41.0	25.0	41.0
18-19	35.65264224046928	40.0	36.0	41.0	25.0	41.0
20-21	35.677429257798906	40.0	36.0	41.0	25.0	41.0
22-23	35.640892958019734	40.0	36.0	41.0	25.0	41.0
24-25	35.606747256760386	40.0	36.0	41.0	25.0	41.0
26-27	35.529234403619995	40.0	35.0	41.0	25.0	41.0
28-29	35.489108069090264	40.0	35.0	41.0	25.0	41.0
30-31	35.40031669224931	40.0	35.0	41.0	25.0	41.0
32-33	35.30042368287408	40.0	35.0	41.0	25.0	41.0
34-35	35.143559164388954	40.0	35.0	41.0	24.0	41.0
36-37	34.9986621892279	40.0	35.0	41.0	24.0	41.0
38-39	34.873960479089746	39.5	35.0	41.0	23.5	41.0
40-41	34.729149381736846	39.0	34.0	41.0	23.0	41.0
42-43	34.53638423043783	39.0	34.0	41.0	21.5	41.0
44-45	34.33692488972833	39.0	34.0	41.0	20.5	41.0
46-47	34.094481851536955	39.0	33.0	41.0	18.0	41.0
48-49	33.94418826926259	39.0	33.0	41.0	17.5	41.0
50-51	33.74734292349743	38.0	33.0	40.5	15.0	41.0
52-53	33.55950219402108	38.0	33.0	40.0	14.5	41.0
54-55	33.35832187344864	38.0	33.0	40.0	13.0	41.0
56-57	33.12141952307859	37.0	33.0	40.0	12.0	41.0
58-59	32.881140833899195	37.0	32.0	40.0	9.5	41.0
60-61	32.57624266044314	36.5	32.0	40.0	8.0	41.0
62-63	32.25955497606263	36.0	32.0	39.5	7.0	41.0
64-65	31.952259927303437	35.0	31.0	39.0	6.5	41.0
66-67	31.90792500955783	35.0	32.0	39.0	2.0	41.0
68-69	31.65956724432094	35.0	32.0	38.5	2.0	40.5
70-71	31.35560802058785	35.0	31.0	37.5	2.0	40.0
72-73	31.027728831548252	35.0	31.0	37.0	2.0	39.0
74-75	30.718722503409435	35.0	31.0	36.5	2.0	39.0
76-77	30.434592494108383	35.0	31.0	36.0	2.0	39.0
78-79	30.164409212035448	35.0	31.0	35.5	2.0	37.0
80-81	29.924142505806024	35.0	30.5	35.0	2.0	37.0
82-83	29.73142129199025	35.0	30.0	35.0	2.0	36.5
84-85	29.533289776261206	35.0	30.0	35.0	2.0	36.0
86-87	29.383655826852078	35.0	30.0	35.0	2.0	36.0
88-89	29.261044285559404	35.0	30.0	35.0	2.0	35.5
90-91	29.15616265998665	34.0	30.0	35.0	2.0	35.0
92-93	29.056174357628286	34.0	30.0	35.0	2.0	35.0
94-95	28.990118060588077	34.0	30.0	35.0	2.0	35.0
96-97	28.89143418792689	34.0	30.0	35.0	2.0	35.0
98-99	28.833084639569982	34.0	30.0	35.0	2.0	35.0
100-101	28.771626942236473	34.0	29.5	35.0	2.0	35.0
102-103	27.79328155938122	33.5	28.0	34.5	2.0	35.0
104-105	28.612304207156676	34.0	29.5	35.0	2.0	35.0
106-107	28.934323448350632	35.0	30.0	35.0	2.0	35.0
108-109	28.97571940496094	35.0	30.0	35.0	2.0	35.0
110-111	28.918781562234308	35.0	30.0	35.0	2.0	35.0
112-113	28.849718115367274	35.0	30.0	35.0	2.0	35.0
114-115	28.7328292886122	35.0	30.0	35.0	2.0	35.0
116-117	28.66988599079025	35.0	30.0	35.0	2.0	35.0
118-119	28.515691672991004	35.0	30.0	35.0	2.0	35.0
120-121	28.35206277924553	35.0	30.0	35.0	2.0	35.0
122-123	28.230443255025705	35.0	30.0	35.0	2.0	35.0
124-125	27.39329582479786	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65266.0
3	11784.0
4	7628.0
5	6605.0
6	6815.0
7	7949.0
8	8694.0
9	9061.0
10	9545.0
11	10229.0
12	10335.0
13	10734.0
14	12198.0
15	10640.0
16	7586.0
17	6727.0
18	6262.0
19	5594.0
20	5445.0
21	5839.0
22	6892.0
23	9397.0
24	13361.0
25	18197.0
26	21931.0
27	23042.0
28	27419.0
29	32254.0
30	36605.0
31	43883.0
32	55151.0
33	71200.0
34	101193.0
35	159175.0
36	307263.0
37	465010.0
38	135413.0
39	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.831996666419315	17.05954208931028	12.247141625805567	24.86131961846484
2	16.725473901459544	19.328421759171533	38.35063114565093	25.595473193717993
3	19.17136122098366	24.077441596319698	28.792600583323345	27.958596599373298
4	13.30994240168588	15.079274024935488	35.615233444123476	35.99555012925516
5	14.382644065319925	37.176464141567216	33.40924956519407	15.031642227918793
6	33.734811140067194	34.4359605981635	17.32561890055061	14.503609361218706
7	29.984604573874357	31.093680340263465	20.55509159336667	18.366623492495513
8	27.89081047325318	33.54476659856734	19.359900630931932	19.204522297247543
9	27.86944570016941	14.683077850381512	18.721660570692748	38.72581587875633
10-11	26.42724570953653	24.83458479878325	26.889320869386278	21.848848622293943
12-13	26.677704471481135	22.459868322660856	27.014289970990802	23.84813723486721
14-15	24.164874318736544	23.637787032533968	24.34336705687337	27.85397159185612
16-17	24.279114344761883	26.661531159390716	24.782780314928942	24.276574180918463
18-19	24.362773376964988	25.85466651556961	26.310783619975297	23.471776487490107
20-21	25.494942874132963	24.79897250233308	25.92287658237695	23.783208041157003
22-23	25.26782592064491	24.481240267590415	25.82087656271633	24.43005724904835
24-25	24.722559736160534	24.907243937310717	25.82615418347258	24.54404214305617
26-27	24.375684173418605	25.006286902548013	25.964036919317845	24.653992004715533
28-29	24.38679626327434	25.06704429362075	26.020150824327253	24.526008618777652
30-31	24.861444765158296	24.96077979137361	25.783576380642685	24.394199062825408
32-33	24.453297798118413	25.008821000489867	25.787838095531722	24.750043105859998
34-35	24.677178775685416	25.073195263671344	25.912521884370605	24.337104076272635
36-37	24.752033500847084	24.974687392508837	25.748952028077227	24.524327078566852
38-39	24.765405312816707	25.153061078835805	25.670068658378177	24.41146494996931
40-41	24.751441143266554	24.97694313496294	25.832252208278206	24.4393635134923
42-43	24.62679558192427	25.026370457322965	25.676960684538535	24.669873276214233
44-45	24.573883627488062	25.043513013938153	25.719053090587284	24.663550267986498
46-47	24.650188537900572	25.048875966956192	25.591935922236964	24.708999572906272
48-49	24.72901094346495	25.238961909388863	25.41040134411257	24.621625803033616
50-51	24.73066867472803	25.41070154740805	25.26043948797861	24.598190289885313
52-53	24.76806161804463	25.26668132131652	25.40214167240006	24.56311538823879
54-55	24.643600915231477	25.144346481793562	25.57447444978786	24.637578153187096
56-57	24.59345671682689	25.261828374397734	25.34086497842121	24.803849930354165
58-59	24.703930298527315	25.154978109823663	25.387609400930184	24.753482190718838
60-61	24.796721747533688	25.242578238938208	25.539482929764045	24.421217083764056
62-63	24.61091085122519	25.328021697141406	25.482238776447456	24.57882867518595
64-65	24.632913719168293	25.080547195747382	25.551471427723303	24.73506765736102
66-67	24.596868677326267	25.31269987462037	25.44230353742937	24.64812791062399
68-69	24.577527152904164	25.28704009443049	25.412584015824415	24.722848736840934
70-71	24.53860276906846	25.112865915064546	25.621190704004714	24.72734061186228
72-73	24.716545069105695	25.355945418658255	25.33434097013963	24.593168542096425
74-75	24.59120408770224	25.50492610134428	25.32987668049442	24.57399313045906
76-77	24.572160005479642	25.20585750653561	25.40597851524596	24.81600397273879
78-79	24.480937479041	25.296294235080403	25.428633808665573	24.79413447721302
80-81	24.574602940865915	25.494401190820682	25.213818633855173	24.717177234458234
82-83	24.754222981649335	25.416904938825407	25.25074221064058	24.578129868884684
84-85	24.56734746406702	25.45292721575427	25.18865725118716	24.791068068991553
86-87	24.37935792055405	25.581419902722153	25.337811519534753	24.701410657189044
88-89	24.50113977903012	25.385766973689766	25.34826898556813	24.764824261711986
90-91	24.488804795780155	25.3376081192017	25.374474152544913	24.799112932473243
92-93	24.55183289011962	25.498205219886604	25.230907257851648	24.719054632142132
94-95	24.531776158828148	25.31797274735359	25.348961767937894	24.80128932588037
96-97	24.504002307822027	25.34249956414464	25.324979748062766	24.82851837997057
98-99	24.373759667637295	25.344910482065742	25.266328159664216	25.015001690632754
100-101	24.391049812574742	25.381234989358266	25.39986765953404	24.82784753853296
102-103	24.42237858729882	25.255160052283166	25.470638368778925	24.85182299163909
104-105	24.33292189312712	25.432055015433285	25.33823980059131	24.89678329084828
106-107	24.282610277385842	25.412897307461296	25.385562468524274	24.918929946628584
108-109	24.364810436851993	25.390347566917438	25.314901819229817	24.929940177000745
110-111	24.29033981058989	25.57213356859326	25.334029906618856	24.803496714197998
112-113	24.285246343200452	25.568407488906335	25.38799282335969	24.758353344533518
114-115	24.179053118053083	25.75644783595072	25.348453207019254	24.716045838976942
116-117	24.144380426399554	25.748436189721296	25.289914433049404	24.817268950829746
118-119	24.378999449461258	25.441210327248807	25.264065549859506	24.91572467343043
120-121	24.139014141127234	25.577550573971486	25.456061947460107	24.827373337441173
122-123	24.151409392295626	25.651142408425677	25.369660643666574	24.827787555612126
124-125	24.088800199266334	25.719622797734687	25.323258552346072	24.868318450652907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	137.5
2	164.0
3	175.5
4	165.0
5	161.0
6	162.0
7	199.0
8	258.0
9	304.5
10	330.5
11	360.0
12	440.0
13	518.5
14	576.5
15	683.5
16	785.0
17	869.5
18	953.0
19	1007.0
20	1140.0
21	1346.5
22	1584.5
23	1894.5
24	2282.0
25	2842.5
26	3825.0
27	5103.0
28	6170.0
29	7573.5
30	9633.5
31	11506.5
32	13886.5
33	16527.0
34	19043.5
35	21850.0
36	25339.0
37	29079.5
38	31836.0
39	34956.5
40	37971.0
41	40681.5
42	43654.0
43	47954.0
44	52509.5
45	56379.0
46	60519.5
47	64363.0
48	69057.0
49	73119.5
50	73682.0
51	73775.0
52	73689.0
53	73193.5
54	73969.0
55	73127.0
56	69024.5
57	63018.0
58	58594.0
59	54745.5
60	49034.5
61	43034.0
62	36920.0
63	29281.5
64	22369.0
65	17532.0
66	13597.0
67	10595.5
68	8488.0
69	6849.5
70	5776.5
71	4743.0
72	3752.0
73	3258.0
74	2640.0
75	2018.5
76	1449.0
77	904.0
78	531.0
79	338.0
80	197.0
81	93.5
82	62.0
83	38.5
84	26.0
85	24.5
86	22.5
87	15.0
88	12.5
89	14.5
90	11.0
91	6.5
92	5.5
93	9.5
94	8.5
95	4.5
96	5.5
97	7.0
98	6.5
99	4.0
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0353782332566805
2	0.02499300994584848
3	0.026248366609795206
4	0.030185621601264485
5	0.03189747159755548
6	0.04536402490171128
7	0.038288378250375184
8	0.036348281587912055
9	0.02932969660311899
10-11	0.03483614742452168
12-13	0.03606297325519689
14-15	0.03412287659273377
16-17	0.036205627421554476
18-19	0.04887331739410781
20-21	0.059943280703456225
22-23	0.05321000405137833
24-25	0.06402318986128308
26-27	0.04750383739707502
28-29	0.048473885728306576
30-31	0.04804592322923383
32-33	0.05637692654451666
34-35	0.03817425491728912
36-37	0.05092753738965701
38-39	0.0607706748683302
40-41	0.04807445406250535
42-43	0.05726138237593367
44-45	0.05720432070939064
46-47	0.06379494319511096
48-49	0.04855947822812113
50-51	0.050413982390769704
52-53	0.06020005820289988
54-55	0.04576345656751251
56-57	0.04393748323813545
58-59	0.045135778235539145
60-61	0.033152828261502204
62-63	0.04214004074202991
64-65	0.04119852324406986
66-67	0.03483614742452168
68-69	0.029301165769847475
70-71	0.04873066322775023
72-73	0.030585053267065718
74-75	0.039886104913580106
76-77	0.03138391659866818
78-79	0.03181187909774093
80-81	0.024022961614616915
82-83	0.016662006630565652
84-85	0.017375277462353563
86-87	0.01669053746383717
88-89	0.02259641995104109
90-91	0.011383802475335094
92-93	0.01857357245975726
94-95	0.014636317468287979
96-97	0.010756124143361731
98-99	0.010699062476818698
100-101	0.01092730914299083
102-103	0.00624825248646212
104-105	0.006219721653190602
106-107	0.008416595815097376
108-109	0.013552145803970352
110-111	0.005820289987389372
112-113	0.007874509982938562
114-115	0.007903040816210077
116-117	0.00681886915189245
118-119	0.23980165364709644
120-121	0.35583655256235414
122-123	0.029187042436761408
124-125	0.0035092924923965326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1752490.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.25856516254311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57240039994835	47.74604839402855
2	10.064678660296432	11.928368324624664
3	3.4319418532623285	6.1011584983681395
4	1.6720978679920495	3.963444818742251
5	0.98912168652865	2.9306965957421274
6	0.6576677986437103	2.338347006074075
7	0.46216156098729455	1.9170921684171764
8	0.3412286486476402	1.617657608897017
9	0.2574094740506021	1.37283444823352
>10	1.4640600893424827	15.371124137045866
>50	0.06773204327076042	2.7148896041707284
>100	0.01911194870848662	1.7731469765860135
>500	1.9398416066919544E-4	0.0831104624744861
>1k	1.9398416066919544E-4	0.14208095659538703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.7061666543033056E-5	0.0
5	0.0	0.0	0.0	5.7061666543033056E-5	0.0
6	0.0	0.0	0.0	5.7061666543033056E-5	0.0
7	0.0	0.0	0.0	5.7061666543033056E-5	0.0
8	0.0	0.0	0.0	5.7061666543033056E-5	0.0
9	0.0	0.0	0.0	5.7061666543033056E-5	5.7061666543033056E-5
10-11	0.0	0.0	0.0	5.7061666543033056E-5	1.1412333308606611E-4
12-13	0.0	0.0	0.0	5.7061666543033056E-5	2.853083327151653E-4
14-15	0.0	0.0	0.0	5.7061666543033056E-5	2.853083327151653E-4
16-17	0.0	0.0	0.0	5.7061666543033056E-5	2.853083327151653E-4
18-19	0.0	0.0	0.0	5.7061666543033056E-5	3.9943166580123137E-4
20-21	0.0	0.0	0.0	5.7061666543033056E-5	3.9943166580123137E-4
22-23	0.0	0.0	0.0	1.1412333308606611E-4	3.9943166580123137E-4
24-25	0.0	0.0	0.0	2.2824666617213222E-4	3.9943166580123137E-4
26-27	0.0	0.0	0.0	3.4236999925819835E-4	3.9943166580123137E-4
28-29	0.0	0.0	0.0	0.0013694799970327934	4.279624990727479E-4
30-31	0.0	0.0	0.0	0.0040513783245553475	4.5649333234426445E-4
32-33	0.0	0.0	0.0	0.010099914978116851	4.5649333234426445E-4
34-35	0.0	0.0	0.0	0.01865916495957181	4.5649333234426445E-4
36-37	0.0	0.0	0.0	0.03081329993323785	4.5649333234426445E-4
38-39	0.0	0.0	0.0	0.04964364989243876	4.5649333234426445E-4
40-41	0.0	0.0	0.0	0.07241125484310895	4.5649333234426445E-4
42-43	0.0	0.0	0.0	0.09435146562890516	4.5649333234426445E-4
44-45	0.0	0.0	0.0	0.11823177307716448	4.85024165615781E-4
46-47	0.0	0.0	0.0	0.1455357805180058	5.135549988872975E-4
48-49	0.0	0.0	0.0	0.17569287128599878	6.276783319733636E-4
50-51	0.0	0.0	0.0	0.21167025204138112	6.847399985163967E-4
52-53	0.0	0.0	0.0	0.24813265696237924	7.132708317879133E-4
54-55	0.0	0.0	0.0	0.28847525520830364	9.129866646885289E-4
56-57	0.0	0.0	0.0	0.33386781094328644	9.415174979600454E-4
58-59	0.0	0.0	0.0	0.38419620083424155	9.70048331231562E-4
60-61	0.0	0.0	0.0	0.4344389982253822	9.70048331231562E-4
62-63	0.0	0.0	0.0	0.491044171436071	9.70048331231562E-4
64-65	0.0	0.0	0.0	0.5489047013107065	9.70048331231562E-4
66-67	0.0	0.0	0.0	0.6109877945095264	9.70048331231562E-4
68-69	0.0	0.0	0.0	0.6841408510176948	9.70048331231562E-4
70-71	0.0	0.0	0.0	0.7680785625024964	0.001027109997774595
72-73	0.0	0.0	0.0	0.8540990248161188	0.0012553566639467272
74-75	0.0	0.0	0.0	0.9434575946225086	0.00139801083030431
76-77	0.0	0.0	0.0	1.0445993985700346	0.001512134163390376
78-79	0.0	0.0	0.0	1.1554416858298764	0.0015406649966618925
80-81	0.0	0.0	0.0	1.2775821830652387	0.0015977266632049257
82-83	0.0	0.0	0.0	1.4092519786132873	0.001683319163019475
84-85	0.0	0.0	0.0	1.5445737208200903	0.0017118499962909916
86-87	0.0	0.0	0.0	1.6883120588419906	0.0017689116628340248
88-89	0.0	0.0	0.0	1.8516510793214227	0.0018259733293770578
90-91	0.0	0.0	0.0	2.030824712266546	0.0018259733293770578
92-93	0.0	0.0	0.0	2.2250340943457596	0.0018830349959200908
94-95	0.0	0.0	0.0	2.419614377257502	0.001997158329006157
96-97	0.0	0.0	0.0	2.6286883234711755	0.0021398124953637392
98-99	0.0	0.0	0.0	2.866892250455067	0.002225404995178289
100-101	0.0	0.0	0.0	3.122585578234398	0.002225404995178289
102-103	0.0	0.0	0.0	3.409234860113324	0.002282466661721322
104-105	0.0	0.0	0.0	3.7055275636380234	0.002282466661721322
106-107	0.0	0.0	0.0	4.008097050482457	0.002282466661721322
108-109	0.0	0.0	0.0	4.325103138962277	0.0023109974949928388
110-111	0.0	0.0	0.0	4.676346227367917	0.002368059161535872
112-113	0.0	0.0	0.0	5.058773516539324	0.0023965899948073886
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2210	0.0	71.90226	1
GTATCAA	4115	0.0	56.405098	1
TCAACGC	5115	0.0	44.43546	4
CAACGCA	5250	0.0	43.406166	5
AACGCAG	5570	0.0	40.803303	6
TATCAAC	5675	0.0	40.78106	2
ATCAACG	5690	0.0	40.150757	3
GTACATG	9380	0.0	36.800076	1
TACATGG	9725	0.0	35.237705	2
ACATGGG	9710	0.0	34.863247	3
ACGCAGA	6630	0.0	34.098274	7
CATGGGG	8270	0.0	33.16709	4
CGCAGAG	7075	0.0	31.95267	8
GCAGAGT	7765	0.0	28.270592	9
ATGGGGG	4535	0.0	27.420853	5
CAGAGTA	7405	0.0	25.466646	10-11
AGAGTAC	6900	0.0	25.304432	10-11
ATGGGGA	3265	0.0	22.232515	5
GAGTACT	2755	0.0	21.809704	12-13
GTGGTAT	965	0.0	21.585611	1
>>END_MODULE