FastQCFastQC Report
Mon 15 Aug 2016
SRR2921493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752490
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.16924490296663605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26390.15058573800706423No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.078.1712341
GTATCAA50400.061.9708631
TCAACGC67000.046.2029154
CAACGCA68300.045.2143975
AACGCAG71800.043.0870746
ATCAACG72550.042.7504963
TATCAAC72700.042.744182
ACGCAGA85250.036.0079737
CGCAGAG89650.034.107028
GTACATG86800.033.1676141
TACATGG90300.031.116812
GCAGAGT95450.031.0996539
ACATGGG90350.030.7042583
CATGGGG79200.028.0365474
AGAGTAC89400.027.28206610-11
CAGAGTA92500.026.14265810-11
ATGGGGG41400.025.5828975
GAGTACT50150.024.9696312-13
TACCGTA1751.7537335E-523.7959657
AGTACTT53050.023.71679112-13
Produced by FastQC (version 0.11.5)