##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752490
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33251031389623	31.0	31.0	34.0	28.0	34.0
2	31.39760626308852	33.0	31.0	34.0	28.0	34.0
3	31.483542274135658	33.0	31.0	34.0	28.0	34.0
4	34.991242175418975	37.0	35.0	37.0	32.0	37.0
5	34.65936524602137	37.0	35.0	37.0	32.0	37.0
6	34.63797739216771	37.0	35.0	37.0	31.0	37.0
7	34.48709664534462	37.0	35.0	37.0	30.0	37.0
8	34.460065963286524	37.0	35.0	37.0	30.0	37.0
9	36.049527244092694	39.0	37.0	39.0	30.0	39.0
10-11	35.91704831411306	39.0	37.0	39.0	30.0	39.0
12-13	35.82817163008063	39.0	37.0	39.0	30.0	39.0
14-15	37.01657527289742	40.0	37.0	41.0	30.0	41.0
16-17	36.99926247795993	40.0	37.0	41.0	30.0	41.0
18-19	37.014129324561054	40.0	37.0	41.0	30.0	41.0
20-21	36.99959999771754	40.0	37.0	41.0	30.0	41.0
22-23	36.9367083407038	40.0	37.0	41.0	30.0	41.0
24-25	36.86675073752204	40.0	37.0	41.0	30.0	41.0
26-27	36.742884124873754	40.0	36.5	41.0	30.0	41.0
28-29	36.55843942048171	40.0	36.0	41.0	29.5	41.0
30-31	36.50854384333149	40.0	36.0	41.0	30.0	41.0
32-33	36.388213912775534	40.0	36.0	41.0	30.0	41.0
34-35	36.2639230466365	40.0	35.0	41.0	29.0	41.0
36-37	36.13137050710703	40.0	35.0	41.0	28.5	41.0
38-39	35.96125141940895	40.0	35.0	41.0	27.5	41.0
40-41	35.761366398667036	40.0	35.0	41.0	27.0	41.0
42-43	35.59190665852587	39.5	35.0	41.0	26.5	41.0
44-45	35.37335790789106	39.0	35.0	41.0	26.0	41.0
46-47	35.097199413406074	39.0	35.0	41.0	24.5	41.0
48-49	34.83506781779069	39.0	34.5	41.0	24.0	41.0
50-51	34.61702891314644	39.0	34.0	41.0	23.0	41.0
52-53	34.41796586571107	38.5	34.0	41.0	23.0	41.0
54-55	34.20278974487729	38.0	34.0	40.5	22.5	41.0
56-57	33.94402764067127	38.0	33.5	40.0	22.0	41.0
58-59	33.7956447683011	37.0	33.5	40.0	21.5	41.0
60-61	33.67898276167054	37.0	33.0	40.0	22.0	41.0
62-63	33.407397759758965	36.5	33.0	40.0	21.0	41.0
64-65	33.11025398147778	36.0	33.0	39.5	20.0	41.0
66-67	32.8066776415272	35.5	33.0	39.0	20.0	41.0
68-69	32.48988211059692	35.0	33.0	39.0	19.5	41.0
70-71	32.18802332680929	35.0	32.5	37.5	18.5	40.0
72-73	31.865194951183746	35.0	32.0	37.0	18.0	39.5
74-75	31.57778617852313	35.0	32.0	36.5	17.5	39.0
76-77	31.291799667901103	35.0	32.0	36.0	16.0	39.0
78-79	31.015834897774027	35.0	32.0	36.0	13.5	37.5
80-81	30.793967725921405	35.0	32.0	35.0	12.0	37.0
82-83	30.601977472054045	35.0	31.5	35.0	10.5	36.5
84-85	30.411526741950027	35.0	31.0	35.0	9.0	36.0
86-87	30.275080028987325	35.0	31.0	35.0	7.0	36.0
88-89	30.169014088525472	35.0	31.0	35.0	7.0	36.0
90-91	30.0706312161553	35.0	31.0	35.0	7.0	35.0
92-93	30.129274061478238	35.0	31.0	35.0	7.0	35.0
94-95	30.101617983554828	35.0	31.0	35.0	6.0	35.0
96-97	30.078165353297308	35.0	32.0	35.0	2.0	35.0
98-99	30.041082117444322	35.0	32.0	35.0	2.0	35.0
100-101	29.99681995332356	35.0	31.5	35.0	2.0	35.0
102-103	29.11863405782629	33.5	29.5	35.0	2.0	35.0
104-105	29.822019241193956	34.5	31.0	35.0	2.0	35.0
106-107	29.925153638537168	35.0	31.0	35.0	2.0	35.0
108-109	29.928454085330017	35.0	32.0	35.0	2.0	35.0
110-111	29.89760426593019	35.0	32.0	35.0	2.0	35.0
112-113	29.85197889859571	35.0	32.0	35.0	2.0	35.0
114-115	29.791396527226976	35.0	32.0	35.0	2.0	35.0
116-117	29.717788689236457	35.0	31.5	35.0	2.0	35.0
118-119	29.626254643393118	35.0	31.0	35.0	2.0	35.0
120-121	29.503797739216772	35.0	31.0	35.0	2.0	35.0
122-123	29.389200794298397	35.0	31.0	35.0	2.0	35.0
124-125	28.47844295830504	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26734.0
3	10254.0
4	5914.0
5	4940.0
6	5206.0
7	6152.0
8	7049.0
9	7990.0
10	9029.0
11	9935.0
12	10454.0
13	12027.0
14	15207.0
15	13026.0
16	7213.0
17	5998.0
18	5612.0
19	5100.0
20	4828.0
21	5069.0
22	6109.0
23	8065.0
24	11227.0
25	15559.0
26	19576.0
27	22153.0
28	25828.0
29	31066.0
30	35492.0
31	43024.0
32	53607.0
33	68765.0
34	96100.0
35	154179.0
36	308449.0
37	510002.0
38	165224.0
39	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01150827859117	17.52131845054982	12.54776549732959	24.919407773529418
2	17.032447512384014	19.742693275342504	37.480377594708244	25.744481617565242
3	19.2407240733205	24.171486003140156	29.09243138259834	27.495358540941005
4	13.39271644327107	15.505820313659752	35.38275670700653	35.71870653606265
5	14.386527263615825	37.15304770091128	33.24155471158796	15.21887032388493
6	33.732996526537235	34.85242634444012	16.665715450003482	14.748861679019164
7	29.667949622304253	31.540277273650787	20.248270178586512	18.54350292545845
8	28.066731530277135	33.363653742449614	19.099346893822457	19.47026783345079
9	27.916161411537793	14.208563610788541	18.660876561773325	39.21439841590034
10-11	26.52003930708144	24.697321777081825	26.70025382970127	22.082385086135464
12-13	26.877179507225225	22.386460192090517	27.1223856612891	23.613974639395163
14-15	24.299603523324105	23.36887053221915	24.69804888737734	27.633477057079403
16-17	24.117044325192367	26.42802174614602	25.15013748990262	24.304796438759
18-19	24.13644145172335	25.28737731111618	27.27291143574526	23.3032698014152
20-21	25.16405265778574	24.295458858073086	27.116105937626177	23.424382546514995
22-23	25.00451540759793	23.90598022579606	26.96738377711777	24.122120589488244
24-25	24.499971179895095	24.379269156381316	26.969226405607877	24.151533258115712
26-27	24.13627707613933	24.496826220220314	27.09448636013712	24.27241034350324
28-29	24.290123333491227	24.56171839375976	27.085400923731385	24.06275734901763
30-31	24.548154392437276	24.43726012980279	26.95095853907225	24.063626938687687
32-33	24.25056091831095	24.559333500926375	26.8504026655854	24.33970291517728
34-35	24.409891927786724	24.594640022304464	26.93050201313783	24.064966036770976
36-37	24.515536420481087	24.50081225461507	26.78728117421228	24.196370150691564
38-39	24.584373268162498	24.70360073808025	26.61192514409744	24.10010084965981
40-41	24.56956165067589	24.567478688875415	26.68861861112102	24.174341049327673
42-43	24.496513501159313	24.637611273212652	26.504068009982134	24.361807215645904
44-45	24.458804910663073	24.73009444768167	26.41541684763099	24.39568379402427
46-47	24.63427468164362	24.76425391350203	26.123314478973043	24.478156925881304
48-49	24.74909630723756	25.067768039396928	25.649550129800158	24.533585523565357
50-51	24.710416777835388	25.251303135200477	25.536142365969045	24.502137720995094
52-53	24.83330108651792	25.139759980960108	25.596694501661137	24.43024443086083
54-55	24.622577411402535	25.06850749680184	25.729369776686823	24.579545315108803
56-57	24.600091489193517	25.134586995921225	25.524514737721944	24.740806777163314
58-59	24.633443804799374	24.987515601930614	25.7529590163607	24.626081576909314
60-61	24.73434605768893	25.064114509212597	25.85551884168974	24.346020591408728
62-63	24.595179451442224	25.16078530748897	25.807807586709348	24.43622765435946
64-65	24.63044009895071	24.875591083250033	25.890209683849115	24.603759133950142
66-67	24.607128610487642	25.115120719104304	25.74164336859574	24.53610730181231
68-69	24.619392191846654	25.079648826014544	25.647920797504202	24.653038184634596
70-71	24.55177270641626	24.888000246542134	25.866293124898696	24.693933922142907
72-73	24.73216866689438	25.20552307688357	25.542504161255536	24.519804094966517
74-75	24.53016211008784	25.338301258408485	25.469994837839977	24.661541793663698
76-77	24.58419317725264	25.018661656744218	25.51057037327879	24.88657479272435
78-79	24.47806107043643	25.117824035361195	25.560666606924638	24.843448287277745
80-81	24.60813709629781	25.275617447862192	25.316710723033324	24.799534732806674
82-83	24.69293687764898	25.193157441504926	25.39428999267831	24.71961568816779
84-85	24.486180196180037	25.196117260601692	25.352856983064235	24.96484556015404
86-87	24.326462083335	25.269606346485173	25.478077952287226	24.925853617892603
88-89	24.428886841218368	25.116679646132855	25.43206783300178	25.02236567964699
90-91	24.381768516555464	25.06356730801332	25.500513189556557	25.054150985874664
92-93	24.41300421432872	25.214753859805803	25.413771752869707	24.95847017299577
94-95	24.41363261025008	25.02620755930441	25.48193661451405	25.078223215931455
96-97	24.341841909672045	25.051791851111087	25.491805032675728	25.114561206541136
98-99	24.30743306860162	25.06613537723414	25.35030636828332	25.276125185880925
100-101	24.251943384958143	25.103115037147095	25.500509190684994	25.14443238720977
102-103	24.292106613909613	24.912243560461995	25.592896286339784	25.202753539288608
104-105	24.224954278165647	25.07998824499485	25.429813774469384	25.265243702370118
106-107	24.232846500825968	25.039187211302803	25.378105946698543	25.349860341172676
108-109	24.28383495658179	24.89579914904303	25.430800533060903	25.389565361314276
110-111	24.187116490026565	25.124701815512356	25.420339912484046	25.26784178197703
112-113	24.172224262361315	25.00528581492714	25.506389630044314	25.316100292667226
114-115	23.946081123685587	25.217873859273958	25.522076980908132	25.313968036132323
116-117	23.942144093643204	25.162445819293882	25.411853259059885	25.483556828003024
118-119	23.9806152688998	24.876519924876177	25.518045639262983	25.62481916696104
120-121	23.833585745899104	24.96735946022134	25.672383706664263	25.52667108721529
122-123	23.82885833659538	25.003252537264377	25.507966433815433	25.65992269232481
124-125	23.702287799837276	25.100589051310905	25.42769074820447	25.769432400647354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	36.5
2	74.5
3	103.5
4	76.0
5	68.5
6	140.5
7	294.5
8	506.0
9	611.0
10	516.5
11	440.5
12	506.0
13	616.5
14	723.0
15	837.0
16	1015.0
17	1221.5
18	1383.0
19	1527.5
20	1764.5
21	1957.0
22	2192.0
23	2606.5
24	2993.5
25	3565.5
26	4538.5
27	5640.0
28	6541.0
29	7887.0
30	9810.0
31	11692.0
32	13926.0
33	16359.5
34	18812.0
35	21524.5
36	25054.0
37	28708.0
38	31355.5
39	34226.0
40	37157.0
41	39784.0
42	42808.0
43	47587.5
44	52332.0
45	55889.5
46	59846.0
47	63729.5
48	68740.5
49	72920.5
50	73709.0
51	74069.5
52	73872.5
53	73535.0
54	74381.0
55	73599.0
56	69483.0
57	63362.5
58	59026.0
59	55123.5
60	49237.5
61	42917.5
62	36521.0
63	29004.5
64	21892.0
65	17077.5
66	13298.5
67	10239.5
68	8192.5
69	6638.5
70	5533.0
71	4470.0
72	3573.5
73	3195.0
74	2615.0
75	1978.5
76	1410.0
77	826.5
78	470.5
79	285.0
80	151.5
81	63.0
82	37.0
83	16.0
84	9.5
85	6.0
86	3.5
87	2.0
88	1.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11475101141803948
2	0.0016547883297479585
3	0.01980039829043247
4	0.05923000987166831
5	0.02556362661127881
6	0.019857459956975503
7	0.016319636631307456
8	0.00542085832158814
9	0.004279624990727479
10-11	0.007646263316766429
12-13	0.004964364989243876
14-15	0.003081329993323785
16-17	0.010242569144474434
18-19	0.0051355499888729745
20-21	0.013266837471255184
22-23	0.008930150813984673
24-25	0.013751861636870968
26-27	0.009301051646514388
28-29	0.01420835496921523
30-31	0.021141347454193746
32-33	0.013095652471626086
34-35	0.02136959412036588
36-37	0.015321057466804375
38-39	0.021512248286723462
40-41	0.010128445811388368
42-43	0.009072804980342257
44-45	0.017289684962539017
46-47	0.016376698297850486
48-49	0.021312532453822846
50-51	0.01560636579951954
52-53	0.02162637161980953
54-55	0.017660585795068733
56-57	0.021141347454193746
58-59	0.017289684962539017
60-61	0.02005717578987612
62-63	0.022054334118882277
64-65	0.017375277462353563
66-67	0.01135527164206358
68-69	0.024194146614246015
70-71	0.014493663301930396
72-73	0.0179458941277839
74-75	0.01806001746086996
76-77	0.013352429971069736
78-79	0.029272634936575958
80-81	0.021597840786538012
82-83	0.009301051646514388
84-85	0.012667689972553338
86-87	0.01215413497366604
88-89	0.020741915788392514
90-91	0.012182665806937557
92-93	0.007703324983309462
94-95	0.007589201650223395
96-97	0.0024251208280789047
98-99	0.0013694799970327934
100-101	0.011383802475335094
102-103	0.003109860826595301
104-105	0.0025677749944364872
106-107	2.853083327151653E-4
108-109	0.019686274957346404
110-111	5.420858321588141E-4
112-113	0.009044274147070739
114-115	0.0024251208280789047
116-117	0.0077889174831240115
118-119	0.01044228497737505
120-121	0.003880193324926248
122-123	6.847399985163967E-4
124-125	0.024850355779490896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1752490.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.73068742899198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9043531342128	46.129332350070335
2	10.37247316632036	11.976200124608948
3	3.5841978192053965	6.207546119526643
4	1.717502508429616	3.9661040189063925
5	0.9966176206264667	2.8767710171306127
6	0.6694797876921108	2.3189717017968694
7	0.48083158366755957	1.9431116503889359
8	0.3512651932940068	1.6223024862992603
9	0.27503469202977426	1.429014765392997
>10	1.551081870666694	16.029521620489405
>50	0.07372166304916238	2.880458962361453
>100	0.02294443943146258	2.144662159309511
>500	1.9860854982672922E-4	0.07510104312555846
>1k	2.979128247400938E-4	0.4009019805931529
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2966	0.16924490296663605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2639	0.15058573800706423	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	5.7061666543033056E-5
14-15	0.0	0.0	0.0	0.0	5.7061666543033056E-5
16-17	0.0	0.0	0.0	0.0	1.7118499962909918E-4
18-19	0.0	0.0	0.0	0.0	1.7118499962909918E-4
20-21	0.0	0.0	0.0	2.8530833271516528E-5	1.7118499962909918E-4
22-23	0.0	0.0	0.0	8.559249981454959E-5	2.2824666617213222E-4
24-25	0.0	0.0	0.0	1.7118499962909918E-4	2.853083327151653E-4
26-27	0.0	0.0	0.0	2.567774994436488E-4	2.853083327151653E-4
28-29	0.0	0.0	0.0	0.0012268258306752107	2.853083327151653E-4
30-31	0.0	0.0	0.0	0.0040513783245553475	2.853083327151653E-4
32-33	0.0	0.0	0.0	0.010328161644288982	2.853083327151653E-4
34-35	0.0	0.0	0.0	0.019087127458644555	3.4236999925819835E-4
36-37	0.0	0.0	0.0	0.031412447431939695	3.4236999925819835E-4
38-39	0.0	0.0	0.0	0.05072782155675638	3.4236999925819835E-4
40-41	0.0	0.0	0.0	0.0745795981717442	3.4236999925819835E-4
42-43	0.0	0.0	0.0	0.09657687062408345	3.4236999925819835E-4
44-45	0.0	0.0	0.0	0.12088514057141553	3.4236999925819835E-4
46-47	0.0	0.0	0.0	0.14887388801077325	3.7090083252971483E-4
48-49	0.0	0.0	0.0	0.17991543461018322	3.9943166580123137E-4
50-51	0.0	0.0	0.0	0.21657755536408196	3.9943166580123137E-4
52-53	0.0	0.0	0.0	0.25355351528396736	5.42085832158814E-4
54-55	0.0	0.0	0.0	0.29383905186334874	5.706166654303305E-4
56-57	0.0	0.0	0.0	0.3392316075983315	5.991474987018471E-4
58-59	0.0	0.0	0.0	0.39064416915360434	6.276783319733636E-4
60-61	0.0	0.0	0.0	0.44197113820906253	6.276783319733636E-4
62-63	0.0	0.0	0.0	0.49897574308555254	6.847399985163967E-4
64-65	0.0	0.0	0.0	0.557492482125433	6.847399985163967E-4
66-67	0.0	0.0	0.0	0.620517092822213	6.847399985163967E-4
68-69	0.0	0.0	0.0	0.6941266426627256	6.847399985163967E-4
70-71	0.0	0.0	0.0	0.7786349708129576	7.988633316024627E-4
72-73	0.0	0.0	0.0	0.8653687039583677	9.129866646885289E-4
74-75	0.0	0.0	0.0	0.9558399762623467	9.129866646885289E-4
76-77	0.0	0.0	0.0	1.0585224452065347	9.129866646885289E-4
78-79	0.0	0.0	0.0	1.170277719131065	9.129866646885289E-4
80-81	0.0	0.0	0.0	1.2947006830281484	9.415174979600454E-4
82-83	0.0	0.0	0.0	1.4273119960741574	9.70048331231562E-4
84-85	0.0	0.0	0.0	1.5641744032776221	9.70048331231562E-4
86-87	0.0	0.0	0.0	1.7095104679627273	0.0010271099977745952
88-89	0.0	0.0	0.0	1.8761305342683838	0.0010841716643176281
90-91	0.0	0.0	0.0	2.056759239710355	0.0010841716643176281
92-93	0.0	0.0	0.0	2.2534222734509184	0.0012553566639467272
94-95	0.0	0.0	0.0	2.450884170523084	0.0012553566639467272
96-97	0.0	0.0	0.0	2.6634388783958824	0.0014265416635758264
98-99	0.0	0.0	0.0	2.905380344538343	0.0015406649966618925
100-101	0.0	0.0	0.0	3.165838321474017	0.0015406649966618925
102-103	0.0	0.0	0.0	3.4572807833425583	0.0015977266632049257
104-105	0.0	0.0	0.0	3.757853111857985	0.0017118499962909916
106-107	0.0	0.0	0.0	4.065615210357834	0.0017689116628340248
108-109	0.0	0.0	0.0	4.3891548596568315	0.0017689116628340248
110-111	0.0	0.0	0.0	4.749014259710469	0.0017689116628340248
112-113	0.0	0.0	0.0	5.141940895525795	0.0017689116628340248
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2440	0.0	78.171234	1
GTATCAA	5040	0.0	61.970863	1
TCAACGC	6700	0.0	46.202915	4
CAACGCA	6830	0.0	45.214397	5
AACGCAG	7180	0.0	43.087074	6
ATCAACG	7255	0.0	42.750496	3
TATCAAC	7270	0.0	42.74418	2
ACGCAGA	8525	0.0	36.007973	7
CGCAGAG	8965	0.0	34.10702	8
GTACATG	8680	0.0	33.167614	1
TACATGG	9030	0.0	31.11681	2
GCAGAGT	9545	0.0	31.099653	9
ACATGGG	9035	0.0	30.704258	3
CATGGGG	7920	0.0	28.036547	4
AGAGTAC	8940	0.0	27.282066	10-11
CAGAGTA	9250	0.0	26.142658	10-11
ATGGGGG	4140	0.0	25.582897	5
GAGTACT	5015	0.0	24.96963	12-13
TACCGTA	175	1.7537335E-5	23.795965	7
AGTACTT	5305	0.0	23.716791	12-13
>>END_MODULE