FastQCFastQC Report
Mon 15 Aug 2016
SRR2921492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981460
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.10310579067959989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.068.548691
GTATCAA45200.061.7459451
TCAACGC57000.047.7034264
CAACGCA58350.046.497785
AACGCAG61600.044.140046
ATCAACG62500.043.3107533
TATCAAC64850.042.382382
ACGCAGA71100.038.3259667
GTACATG102450.037.522741
TACATGG105100.036.2268562
CGCAGAG76700.035.4456758
ACATGGG108500.034.599033
GCAGAGT82950.032.346299
CATGGGG92900.031.446624
AGAGTAC76000.027.47627410-11
CAGAGTA80500.025.97728710-11
ATGGGGG50250.025.5756055
GTGGTAT8850.025.551381
ATGGGGA36950.025.1199265
GTATACG1701.3980034E-524.503221
Produced by FastQC (version 0.11.5)