##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921492_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1981460
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.551899104700574	31.0	30.0	34.0	25.0	34.0
2	29.63094132609288	31.0	30.0	34.0	24.0	34.0
3	29.774016129520657	31.0	30.0	34.0	25.0	34.0
4	33.110536170298666	37.0	35.0	37.0	26.0	37.0
5	33.13903384373139	37.0	35.0	37.0	26.0	37.0
6	33.15633724627295	37.0	35.0	37.0	26.0	37.0
7	32.996607552007106	37.0	35.0	37.0	25.0	37.0
8	32.94104549170813	37.0	35.0	37.0	25.0	37.0
9	34.36780505283983	39.0	35.0	39.0	25.0	39.0
10-11	34.246173528610214	39.0	35.0	39.0	24.5	39.0
12-13	34.08339027787591	39.0	35.0	39.0	22.5	39.0
14-15	35.19676223592704	40.0	35.0	41.0	22.5	41.0
16-17	35.11200907411707	40.0	35.0	41.0	22.0	41.0
18-19	35.12172968417228	40.0	35.0	41.0	22.0	41.0
20-21	35.13863792355132	40.0	35.0	41.0	22.0	41.0
22-23	35.09643697071856	40.0	35.0	41.0	22.0	41.0
24-25	35.05338967226187	40.0	35.0	41.0	23.0	41.0
26-27	34.96962467069737	40.0	35.0	41.0	23.0	41.0
28-29	34.91265909985566	40.0	35.0	41.0	23.0	41.0
30-31	34.80682855066466	40.0	34.0	41.0	22.0	41.0
32-33	34.684461457713	39.0	34.0	41.0	21.5	41.0
34-35	34.51158312557407	39.0	34.0	41.0	19.5	41.0
36-37	34.36441184782938	39.0	34.0	41.0	18.0	41.0
38-39	34.233786450395165	39.0	33.5	41.0	17.0	41.0
40-41	34.06863121132902	39.0	33.0	41.0	15.5	41.0
42-43	33.872606058159136	39.0	33.0	41.0	15.0	41.0
44-45	33.67096232071301	38.5	33.0	41.0	12.0	41.0
46-47	33.40677076499147	38.0	33.0	41.0	9.0	41.0
48-49	33.2480996336035	38.0	33.0	40.0	7.5	41.0
50-51	33.046031713988675	38.0	32.0	40.0	6.5	41.0
52-53	32.8507348117045	38.0	32.0	40.0	2.0	41.0
54-55	32.64226328061127	37.5	32.0	40.0	2.0	41.0
56-57	32.394747307540904	37.0	32.0	40.0	2.0	41.0
58-59	32.13946938116338	36.5	31.0	40.0	2.0	41.0
60-61	31.82236128915042	36.0	31.0	39.5	2.0	41.0
62-63	31.505753333400623	35.5	31.0	39.0	2.0	41.0
64-65	31.195264098190222	35.0	31.0	39.0	2.0	41.0
66-67	31.141263512763317	35.0	31.0	39.0	2.0	40.5
68-69	30.89377252127219	35.0	31.0	38.0	2.0	40.0
70-71	30.59171646159902	35.0	30.5	37.0	2.0	40.0
72-73	30.26994968356666	35.0	30.0	37.0	2.0	39.0
74-75	29.975445126321	35.0	30.0	36.0	2.0	39.0
76-77	29.706897186922774	35.0	30.0	36.0	2.0	38.0
78-79	29.439926872104408	35.0	30.0	35.0	2.0	37.0
80-81	29.206249684576022	35.0	30.0	35.0	2.0	37.0
82-83	29.02293687482967	34.5	29.0	35.0	2.0	36.0
84-85	28.83593814661916	34.0	29.0	35.0	2.0	36.0
86-87	28.691392205747277	34.0	29.0	35.0	2.0	36.0
88-89	28.571094041767182	34.0	29.0	35.0	2.0	35.0
90-91	28.467957465707105	34.0	29.0	35.0	2.0	35.0
92-93	28.37527429269327	34.0	29.0	35.0	2.0	35.0
94-95	28.31307646886639	34.0	29.0	35.0	2.0	35.0
96-97	28.214419922683273	34.0	29.0	35.0	2.0	35.0
98-99	28.15641345270659	34.0	29.0	35.0	2.0	35.0
100-101	28.091178222119044	34.0	29.0	35.0	2.0	35.0
102-103	27.133197995417518	32.5	26.0	34.5	2.0	35.0
104-105	27.93790714927377	34.0	28.5	35.0	2.0	35.0
106-107	28.257415239268013	34.0	29.0	35.0	2.0	35.0
108-109	28.29713670727645	34.0	29.0	35.0	2.0	35.0
110-111	28.236427936975765	34.0	29.0	35.0	2.0	35.0
112-113	28.167305673594218	34.0	29.0	35.0	2.0	35.0
114-115	28.04370312799653	34.0	29.0	35.0	2.0	35.0
116-117	27.97736265178202	34.0	29.0	35.0	2.0	35.0
118-119	27.819732924207404	34.0	29.0	35.0	2.0	35.0
120-121	27.647206605230487	34.0	29.0	35.0	2.0	35.0
122-123	27.516670283528306	34.0	29.0	35.0	2.0	35.0
124-125	26.681219656213095	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87874.0
3	15760.0
4	10080.0
5	8691.0
6	9008.0
7	10372.0
8	11580.0
9	11649.0
10	12507.0
11	13148.0
12	13252.0
13	13903.0
14	14970.0
15	12959.0
16	9005.0
17	8108.0
18	7289.0
19	6886.0
20	6584.0
21	6843.0
22	8286.0
23	11196.0
24	16388.0
25	22093.0
26	25696.0
27	27914.0
28	32569.0
29	38010.0
30	43132.0
31	51468.0
32	64346.0
33	82698.0
34	115938.0
35	181869.0
36	344844.0
37	495411.0
38	129010.0
39	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.12324046410047	16.883921368270446	12.241604056587866	24.751234111041217
2	16.723913719503578	19.141061988360775	38.335988708094554	25.799035584041096
3	19.382750639234484	23.948184775996587	28.461369539372555	28.207695045396374
4	13.511236224499326	14.966015930080104	35.31228338720938	36.21046445821118
5	14.548891274078098	37.027184762074015	33.21452263848598	15.20940132536191
6	33.9617963810021	34.200070386514874	17.137349134786138	14.700784097696888
7	30.157244326501104	30.949575181812488	20.328615599622744	18.564564892063665
8	28.07980956371815	33.20366635280694	19.3233251628831	19.393198920591807
9	27.95990157641307	14.533129108765738	18.583813015378432	38.92315629944276
10-11	26.56786625492536	24.69574419262748	26.693983698134012	22.04240585431315
12-13	26.870391799296012	22.38454633393716	26.758537856160526	23.98652401060631
14-15	24.30429549569198	23.469626793408075	24.212864373971968	28.013213336927972
16-17	24.457938687723406	26.423299069006607	24.6383817678777	24.480380475392288
18-19	24.54118011219567	25.608854262528975	26.192934233059876	23.657031392215483
20-21	25.69558018820163	24.5807227534494	25.847486836314275	23.876210222034697
22-23	25.467853865022466	24.104267425140478	25.749024449670944	24.678854260166112
24-25	24.87801626146829	24.65090416824173	25.751415536598405	24.719664033691576
26-27	24.56342106106942	24.643100867414166	25.973647595606298	24.819830475910113
28-29	24.586488603157818	24.792177607039324	25.91610756724468	24.705226222558178
30-31	24.928001801627573	24.669396543127093	25.698869160584447	24.703732494660883
32-33	24.591344842840087	24.721856953273182	25.684525793374256	25.00227241051247
34-35	24.899204516566662	24.75010975101477	25.804426342364096	24.546259390054477
36-37	25.010957559403103	24.601563951750382	25.61301737858822	24.774461110258294
38-39	24.96128489945689	24.777714848292103	25.554390392140476	24.706609860110532
40-41	24.949038819366915	24.67839038915306	25.73780731346592	24.634763478014108
42-43	24.897752034480757	24.71343047668585	25.537625584842203	24.851191903991193
44-45	24.82302417819488	24.7779280421207	25.535750176496073	24.863297603188343
46-47	24.92893060829541	24.854060916910043	25.400900052370908	24.816108422423635
48-49	25.019048883803574	25.01743307391169	25.072572586472337	24.890945455812396
50-51	24.970188674195725	25.189238483789534	25.044088949977315	24.796483892037426
52-53	25.161096140560673	25.03474424598926	25.137790225554795	24.666369387895266
54-55	24.9028757071618	24.907470617818557	25.24840288984535	24.941250785174294
56-57	24.883849450003193	25.04905929604549	25.079278762077372	24.987812491873942
58-59	24.987200240746557	24.906135098808083	25.120878199813585	24.98578646063178
60-61	25.001489348382318	24.989625047709634	25.278356688335425	24.730528915572627
62-63	24.853696127721935	25.12791253955651	25.237477925982777	24.780913406738776
64-65	24.951844306267688	24.816654927532937	25.253426438548793	24.97807432765058
66-67	24.81299672744273	25.096404629845605	25.186725594911735	24.903873047799934
68-69	24.938030589413657	24.99921749420441	25.132268721577372	24.930483194804566
70-71	24.81794229091262	24.872451040589453	25.343942888766808	24.965663779731127
72-73	25.06271541463863	25.131779360885876	25.0317426580431	24.773762566432396
74-75	24.754363594641173	25.29379050656095	25.089687802383143	24.862158096414735
76-77	24.883726154869063	24.90929693481319	25.09129626689331	25.115680643424437
78-79	24.872662834813823	25.0346457050685	25.107774016604694	24.984917443512984
80-81	24.862060018153052	25.245565980492028	24.977005796255707	24.915368205099213
82-83	25.000700376790093	25.148599763815277	24.96377600764029	24.886923851754336
84-85	24.816570780295066	25.120218668880952	24.955636515986882	25.107574034837103
86-87	24.63514670730393	25.31653428680819	25.06367029099172	24.98464871489616
88-89	24.77046621557498	25.06819884379991	25.06835028460776	25.092984656017347
90-91	24.72619311710432	25.05372411935979	25.114697682424143	25.10538508111175
92-93	24.860541056783553	25.251722698428964	24.998447792053604	24.88928845273388
94-95	24.80109976861854	25.02311795609002	25.08946850910383	25.08631376618762
96-97	24.80411705606869	25.063552964673562	25.05938886691872	25.07294111233903
98-99	24.660539160016757	25.08716807235909	25.01420834532084	25.238084422303313
100-101	24.652637922732378	25.165857568425082	25.121036655229258	25.060467853613282
102-103	24.744382109662393	24.964966652391716	25.223656979301495	25.0669942586444
104-105	24.583241539094754	25.180642229084604	25.042048701038972	25.194067530781666
106-107	24.629971255738127	25.113197881151688	25.052378152342065	25.204452710768123
108-109	24.608762301062423	25.086760141239022	25.058317503125032	25.246160054573526
110-111	24.62151571373486	25.26325830606201	25.06195352198833	25.05327245821481
112-113	24.640680610342656	25.288847288407673	25.07703982348789	24.99343227776178
114-115	24.42845357484179	25.478310749749088	25.09486972872696	24.998365946682167
116-117	24.457623752431797	25.488737344512163	24.99103494692512	25.062603956130925
118-119	24.616709667627866	25.180755805129763	25.012571457479638	25.18996306976274
120-121	24.35028560488572	25.305433634217078	25.191905818762706	25.152374942134497
122-123	24.39311036098742	25.409487855896824	25.09048475698794	25.10691702612781
124-125	24.353785683354985	25.46061191509378	25.098971124253428	25.086631277297812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	221.5
2	257.5
3	236.5
4	212.0
5	198.0
6	198.5
7	247.0
8	322.5
9	388.0
10	425.0
11	453.0
12	510.5
13	588.5
14	678.0
15	785.0
16	873.5
17	989.0
18	1111.5
19	1201.0
20	1299.5
21	1469.5
22	1719.0
23	2075.5
24	2461.0
25	3104.0
26	4298.5
27	5614.5
28	6620.5
29	8006.5
30	10134.0
31	12325.0
32	15144.0
33	17885.5
34	20551.5
35	23555.0
36	26896.5
37	30414.0
38	32780.0
39	35562.5
40	38860.0
41	41643.0
42	44582.0
43	50142.0
44	55950.0
45	60123.5
46	65122.5
47	70003.5
48	75819.5
49	81508.0
50	82797.0
51	83055.5
52	83636.0
53	84519.5
54	86779.5
55	86868.5
56	82947.5
57	76622.0
58	71949.5
59	67300.5
60	60124.5
61	53128.5
62	45978.5
63	36091.5
64	26842.0
65	20974.5
66	16320.5
67	12456.0
68	9915.0
69	8050.5
70	6699.0
71	5396.5
72	4252.0
73	3735.5
74	3033.0
75	2266.5
76	1573.5
77	893.5
78	553.0
79	366.5
80	207.5
81	107.0
82	62.0
83	41.0
84	32.5
85	24.5
86	23.0
87	19.5
88	11.5
89	9.0
90	9.5
91	8.5
92	10.0
93	10.5
94	9.5
95	7.5
96	5.0
97	7.5
98	7.0
99	8.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03441906473004754
2	0.02886760267681407
3	0.029019006187356797
4	0.03426766121950481
5	0.036235906856560314
6	0.048802398231607
7	0.04279672564674533
8	0.037598538451444896
9	0.03199660856136385
10-11	0.0386835969436678
12-13	0.037951813309377934
14-15	0.03704339224612155
16-17	0.03936491274111009
18-19	0.05453049771380699
20-21	0.06543155047288364
22-23	0.05892119951954619
24-25	0.06967084876808011
26-27	0.05215850938197087
28-29	0.052637953832022855
30-31	0.05190617019773298
32-33	0.05975391882753121
34-35	0.0424182168703885
36-37	0.055110877837554126
38-39	0.0650782756149506
40-41	0.05203233978985193
42-43	0.06182310013828188
44-45	0.062882924712081
46-47	0.06828298325477174
48-49	0.05193140411615677
50-51	0.054934240408587603
52-53	0.0643969598175083
54-55	0.05087157954235766
56-57	0.04786874324992682
58-59	0.048146316352588495
60-61	0.036740585225036086
62-63	0.04579956193917616
64-65	0.04567339234705722
66-67	0.03764900628829247
68-69	0.03250128692983961
70-71	0.05268842166887043
72-73	0.03467140391428542
74-75	0.04236774903354092
76-77	0.034494766485318906
78-79	0.03507514660906604
80-81	0.026546082181825522
82-83	0.019354415431045793
84-85	0.019303947594198217
86-87	0.017941315999313638
88-89	0.0246283043816176
90-91	0.01380295337781232
92-93	0.020742280944354163
94-95	0.015947836443834344
96-97	0.012036579088147124
98-99	0.011607602474942719
100-101	0.012213216517113643
102-103	0.006737456219151534
104-105	0.006384181361218495
106-107	0.009841228185277523
108-109	0.014105760398897783
110-111	0.006863625811270478
112-113	0.009058976714140079
114-115	0.009614122919463427
116-117	0.007847748629798229
118-119	0.24022690339446673
120-121	0.35600012112280843
122-123	0.030356403863817587
124-125	0.003734619926720701
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1981460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.570706816593564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22783184190514	44.02746615000886
2	10.725054476306637	11.91997715789661
3	3.552167251380263	5.921893346698728
4	1.780707868522244	3.9582077955060337
5	1.0468849890739975	2.908806939926194
6	0.7243995008848322	2.4153235369054635
7	0.4807513984306476	1.8700986509699817
8	0.3482352259703578	1.548134211648717
9	0.29363893598221985	1.4685950899263986
>10	1.6912189669571271	17.023204686079794
>50	0.09655760560079164	3.641617367148605
>100	0.03191036268834511	2.9182964813085857
>500	3.666150270712516E-4	0.1362957458705187
>1k	2.749612703034387E-4	0.24208284010559616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2043	0.10310579067959989	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.046783684757704E-5
2	0.0	0.0	0.0	0.0	5.046783684757704E-5
3	0.0	0.0	0.0	0.0	5.046783684757704E-5
4	0.0	0.0	0.0	0.0	5.046783684757704E-5
5	0.0	0.0	0.0	0.0	5.046783684757704E-5
6	0.0	0.0	0.0	0.0	5.046783684757704E-5
7	0.0	0.0	0.0	0.0	5.046783684757704E-5
8	0.0	0.0	0.0	0.0	5.046783684757704E-5
9	0.0	0.0	0.0	0.0	1.5140351054273113E-4
10-11	0.0	0.0	0.0	7.570175527136556E-5	1.5140351054273113E-4
12-13	0.0	0.0	0.0	1.0093567369515407E-4	2.5233918423788517E-4
14-15	0.0	0.0	0.0	1.0093567369515407E-4	3.532748579330393E-4
16-17	0.0	0.0	0.0	1.0093567369515407E-4	3.532748579330393E-4
18-19	0.0	0.0	0.0	1.0093567369515407E-4	4.037426947806163E-4
20-21	0.0	0.0	0.0	1.261695921189426E-4	4.2897661320440486E-4
22-23	0.0	0.0	0.0	1.5140351054273113E-4	5.803801237471359E-4
24-25	0.0	0.0	0.0	2.0187134739030815E-4	7.570175527136556E-4
26-27	0.0	0.0	0.0	3.532748579330393E-4	7.570175527136556E-4
28-29	0.0	0.0	0.0	0.0011859941659180604	8.074853895612326E-4
30-31	0.0	0.0	0.0	0.004163596539925106	8.074853895612326E-4
32-33	0.0	0.0	0.0	0.01110292410646695	8.579532264088096E-4
34-35	0.0	0.0	0.0	0.020313304331149758	0.0010850584922229064
36-37	0.0	0.0	0.0	0.03305643313516296	0.001110292410646695
38-39	0.0	0.0	0.0	0.052965994771532104	0.0011607602474942719
40-41	0.0	0.0	0.0	0.0755755856792466	0.0011607602474942719
42-43	0.0	0.0	0.0	0.09677207715522897	0.0011859941659180604
44-45	0.0	0.0	0.0	0.12162748680266067	0.0012616959211894261
46-47	2.523391842378852E-5	0.0	0.0	0.15258950470864918	0.001312163758037003
48-49	5.046783684757704E-5	0.0	0.0	0.18251693195926236	0.0013878655133083685
50-51	5.046783684757704E-5	0.0	0.0	0.2165322539945293	0.0014635672685797342
52-53	5.046783684757704E-5	0.0	0.0	0.2512541257456623	0.0016149707791224652
54-55	5.046783684757704E-5	0.0	0.0	0.2908209098341627	0.0017159064528176192
56-57	5.046783684757704E-5	0.0	0.0	0.332784916172923	0.0017159064528176192
58-59	5.046783684757704E-5	0.0	0.0	0.37903868864372736	0.0017159064528176192
60-61	5.046783684757704E-5	0.0	0.0	0.4287999757754383	0.001791608208088985
62-63	5.046783684757704E-5	0.0	0.0	0.4805799763810523	0.0019682456370555042
64-65	5.046783684757704E-5	0.0	0.0	0.5355394507080637	0.0020439473923268697
66-67	5.046783684757704E-5	0.0	0.0	0.5981448023174831	0.0020691813107506585
68-69	5.046783684757704E-5	0.0	0.0	0.669632493212076	0.0020691813107506585
70-71	5.046783684757704E-5	0.0	0.0	0.754342757360734	0.0021701169844458128
72-73	5.046783684757704E-5	0.0	0.0	0.8384221735487973	0.002447690087107487
74-75	5.046783684757704E-5	0.0	0.0	0.9248988119871207	0.002472924005531275
76-77	5.046783684757704E-5	0.0	0.0	1.0253550412322228	0.0024981579239550635
78-79	5.046783684757704E-5	0.0	0.0	1.1325739606149001	0.002523391842378852
80-81	5.046783684757704E-5	0.0	0.0	1.2591472954286234	0.00254862576080264
82-83	5.046783684757704E-5	0.0	0.0	1.393517911035297	0.002573859679226429
84-85	5.046783684757704E-5	0.0	0.0	1.531017532526521	0.002573859679226429
86-87	5.046783684757704E-5	0.0	0.0	1.679317271103126	0.002624327516074006
88-89	5.046783684757704E-5	0.0	0.0	1.8476275069897954	0.0026747953529215832
90-91	5.046783684757704E-5	0.0	0.0	2.0299678015200913	0.0026747953529215832
92-93	5.046783684757704E-5	0.0	0.0	2.2351195583054917	0.00272526318976916
94-95	5.046783684757704E-5	0.0	0.0	2.446075116328364	0.0028766667003118913
96-97	5.046783684757704E-5	0.0	0.0	2.664070937591473	0.002952368455583257
98-99	5.046783684757704E-5	0.0	0.0	2.899932373098624	0.002977602374007045
100-101	5.046783684757704E-5	0.0	0.0	3.1742250663652056	0.0030280702108546223
102-103	5.046783684757704E-5	0.0	0.0	3.4675946019601707	0.0030280702108546223
104-105	5.046783684757704E-5	0.0	0.0	3.7775932897964126	0.0030280702108546223
106-107	5.046783684757704E-5	0.0	0.0	4.093118205767464	0.0030533041292784106
108-109	5.046783684757704E-5	0.0	0.0	4.425776952348269	0.003103771966125988
110-111	5.046783684757704E-5	0.0	0.0	4.7967660210148075	0.003255175476668719
112-113	5.046783684757704E-5	0.0	0.0	5.195815206968598	0.0032804093950925075
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2205	0.0	68.54869	1
GTATCAA	4520	0.0	61.745945	1
TCAACGC	5700	0.0	47.703426	4
CAACGCA	5835	0.0	46.49778	5
AACGCAG	6160	0.0	44.14004	6
ATCAACG	6250	0.0	43.310753	3
TATCAAC	6485	0.0	42.38238	2
ACGCAGA	7110	0.0	38.325966	7
GTACATG	10245	0.0	37.52274	1
TACATGG	10510	0.0	36.226856	2
CGCAGAG	7670	0.0	35.445675	8
ACATGGG	10850	0.0	34.59903	3
GCAGAGT	8295	0.0	32.34629	9
CATGGGG	9290	0.0	31.44662	4
AGAGTAC	7600	0.0	27.476274	10-11
CAGAGTA	8050	0.0	25.977287	10-11
ATGGGGG	5025	0.0	25.575605	5
GTGGTAT	885	0.0	25.55138	1
ATGGGGA	3695	0.0	25.119926	5
GTATACG	170	1.3980034E-5	24.50322	1
>>END_MODULE