FastQCFastQC Report
Mon 15 Aug 2016
SRR2921492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981460
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.1622036276281126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.1435809958313567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.081.071441
GTATCAA58050.058.3161851
TCAACGC76800.043.5625424
CAACGCA78500.042.522915
ATCAACG81000.041.231293
AACGCAG82650.040.4546476
TATCAAC82700.040.3127632
GTACATG102650.034.4301341
ACGCAGA97850.034.166987
CGCAGAG103350.032.3478978
TACATGG107600.032.2011032
ACATGGG107750.031.8239653
GTGGTAT11050.031.8223821
GCAGAGT109900.030.2026969
CATGGGG88600.028.4213544
CAGAGTA106200.026.77463310-11
AGAGTAC103550.025.42045410-11
GAGTACT58250.025.12356212-13
GTATAGG5150.024.3027291
ATGGGGG49600.024.2325695
Produced by FastQC (version 0.11.5)