##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1981460
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296892695285294	31.0	31.0	34.0	28.0	34.0
2	31.366913286162728	33.0	31.0	34.0	28.0	34.0
3	31.449790558477083	33.0	31.0	34.0	28.0	34.0
4	34.9521564906685	37.0	35.0	37.0	32.0	37.0
5	34.612037083766516	37.0	35.0	37.0	31.0	37.0
6	34.58810775892524	37.0	35.0	37.0	31.0	37.0
7	34.43386694659493	37.0	35.0	37.0	30.0	37.0
8	34.404807061459735	37.0	35.0	37.0	30.0	37.0
9	35.98851452969023	39.0	37.0	39.0	30.0	39.0
10-11	35.85636777931424	39.0	37.0	39.0	30.0	39.0
12-13	35.76267752061611	39.0	37.0	39.0	30.0	39.0
14-15	36.956249179897654	40.0	37.0	41.0	30.0	41.0
16-17	36.94219489669234	40.0	37.0	41.0	30.0	41.0
18-19	36.95695320622168	40.0	37.0	41.0	30.0	41.0
20-21	36.94235992651883	40.0	37.0	41.0	30.0	41.0
22-23	36.88124060036539	40.0	37.0	41.0	30.0	41.0
24-25	36.811936148092826	40.0	37.0	41.0	30.0	41.0
26-27	36.686321954518384	40.0	36.5	41.0	30.0	41.0
28-29	36.50307021085462	40.0	36.0	41.0	29.5	41.0
30-31	36.451377267267574	40.0	36.0	41.0	30.0	41.0
32-33	36.332055151252106	40.0	35.5	41.0	29.0	41.0
34-35	36.20911196794283	40.0	35.0	41.0	29.0	41.0
36-37	36.07924106466949	40.0	35.0	41.0	28.0	41.0
38-39	35.90509523280813	40.0	35.0	41.0	27.5	41.0
40-41	35.70053924883672	40.0	35.0	41.0	27.0	41.0
42-43	35.53375112290937	39.0	35.0	41.0	26.0	41.0
44-45	35.31020081152282	39.0	35.0	41.0	25.5	41.0
46-47	35.03046137696446	39.0	35.0	41.0	24.0	41.0
48-49	34.77394168946131	39.0	34.0	41.0	24.0	41.0
50-51	34.55130585527843	39.0	34.0	41.0	23.0	41.0
52-53	34.34513086310095	38.0	34.0	41.0	23.0	41.0
54-55	34.12404641022276	38.0	34.0	40.0	22.0	41.0
56-57	33.854357393033425	37.5	33.0	40.0	21.5	41.0
58-59	33.698262392377345	37.0	33.0	40.0	21.5	41.0
60-61	33.569125291451755	37.0	33.0	40.0	22.0	41.0
62-63	33.2941149455452	36.0	33.0	40.0	21.0	41.0
64-65	32.99275357564624	36.0	33.0	39.0	20.0	41.0
66-67	32.6838707316827	35.0	33.0	39.0	20.0	41.0
68-69	32.3618483340567	35.0	33.0	38.5	19.0	40.5
70-71	32.058605523200065	35.0	32.0	37.0	18.0	40.0
72-73	31.74820763477436	35.0	32.0	37.0	18.0	39.0
74-75	31.46098937147356	35.0	32.0	36.5	17.0	39.0
76-77	31.180555751819366	35.0	32.0	36.0	14.5	38.5
78-79	30.912905635238662	35.0	32.0	35.5	13.0	37.0
80-81	30.701794131599932	35.0	31.5	35.0	11.0	37.0
82-83	30.524334076892796	35.0	31.0	35.0	10.0	36.5
84-85	30.34513288181442	35.0	31.0	35.0	7.5	36.0
86-87	30.208794777588245	35.0	31.0	35.0	7.0	36.0
88-89	30.10971455391479	35.0	31.0	35.0	7.0	35.5
90-91	30.02099588182451	35.0	31.0	35.0	7.0	35.0
92-93	30.080264552400756	35.0	31.0	35.0	7.0	35.0
94-95	30.055315272576788	35.0	31.0	35.0	3.5	35.0
96-97	30.036043624398168	35.0	31.0	35.0	2.0	35.0
98-99	29.994031168936036	35.0	31.0	35.0	2.0	35.0
100-101	29.9473216718985	35.0	31.0	35.0	2.0	35.0
102-103	29.067978157520212	33.5	29.5	35.0	2.0	35.0
104-105	29.775158721346884	34.5	31.0	35.0	2.0	35.0
106-107	29.877728795938346	35.0	31.0	35.0	2.0	35.0
108-109	29.88411751940488	35.0	31.0	35.0	2.0	35.0
110-111	29.851116348551066	35.0	31.5	35.0	2.0	35.0
112-113	29.804532768766464	35.0	31.5	35.0	2.0	35.0
114-115	29.738151665943292	35.0	31.0	35.0	2.0	35.0
116-117	29.661569751597305	35.0	31.0	35.0	2.0	35.0
118-119	29.572131155814397	35.0	31.0	35.0	2.0	35.0
120-121	29.45362031027626	35.0	31.0	35.0	2.0	35.0
122-123	29.336350468846206	35.0	31.0	35.0	2.0	35.0
124-125	28.431711969961544	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31373.0
3	11853.0
4	6647.0
5	5691.0
6	5925.0
7	6883.0
8	8329.0
9	9154.0
10	10294.0
11	11347.0
12	12004.0
13	13646.0
14	17035.0
15	14383.0
16	8075.0
17	6822.0
18	6191.0
19	5664.0
20	5445.0
21	5877.0
22	6943.0
23	9314.0
24	12893.0
25	18045.0
26	22342.0
27	25228.0
28	29544.0
29	35282.0
30	41561.0
31	49423.0
32	61275.0
33	79345.0
34	110131.0
35	175841.0
36	355580.0
37	573182.0
38	172571.0
39	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95068944836552	17.540298030862107	12.564578153397019	24.944434367375358
2	17.121751141979995	19.613440212533696	37.26586949708467	25.998939148401632
3	19.33612886714248	23.948153902768123	28.807324722604598	27.90839250748479
4	13.581632142941904	15.343763586757554	35.020999036018765	36.053605234281775
5	14.546422874220061	37.053288371059914	33.06090099549704	15.339387759222985
6	34.06801366754785	34.556645161160056	16.582577965243562	14.792763206048532
7	30.02478371038897	31.231008409299697	20.07107019190971	18.673137688401628
8	28.259036159780347	33.164088031110175	18.995884109026946	19.58099170008252
9	28.062921929002876	14.163793242675796	18.41296149746439	39.360323330856936
10-11	26.706549410793272	24.59196147191653	26.46082789753032	22.240661219759875
12-13	27.105224906579128	22.218041583642716	26.870183853885926	23.80654965589223
14-15	24.45914395708884	23.20747594120509	24.478271885074967	27.855108216631102
16-17	24.371910403856965	26.23119491471528	24.892441423225588	24.50445325820216
18-19	24.398451556896955	25.13360873548019	26.923074981893613	23.54486472572924
20-21	25.462777421650625	24.014455958287694	26.804622474371463	23.718144145690218
22-23	25.260894055512033	23.68662126803899	26.61041133337102	24.442073343077954
24-25	24.692844866530553	24.23165157874281	26.62844841292876	24.447055141797883
26-27	24.38058388066994	24.236963226954956	26.84997080142394	24.532482090951166
28-29	24.552416241853596	24.375702080416804	26.770782148184995	24.301099529544604
30-31	24.779399200559514	24.270668933859767	26.618511108345967	24.331420757234753
32-33	24.450169431982026	24.367516606908463	26.539733799857	24.642580161252507
34-35	24.654930418192507	24.40122189980028	26.664457359831218	24.279390322176
36-37	24.904051431007257	24.22840491545396	26.405056282003365	24.46248737153542
38-39	24.819670054060495	24.43095645352663	26.333939333823487	24.415434158589395
40-41	24.810700506548383	24.368530605899704	26.41655377508767	24.40421511246424
42-43	24.819099115741487	24.416906475637152	26.18030051010352	24.58369389851784
44-45	24.783371447709442	24.44159136963659	26.046509045360438	24.728528137293527
46-47	24.92185609103535	24.571145470454724	25.748257110054638	24.758741328455287
48-49	25.066550698798114	24.83315947407596	25.24688813803676	24.853401689089168
50-51	24.965543697859687	25.063417010220164	25.190717935322347	24.780321356597803
52-53	25.158990908423206	24.93852250582342	25.22327631442454	24.679210271328834
54-55	24.904850648327997	24.837337514184068	25.37148628839794	24.88632554909
56-57	24.919082007331582	24.90340824767872	25.227130697449496	24.950379047540206
58-59	24.943011918534804	24.767681448234228	25.360301971212184	24.92900466201878
60-61	25.09182716175891	24.814496892149368	25.45085729328383	24.642818652807893
62-63	24.85196213117994	24.937651328510675	25.464229744979356	24.746156795330027
64-65	24.889902919727373	24.67163951203692	25.55054849786166	24.887909070374047
66-67	24.8603631114319	24.861725914971153	25.427945548427477	24.84996542516947
68-69	24.95925562931091	24.792517321878886	25.29467575759794	24.953551291212264
70-71	24.790758890996255	24.711940933221783	25.57040805896583	24.926892116816138
72-73	25.113630656262625	24.97542387660931	25.13344298680095	24.77750248032711
74-75	24.80258322699446	25.119681710252145	25.148049960425787	24.92968510232761
76-77	24.921675584739017	24.76530436612872	25.17955181323482	25.133468235897443
78-79	24.882581112868788	24.916960545184093	25.15600109145863	25.044457250488495
80-81	24.864021451672908	25.03358133801377	25.039361215056356	25.063035995256968
82-83	24.965325271164453	24.971508174049976	25.0507502763379	25.012416278447663
84-85	24.776971445228064	25.05410251653991	24.93975219470092	25.229173843531104
86-87	24.615767834659096	25.133688489981914	25.098760013860343	25.15178366149865
88-89	24.719860294227143	24.95009516559773	25.048857394459066	25.28118714571606
90-91	24.649384323136534	24.95907138802949	25.14481800995208	25.246726278881898
92-93	24.739835759059787	25.069367457117092	25.100281558060594	25.090515225762527
94-95	24.64363720330439	24.861700825593264	25.148734229124525	25.345927741977814
96-97	24.66283454421438	24.895446850056413	25.072997314788946	25.36872129094026
98-99	24.57059814203861	24.883126688499505	25.009147484706666	25.537127684755216
100-101	24.53446590205323	24.99370337151017	25.118626461789432	25.353204264647168
102-103	24.597290489358233	24.77595285332375	25.26015814142691	25.36659851589111
104-105	24.502195919858767	24.968305270712353	25.09061765356046	25.43888115586842
106-107	24.5359344522081	24.972961788180907	25.00205656954107	25.489047190069925
108-109	24.52554368703172	24.857083869873506	25.03951173540186	25.577860707692913
110-111	24.52084878395657	25.00779102539483	25.037819592908516	25.433540597740084
112-113	24.485682906098518	25.040428654780676	25.0177159273758	25.456172511745002
114-115	24.316597086516257	25.142777257384648	25.07398779050049	25.466637865598607
116-117	24.283656106477913	25.129719549375196	25.006592915183944	25.580031428962947
118-119	24.32803887237016	24.879735197877302	25.02315453907199	25.769071390680544
120-121	24.10570243318587	25.014188390023385	25.158002769291098	25.722106407499645
122-123	24.0752409159259	25.131463169494438	25.032394230786213	25.760901683793445
124-125	24.078136330445613	25.142663442999115	24.97920845483294	25.799991771722336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	46.5
2	83.0
3	115.5
4	93.0
5	81.0
6	172.5
7	397.5
8	637.0
9	697.0
10	547.0
11	437.0
12	468.5
13	569.5
14	693.5
15	834.5
16	999.0
17	1150.5
18	1343.5
19	1516.0
20	1699.0
21	1975.0
22	2283.0
23	2610.5
24	2953.5
25	3508.5
26	4586.0
27	5940.5
28	6937.0
29	8338.5
30	10560.0
31	12686.5
32	15208.5
33	17823.0
34	20298.5
35	22936.0
36	26390.5
37	29926.5
38	32135.5
39	34923.0
40	38098.0
41	40836.0
42	44108.0
43	49691.0
44	55125.0
45	59244.5
46	64588.0
47	69720.5
48	76069.0
49	81722.5
50	82680.5
51	83214.5
52	84201.0
53	85426.5
54	87914.5
55	88209.5
56	84169.5
57	77492.5
58	72729.0
59	67764.0
60	60115.5
61	52961.0
62	45328.5
63	35466.0
64	26691.5
65	20660.5
66	15722.5
67	12061.0
68	9617.5
69	7523.5
70	6269.5
71	5180.5
72	4095.0
73	3620.0
74	2916.0
75	2231.0
76	1511.5
77	815.5
78	462.0
79	296.0
80	158.5
81	48.5
82	32.5
83	18.5
84	8.5
85	7.0
86	5.5
87	3.5
88	2.5
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1145115218071523
2	0.001665438615970042
3	0.022357251723476627
4	0.05707912347460963
5	0.0272526318976916
6	0.021045087965439625
7	0.016149707791224654
8	0.005803801237471359
9	0.004239298295196471
10-11	0.007671111200831709
12-13	0.005248655032148012
14-15	0.0032299415582449304
16-17	0.010800117085381486
18-19	0.005803801237471359
20-21	0.0136263159488458
22-23	0.009891696022125099
24-25	0.014888011870035226
26-27	0.010043099532667831
28-29	0.01486277795161144
30-31	0.02268529266298588
32-33	0.014206696072592936
34-35	0.02253388915244315
36-37	0.01617494170964844
38-39	0.022130146457662533
40-41	0.010497310064296024
42-43	0.009866462103701311
44-45	0.0182441230203991
46-47	0.017133830609752404
48-49	0.022786228336681032
50-51	0.01652821656758148
52-53	0.022735760499833457
54-55	0.018370292612518043
56-57	0.02230678388662905
58-59	0.017360935875566502
60-61	0.02162546808918676
62-63	0.02339184237885196
64-65	0.018546930041484562
66-67	0.012616959211894258
68-69	0.025814298547535655
70-71	0.015771199014867825
72-73	0.018698333552027293
74-75	0.018774035307298657
76-77	0.01397959080677884
78-79	0.031517164111311856
80-81	0.02266005874456209
82-83	0.009588889001039637
84-85	0.013500146356726857
86-87	0.013550614193574435
88-89	0.02218061429451011
90-91	0.013449678519879282
92-93	0.008251491324578846
94-95	0.007948684303493382
96-97	0.0025990935976502173
98-99	0.0020691813107506585
100-101	0.012162748680266067
102-103	0.0034822807424828156
104-105	0.0029271345371594685
106-107	2.523391842378852E-4
108-109	0.020338538249573546
110-111	6.8131579744229E-4
112-113	0.009689824674734792
114-115	0.002624327516074006
116-117	0.008402894835121577
118-119	0.010446842227448447
120-121	0.0043654678873154135
122-123	5.803801237471359E-4
124-125	0.02455260262634623
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1981460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.323413401674735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90780131229272	41.54309896590922
2	11.238222970534553	11.98520818716022
3	3.8633793453996113	6.18025721886705
4	1.8852899624791475	4.021203842052137
5	1.1526367760831606	3.073126365652799
6	0.7445596149450812	2.3821476089944995
7	0.5296348202093121	1.9769355528959975
8	0.40447733767007366	1.7254489830552098
9	0.2965611045702299	1.4232285340070092
>10	1.8319538819091736	17.863563291994975
>50	0.10698599178253086	3.8790753371963125
>100	0.03764176926958785	3.354242124384825
>500	4.7506269711447416E-4	0.1477360174885866
>1k	3.800501576915788E-4	0.44472797034115635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3214	0.1622036276281126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2845	0.1435809958313567	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0093567369515407E-4
2	0.0	0.0	0.0	0.0	1.0093567369515407E-4
3	0.0	0.0	0.0	0.0	1.0093567369515407E-4
4	0.0	0.0	0.0	0.0	1.0093567369515407E-4
5	0.0	0.0	0.0	0.0	1.0093567369515407E-4
6	0.0	0.0	0.0	0.0	2.0187134739030815E-4
7	0.0	0.0	0.0	0.0	2.0187134739030815E-4
8	0.0	0.0	0.0	0.0	2.0187134739030815E-4
9	0.0	0.0	0.0	0.0	2.5233918423788517E-4
10-11	0.0	0.0	0.0	0.0	2.5233918423788517E-4
12-13	0.0	0.0	0.0	0.0	3.532748579330393E-4
14-15	0.0	0.0	0.0	0.0	4.037426947806163E-4
16-17	0.0	0.0	0.0	0.0	4.542105316281934E-4
18-19	0.0	0.0	0.0	0.0	5.046783684757703E-4
20-21	0.0	0.0	0.0	2.523391842378852E-5	5.299122868995589E-4
22-23	0.0	0.0	0.0	7.570175527136556E-5	6.056140421709245E-4
24-25	0.0	0.0	0.0	1.5140351054273113E-4	6.056140421709245E-4
26-27	0.0	0.0	0.0	3.0280702108546225E-4	6.056140421709245E-4
28-29	0.0	0.0	0.0	0.0011859941659180604	6.560818790185015E-4
30-31	0.0	0.0	0.0	0.004289766132044048	6.8131579744229E-4
32-33	0.0	0.0	0.0	0.011557134638095143	7.065497158660785E-4
34-35	0.0	0.0	0.0	0.02094415229174447	7.570175527136556E-4
36-37	0.0	2.523391842378852E-5	0.0	0.033990088116843134	7.570175527136556E-4
38-39	0.0	5.046783684757704E-5	0.0	0.05475760297962109	7.570175527136556E-4
40-41	0.0	5.046783684757704E-5	0.0	0.07814944535847304	7.82251471137444E-4
42-43	0.0	5.046783684757704E-5	0.0	0.1000020187134739	8.579532264088097E-4
44-45	0.0	5.046783684757704E-5	0.0	0.12586678509785715	9.084210632563868E-4
46-47	0.0	5.046783684757704E-5	0.0	0.15700544043281217	9.336549816801752E-4
48-49	0.0	5.046783684757704E-5	0.0	0.1874880138887487	9.588889001039638E-4
50-51	0.0	5.046783684757704E-5	0.0	0.2228407336004764	0.0010345906553753293
52-53	0.0	5.046783684757704E-5	0.0	0.2586728977622561	0.0010850584922229064
54-55	0.0	5.046783684757704E-5	0.0	0.30003129005884555	0.0011355263290704835
56-57	0.0	5.046783684757704E-5	0.0	0.34373643676884724	0.0012364620027656373
58-59	0.0	5.046783684757704E-5	0.0	0.3910500338134507	0.0012869298396132145
60-61	0.0	5.046783684757704E-5	0.0	0.4427795665822172	0.00136263159488458
62-63	0.0	5.046783684757704E-5	0.0	0.4960483683748347	0.0014635672685797342
64-65	0.0	5.046783684757704E-5	0.0	0.5524714099704259	0.0014635672685797342
66-67	0.0	5.046783684757704E-5	0.0	0.6166664984405439	0.0014635672685797342
68-69	0.0	5.046783684757704E-5	0.0	0.6896429905221403	0.0014635672685797342
70-71	0.0	5.046783684757704E-5	0.0	0.7761700967973111	0.0015645029422748883
72-73	0.0	5.046783684757704E-5	0.0	0.863757027646281	0.0017663742896651964
74-75	0.0	5.046783684757704E-5	0.0	0.9525804204980166	0.0017663742896651964
76-77	0.0	5.046783684757704E-5	0.0	1.0558123807697355	0.001791608208088985
78-79	0.0	5.046783684757704E-5	0.0	1.1657313294237581	0.001842076044936562
80-81	0.0	5.046783684757704E-5	0.0	1.294979459590403	0.001943011718631716
82-83	0.0	5.046783684757704E-5	0.0	1.4325043150000505	0.0021701169844458128
84-85	0.0	5.046783684757704E-5	0.0	1.5727039657626194	0.0021701169844458128
86-87	0.0	5.046783684757704E-5	0.0	1.7242336458974696	0.0021953509028696016
88-89	0.0	5.046783684757704E-5	0.0	1.8963037356292833	0.00222058482129339
90-91	0.0	5.046783684757704E-5	0.0	2.0826814571073853	0.00222058482129339
92-93	0.0	5.046783684757704E-5	0.0	2.2927790619038486	0.002371988331836121
94-95	0.0	5.046783684757704E-5	0.0	2.509412251572073	0.002523391842378852
96-97	0.0	5.046783684757704E-5	0.0	2.733136172317382	0.002573859679226429
98-99	0.0	5.046783684757704E-5	0.0	2.975936935391075	0.0026495614344977944
100-101	0.0	5.046783684757704E-5	0.0	3.2587586930848973	0.0026747953529215832
102-103	0.0	5.046783684757704E-5	0.0	3.5592946615122183	0.002826198863464314
104-105	0.0	5.046783684757704E-5	0.0	3.8768887587940206	0.0029019006187356797
106-107	0.0	5.046783684757704E-5	0.0	4.200715633926499	0.003002836292430834
108-109	0.0	5.046783684757704E-5	0.0	4.541726807505577	0.0030785380477021994
110-111	0.0	5.046783684757704E-5	0.0	4.924727221341839	0.0031794737213973537
112-113	0.0	5.046783684757704E-5	0.0	5.335661582873235	0.0032299415582449304
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2970	0.0	81.07144	1
GTATCAA	5805	0.0	58.316185	1
TCAACGC	7680	0.0	43.562542	4
CAACGCA	7850	0.0	42.52291	5
ATCAACG	8100	0.0	41.23129	3
AACGCAG	8265	0.0	40.454647	6
TATCAAC	8270	0.0	40.312763	2
GTACATG	10265	0.0	34.430134	1
ACGCAGA	9785	0.0	34.16698	7
CGCAGAG	10335	0.0	32.347897	8
TACATGG	10760	0.0	32.201103	2
ACATGGG	10775	0.0	31.823965	3
GTGGTAT	1105	0.0	31.822382	1
GCAGAGT	10990	0.0	30.202696	9
CATGGGG	8860	0.0	28.421354	4
CAGAGTA	10620	0.0	26.774633	10-11
AGAGTAC	10355	0.0	25.420454	10-11
GAGTACT	5825	0.0	25.123562	12-13
GTATAGG	515	0.0	24.302729	1
ATGGGGG	4960	0.0	24.232569	5
>>END_MODULE