FastQCFastQC Report
Mon 15 Aug 2016
SRR2921491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171625
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.072.213761
GTATCAA46700.062.583071
TCAACGC60750.047.20774
CAACGCA62150.045.9517635
ATCAACG66200.043.499023
AACGCAG66650.042.936536
TATCAAC69250.042.3564152
ACGCAGA77050.036.8305137
GTACATG118600.036.0356181
CGCAGAG82000.034.533078
TACATGG125150.033.896182
ACATGGG123650.033.8262063
GCAGAGT88650.031.3386449
CATGGGG104750.031.0702954
ATGGGGG58500.026.9520685
AGAGTAC82100.026.66531410-11
CAGAGTA86950.025.14373810-11
ATGGGGA39200.022.6152975
ACCGTAT2954.780304E-922.1826138
GTATAGG5400.022.0459351
Produced by FastQC (version 0.11.5)