##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921491_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2171625
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.114874805733034	31.0	31.0	34.0	26.0	34.0
2	30.210963218787775	31.0	31.0	34.0	26.0	34.0
3	30.288729868186266	31.0	31.0	34.0	26.0	34.0
4	33.68533931963391	37.0	35.0	37.0	28.0	37.0
5	33.71961503482415	37.0	35.0	37.0	28.0	37.0
6	33.6805286363898	37.0	35.0	37.0	28.0	37.0
7	33.5847063834686	37.0	35.0	37.0	28.0	37.0
8	33.534966672422726	37.0	35.0	37.0	27.0	37.0
9	35.02331710124906	39.0	35.0	39.0	27.0	39.0
10-11	34.92441420595176	39.0	35.0	39.0	27.0	39.0
12-13	34.79089000172682	39.0	35.0	39.0	27.0	39.0
14-15	35.97190767282565	40.0	36.0	41.0	27.0	41.0
16-17	35.894041558740575	40.0	36.0	41.0	27.0	41.0
18-19	35.907542738732516	40.0	36.0	41.0	27.0	41.0
20-21	35.92820744833938	40.0	36.0	41.0	27.0	41.0
22-23	35.88726713866345	40.0	36.0	41.0	27.0	41.0
24-25	35.83693202095205	40.0	36.0	41.0	26.5	41.0
26-27	35.76388994416624	40.0	36.0	41.0	26.0	41.0
28-29	35.715895700224486	40.0	36.0	41.0	26.0	41.0
30-31	35.61841017671099	40.0	35.0	41.0	26.0	41.0
32-33	35.522146549243075	40.0	35.0	41.0	25.0	41.0
34-35	35.35998917861049	40.0	35.0	41.0	25.0	41.0
36-37	35.220533241236396	40.0	35.0	41.0	25.0	41.0
38-39	35.099184021182296	40.0	35.0	41.0	24.0	41.0
40-41	34.95624681977782	39.0	35.0	41.0	24.0	41.0
42-43	34.76273666033501	39.0	34.5	41.0	23.0	41.0
44-45	34.57295780809302	39.0	34.0	41.0	22.5	41.0
46-47	34.329946468658264	39.0	34.0	41.0	21.0	41.0
48-49	34.194121913313765	39.0	33.5	41.0	20.5	41.0
50-51	34.00016761641628	38.5	33.0	40.5	19.0	41.0
52-53	33.81015299602832	38.0	33.0	40.0	18.5	41.0
54-55	33.61073758130432	38.0	33.0	40.0	18.0	41.0
56-57	33.365373395498764	37.5	33.0	40.0	16.0	41.0
58-59	33.1229549300639	37.0	33.0	40.0	15.0	41.0
60-61	32.81240844989351	36.5	32.0	40.0	13.0	41.0
62-63	32.49994589305244	36.0	32.0	39.5	11.5	41.0
64-65	32.18790444943303	35.5	32.0	39.0	8.0	41.0
66-67	32.14340942842341	35.0	32.0	39.0	8.0	41.0
68-69	31.896752892419272	35.0	32.0	38.5	7.5	40.5
70-71	31.597790594600816	35.0	32.0	37.5	7.0	40.0
72-73	31.261169400794337	35.0	31.5	37.0	4.0	39.0
74-75	30.961874172566624	35.0	31.0	36.5	2.0	39.0
76-77	30.678483854256605	35.0	31.0	36.0	2.0	39.0
78-79	30.412452656420882	35.0	31.0	35.5	2.0	37.0
80-81	30.173005007770676	35.0	31.0	35.0	2.0	37.0
82-83	29.976805387670524	35.0	31.0	35.0	2.0	36.5
84-85	29.78818050998676	35.0	31.0	35.0	2.0	36.0
86-87	29.641162263282105	35.0	30.5	35.0	2.0	36.0
88-89	29.51639325389973	35.0	30.0	35.0	2.0	35.5
90-91	29.411055085477464	35.0	30.0	35.0	2.0	35.0
92-93	29.312374834513328	34.0	30.0	35.0	2.0	35.0
94-95	29.25159523398377	34.0	30.0	35.0	2.0	35.0
96-97	29.153280838082082	34.0	30.0	35.0	2.0	35.0
98-99	29.095010188223107	34.0	30.0	35.0	2.0	35.0
100-101	29.031141886835897	34.0	30.0	35.0	2.0	35.0
102-103	28.04655868301387	33.5	28.0	34.5	2.0	35.0
104-105	28.874047775283486	34.0	29.5	35.0	2.0	35.0
106-107	29.199129453750075	35.0	30.5	35.0	2.0	35.0
108-109	29.240088413054742	35.0	31.0	35.0	2.0	35.0
110-111	29.180819432452658	35.0	31.0	35.0	2.0	35.0
112-113	29.114404881137396	35.0	31.0	35.0	2.0	35.0
114-115	28.995592931560466	35.0	30.0	35.0	2.0	35.0
116-117	28.933702642030738	35.0	30.0	35.0	2.0	35.0
118-119	28.777026880792036	35.0	30.0	35.0	2.0	35.0
120-121	28.612015426236113	35.0	30.0	35.0	2.0	35.0
122-123	28.488278362977034	35.0	30.0	35.0	2.0	35.0
124-125	27.638306107177804	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74012.0
3	13508.0
4	9044.0
5	7590.0
6	8148.0
7	9478.0
8	10520.0
9	10906.0
10	11383.0
11	12056.0
12	12116.0
13	12700.0
14	14231.0
15	12212.0
16	8735.0
17	7939.0
18	7251.0
19	6667.0
20	6673.0
21	7187.0
22	8436.0
23	10993.0
24	16109.0
25	21543.0
26	25758.0
27	27978.0
28	33390.0
29	39143.0
30	44866.0
31	54205.0
32	68416.0
33	89098.0
34	127255.0
35	200983.0
36	388431.0
37	585367.0
38	167118.0
39	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4790730584226	17.28682656190835	12.372641348640478	24.86145903102857
2	16.601575004801845	19.332753273659964	38.46845821889131	25.597213502646888
3	19.134327945721616	24.186400003684888	28.762707102158885	27.916564948434612
4	13.479892119221617	15.183689826639919	35.5031403808993	35.83327767323917
5	14.419090577426951	37.15967592746141	33.38487944015124	15.036354054960402
6	33.561102672650215	34.46457850463716	17.401411672349383	14.572907150363237
7	29.917575887123377	31.11441159723668	20.581335996653653	18.38667651898629
8	27.969871265117575	33.233002490305495	19.55051045274178	19.24661579183515
9	27.917514414203186	14.637792043940232	18.83374505803683	38.610948483819755
10-11	26.432881965005766	24.799968307250154	26.836250517368022	21.930899210376058
12-13	26.709089246571573	22.42587200029113	27.0238498030616	23.841188950075693
14-15	24.180938133751603	23.589258137068274	24.310288239297694	27.91951548988243
16-17	24.400450701318707	26.55784238259954	24.79522894143693	24.246477974644826
18-19	24.49550595911342	25.80667676860285	26.244010133879065	23.453807138404663
20-21	25.543716407216532	24.70894509732548	25.929493455958752	23.81784503949924
22-23	25.434853622048585	24.332014247782595	25.739699222693247	24.493432907475572
24-25	24.892960653939905	24.813614225220366	25.719716343429038	24.573708777410687
26-27	24.42541352212631	24.969558561127744	25.889320779282404	24.715707137463543
28-29	24.47085675111735	25.037813223179334	25.892117941320514	24.599212084382806
30-31	24.878498705168685	24.874997293316493	25.692623032010736	24.553880969504085
32-33	24.530959441353342	24.958048975574872	25.676860542907537	24.834131040164248
34-35	24.797972864653104	25.060647297613542	25.733272586223734	24.408107251509616
36-37	24.92922127973455	24.83025777045534	25.636225251635913	24.604295698174198
38-39	24.90204747106579	25.034586049053324	25.539329262181948	24.524037217698936
40-41	24.887056588265864	24.89058103085108	25.678927426889704	24.543434953993355
42-43	24.74985831908845	24.899786672318545	25.61828167547469	24.732073333118315
44-45	24.654813580672972	24.92292714213244	25.594132556561178	24.82812672063341
46-47	24.78683814074984	24.921892297190105	25.545095117338377	24.74617444472168
48-49	24.860681114551085	25.167376160990713	25.26076219961669	24.711180524841517
50-51	24.81821723078355	25.30934457305439	25.20485331486768	24.66758488129438
52-53	24.93553904380304	25.075680572232674	25.348453167433533	24.640327216530757
54-55	24.734560537105317	25.04254011048105	25.418450256922227	24.804449095491403
56-57	24.6982319477598	25.159219603388916	25.253246066701664	24.889302382149623
58-59	24.803513265733912	25.0206161344863	25.279757489761035	24.89611311001875
60-61	24.832870803401708	25.158019863643304	25.49894615036826	24.510163182586734
62-63	24.662288772842224	25.203317764468576	25.387292498635794	24.747100964053406
64-65	24.693743906031788	25.046396505445383	25.454423164231304	24.80543642429152
66-67	24.655405796023263	25.201528156821485	25.350041377791793	24.793024669363458
68-69	24.737510018701574	25.122527568702957	25.359521681852097	24.780440730743365
70-71	24.648416821812464	25.00471081146204	25.482725719279863	24.864146647445633
72-73	24.76443585407103	25.280073222901688	25.286844590886155	24.66864633214113
74-75	24.664052377908686	25.437029615169788	25.236224598499373	24.662693408422157
76-77	24.678108227126245	25.13231741808527	25.25422448404268	24.935349870745803
78-79	24.63929039389063	25.15341026475492	25.355769246271525	24.851530095082925
80-81	24.6865449587641	25.366470985170736	25.162997542658026	24.78398651340714
82-83	24.797702637039222	25.278960567616306	25.219753268376486	24.703583526967993
84-85	24.586953523016728	25.35979287168541	25.130520268725178	24.92273333657269
86-87	24.41665902895254	25.42451489768764	25.310018270640366	24.848807802719456
88-89	24.544975523837774	25.23677025202064	25.231864886425814	24.986389337715767
90-91	24.520608636021787	25.18862715171735	25.345741606477457	24.945022605783414
92-93	24.695836123651553	25.28977090067327	25.22015495592756	24.79423801974762
94-95	24.545196844373855	25.141522588002147	25.29120274453535	25.022077823088644
96-97	24.5703277641754	25.076408306463104	25.372531510295115	24.980732419066374
98-99	24.429141468835436	25.203065947530177	25.21077986304231	25.157012720592082
100-101	24.404567684811234	25.272032659347065	25.32425740351375	24.99914225232795
102-103	24.504195380556855	25.047806914635558	25.463948693423493	24.98404901138409
104-105	24.417283351949596	25.261911357117476	25.29380192434821	25.027003366584715
106-107	24.426111280483592	25.22464624433664	25.31265341385843	25.036589061321337
108-109	24.413991617924747	25.19451480679777	25.291023810620366	25.10046976465712
110-111	24.425524096005482	25.37286420775307	25.27037749464541	24.931234201596038
112-113	24.375460523891977	25.41527741959253	25.34207714696238	24.86718490955311
114-115	24.280294587380563	25.53943366718184	25.33588248188763	24.84438926354997
116-117	24.230659325654454	25.573239307219797	25.235884627508714	24.960216739617035
118-119	24.483194845439108	25.2863561592742	25.16559806990167	25.064850925385024
120-121	24.146161336582576	25.391570427218575	25.427941844553093	25.034326391645756
122-123	24.246359070971486	25.492479770057496	25.28510774524379	24.976053413727225
124-125	24.200263913541722	25.565421293461867	25.263424258632504	24.970890534363903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161.0
1	187.5
2	221.0
3	238.0
4	231.5
5	207.5
6	202.0
7	234.5
8	308.5
9	372.5
10	396.5
11	410.5
12	458.5
13	538.0
14	623.5
15	707.0
16	780.0
17	898.5
18	1013.5
19	1114.0
20	1209.5
21	1364.0
22	1604.0
23	1879.0
24	2329.5
25	3095.0
26	4280.0
27	5675.0
28	6894.0
29	8734.5
30	11336.0
31	13732.5
32	16668.5
33	19671.0
34	22910.5
35	26608.5
36	31033.0
37	35870.0
38	39306.0
39	43177.5
40	47154.5
41	50030.5
42	53058.5
43	58737.0
44	64611.5
45	68945.5
46	74799.0
47	80052.0
48	85201.5
49	90499.5
50	91450.5
51	91525.0
52	91851.0
53	91940.5
54	92819.0
55	91571.5
56	87045.0
57	79725.5
58	74065.5
59	68512.0
60	61188.0
61	54557.0
62	47208.0
63	37338.0
64	28400.5
65	22342.5
66	17103.5
67	13251.5
68	10509.5
69	8305.5
70	6934.5
71	5670.5
72	4577.5
73	4001.0
74	3220.0
75	2432.5
76	1693.5
77	988.5
78	600.0
79	394.5
80	230.5
81	111.5
82	72.0
83	54.0
84	33.5
85	21.5
86	17.5
87	14.5
88	10.0
89	9.0
90	8.0
91	8.0
92	8.5
93	9.0
94	11.0
95	8.0
96	8.5
97	10.0
98	7.0
99	7.5
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03624014275024463
2	0.026892304150118
3	0.027398837276233236
4	0.032694410867437976
5	0.03467449490588845
6	0.04641685373855984
7	0.0414896678754389
8	0.03716111207045415
9	0.031128763023081794
10-11	0.03598687618718701
12-13	0.035388246129050824
14-15	0.03557243999309273
16-17	0.03619409428423416
18-19	0.04991653715535602
20-21	0.061244459793933115
22-23	0.05442928682438266
24-25	0.06423761008461405
26-27	0.04899556783514649
28-29	0.050100731019397916
30-31	0.049363955563230304
32-33	0.05696195245495885
34-35	0.04038450469118747
36-37	0.051942669659816955
38-39	0.06048466010476026
40-41	0.04922581016519886
42-43	0.05779082484314741
44-45	0.05875784262936742
46-47	0.06375410119150406
48-49	0.049041616301156964
50-51	0.05104472457261268
52-53	0.06073792666781788
54-55	0.04726875035975364
56-57	0.044183503137051744
58-59	0.045818223680423646
60-61	0.035664536925113684
62-63	0.04358487307891556
64-65	0.04298624302077937
66-67	0.035664536925113684
68-69	0.03154319921717608
70-71	0.05000863408737696
72-73	0.033131871294537506
74-75	0.039716801934035574
76-77	0.0326483624014275
78-79	0.03363840442065274
80-81	0.026708110286076094
82-83	0.02026132504460945
84-85	0.01890289529730041
86-87	0.017590514016001844
88-89	0.024543832383583722
90-91	0.012801473550912336
92-93	0.019662694986473266
94-95	0.015126921084441376
96-97	0.0106602198814252
98-99	0.010337880619351867
100-101	0.011074656075519483
102-103	0.005963276348356646
104-105	0.006285615610429977
106-107	0.009716226328210441
108-109	0.014965751453404707
110-111	0.005710009785299027
112-113	0.008518966211938064
114-115	0.00833477234789616
116-117	0.007897311920796637
118-119	0.24454037874863294
120-121	0.3609278765901111
122-123	0.029816381741783225
124-125	0.0037069015138433197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2171625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.603080375967735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96302519517802	42.57021331085904
2	11.264828481003782	12.301886699394801
3	3.9539441051969035	6.476925833344511
4	1.9279192607813882	4.210813214192898
5	1.0874521944986792	2.968911979061694
6	0.7485571504528307	2.4524115751308773
7	0.5239630484389257	2.0026997513563414
8	0.384154646678527	1.6780821639511279
9	0.2954132441136824	1.4517425801218302
>10	1.7170232183691874	16.89705356641941
>50	0.10008474039794979	3.727317208509074
>100	0.03295583611884511	2.9489881648549807
>500	4.2429923202717384E-4	0.13696948245289997
>1k	2.54579539216289E-4	0.1759844703505028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.604846601047603E-5
2	0.0	0.0	0.0	0.0	4.604846601047603E-5
3	0.0	0.0	0.0	0.0	4.604846601047603E-5
4	0.0	0.0	0.0	0.0	4.604846601047603E-5
5	0.0	0.0	0.0	0.0	4.604846601047603E-5
6	0.0	0.0	0.0	0.0	9.209693202095205E-5
7	0.0	0.0	0.0	0.0	9.209693202095205E-5
8	0.0	0.0	0.0	0.0	9.209693202095205E-5
9	0.0	0.0	0.0	0.0	9.209693202095205E-5
10-11	0.0	0.0	0.0	0.0	9.209693202095205E-5
12-13	0.0	0.0	0.0	0.0	2.0721809704714214E-4
14-15	0.0	0.0	0.0	0.0	3.453634950785702E-4
16-17	0.0	0.0	0.0	0.0	5.295573591204743E-4
18-19	0.0	0.0	0.0	0.0	6.446785241466643E-4
20-21	0.0	0.0	0.0	0.0	6.677027571519024E-4
22-23	0.0	0.0	0.0	9.209693202095205E-5	7.367754561676164E-4
24-25	0.0	0.0	0.0	2.3024233005238014E-4	8.058481551833304E-4
26-27	0.0	0.0	0.0	3.2233926207333216E-4	8.288723881885684E-4
28-29	0.0	0.0	0.0	0.0011742358832671387	8.288723881885684E-4
30-31	0.0	0.0	0.0	0.004029240775916652	8.288723881885684E-4
32-33	0.0	0.0	0.0	0.010591147182409485	8.288723881885684E-4
34-35	0.0	0.0	0.0	0.02033039774362517	8.749208541990445E-4
36-37	0.0	0.0	0.0	0.036171070051228923	8.749208541990445E-4
38-39	0.0	0.0	0.0	0.05797501870718932	8.979450872042825E-4
40-41	0.0	4.604846601047603E-5	0.0	0.08387728083808207	9.209693202095206E-4
42-43	0.0	4.604846601047603E-5	0.0	0.10690151384332008	9.209693202095206E-4
44-45	0.0	4.604846601047603E-5	0.0	0.1348989811776895	9.209693202095206E-4
46-47	0.0	4.604846601047603E-5	0.0	0.1691590398894837	9.900420192252347E-4
48-49	0.0	4.604846601047603E-5	0.0	0.2015771599608588	0.0011051631842514246
50-51	0.0	4.604846601047603E-5	0.0	0.23751798768203536	0.0011512116502619006
52-53	0.0	4.604846601047603E-5	0.0	0.27279111264606	0.0011972601162723766
54-55	0.0	9.209693202095205E-5	0.0	0.31239279341506937	0.0011972601162723766
56-57	0.0	1.3814539803142807E-4	0.0	0.35701375697922066	0.0012202843492776145
58-59	0.0	1.3814539803142807E-4	0.0	0.4093248143671214	0.0012433085822828526
60-61	0.0	1.3814539803142807E-4	0.0	0.4679675358314626	0.0012433085822828526
62-63	0.0	1.6116963103666608E-4	0.0	0.5303632072756577	0.0013814539803142809
64-65	0.0	1.841938640419041E-4	0.0	0.5917458124676223	0.0013814539803142809
66-67	0.0	1.841938640419041E-4	0.0	0.6633281528809072	0.001404478213319519
68-69	0.0	1.841938640419041E-4	0.0	0.7484717665342773	0.0014275024463247569
70-71	0.0	1.841938640419041E-4	0.0	0.8399700684970932	0.0014735509123352329
72-73	0.0	1.841938640419041E-4	0.0	0.9357969262648937	0.0015656478443561849
74-75	0.0	1.841938640419041E-4	0.0	1.0336959650031659	0.001680769009382375
76-77	0.0	1.841938640419041E-4	0.0	1.144764865020434	0.001703793242387613
78-79	0.0	1.841938640419041E-4	0.0	1.2706843953260807	0.001703793242387613
80-81	0.0	1.841938640419041E-4	0.0	1.406228055027917	0.001703793242387613
82-83	0.0	1.841938640419041E-4	0.0	1.5511425775628849	0.001703793242387613
84-85	0.0	1.841938640419041E-4	0.0	1.7021815460772463	0.001703793242387613
86-87	0.0	1.841938640419041E-4	0.0	1.8668508605307086	0.0017268174753928508
88-89	0.0	1.841938640419041E-4	0.0	2.050031658320382	0.001749841708398089
90-91	0.0	1.841938640419041E-4	0.0	2.252092327174351	0.001749841708398089
92-93	0.0	1.841938640419041E-4	0.0	2.4740459333448452	0.001749841708398089
94-95	0.0	1.841938640419041E-4	0.0	2.7004892649513614	0.001749841708398089
96-97	0.0	1.841938640419041E-4	0.0	2.9372474529442236	0.001795890174408565
98-99	0.0	1.841938640419041E-4	0.0	3.198572497553675	0.001795890174408565
100-101	0.0	1.841938640419041E-4	0.0	3.500374143786335	0.001795890174408565
102-103	0.0	2.3024233005238014E-4	0.0	3.8248776837621596	0.0018419386404190411
104-105	0.0	2.3024233005238014E-4	0.0	4.159143498532205	0.0018419386404190411
106-107	0.0	2.7629079606285615E-4	0.0	4.503240660795488	0.0018419386404190411
108-109	0.0	2.7629079606285615E-4	0.0	4.866401887987106	0.0018879871064295172
110-111	0.0	2.7629079606285615E-4	0.0	5.265803257928971	0.002049156737466183
112-113	0.0	2.7629079606285615E-4	0.0	5.69718528751511	0.002118229436481897
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2275	0.0	72.21376	1
GTATCAA	4670	0.0	62.58307	1
TCAACGC	6075	0.0	47.2077	4
CAACGCA	6215	0.0	45.951763	5
ATCAACG	6620	0.0	43.49902	3
AACGCAG	6665	0.0	42.93653	6
TATCAAC	6925	0.0	42.356415	2
ACGCAGA	7705	0.0	36.830513	7
GTACATG	11860	0.0	36.035618	1
CGCAGAG	8200	0.0	34.53307	8
TACATGG	12515	0.0	33.89618	2
ACATGGG	12365	0.0	33.826206	3
GCAGAGT	8865	0.0	31.338644	9
CATGGGG	10475	0.0	31.070295	4
ATGGGGG	5850	0.0	26.952068	5
AGAGTAC	8210	0.0	26.665314	10-11
CAGAGTA	8695	0.0	25.143738	10-11
ATGGGGA	3920	0.0	22.615297	5
ACCGTAT	295	4.780304E-9	22.182613	8
GTATAGG	540	0.0	22.045935	1
>>END_MODULE