FastQCFastQC Report
Mon 15 Aug 2016
SRR2921491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171625
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.12911989869337478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24990.11507511656017959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.076.851371
GTATCAA57150.058.422711
TCAACGC77850.042.441754
CAACGCA81150.040.627495
ATCAACG83200.039.855733
AACGCAG86050.038.305196
TATCAAC87300.037.9174772
GTACATG113050.035.1775171
ACGCAGA97900.033.664787
TACATGG118750.033.0894242
ACATGGG119150.032.277313
CGCAGAG104800.031.5036168
CATGGGG98400.029.9480784
GCAGAGT114100.028.5708799
AGAGTAC104950.027.20899410-11
ATGGGGG54950.025.3423525
CAGAGTA110500.024.49643310-11
GAGTACT55950.022.80793212-13
GTATAGA6450.022.1851021
ATGGGGA39350.022.0803975
Produced by FastQC (version 0.11.5)