##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2171625
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297037471939216	31.0	31.0	34.0	28.0	34.0
2	31.36496724802855	33.0	31.0	34.0	28.0	34.0
3	31.455593852529788	33.0	31.0	34.0	28.0	34.0
4	34.970482012317966	37.0	35.0	37.0	32.0	37.0
5	34.62810936510677	37.0	35.0	37.0	31.0	37.0
6	34.6031649110689	37.0	35.0	37.0	31.0	37.0
7	34.45581028032004	37.0	35.0	37.0	30.0	37.0
8	34.42474045933345	37.0	35.0	37.0	30.0	37.0
9	36.01348529327117	39.0	37.0	39.0	30.0	39.0
10-11	35.882489840557184	39.0	37.0	39.0	30.0	39.0
12-13	35.789510850169805	39.0	37.0	39.0	30.0	39.0
14-15	36.980115351407356	40.0	37.0	41.0	30.0	41.0
16-17	36.95384055718644	40.0	37.0	41.0	30.0	41.0
18-19	36.964059863005815	40.0	37.0	41.0	30.0	41.0
20-21	36.94035434294595	40.0	37.0	41.0	30.0	41.0
22-23	36.87701628964485	40.0	37.0	41.0	30.0	41.0
24-25	36.80525021585218	40.0	37.0	41.0	30.0	41.0
26-27	36.67741483911817	40.0	36.5	41.0	30.0	41.0
28-29	36.497675012951134	40.0	36.0	41.0	29.5	41.0
30-31	36.455686409946466	40.0	36.0	41.0	30.0	41.0
32-33	36.34509687446037	40.0	36.0	41.0	29.0	41.0
34-35	36.223024923732225	40.0	35.0	41.0	29.0	41.0
36-37	36.10002440568698	40.0	35.0	41.0	28.0	41.0
38-39	35.939800149657515	40.0	35.0	41.0	27.5	41.0
40-41	35.74175997237092	40.0	35.0	41.0	27.0	41.0
42-43	35.58030599205664	39.5	35.0	41.0	26.5	41.0
44-45	35.38009946468658	39.0	35.0	41.0	26.0	41.0
46-47	35.124533241236406	39.0	35.0	41.0	24.5	41.0
48-49	34.891037356818046	39.0	34.5	41.0	24.0	41.0
50-51	34.67703816266621	39.0	34.0	41.0	23.5	41.0
52-53	34.475494387843206	38.5	34.0	41.0	23.0	41.0
54-55	34.25680584815518	38.0	34.0	40.0	23.0	41.0
56-57	33.99313095032522	38.0	33.5	40.0	22.0	41.0
58-59	33.850217003396075	37.0	33.5	40.0	22.0	41.0
60-61	33.72449594197893	37.0	33.0	40.0	22.0	41.0
62-63	33.456875151096526	36.5	33.0	40.0	22.0	41.0
64-65	33.157013987221546	36.0	33.0	39.0	21.5	41.0
66-67	32.85442284003914	35.5	33.0	39.0	20.5	41.0
68-69	32.53417579001899	35.0	33.0	39.0	20.0	41.0
70-71	32.23466482472803	35.0	32.5	37.5	20.0	40.0
72-73	31.919183100212976	35.0	32.0	37.0	19.0	39.0
74-75	31.62900201462039	35.0	32.0	36.5	18.0	39.0
76-77	31.34192482587924	35.0	32.0	36.0	18.0	39.0
78-79	31.068139296609683	35.0	32.0	36.0	17.0	37.0
80-81	30.84398664594486	35.0	32.0	35.0	15.0	37.0
82-83	30.6602995452714	35.0	31.5	35.0	13.5	36.5
84-85	30.47257054049387	35.0	31.0	35.0	11.5	36.0
86-87	30.330899211420018	35.0	31.0	35.0	10.5	36.0
88-89	30.2307741898348	35.0	31.0	35.0	9.0	36.0
90-91	30.13413756979221	35.0	31.0	35.0	7.5	35.0
92-93	30.197735796926267	35.0	31.5	35.0	7.5	35.0
94-95	30.169165486674725	35.0	32.0	35.0	7.0	35.0
96-97	30.151229148678986	35.0	32.0	35.0	7.0	35.0
98-99	30.114182236804236	35.0	32.0	35.0	7.0	35.0
100-101	30.067190007482875	35.0	32.0	35.0	3.5	35.0
102-103	29.185346917630806	33.5	29.5	35.0	2.0	35.0
104-105	29.893086513555517	34.5	31.0	35.0	2.0	35.0
106-107	29.999763080642374	35.0	31.5	35.0	2.0	35.0
108-109	30.007919875669142	35.0	32.0	35.0	2.0	35.0
110-111	29.972282967823634	35.0	32.0	35.0	2.0	35.0
112-113	29.922806193518678	35.0	32.0	35.0	2.0	35.0
114-115	29.85769757669948	35.0	32.0	35.0	2.0	35.0
116-117	29.78575697922063	35.0	32.0	35.0	2.0	35.0
118-119	29.695440741380303	35.0	31.5	35.0	2.0	35.0
120-121	29.571155240891038	35.0	31.0	35.0	2.0	35.0
122-123	29.45054671041271	35.0	31.0	35.0	2.0	35.0
124-125	28.53639831923099	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34638.0
3	13264.0
4	7399.0
5	6347.0
6	6483.0
7	7593.0
8	8804.0
9	9554.0
10	11028.0
11	11863.0
12	12269.0
13	13855.0
14	16895.0
15	14180.0
16	8509.0
17	7222.0
18	6544.0
19	6096.0
20	5980.0
21	6194.0
22	7668.0
23	9953.0
24	14086.0
25	19059.0
26	24005.0
27	26934.0
28	32260.0
29	38679.0
30	44924.0
31	54001.0
32	67113.0
33	87241.0
34	121142.0
35	193830.0
36	389662.0
37	628894.0
38	197150.0
39	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.69983024482182	17.67745225232203	12.639983476302048	24.9827340265541
2	16.852344663428795	19.740964113408097	37.48749188958694	25.919199333576167
3	19.159587231916063	24.21426910065655	28.98357816778318	27.642565499644206
4	13.52522652777816	15.536216338750833	35.25581305341844	35.682744080052565
5	14.476143539479805	37.17545403042683	33.18721759837134	15.161184831722021
6	33.78583021832967	34.7566244106978	16.773719010396036	14.683826360576488
7	29.765690052834415	31.37430272572528	20.32913740632392	18.53086981511639
8	28.16534623737518	33.1597372526843	19.115217203096822	19.559699306843697
9	27.95765029209712	14.213612656099043	18.735384782582667	39.09335226922117
10-11	26.566814806559663	24.68973399982086	26.63347430454802	22.109976889071458
12-13	26.893747255684865	22.292093448204483	27.082876187322835	23.731283108787817
14-15	24.386974371018137	23.340632216857426	24.53302111610751	27.739372296016928
16-17	24.306149606097957	26.39264571888706	24.977716148751817	24.32348852626317
18-19	24.360556320893718	25.36879180977902	26.911216193844258	23.35943567548301
20-21	25.40762283719495	24.29235920179426	26.7004241561779	23.599593804832892
22-23	25.27363672095997	23.922463666500647	26.523448770209168	24.280450842330218
24-25	24.74579713555621	24.42525870467933	26.49595538989286	24.332988769871598
26-27	24.31515058294145	24.570740813315744	26.693705503410285	24.42040310033252
28-29	24.469065236579056	24.634677598187842	26.64049383432454	24.255763330908557
30-31	24.75071274278161	24.535554235235054	26.454110418710474	24.25962260327286
32-33	24.47023226307106	24.575627402736313	26.378305687353848	24.57583464683878
34-35	24.625582079247753	24.665283562779813	26.51393570432251	24.19519865364992
36-37	24.812885722068952	24.46722256161939	26.351824811803436	24.368066904508222
38-39	24.789816691230655	24.6573784082535	26.190770081061167	24.362034819454678
40-41	24.751074750107648	24.57181541238858	26.312829994911198	24.36427984259257
42-43	24.6575011265615	24.617228136014486	26.169108336647202	24.556162400776817
44-45	24.653181966083988	24.640125124609934	26.031265954044063	24.67542695526201
46-47	24.809635888554297	24.731295705636185	25.816360202726525	24.642708203082993
48-49	24.90900746110033	25.00077722193935	25.382295321768733	24.707919995191588
50-51	24.829745872658616	25.17030018242418	25.342661329803672	24.657292615113526
52-53	25.00995427596316	24.982342530723535	25.414481312559662	24.593221880753646
54-55	24.80427915755237	25.022930144291227	25.480657965608582	24.692132732547815
56-57	24.729832021114635	25.065384485183394	25.314325679101675	24.890457814600296
58-59	24.796693580755967	24.85156875527768	25.561100386567166	24.79063727739919
60-61	24.905905262304625	24.988140286364878	25.6507183071606	24.455236144169902
62-63	24.755870979390085	25.04366273210795	25.572889127979703	24.627577160522257
64-65	24.729145442587676	24.819000440294094	25.656480336907883	24.795373780210348
66-67	24.72640240140942	25.024644335210855	25.47391845275253	24.775034810627194
68-69	24.74377134950889	24.968052740555553	25.447658885943962	24.840517023991595
70-71	24.673585352306755	24.829251173711082	25.67767595534141	24.819487518640752
72-73	24.879200854615238	25.11291205444557	25.29508658413891	24.712800506800285
74-75	24.678780392546987	25.253862280633882	25.29740850457063	24.769948822248505
76-77	24.689496237479855	24.940975673895117	25.3235486394297	25.045979449195325
78-79	24.690988684209437	24.96880522994311	25.422160009249033	24.91804607659842
80-81	24.707839259835662	25.169520818849172	25.21141022207371	24.911229699241456
82-83	24.82661211587561	25.115453765073003	25.196575347512614	24.861358771538782
84-85	24.5457677138157	25.185943714434668	25.148432592352428	25.119855979397204
86-87	24.41965157449129	25.198331190291096	25.347776148492564	25.03424108672505
88-89	24.50500852294055	25.033242001308952	25.26608569734971	25.195663778400785
90-91	24.463851573686068	24.998008167205267	25.359094063785626	25.17904619532304
92-93	24.552595088942176	25.08787837899448	25.27719739696712	25.082329135096227
94-95	24.46203054098469	24.897909278736968	25.36887936336376	25.27118081691458
96-97	24.438719463688674	24.91844051401414	25.412459945537215	25.23038007675997
98-99	24.30090394595665	24.93315957432112	25.292757841028923	25.473178638693305
100-101	24.32041724473407	25.027706938535843	25.37400625176299	25.277869564967098
102-103	24.401478936120146	24.784406240487833	25.47598248448927	25.338132338902753
104-105	24.248139257194882	24.9667638156383	25.334284407797274	25.45081251936955
106-107	24.319914239119697	24.90947424261018	25.291009774041907	25.479601744228216
108-109	24.302266805297926	24.876964758414307	25.305270866364765	25.515497569923006
110-111	24.265787012499015	25.05172427290155	25.28134642898751	25.40114228561193
112-113	24.207247868175866	25.032253964365097	25.327588140304226	25.432910027154808
114-115	24.031550350632365	25.171298363193745	25.33332350944118	25.46382777673271
116-117	23.931548359168488	25.159086513221517	25.313107310288927	25.596257817321067
118-119	24.005570545739403	24.887067040431127	25.352453531581393	25.754908882248078
120-121	23.781323586669682	24.979455885471484	25.490796536017076	25.748423991841758
122-123	23.81328445416277	25.026898202649782	25.402264261112762	25.757553082074686
124-125	23.735855270354765	25.091146927857416	25.31720927233304	25.855788529454777
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	46.0
2	86.0
3	126.0
4	91.5
5	79.0
6	148.0
7	333.5
8	570.5
9	634.0
10	511.0
11	398.5
12	420.5
13	512.5
14	634.0
15	763.5
16	880.0
17	1020.5
18	1243.0
19	1429.0
20	1557.5
21	1768.5
22	2030.0
23	2407.5
24	2825.0
25	3397.5
26	4541.5
27	5969.0
28	7136.0
29	8937.0
30	11386.0
31	13709.0
32	16509.0
33	19275.5
34	22502.0
35	25957.0
36	30126.0
37	34768.0
38	38049.0
39	41976.5
40	46025.5
41	48820.5
42	52126.0
43	57998.0
44	63894.0
45	68889.0
46	75048.5
47	80586.0
48	86073.5
49	91353.0
50	92621.0
51	92693.5
52	92488.0
53	92512.5
54	93604.0
55	92197.0
56	87444.0
57	80060.5
58	74391.0
59	68879.0
60	61645.0
61	54816.0
62	47589.5
63	37654.5
64	27902.5
65	21673.5
66	16820.0
67	12957.5
68	10207.0
69	8074.0
70	6774.5
71	5629.0
72	4439.0
73	3895.0
74	3165.0
75	2368.5
76	1580.0
77	855.0
78	501.0
79	294.5
80	152.5
81	70.5
82	36.5
83	20.0
84	9.5
85	3.0
86	3.0
87	2.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1206009324814367
2	0.0015656478443561849
3	0.01980084038450469
4	0.05760663097910551
5	0.023714959995395152
6	0.0190180164623266
7	0.014413169861278996
8	0.005019282795141887
9	0.0040062165429114146
10-11	0.006515857940482358
12-13	0.00416738617394808
14-15	0.0027629079606285617
16-17	0.009647153629194728
18-19	0.004996258562136649
20-21	0.011742358832671386
22-23	0.00821965118286997
24-25	0.012824497783917572
26-27	0.008311748114890924
28-29	0.01333103091003281
30-31	0.020031082714557072
32-33	0.012548206987854717
34-35	0.019409428423415642
36-37	0.013423127842053762
38-39	0.019570598054452312
40-41	0.008749208541990445
42-43	0.008956426639037587
44-45	0.01609393887066137
46-47	0.014988775686409947
48-49	0.01982386461750993
50-51	0.014367121395268521
52-53	0.02060668853968802
54-55	0.016416278132734705
56-57	0.019777816151499455
58-59	0.015357163414493754
60-61	0.018833822598284693
62-63	0.020123179646578022
64-65	0.01607091463765613
66-67	0.010752316813446152
68-69	0.021573706325908017
70-71	0.01413687906521614
72-73	0.016370229666724228
74-75	0.016991883957865654
76-77	0.012640303919875669
78-79	0.0270764980141599
80-81	0.019869913083520405
82-83	0.008887353940021873
84-85	0.01238703735681805
86-87	0.01183445576469234
88-89	0.019616646520462785
90-91	0.011880504230702815
92-93	0.007275657629655212
94-95	0.006884245668566166
96-97	0.002325447533529039
98-99	0.001611696310366661
100-101	0.010936510677488055
102-103	0.0029471018246704657
104-105	0.002118229436481897
106-107	2.5326656305761815E-4
108-109	0.01830426523916422
110-111	6.446785241466644E-4
112-113	0.008588038910953779
114-115	0.002233350601508087
116-117	0.007597996891728544
118-119	0.00939388706613711
120-121	0.003614804581822368
122-123	5.295573591204743E-4
124-125	0.024981292810683245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2171625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.53952313895305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6644157764683	41.581157855374954
2	11.325084819710247	12.126792815109653
3	3.945416560536383	6.337071637069386
4	1.9759044081502433	4.2315591912207715
5	1.1518849463444396	3.083568536910991
6	0.7673156394704626	2.464902806058563
7	0.5303899237745822	1.9877776517617747
8	0.398340810247694	1.7061581621956576
9	0.3067154813806388	1.477926055120845
>10	1.7909926115703225	17.390955462708263
>50	0.10521357750400645	3.823613393188753
>100	0.037635511173950364	3.3308456971765454
>500	4.3120854295236187E-4	0.1505714558550168
>1k	2.5872512577140687E-4	0.3070992802489064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2804	0.12911989869337478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2499	0.11507511656017959	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	4.604846601047603E-5
10-11	0.0	0.0	0.0	0.0	4.604846601047603E-5
12-13	0.0	0.0	0.0	0.0	1.1512116502619007E-4
14-15	0.0	0.0	0.0	0.0	1.6116963103666608E-4
16-17	0.0	0.0	0.0	0.0	2.9931502906809415E-4
18-19	0.0	0.0	0.0	0.0	3.2233926207333216E-4
20-21	0.0	0.0	0.0	2.3024233005238014E-5	3.683877280838082E-4
22-23	0.0	0.0	0.0	1.3814539803142807E-4	3.683877280838082E-4
24-25	0.0	0.0	0.0	2.7629079606285615E-4	3.683877280838082E-4
26-27	0.0	0.0	0.0	3.683877280838082E-4	3.683877280838082E-4
28-29	0.0	0.0	0.0	0.0012202843492776147	3.683877280838082E-4
30-31	0.0	0.0	0.0	0.004190410406953319	3.683877280838082E-4
32-33	0.0	0.0	0.0	0.011166753007540435	3.683877280838082E-4
34-35	0.0	0.0	0.0	0.020675761238703735	4.144361940942842E-4
36-37	0.0	0.0	0.0	0.036171070051228923	4.144361940942842E-4
38-39	0.0	0.0	0.0	0.057882921775168364	4.604846601047603E-4
40-41	0.0	0.0	0.0	0.08396937777010303	5.525815921257123E-4
42-43	0.0	0.0	0.0	0.10752316813446153	5.756058251309503E-4
44-45	0.0	0.0	0.0	0.13614228975997236	5.986300581361883E-4
46-47	0.0	0.0	0.0	0.17086283313187128	5.986300581361883E-4
48-49	0.0	0.0	0.0	0.20330397743625167	6.446785241466643E-4
50-51	0.0	0.0	0.0	0.23993553214758534	6.907269901571404E-4
52-53	0.0	0.0	0.0	0.27622172336384043	7.828239221780924E-4
54-55	0.0	4.604846601047603E-5	0.0	0.31607667069590745	8.979450872042825E-4
56-57	0.0	4.604846601047603E-5	0.0	0.3607667069590744	9.209693202095206E-4
58-59	0.0	4.604846601047603E-5	0.0	0.4131468370459909	9.439935532147586E-4
60-61	0.0	4.604846601047603E-5	0.0	0.47307891555862547	9.670177862199966E-4
62-63	0.0	4.604846601047603E-5	0.0	0.5359120474299199	0.0010591147182409486
64-65	0.0	4.604846601047603E-5	0.0	0.5989984458642721	0.0011051631842514246
66-67	0.0	4.604846601047603E-5	0.0	0.67134058596673	0.0011512116502619006
68-69	0.0	4.604846601047603E-5	0.0	0.7564841996201002	0.0011512116502619006
70-71	0.0	4.604846601047603E-5	0.0	0.8486502043400679	0.0011512116502619006
72-73	0.0	4.604846601047603E-5	0.0	0.9459045645541933	0.0012202843492776145
74-75	0.0	4.604846601047603E-5	0.0	1.0446785241466645	0.0012433085822828526
76-77	0.0	4.604846601047603E-5	0.0	1.1571979508432624	0.0012433085822828526
78-79	0.0	4.604846601047603E-5	0.0	1.2854659529154435	0.0012433085822828526
80-81	0.0	4.604846601047603E-5	0.0	1.4222068727335522	0.0013123812812985667
82-83	0.0	4.604846601047603E-5	0.0	1.5680423645887296	0.0013354055143038049
84-85	0.0	4.604846601047603E-5	0.0	1.7200253266563057	0.0013354055143038049
86-87	0.0	4.604846601047603E-5	0.0	1.8867668220802396	0.0013354055143038049
88-89	0.0	4.604846601047603E-5	0.0	2.0726875035975363	0.0013354055143038049
90-91	0.0	4.604846601047603E-5	0.0	2.2781787831692855	0.0013354055143038049
92-93	0.0	4.604846601047603E-5	0.0	2.5028722730674033	0.0013814539803142809
94-95	0.0	4.604846601047603E-5	0.0	2.7325620215276576	0.0015195993783457089
96-97	0.0	4.604846601047603E-5	0.0	2.9721061417141543	0.0015656478443561849
98-99	0.0	4.604846601047603E-5	0.0	3.236723651643355	0.001611696310366661
100-101	0.0	4.604846601047603E-5	0.0	3.543222241409083	0.001611696310366661
102-103	0.0	1.3814539803142807E-4	0.0	3.871524779830772	0.001611696310366661
104-105	0.0	1.3814539803142807E-4	0.0	4.210326368502849	0.001611696310366661
106-107	0.0	1.3814539803142807E-4	0.0	4.5592355954642265	0.001657744776377137
108-109	0.0	1.3814539803142807E-4	0.0	4.928244977839176	0.0017268174753928508
110-111	0.0	1.3814539803142807E-4	0.0	5.337546767973292	0.001864962873424279
112-113	0.0	1.3814539803142807E-4	0.0	5.778092442295516	0.0018879871064295172
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2855	0.0	76.85137	1
GTATCAA	5715	0.0	58.42271	1
TCAACGC	7785	0.0	42.44175	4
CAACGCA	8115	0.0	40.62749	5
ATCAACG	8320	0.0	39.85573	3
AACGCAG	8605	0.0	38.30519	6
TATCAAC	8730	0.0	37.917477	2
GTACATG	11305	0.0	35.177517	1
ACGCAGA	9790	0.0	33.66478	7
TACATGG	11875	0.0	33.089424	2
ACATGGG	11915	0.0	32.27731	3
CGCAGAG	10480	0.0	31.503616	8
CATGGGG	9840	0.0	29.948078	4
GCAGAGT	11410	0.0	28.570879	9
AGAGTAC	10495	0.0	27.208994	10-11
ATGGGGG	5495	0.0	25.342352	5
CAGAGTA	11050	0.0	24.496433	10-11
GAGTACT	5595	0.0	22.807932	12-13
GTATAGA	645	0.0	22.185102	1
ATGGGGA	3935	0.0	22.080397	5
>>END_MODULE