FastQCFastQC Report
Mon 15 Aug 2016
SRR2921490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833597
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.070.6086041
GTATCAA42050.059.58761
TCAACGC55500.043.4205364
CAACGCA56650.042.6441275
AACGCAG60150.040.352896
TATCAAC60700.039.990532
ATCAACG60300.039.8611453
GTACATG98600.035.7342451
ACGCAGA71450.033.8867957
TACATGG103500.033.4555632
ACATGGG105050.032.7920233
CGCAGAG75650.031.7695268
CATGGGG89100.030.051624
GCAGAGT83900.028.2193859
CGTATAC1306.383432E-527.459563
AGAGTAC75300.025.8332610-11
ATGGGGG47500.025.6799015
CAGAGTA79600.024.47511310-11
ATGGGGA34650.023.8695765
GTGGTAT9750.021.3650171
Produced by FastQC (version 0.11.5)