FastQCFastQC Report
Mon 15 Aug 2016
SRR2921490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833597
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32080.17495665623362167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.1503056560411039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.075.9723361
GTATCAA52050.059.78431
TCAACGC70850.043.1079524
CAACGCA72500.042.1096345
TATCAAC75350.040.928552
AACGCAG76450.040.1543056
ATCAACG76150.040.0294763
GTACATG93100.033.4880141
ACGCAGA91400.033.4553077
CGCAGAG95550.031.939998
TACATGG97650.031.7037732
ACATGGG98500.031.007093
CATGGGG83150.029.4279124
GCAGAGT104300.028.5760049
TACCGTA1954.794856E-827.457187
AGAGTAC95350.026.14286810-11
GTGGTAT11950.025.4413241
CAGAGTA101400.024.55372810-11
GTACTAG2752.073648E-923.8450051
ATGGGGG44000.023.8046675
Produced by FastQC (version 0.11.5)