FastQCFastQC Report
Mon 15 Aug 2016
SRR2921489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824283
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.1075491028530113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41300.061.3752861
GGTATCA20500.060.6633531
TCAACGC53300.046.3241274
CAACGCA54700.045.2472655
ATCAACG57050.043.5872343
AACGCAG56650.043.4797176
TATCAAC59300.042.5341642
GTACATG95250.038.5437661
ACGCAGA66200.037.026587
TACATGG99250.036.681552
ACATGGG101700.035.33093
CGCAGAG70700.034.667028
GCAGAGT75150.031.9008929
CATGGGG81400.031.5751674
ATGGGGG47900.028.8163385
AGAGTAC70350.027.65021510-11
CATGGGT19350.024.905224
CAGAGTA74500.024.31340810-11
GAGTACT30150.022.00108312-13
GTATAGG6000.021.8175221
Produced by FastQC (version 0.11.5)