FastQCFastQC Report
Mon 15 Aug 2016
SRR2921489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824283
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.18253746814501917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.17919368869851882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.077.671611
GTATCAA57150.059.8789981
TCAACGC75300.045.152674
CAACGCA76700.044.2229585
AACGCAG80300.042.3792726
ATCAACG81450.041.9626663
TATCAAC81350.041.7946662
ACGCAGA93500.036.394327
GTACATG96650.035.2836151
CGCAGAG98900.034.3460778
TACATGG101000.033.1327322
ACATGGG100550.032.392733
GTGGTAT11700.032.102111
GCAGAGT107350.031.1992159
CATGGGG83200.030.8458164
AGAGTAC99050.027.53773110-11
ATGGGGG45600.027.143565
CAGAGTA103700.026.64711610-11
GAGTACT55850.026.52316912-13
TGGTATC13000.026.1080132
Produced by FastQC (version 0.11.5)