FastQCFastQC Report
Mon 15 Aug 2016
SRR2921488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577139
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.10157633537690718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.074.119281
GTATCAA39450.057.491761
TCAACGC47550.046.547844
CAACGCA48450.045.4361275
AACGCAG50500.043.586166
ATCAACG52300.042.2051623
TATCAAC55200.040.6345632
GTACATG83000.037.2951971
ACGCAGA61000.035.7899177
TACATGG86950.035.034322
CGCAGAG63150.034.569228
ACATGGG90000.033.582623
GCAGAGT69300.030.9005479
CATGGGG70650.030.8230824
ATGGGGG36950.028.5004795
CAGAGTA67650.026.731510-11
AGAGTAC63350.024.93075810-11
CATGGGT16050.024.8374424
GAGTACT26350.021.44738612-13
GAGTACA60050.020.65496312-13
Produced by FastQC (version 0.11.5)