##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921488_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1577139 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.8687097332575 31.0 30.0 34.0 25.0 34.0 2 29.895912154857626 31.0 30.0 34.0 25.0 34.0 3 30.012131460828755 31.0 30.0 34.0 25.0 34.0 4 33.39723448599014 37.0 35.0 37.0 27.0 37.0 5 33.43354644073858 37.0 35.0 37.0 27.0 37.0 6 33.495789527746126 37.0 35.0 37.0 27.0 37.0 7 33.30638833989902 37.0 35.0 37.0 26.0 37.0 8 33.26055661549172 37.0 35.0 37.0 26.0 37.0 9 34.717642515973544 39.0 35.0 39.0 27.0 39.0 10-11 34.60531189704902 39.0 35.0 39.0 26.0 39.0 12-13 34.459840889103624 39.0 35.0 39.0 25.0 39.0 14-15 35.604006051464076 40.0 36.0 41.0 25.0 41.0 16-17 35.53403124264887 40.0 36.0 41.0 25.0 41.0 18-19 35.55558419391062 40.0 36.0 41.0 25.0 41.0 20-21 35.60124947769347 40.0 36.0 41.0 25.0 41.0 22-23 35.5674344493415 40.0 36.0 41.0 25.0 41.0 24-25 35.53468083662886 40.0 35.5 41.0 25.0 41.0 26-27 35.4706626999903 40.0 35.0 41.0 25.0 41.0 28-29 35.438011487890414 40.0 35.0 41.0 25.0 41.0 30-31 35.34708830356741 40.0 35.0 41.0 25.0 41.0 32-33 35.24560200464258 40.0 35.0 41.0 25.0 41.0 34-35 35.08086383001118 40.0 35.0 41.0 24.0 41.0 36-37 34.9347391066989 39.5 35.0 41.0 24.0 41.0 38-39 34.806311935726654 39.0 34.5 41.0 23.5 41.0 40-41 34.6512174893906 39.0 34.0 41.0 22.5 41.0 42-43 34.443437452247395 39.0 34.0 41.0 21.5 41.0 44-45 34.23656475427974 39.0 33.5 41.0 19.5 41.0 46-47 33.97950846437759 39.0 33.0 41.0 18.0 41.0 48-49 33.81814095016355 38.0 33.0 41.0 15.5 41.0 50-51 33.618703234147404 38.0 33.0 40.0 14.5 41.0 52-53 33.435657858945845 38.0 33.0 40.0 13.5 41.0 54-55 33.23621887481065 38.0 33.0 40.0 12.0 41.0 56-57 32.99618391276863 37.0 32.5 40.0 8.5 41.0 58-59 32.75791797679216 37.0 32.0 40.0 8.0 41.0 60-61 32.453410574464264 36.0 32.0 40.0 7.0 41.0 62-63 32.1408623463119 36.0 31.5 39.0 6.5 41.0 64-65 31.837939458728748 35.0 31.0 39.0 2.0 41.0 66-67 31.804982629939403 35.0 31.0 39.0 2.0 41.0 68-69 31.55860612159106 35.0 31.0 38.5 2.0 40.5 70-71 31.251541557212143 35.0 31.0 37.5 2.0 40.0 72-73 30.922503026049068 35.0 31.0 37.0 2.0 39.0 74-75 30.62346977660181 35.0 31.0 36.5 2.0 39.0 76-77 30.342162295143297 35.0 31.0 36.0 2.0 39.0 78-79 30.066721132379584 35.0 30.5 35.5 2.0 37.0 80-81 29.826693462022053 35.0 30.0 35.0 2.0 37.0 82-83 29.62987346074125 35.0 30.0 35.0 2.0 36.5 84-85 29.43144865481102 35.0 30.0 35.0 2.0 36.0 86-87 29.27966843759491 35.0 30.0 35.0 2.0 36.0 88-89 29.153211289556594 34.0 30.0 35.0 2.0 36.0 90-91 29.04157052739169 34.0 30.0 35.0 2.0 35.0 92-93 28.942395058393714 34.0 30.0 35.0 2.0 35.0 94-95 28.873512417104642 34.0 30.0 35.0 2.0 35.0 96-97 28.769730505681487 34.0 29.0 35.0 2.0 35.0 98-99 28.710577824782725 34.0 29.0 35.0 2.0 35.0 100-101 28.648937728380314 34.0 29.0 35.0 2.0 35.0 102-103 27.663039846202523 32.5 27.5 34.5 2.0 35.0 104-105 28.483756346143238 34.0 29.0 35.0 2.0 35.0 106-107 28.806833766713016 34.5 30.0 35.0 2.0 35.0 108-109 28.84392973606004 35.0 30.0 35.0 2.0 35.0 110-111 28.782491270585535 35.0 30.0 35.0 2.0 35.0 112-113 28.70885413397297 35.0 30.0 35.0 2.0 35.0 114-115 28.58827788799846 35.0 30.0 35.0 2.0 35.0 116-117 28.526351513722 35.0 30.0 35.0 2.0 35.0 118-119 28.36941290526707 35.0 30.0 35.0 2.0 35.0 120-121 28.198068147449277 34.5 29.5 35.0 2.0 35.0 122-123 28.06990252602973 34.0 29.0 35.0 2.0 35.0 124-125 27.224796926586684 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59428.0 3 10336.0 4 7087.0 5 5856.0 6 6297.0 7 7020.0 8 7931.0 9 8416.0 10 8751.0 11 9444.0 12 9391.0 13 10254.0 14 11684.0 15 10277.0 16 7004.0 17 6167.0 18 5684.0 19 5298.0 20 5047.0 21 5225.0 22 6263.0 23 8369.0 24 12440.0 25 17094.0 26 19889.0 27 21349.0 28 25237.0 29 29796.0 30 33472.0 31 39883.0 32 50458.0 33 65637.0 34 92998.0 35 144690.0 36 275016.0 37 409611.0 38 118170.0 39 170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.901058075876236 17.1899747612019 12.158897231343808 24.750069931578057 2 16.884299664433573 19.431078233085163 38.041347415580404 25.643274686900856 3 19.288163735405796 24.21916372969781 28.804069665302883 27.68860286959351 4 13.28974307583411 15.129020227672306 35.57462268147949 36.006614015014094 5 14.333981333781542 37.31448740838933 33.29424188704496 15.057289370784165 6 33.525351541759676 34.66591559445399 17.239454381561178 14.569278482225153 7 29.857798560859017 31.309372177284306 20.453397915376886 18.379431346479787 8 28.124213091493555 33.27237864721191 19.355237694733095 19.24817056656144 9 27.847538467393097 14.628074537313054 18.888599951796834 38.63578704349701 10-11 26.44183838318488 24.952951989146133 26.802492228436094 21.802717399232897 12-13 26.57282001092238 22.68389341732955 26.938770591261207 23.804515980486865 14-15 24.115644780670138 23.86637206394955 24.330190573114763 27.687792582265548 16-17 24.2093929336545 26.807395226625097 24.818219443312405 24.164992396407996 18-19 24.298584004663947 25.856933784249282 26.40884272308064 23.435639488006128 20-21 25.423845488159863 24.88805098961783 26.086752393786433 23.601351128435873 22-23 25.19504182197848 24.524247041026427 25.94386919200426 24.33684194499083 24-25 24.553443684701072 25.015052816510448 26.050572387709796 24.38093111107868 26-27 24.228382530938287 25.039965388704637 26.18573502059169 24.545917059765383 28-29 24.317489337703773 25.068274855377837 26.204634887325078 24.409600919593313 30-31 24.64856525430906 25.031441114796465 25.98747303518011 24.332520595714367 32-33 24.279340121264106 25.09882764496783 25.974937651825975 24.646894581942092 34-35 24.558978096541157 25.14016729368497 26.09119262877662 24.209661980997254 36-37 24.670121382389578 24.949224433554665 25.93406265750893 24.446591526546825 38-39 24.646356148669998 25.160167546229385 25.84816789137157 24.345308413729043 40-41 24.648004369535307 25.058028925968472 26.04006422354157 24.25390248095465 42-43 24.563970179119423 25.06723401879571 25.887111560571523 24.481684241513353 44-45 24.482070695775835 25.12231122294093 25.88331653665498 24.51230154462825 46-47 24.527144275639785 25.20634598363698 25.735558636267026 24.53095110445621 48-49 24.71920305082305 25.35554186016342 25.44076977266953 24.484485316344 50-51 24.651842171623056 25.530781101068463 25.32124409031724 24.496132636991245 52-53 24.736735584695072 25.343331819116187 25.50635336752588 24.41357922866286 54-55 24.670840101129187 25.347508674038526 25.62665745580356 24.354993769028727 56-57 24.52539946245665 25.452493639112657 25.421759658367538 24.600347240063154 58-59 24.70803094502472 25.26492974378151 25.433255403025296 24.593783908168472 60-61 24.625870231486648 25.370514375310798 25.68035991556471 24.323255477637844 62-63 24.47532840640469 25.462046251725013 25.615271385467942 24.44735395640236 64-65 24.536278921215544 25.14067123195997 25.71325175178937 24.60979809503511 66-67 24.44592726450462 25.42574079072014 25.557450309230354 24.57088163554489 68-69 24.532532894423884 25.43518315906979 25.50063901917692 24.531644927329406 70-71 24.46344403209947 25.162305325593938 25.728613569321535 24.64563707298506 72-73 24.652921919046776 25.499507968437673 25.42615514834997 24.421414964165578 74-75 24.4208734218325 25.645443865952178 25.461398327446922 24.4722843847684 76-77 24.50333940112772 25.28966847009127 25.51248549120593 24.69450663757508 78-79 24.447408597015002 25.414510943367986 25.55008603865111 24.587994420965902 80-81 24.49606562388417 25.611275902287183 25.356859044245006 24.53579942958364 82-83 24.631488760989857 25.554435531817678 25.37648599122999 24.437589715962474 84-85 24.495623867599246 25.575784364894798 25.2694772930065 24.65911447449946 86-87 24.243920319675098 25.65210663531304 25.43509183810011 24.66888120691175 88-89 24.421542637527114 25.45374116871092 25.4271363157828 24.697579877979173 90-91 24.38215941720188 25.465488945707648 25.449381842348238 24.702969794742234 92-93 24.475886581269567 25.651980762663307 25.341325742573943 24.530806913493183 94-95 24.409068324438746 25.37625353106785 25.476989439059217 24.73768870543419 96-97 24.419294084821427 25.4246778612013 25.398868709415584 24.75715934456169 98-99 24.31914245320199 25.42798232172146 25.34481616093207 24.908059064144478 100-101 24.307640699242786 25.53287553354137 25.424978265184407 24.734505502031435 102-103 24.406704213849 25.36054933362417 25.561020089560305 24.671726362966524 104-105 24.211769159530093 25.529785923336195 25.429090216188232 24.82935470094548 106-107 24.301496104459396 25.551658200673373 25.428132166515375 24.718713528351856 108-109 24.34249180126189 25.517472515242535 25.32656232610479 24.813473357390787 110-111 24.30929697479668 25.659235736440767 25.37303400781657 24.658433280945978 112-113 24.291888711056096 25.62840358855862 25.438424057648263 24.641283642737026 114-115 24.071176534558944 25.837115018720567 25.473578614192633 24.618129832527853 116-117 24.139397134911775 25.96470747508195 25.266237988813707 24.629657401192567 118-119 24.29025149697554 25.541984276751545 25.32419904419836 24.843565182074556 120-121 23.93035261177706 25.694668268121763 25.540292239041033 24.834686881060144 122-123 24.060830868532655 25.783733679118942 25.39263188705267 24.762803565295734 124-125 24.02139259191624 25.858544355165975 25.364944758948138 24.755118293969645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 111.0 1 129.5 2 155.0 3 176.5 4 171.0 5 145.5 6 158.0 7 209.0 8 291.5 9 366.0 10 389.0 11 431.5 12 521.5 13 605.5 14 653.0 15 723.5 16 819.5 17 894.5 18 1015.5 19 1122.0 20 1203.5 21 1407.5 22 1661.5 23 2002.0 24 2481.0 25 3134.0 26 4230.5 27 5473.0 28 6353.0 29 7669.5 30 9627.5 31 11475.5 32 13741.0 33 16315.0 34 18950.0 35 21513.0 36 24714.0 37 27964.5 38 29699.5 39 31705.5 40 34224.5 41 36215.0 42 38012.5 43 41784.5 44 45937.5 45 48989.5 46 52597.0 47 56159.0 48 60295.5 49 64651.0 50 65163.5 51 64444.0 52 64355.5 53 64283.0 54 64769.5 55 63908.5 56 60927.0 57 56241.5 58 52939.5 59 49593.0 60 44556.5 61 39607.0 62 34269.0 63 26900.0 64 20192.5 65 15995.5 66 12425.0 67 9342.0 68 7360.5 69 6014.0 70 5132.0 71 4334.0 72 3536.5 73 3172.0 74 2612.5 75 2012.5 76 1456.5 77 870.5 78 516.5 79 331.5 80 212.5 81 111.0 82 67.0 83 43.5 84 26.0 85 25.0 86 20.0 87 10.5 88 10.0 89 10.0 90 9.0 91 9.0 92 10.0 93 11.0 94 11.5 95 7.5 96 5.0 97 5.0 98 4.5 99 8.0 100 23.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037916759397871716 2 0.025489192772482322 3 0.025362380868141618 4 0.03087869870696242 5 0.03132254037215489 6 0.046032721275677035 7 0.04077002724553765 8 0.03538052131105755 9 0.03037145108959959 10-11 0.03538052131105755 12-13 0.035507333215398264 14-15 0.035031788574120606 16-17 0.03687056118706088 18-19 0.0502175141189204 20-21 0.06182080336609519 22-23 0.05544850517297461 24-25 0.06480088311810182 26-27 0.04913961293202438 28-29 0.05009070221457969 30-31 0.049424939716790975 32-33 0.05769941647502218 34-35 0.03994574986732304 36-37 0.05322929684701222 38-39 0.0619476152704359 40-41 0.04882258317117261 42-43 0.05884072361408855 44-45 0.059252862303195854 46-47 0.06492769502244254 48-49 0.04929812781245027 50-51 0.05113690042539053 52-53 0.06131355574873236 54-55 0.04688870162997681 56-57 0.04489141413661066 58-59 0.04631804806044363 60-61 0.03468305583718366 62-63 0.04441586949533301 64-65 0.043084344499755574 66-67 0.0357292540479945 68-69 0.031956599893858434 70-71 0.050027296262409336 72-73 0.03249555048730644 74-75 0.03975553201081198 76-77 0.0322736296547102 78-79 0.03309790703292481 80-81 0.0251087570594602 82-83 0.01822921124897679 84-85 0.018387726129402673 86-87 0.018260914225061962 88-89 0.0227627368291571 90-91 0.012364160673219039 92-93 0.019624142196724575 94-95 0.01543934935348121 96-97 0.011349665438493374 98-99 0.011127744605897133 100-101 0.01115944758198231 102-103 0.006404001169205757 104-105 0.007006357714824121 106-107 0.009098754136445805 108-109 0.013917606501392713 110-111 0.006625922001801997 112-113 0.008052555925634964 114-115 0.008274476758231203 116-117 0.007577011284357308 118-119 0.24094261824734536 120-121 0.35833873869075583 122-123 0.0278669159788706 124-125 0.003233703560688056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1577139.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.04366728890867 #Duplication Level Percentage of deduplicated Percentage of total 1 81.79589149510444 49.11325294531726 2 9.572422682131968 11.495267253494694 3 3.115186127301619 5.611415981121669 4 1.564014226203145 3.756365993330463 5 0.9310316759089727 2.7951278091856704 6 0.5966607118450655 2.149541835983311 7 0.41653127883855506 1.7507045865404092 8 0.309410036242747 1.4862490617606938 9 0.23951757209161534 1.2943362067664534 >10 1.3601819973387366 14.593025615903155 >50 0.07160835488380497 2.8683382660776213 >100 0.026799472753105825 2.664648346410282 >500 4.253539178075388E-4 0.16262473457713758 >1k 3.190154383556541E-4 0.25910136353122276 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1602 0.10157633537690718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 6.340595217035404E-5 0.0 0.0 0.0 9.510892825553106E-5 14-15 6.340595217035404E-5 0.0 0.0 0.0 1.585148804258851E-4 16-17 6.340595217035404E-5 0.0 0.0 0.0 1.9021785651106212E-4 18-19 6.340595217035404E-5 0.0 0.0 0.0 1.9021785651106212E-4 20-21 6.340595217035404E-5 0.0 0.0 3.170297608517702E-5 2.2192083259623915E-4 22-23 1.268119043407081E-4 0.0 0.0 6.340595217035404E-5 2.853267847665932E-4 24-25 1.268119043407081E-4 0.0 0.0 1.585148804258851E-4 3.170297608517702E-4 26-27 1.268119043407081E-4 0.0 0.0 4.7554464127765527E-4 3.8043571302212424E-4 28-29 1.268119043407081E-4 0.0 0.0 0.0014583368999181428 4.1213868910730127E-4 30-31 1.268119043407081E-4 0.0 0.0 0.003804357130221242 5.072476173628322E-4 32-33 1.268119043407081E-4 0.0 0.0 0.009162160088616159 5.706535695331863E-4 34-35 1.268119043407081E-4 0.0 0.0 0.016200220779525456 6.974654738738944E-4 36-37 1.268119043407081E-4 0.0 0.0 0.02767669812235954 6.974654738738944E-4 38-39 1.268119043407081E-4 0.0 0.0 0.046444859964784334 7.291684499590714E-4 40-41 1.268119043407081E-4 0.0 0.0 0.06714690334840492 7.608714260442485E-4 42-43 1.268119043407081E-4 0.0 0.0 0.08566144138214832 8.242773782146025E-4 44-45 1.268119043407081E-4 0.0 0.0 0.10896312880475342 9.193863064701336E-4 46-47 1.268119043407081E-4 0.0 0.0 0.1362593912140908 9.827922586404876E-4 48-49 1.268119043407081E-4 0.0 0.0 0.16479206969075016 0.0010144952347256647 50-51 1.268119043407081E-4 0.0 0.0 0.20007748207355217 0.0011413071390663726 52-53 1.268119043407081E-4 0.0 0.0 0.23567992421720596 0.0011730101151515497 54-55 1.268119043407081E-4 0.0 0.0 0.2733747627824814 0.0012364160673219039 56-57 1.268119043407081E-4 0.0 0.0 0.3152543941910003 0.0012998220194922578 58-59 1.268119043407081E-4 0.0 0.0 0.35932153094939634 0.0013315249955774349 60-61 1.268119043407081E-4 0.0 0.0 0.40814411412056895 0.001426633923832966 62-63 1.268119043407081E-4 3.170297608517702E-5 0.0 0.46156362882409224 0.001521742852088497 64-65 1.268119043407081E-4 6.340595217035404E-5 0.0 0.5148563316232748 0.001521742852088497 66-67 1.268119043407081E-4 6.340595217035404E-5 0.0 0.5780403629610327 0.001521742852088497 68-69 1.268119043407081E-4 6.340595217035404E-5 0.0 0.6488965145114032 0.001521742852088497 70-71 1.268119043407081E-4 6.340595217035404E-5 0.0 0.7301195392416268 0.0015534458281736738 72-73 1.268119043407081E-4 6.340595217035404E-5 0.0 0.8114693758761911 0.001743663684684736 74-75 1.268119043407081E-4 6.340595217035404E-5 0.0 0.8960529160714432 0.0017753666607699132 76-77 1.268119043407081E-4 6.340595217035404E-5 0.0 0.9910350324226337 0.0017753666607699132 78-79 1.268119043407081E-4 6.340595217035404E-5 0.0 1.097303408260147 0.0017753666607699132 80-81 1.268119043407081E-4 6.340595217035404E-5 0.0 1.2141605781101097 0.0017753666607699132 82-83 1.585148804258851E-4 6.340595217035404E-5 0.0 1.3425259282789912 0.0019655845172809753 84-85 1.9021785651106212E-4 6.340595217035404E-5 0.0 1.4692427236914438 0.0020289904694513294 86-87 1.9021785651106212E-4 6.340595217035404E-5 0.0 1.607340887518475 0.0020289904694513294 88-89 1.9021785651106212E-4 6.340595217035404E-5 0.0 1.764397431044442 0.0020289904694513294 90-91 1.9021785651106212E-4 6.340595217035404E-5 0.0 1.9413317405758148 0.0020289904694513294 92-93 1.9021785651106212E-4 1.268119043407081E-4 0.0 2.1315178941107917 0.0022826142781327452 94-95 1.9021785651106212E-4 1.268119043407081E-4 0.0 2.333909693438562 0.0022826142781327452 96-97 1.9021785651106212E-4 1.268119043407081E-4 0.0 2.5350016707468397 0.0023777232063882763 98-99 1.9021785651106212E-4 1.268119043407081E-4 0.0 2.763802049153562 0.0024094261824734536 100-101 1.9021785651106212E-4 1.268119043407081E-4 0.0 3.0207229673478366 0.0024094261824734536 102-103 1.9021785651106212E-4 1.268119043407081E-4 0.0 3.2991702063039465 0.002441129158558631 104-105 1.9021785651106212E-4 1.268119043407081E-4 0.0 3.5880160214159944 0.0024728321346438077 106-107 1.9021785651106212E-4 1.268119043407081E-4 0.0 3.8865946501861917 0.0025362380868141614 108-109 1.9021785651106212E-4 1.268119043407081E-4 0.0 4.196808271179648 0.0026630499911548698 110-111 1.9021785651106212E-4 1.268119043407081E-4 0.0 4.543733938479741 0.0027581589194104004 112-113 1.9021785651106212E-4 1.268119043407081E-4 0.0 4.924042839597524 0.0027898618954955777 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2040 0.0 74.11928 1 GTATCAA 3945 0.0 57.49176 1 TCAACGC 4755 0.0 46.54784 4 CAACGCA 4845 0.0 45.436127 5 AACGCAG 5050 0.0 43.58616 6 ATCAACG 5230 0.0 42.205162 3 TATCAAC 5520 0.0 40.634563 2 GTACATG 8300 0.0 37.295197 1 ACGCAGA 6100 0.0 35.789917 7 TACATGG 8695 0.0 35.03432 2 CGCAGAG 6315 0.0 34.56922 8 ACATGGG 9000 0.0 33.58262 3 GCAGAGT 6930 0.0 30.900547 9 CATGGGG 7065 0.0 30.823082 4 ATGGGGG 3695 0.0 28.500479 5 CAGAGTA 6765 0.0 26.7315 10-11 AGAGTAC 6335 0.0 24.930758 10-11 CATGGGT 1605 0.0 24.837442 4 GAGTACT 2635 0.0 21.447386 12-13 GAGTACA 6005 0.0 20.654963 12-13 >>END_MODULE