FastQCFastQC Report
Mon 15 Aug 2016
SRR2921488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577139
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.20150411599738513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30310.1921834410283431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.10087886990303328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.077.8472061
GTATCAA51950.057.5135731
TCAACGC68150.042.984714
ATCAACG69750.042.254773
CAACGCA69500.042.12975
AACGCAG72350.040.868456
TATCAAC73900.039.9637072
GTACATG80450.034.840951
ACGCAGA85000.034.5729987
TACATGG83900.032.9294552
CGCAGAG89150.032.896878
ACATGGG86650.031.4710793
GCAGAGT94600.030.6243489
CATGGGG66850.029.6590714
GTGGTAT10900.028.9979671
CAGAGTA92450.026.63927810-11
AGAGTAC89250.026.46130810-11
ATGGGGG33800.025.7065735
GAGTACT54000.025.39173912-13
CGTATAC1200.001282937924.808673
Produced by FastQC (version 0.11.5)