FastQCFastQC Report
Mon 15 Aug 2016
SRR2921487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743750
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.067.7480551
GTATCAA36300.062.3090131
TCAACGC48050.045.8202634
CAACGCA48600.045.1792835
AACGCAG51700.042.9232646
ATCAACG52150.042.2154773
TATCAAC53550.041.1118052
GTACATG89200.037.70141
ACGCAGA61650.035.8016327
TACATGG93300.035.585812
CGCAGAG65000.034.1346248
ACATGGG95100.033.9111943
CATGGGG82150.031.6535994
GCAGAGT71050.030.22519
TACCGTA2353.4197E-1027.8475387
ATGGGGG44250.027.8359665
AGAGTAC63500.027.30875610-11
CAGAGTA68200.024.81618310-11
TATATAG4350.024.6225195
GTATAGG5500.022.7263931
Produced by FastQC (version 0.11.5)