##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921487_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1743750
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.734216487455196	31.0	30.0	34.0	25.0	34.0
2	29.841216917562726	31.0	30.0	34.0	25.0	34.0
3	29.904805734767024	31.0	30.0	34.0	25.0	34.0
4	33.2898058781362	37.0	35.0	37.0	26.0	37.0
5	33.31090064516129	37.0	35.0	37.0	27.0	37.0
6	33.24983053763441	37.0	35.0	37.0	26.0	37.0
7	33.15038853046595	37.0	35.0	37.0	26.0	37.0
8	33.09766193548387	37.0	35.0	37.0	26.0	37.0
9	34.53491670250896	39.0	35.0	39.0	25.0	39.0
10-11	34.433675985663086	39.0	35.0	39.0	25.0	39.0
12-13	34.28821304659498	39.0	35.0	39.0	24.0	39.0
14-15	35.4258517562724	40.0	36.0	41.0	25.0	41.0
16-17	35.34451842293907	40.0	36.0	41.0	24.0	41.0
18-19	35.35511541218638	40.0	36.0	41.0	24.0	41.0
20-21	35.37993835125448	40.0	36.0	41.0	24.0	41.0
22-23	35.33891125448029	40.0	35.0	41.0	24.0	41.0
24-25	35.29245505376344	40.0	35.0	41.0	24.0	41.0
26-27	35.21443555555555	40.0	35.0	41.0	24.0	41.0
28-29	35.16745146953405	40.0	35.0	41.0	24.0	41.0
30-31	35.072378781362005	40.0	35.0	41.0	24.0	41.0
32-33	34.96607971326165	40.0	35.0	41.0	24.0	41.0
34-35	34.80250982078853	39.5	34.5	41.0	23.0	41.0
36-37	34.65412788530466	39.0	34.0	41.0	22.0	41.0
38-39	34.516548530465954	39.0	34.0	41.0	20.5	41.0
40-41	34.36969663082438	39.0	34.0	41.0	19.5	41.0
42-43	34.16055397849462	39.0	33.0	41.0	17.5	41.0
44-45	33.94848630824373	39.0	33.0	41.0	15.5	41.0
46-47	33.685634408602155	38.0	33.0	40.5	14.5	41.0
48-49	33.533553835125446	38.0	33.0	40.0	12.5	41.0
50-51	33.331541218637994	38.0	33.0	40.0	9.0	41.0
52-53	33.12870164874552	38.0	33.0	40.0	8.0	41.0
54-55	32.917917419354836	37.5	32.0	40.0	7.5	41.0
56-57	32.673255627240145	37.0	32.0	40.0	7.0	41.0
58-59	32.41678394265233	36.5	32.0	40.0	4.0	41.0
60-61	32.10872229390681	36.0	31.5	39.5	2.0	41.0
62-63	31.79223340501792	35.5	31.0	39.0	2.0	41.0
64-65	31.47861304659498	35.0	31.0	39.0	2.0	40.5
66-67	31.43756788530466	35.0	31.0	39.0	2.0	40.0
68-69	31.191740501792115	35.0	31.0	38.0	2.0	40.0
70-71	30.89867670250896	35.0	31.0	37.0	2.0	40.0
72-73	30.574637706093192	35.0	31.0	37.0	2.0	39.0
74-75	30.283281433691755	35.0	30.5	36.0	2.0	39.0
76-77	30.013966738351257	35.0	30.0	36.0	2.0	38.0
78-79	29.750995842293907	35.0	30.0	35.0	2.0	37.0
80-81	29.520490896057346	35.0	30.0	35.0	2.0	37.0
82-83	29.33333419354839	35.0	30.0	35.0	2.0	36.0
84-85	29.145367455197132	34.0	30.0	35.0	2.0	36.0
86-87	29.00046365591398	34.0	30.0	35.0	2.0	36.0
88-89	28.882403727598565	34.0	29.5	35.0	2.0	35.0
90-91	28.772901792114695	34.0	29.0	35.0	2.0	35.0
92-93	28.676339498207884	34.0	29.0	35.0	2.0	35.0
94-95	28.613192258064515	34.0	29.0	35.0	2.0	35.0
96-97	28.514729175627238	34.0	29.0	35.0	2.0	35.0
98-99	28.458775627240144	34.0	29.0	35.0	2.0	35.0
100-101	28.396810322580645	34.0	29.0	35.0	2.0	35.0
102-103	27.41010752688172	32.5	26.5	34.5	2.0	35.0
104-105	28.24277046594982	34.0	29.0	35.0	2.0	35.0
106-107	28.57175025089606	34.0	29.5	35.0	2.0	35.0
108-109	28.613920860215053	35.0	30.0	35.0	2.0	35.0
110-111	28.555594838709677	35.0	30.0	35.0	2.0	35.0
112-113	28.483582508960573	34.5	30.0	35.0	2.0	35.0
114-115	28.36519512544803	34.0	29.0	35.0	2.0	35.0
116-117	28.305356559139785	34.0	29.0	35.0	2.0	35.0
118-119	28.14846795698925	34.0	29.0	35.0	2.0	35.0
120-121	27.977676559139784	34.0	29.0	35.0	2.0	35.0
122-123	27.848991254480286	34.0	29.0	35.0	2.0	35.0
124-125	26.992504946236558	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72316.0
3	12386.0
4	8098.0
5	7101.0
6	7570.0
7	8525.0
8	9289.0
9	9838.0
10	10218.0
11	10635.0
12	10778.0
13	11346.0
14	12183.0
15	10487.0
16	7829.0
17	6915.0
18	6291.0
19	5818.0
20	5558.0
21	5977.0
22	7121.0
23	9453.0
24	13830.0
25	18783.0
26	22297.0
27	23959.0
28	27736.0
29	33239.0
30	37565.0
31	45518.0
32	57192.0
33	74147.0
34	104634.0
35	165581.0
36	317585.0
37	437190.0
38	108663.0
39	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.12257144397899	16.673952478151172	12.098692340260017	25.10478373760982
2	16.825248226462154	18.875651291511858	38.34989132473683	25.94920915728916
3	19.6509095247847	23.5961321032334	28.342032487166378	28.41092588481552
4	13.73311993024243	14.78642480983031	34.8550924173063	36.62536284262096
5	14.820432623080038	36.56517513857225	33.087186641990805	15.527205596356907
6	34.19651632395812	33.92559306776122	16.989733947854447	14.888156660426214
7	30.312141965013616	30.75298576437117	20.10423282335669	18.830639447258523
8	28.159803475550042	33.20079100861631	19.061311199472662	19.578094316360996
9	27.79180042358589	14.750816309203929	18.55918844064864	38.89819482656154
10-11	26.7714744339956	24.555360117859255	26.40786852818204	22.265296919963102
12-13	26.997885956382433	22.119723137734102	26.59641846016161	24.28597244572186
14-15	24.382241696914207	23.181949347060467	24.026585708245634	28.409223247779696
16-17	24.701246189203673	26.271546704270733	24.413335291559623	24.613871814965975
18-19	24.755960485479843	25.409601383478698	25.931504263411775	23.902933867629685
20-21	25.82331695593942	24.367542231755618	25.721374277965808	24.08776653433916
22-23	25.67595520820393	24.003535741428394	25.548973777158203	24.771535273209473
24-25	25.091903958498317	24.494571267890137	25.54152463588472	24.87200013772682
26-27	24.73560050135854	24.548838994655064	25.736940536685438	24.978619967300958
28-29	24.81034759256268	24.58735199251575	25.708182443851612	24.894117971069956
30-31	25.05767076384177	24.538239424396863	25.54164329761155	24.862446514149813
32-33	24.6851711321158	24.65642317452621	25.556274413887387	25.1021312794706
34-35	24.981139154884975	24.689061398289986	25.610790181918947	24.71900926490609
36-37	25.159111043786808	24.426442843962157	25.454525111199857	24.95992100105118
38-39	25.060898596448745	24.634106312738467	25.486284976165624	24.818710114647168
40-41	25.099002799963277	24.55315340126687	25.54487400165244	24.802969797117417
42-43	25.021977189628142	24.55844784807163	25.41917329042713	25.000401671873103
44-45	24.955141472920484	24.6348627790998	25.395951456962496	25.014044291017218
46-47	25.092339752905623	24.650905091691303	25.33708708213267	24.919668073270408
48-49	25.176183567714016	24.83415865514955	25.003994880814844	24.985662896321585
50-51	25.053655256264275	25.0548314976795	24.951379630769214	24.940133615287007
52-53	25.193782658144897	24.904715397142553	25.01999800306885	24.881503941643704
54-55	25.095888540365564	24.81371660826813	25.125264715484285	24.96513013588202
56-57	25.012077225393732	24.93120858314926	25.057976419289137	24.998737772167875
58-59	25.11737425907768	24.75858167487029	24.988797593017274	25.135246473034762
60-61	25.056228280652338	24.893674694907254	25.241585836869863	24.808511187570545
62-63	24.90006199240313	24.91509393096955	25.18486559180656	24.999978484820755
64-65	25.019270086142377	24.73779917400393	25.168754892823326	25.07417584703037
66-67	24.929242421330507	24.90924929988219	25.131238922390832	25.030269356396467
68-69	25.02268116463729	24.91368536871987	25.06782837063045	24.99580509601239
70-71	24.876962115376617	24.76369984350413	25.22535548660748	25.133982554511768
72-73	25.07684995635823	24.92600404034146	25.044265632229106	24.952880371071203
74-75	24.87923231915255	25.086143787549858	25.023579817970692	25.011044075326904
76-77	24.98831135677181	24.82042515109185	24.979476750356397	25.211786741779946
78-79	24.94366455743404	24.921118906794103	25.05633544256596	25.0788810932059
80-81	24.962075767010266	25.09280645634074	24.929608206606115	25.01550957004288
82-83	25.029417846415754	24.96876101466404	24.96919120496015	25.032629933960056
84-85	24.893261944237235	25.0198820869638	24.880556910663536	25.20629905813542
86-87	24.78964737273949	25.086388043418722	25.04744208509434	25.07652249874745
88-89	24.900320820400804	24.9102442835403	24.954842506609427	25.234592389449467
90-91	24.814470897210082	24.949914741784898	24.975581106573056	25.260033254431963
92-93	24.87744370498155	25.088011380176177	24.97825348137887	25.056291433463397
94-95	24.89682270425319	24.854321487576996	25.04658070255486	25.20227510561495
96-97	24.872092012880703	24.85763864692406	25.067011712101568	25.203257628093674
98-99	24.76786001055312	24.947492486636534	24.953084493794307	25.331563009016033
100-101	24.790132732640586	24.970025106930287	25.070997695783152	25.16884446464597
102-103	24.804811779971466	24.77493171602469	25.181352201934175	25.23890430206967
104-105	24.62621704029908	24.991146321271394	25.048412058667413	25.33422457976211
106-107	24.66094321648418	24.970104732370682	25.02459011222268	25.34436193892246
108-109	24.7085969666267	24.932685863443428	25.024942579729505	25.33377459020037
110-111	24.62007377720477	24.998881642185143	25.118746659264474	25.262297921345606
112-113	24.65703928674151	24.986988154130223	25.11620331113123	25.239769247997035
114-115	24.508817117716998	25.181736376008907	25.07683828315832	25.232608223115776
116-117	24.421232173560952	25.244176189968215	25.093741863183332	25.2408497732875
118-119	24.701949732624932	24.96445298409546	24.980088784905703	25.353508498373905
120-121	24.428183403743596	25.015100245776463	25.18703729868617	25.36967905179377
122-123	24.410507540151567	25.13003351975176	25.080959937773205	25.37849900232347
124-125	24.457002662727152	25.17957493859326	25.035226796337216	25.328195602342372
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	96.5
2	115.0
3	127.0
4	128.0
5	125.5
6	135.0
7	170.5
8	228.0
9	283.0
10	328.5
11	376.5
12	439.5
13	517.5
14	595.5
15	673.5
16	732.0
17	801.5
18	885.0
19	985.5
20	1160.5
21	1333.0
22	1531.0
23	1757.5
24	2049.0
25	2603.0
26	3410.0
27	4312.0
28	5135.5
29	6414.0
30	8190.5
31	9716.5
32	11649.5
33	14007.5
34	16268.0
35	18549.5
36	21738.0
37	25136.0
38	27962.5
39	31142.0
40	34337.0
41	37131.0
42	39904.0
43	44522.5
44	49591.5
45	53889.5
46	58544.5
47	62692.0
48	67312.0
49	71858.0
50	73123.5
51	74157.0
52	74866.0
53	75088.5
54	76244.5
55	75806.5
56	72579.5
57	67241.0
58	63096.5
59	58710.0
60	52864.5
61	46916.0
62	40437.5
63	32285.0
64	24683.0
65	19608.5
66	15372.5
67	11941.0
68	9538.0
69	7752.0
70	6529.0
71	5246.5
72	4179.5
73	3677.0
74	3047.5
75	2355.5
76	1702.0
77	1090.0
78	696.0
79	461.5
80	267.0
81	131.5
82	73.0
83	46.0
84	32.0
85	21.5
86	18.5
87	18.5
88	15.5
89	15.5
90	15.0
91	12.0
92	17.5
93	14.0
94	10.5
95	15.5
96	10.0
97	6.5
98	8.0
99	8.5
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036473118279569894
2	0.027698924731182798
3	0.02735483870967742
4	0.03268817204301075
5	0.03532616487455197
6	0.04708243727598566
7	0.04180645161290322
8	0.0377921146953405
9	0.030853046594982076
10-11	0.03750537634408602
12-13	0.036989247311827955
14-15	0.0363010752688172
16-17	0.038767025089605736
18-19	0.05144086021505376
20-21	0.06331182795698925
22-23	0.055942652329749105
24-25	0.06709677419354838
26-27	0.050781362007168464
28-29	0.05106810035842294
30-31	0.05060931899641577
32-33	0.058351254480286746
34-35	0.04134767025089606
36-37	0.05402150537634409
38-39	0.06302508960573476
40-41	0.04989247311827957
42-43	0.05929749103942652
44-45	0.06015770609318997
46-47	0.06517562724014338
48-49	0.05129749103942652
50-51	0.05221505376344086
52-53	0.06170609318996415
54-55	0.048229390681003584
56-57	0.045878136200716846
58-59	0.04708243727598566
60-61	0.03627240143369175
62-63	0.04556272401433692
64-65	0.04378494623655914
66-67	0.03730465949820788
68-69	0.03231541218637993
70-71	0.051182795698924734
72-73	0.03329032258064516
74-75	0.0420931899641577
76-77	0.03469534050179211
78-79	0.035555555555555556
80-81	0.027039426523297494
82-83	0.01924014336917563
84-85	0.019870967741935485
86-87	0.017691756272401434
88-89	0.023340501792114696
90-91	0.01278853046594982
92-93	0.021075268817204302
94-95	0.015512544802867383
96-97	0.012129032258064516
98-99	0.010896057347670251
100-101	0.011899641577060931
102-103	0.0064516129032258064
104-105	0.007139784946236559
106-107	0.009347670250896058
108-109	0.014078853046594982
110-111	0.006996415770609319
112-113	0.008401433691756272
114-115	0.008774193548387096
116-117	0.00756989247311828
118-119	0.23813620071684588
120-121	0.35529749103942654
122-123	0.025777777777777778
124-125	0.002810035842293907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1743750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.6246541522177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03325939416854	46.91922152657858
2	10.157859984086011	11.910020569873879
3	3.509093988307684	6.1715826435649275
4	1.804984334702187	4.232663294883459
5	1.0544923296386586	3.0909624065616357
6	0.7167131680733505	2.521023696278427
7	0.4912452728531135	2.015935895744792
8	0.365012109702174	1.711896695412904
9	0.2743346843844282	1.447449839459541
>10	1.5069513536671106	15.385788957987092
>50	0.06684941028238357	2.62936925308759
>100	0.018810132367654928	1.7367202038425598
>500	1.969188833611173E-4	0.08092555304593566
>1k	1.969188833611173E-4	0.14643946367870095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.7347670250896056E-5
10-11	0.0	0.0	0.0	0.0	5.7347670250896056E-5
12-13	0.0	0.0	0.0	0.0	2.867383512544803E-4
14-15	0.0	0.0	0.0	0.0	3.154121863799283E-4
16-17	0.0	0.0	0.0	0.0	3.440860215053763E-4
18-19	0.0	0.0	0.0	0.0	3.440860215053763E-4
20-21	0.0	0.0	0.0	0.0	4.3010752688172043E-4
22-23	0.0	0.0	0.0	0.0	4.8745519713261647E-4
24-25	0.0	0.0	0.0	8.602150537634408E-5	6.308243727598566E-4
26-27	0.0	0.0	0.0	2.0071684587813618E-4	6.308243727598566E-4
28-29	0.0	0.0	0.0	9.46236559139785E-4	6.308243727598566E-4
30-31	0.0	0.0	0.0	0.0027240143369175627	6.308243727598566E-4
32-33	0.0	0.0	0.0	0.0062795698924731184	6.308243727598566E-4
34-35	0.0	0.0	0.0	0.012903225806451613	7.455197132616488E-4
36-37	0.0	0.0	0.0	0.022480286738351257	7.455197132616488E-4
38-39	0.0	0.0	0.0	0.03845161290322581	7.455197132616488E-4
40-41	0.0	0.0	0.0	0.056602150537634406	7.455197132616488E-4
42-43	0.0	0.0	0.0	0.07317562724014337	7.741935483870969E-4
44-45	0.0	0.0	0.0	0.09488172043010754	8.028673835125448E-4
46-47	0.0	0.0	0.0	0.12048745519713262	8.315412186379928E-4
48-49	0.0	0.0	0.0	0.1454336917562724	9.175627240143369E-4
50-51	0.0	0.0	0.0	0.17410752688172043	9.175627240143369E-4
52-53	0.0	0.0	0.0	0.20237992831541218	9.749103942652329E-4
54-55	0.0	0.0	0.0	0.2341505376344086	0.001032258064516129
56-57	0.0	0.0	0.0	0.27303225806451614	0.001032258064516129
58-59	0.0	0.0	0.0	0.31372043010752687	0.001032258064516129
60-61	0.0	0.0	0.0	0.35848028673835125	0.001032258064516129
62-63	0.0	0.0	0.0	0.4073405017921147	0.001089605734767025
64-65	0.0	0.0	0.0	0.46015770609318996	0.001118279569892473
66-67	0.0	0.0	0.0	0.5179641577060932	0.0011469534050179211
68-69	0.0	0.0	0.0	0.5864659498207885	0.0011469534050179211
70-71	0.0	0.0	0.0	0.6595268817204301	0.0012329749103942651
72-73	0.0	0.0	0.0	0.7370322580645161	0.0014336917562724014
74-75	0.0	0.0	0.0	0.8196989247311828	0.0014336917562724014
76-77	0.0	0.0	0.0	0.9108243727598566	0.0014336917562724014
78-79	0.0	0.0	0.0	1.0106379928315412	0.0014336917562724014
80-81	0.0	0.0	0.0	1.121921146953405	0.0014623655913978495
82-83	0.0	0.0	0.0	1.2434695340501793	0.0015770609318996416
84-85	0.0	0.0	0.0	1.3691469534050178	0.0016057347670250896
86-87	0.0	0.0	0.0	1.508616487455197	0.0016057347670250896
88-89	0.0	0.0	0.0	1.6608172043010754	0.0016057347670250896
90-91	0.0	0.0	0.0	1.837706093189964	0.0016344086021505377
92-93	0.0	0.0	0.0	2.0242293906810036	0.0017777777777777779
94-95	0.0	0.0	0.0	2.217978494623656	0.0018351254480286738
96-97	0.0	0.0	0.0	2.42431541218638	0.0018924731182795697
98-99	0.0	0.0	0.0	2.6518709677419356	0.0019498207885304659
100-101	0.0	0.0	0.0	2.9058924731182794	0.0019498207885304659
102-103	0.0	0.0	0.0	3.1790967741935483	0.002007168458781362
104-105	0.0	0.0	0.0	3.469878136200717	0.002007168458781362
106-107	0.0	0.0	0.0	3.76510394265233	0.00203584229390681
108-109	0.0	0.0	0.0	4.077849462365592	0.0021218637992831543
110-111	0.0	0.0	0.0	4.423197132616487	0.002207885304659498
112-113	0.0	0.0	0.0	4.797476702508961	0.002236559139784946
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1845	0.0	67.748055	1
GTATCAA	3630	0.0	62.309013	1
TCAACGC	4805	0.0	45.820263	4
CAACGCA	4860	0.0	45.179283	5
AACGCAG	5170	0.0	42.923264	6
ATCAACG	5215	0.0	42.215477	3
TATCAAC	5355	0.0	41.111805	2
GTACATG	8920	0.0	37.7014	1
ACGCAGA	6165	0.0	35.801632	7
TACATGG	9330	0.0	35.58581	2
CGCAGAG	6500	0.0	34.134624	8
ACATGGG	9510	0.0	33.911194	3
CATGGGG	8215	0.0	31.653599	4
GCAGAGT	7105	0.0	30.2251	9
TACCGTA	235	3.4197E-10	27.847538	7
ATGGGGG	4425	0.0	27.835966	5
AGAGTAC	6350	0.0	27.308756	10-11
CAGAGTA	6820	0.0	24.816183	10-11
TATATAG	435	0.0	24.622519	5
GTATAGG	550	0.0	22.726393	1
>>END_MODULE