FastQCFastQC Report
Mon 15 Aug 2016
SRR2921487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743750
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.15168458781362007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.1393548387096774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.079.964041
GTATCAA51150.061.0793651
TCAACGC69100.044.5474134
TATCAAC71750.043.3182682
CAACGCA71400.043.0876775
ATCAACG72200.042.7184033
AACGCAG73850.041.6486666
ACGCAGA86850.035.274527
CGCAGAG91750.033.389698
GTACATG87200.033.093121
TACATGG90500.031.8433862
GCAGAGT96800.031.0947029
GTGGTAT9850.030.2651351
ACATGGG94550.029.5347823
CATGGGG81500.028.8568884
CAGAGTA93400.026.78160510-11
AGAGTAC89000.026.66860810-11
GAGTACT48450.026.58200812-13
GTACTTT52450.024.49877714-15
GTATAGG5500.023.8489271
Produced by FastQC (version 0.11.5)