##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1743750
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09690322580645	31.0	31.0	34.0	28.0	34.0
2	31.16830910394265	33.0	31.0	34.0	27.0	34.0
3	31.245407311827957	33.0	31.0	34.0	27.0	34.0
4	34.757990537634406	37.0	35.0	37.0	32.0	37.0
5	34.39362523297491	37.0	35.0	37.0	30.0	37.0
6	34.37219211469534	37.0	35.0	37.0	30.0	37.0
7	34.20874322580645	37.0	35.0	37.0	30.0	37.0
8	34.17705691756272	37.0	35.0	37.0	30.0	37.0
9	35.73306035842294	39.0	37.0	39.0	30.0	39.0
10-11	35.59788673835125	39.0	35.0	39.0	30.0	39.0
12-13	35.49959655913979	39.0	35.0	39.0	30.0	39.0
14-15	36.658859068100355	40.0	36.5	41.0	29.5	41.0
16-17	36.6278746953405	40.0	36.0	41.0	29.0	41.0
18-19	36.64240516129033	40.0	36.5	41.0	29.0	41.0
20-21	36.61942910394265	40.0	37.0	41.0	29.0	41.0
22-23	36.55725189964158	40.0	36.5	41.0	29.0	41.0
24-25	36.481199713261645	40.0	36.0	41.0	29.0	41.0
26-27	36.35359856630824	40.0	36.0	41.0	28.5	41.0
28-29	36.163549534050176	40.0	36.0	41.0	27.5	41.0
30-31	36.115686308243724	40.0	36.0	41.0	28.0	41.0
32-33	35.991106810035845	40.0	35.0	41.0	27.5	41.0
34-35	35.867873548387095	40.0	35.0	41.0	27.0	41.0
36-37	35.72730207885304	40.0	35.0	41.0	27.0	41.0
38-39	35.55109161290322	39.5	35.0	41.0	26.0	41.0
40-41	35.339639569892476	39.0	35.0	41.0	25.5	41.0
42-43	35.16469247311828	39.0	35.0	41.0	25.0	41.0
44-45	34.939441146953406	39.0	35.0	41.0	24.0	41.0
46-47	34.65371498207885	39.0	34.0	41.0	23.0	41.0
48-49	34.38154752688172	39.0	34.0	41.0	22.0	41.0
50-51	34.159039139784944	38.5	34.0	41.0	20.5	41.0
52-53	33.95083784946236	38.0	33.0	40.0	20.0	41.0
54-55	33.71609204301075	38.0	33.0	40.0	19.5	41.0
56-57	33.445551254480286	37.0	33.0	40.0	18.0	41.0
58-59	33.294776200716846	37.0	33.0	40.0	18.0	41.0
60-61	33.167488458781364	36.5	33.0	40.0	18.0	41.0
62-63	32.892651469534044	36.0	33.0	39.5	17.5	41.0
64-65	32.590856774193554	35.5	32.5	39.0	15.0	41.0
66-67	32.29096516129032	35.0	32.0	39.0	13.0	41.0
68-69	31.974704802867382	35.0	32.0	38.5	12.5	40.5
70-71	31.67796215053763	35.0	32.0	37.0	10.5	40.0
72-73	31.372056487455197	35.0	32.0	37.0	8.0	39.0
74-75	31.087525734767027	35.0	31.5	36.0	7.5	39.0
76-77	30.807980788530465	35.0	31.0	36.0	7.0	38.5
78-79	30.54224917562724	35.0	31.0	35.5	6.5	37.0
80-81	30.330739211469535	35.0	31.0	35.0	2.0	37.0
82-83	30.158807741935483	35.0	31.0	35.0	2.0	36.0
84-85	29.978049032258063	35.0	31.0	35.0	2.0	36.0
86-87	29.840895770609322	35.0	31.0	35.0	2.0	36.0
88-89	29.742947096774195	35.0	31.0	35.0	2.0	35.5
90-91	29.648720860215054	35.0	31.0	35.0	2.0	35.0
92-93	29.714811469534048	35.0	31.0	35.0	2.0	35.0
94-95	29.688714265232974	35.0	31.0	35.0	2.0	35.0
96-97	29.673076702508958	35.0	31.0	35.0	2.0	35.0
98-99	29.633249318996413	35.0	31.0	35.0	2.0	35.0
100-101	29.58511111111111	35.0	31.0	35.0	2.0	35.0
102-103	28.696299641577063	33.5	29.0	35.0	2.0	35.0
104-105	29.4101941218638	34.5	30.5	35.0	2.0	35.0
106-107	29.520936487455195	35.0	31.0	35.0	2.0	35.0
108-109	29.52570007168459	35.0	31.0	35.0	2.0	35.0
110-111	29.492345806451613	35.0	31.0	35.0	2.0	35.0
112-113	29.448721146953403	35.0	31.0	35.0	2.0	35.0
114-115	29.380416917562727	35.0	31.0	35.0	2.0	35.0
116-117	29.3045135483871	35.0	31.0	35.0	2.0	35.0
118-119	29.208140788530464	35.0	31.0	35.0	2.0	35.0
120-121	29.087183082437278	35.0	31.0	35.0	2.0	35.0
122-123	28.96452329749104	35.0	31.0	35.0	2.0	35.0
124-125	28.043639856630826	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33546.0
3	12102.0
4	6708.0
5	5699.0
6	6048.0
7	6881.0
8	7864.0
9	8746.0
10	9714.0
11	10520.0
12	11007.0
13	12276.0
14	15520.0
15	12967.0
16	7568.0
17	6418.0
18	5696.0
19	5167.0
20	5106.0
21	5391.0
22	6330.0
23	8515.0
24	12118.0
25	16601.0
26	20517.0
27	22766.0
28	26894.0
29	31720.0
30	36658.0
31	44004.0
32	55626.0
33	70991.0
34	99227.0
35	158338.0
36	318055.0
37	483292.0
38	136911.0
39	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00513584879877	17.26994469130075	12.516542945054743	25.20837651484573
2	17.141456197095863	19.380061018970935	37.23086275319432	26.247620030738876
3	19.767969434280662	23.67552660303971	28.572420070999566	27.98408389168007
4	13.880793729211028	15.332069857259336	34.456863079243824	36.33027333428582
5	14.768726857627984	36.643176531662924	32.946592209535446	15.641504401173648
6	34.37664194052418	34.208002459612565	16.32103221128806	15.094323388575193
7	30.023482014520535	31.158809311588094	19.855207627123505	18.96250104676787
8	28.16414243372455	33.24382667902401	18.786475586591667	19.805555300659773
9	27.830231151052526	14.37196938632134	18.386043190635814	39.41175627199031
10-11	26.885718447860352	24.497908357736783	26.13420118994773	22.482172004455133
12-13	27.19501413844116	22.10680982226656	26.647542038957923	24.050634000334355
14-15	24.52023562613749	23.06424663265268	24.240341413017017	28.175176328192812
16-17	24.51492421748372	26.156496373474226	24.654037585558594	24.674541823483462
18-19	24.55671984441823	24.913041558249397	26.72762694785476	23.80261164947762
20-21	25.64652366452406	23.891995317510524	26.6289631818904	23.832517836075017
22-23	25.46048880981795	23.557538446714865	26.47239992154083	24.509572821926355
24-25	24.8115638956338	24.12899832549037	26.50878651536525	24.550651263510574
26-27	24.543365835557214	24.13466485200218	26.695699004286027	24.626270308154577
28-29	24.71528240612751	24.175729947312334	26.60753021826437	24.50145742829579
30-31	24.86090807901861	24.141636717341754	26.468664129613966	24.52879107402567
32-33	24.514484590836403	24.180474078696513	26.488550669110783	24.8164906613563
34-35	24.708861369399106	24.269339190952238	26.508995075917596	24.51280436373106
36-37	24.977501905670323	24.098427226909845	26.27395996859157	24.650110898828263
38-39	24.853335994867372	24.344403651464088	26.197984289492304	24.60427606417624
40-41	24.926701438555963	24.195608509974452	26.29646113627682	24.581228915192764
42-43	24.95541499541604	24.24561082531654	26.042804472064507	24.756169707202915
44-45	24.88145447203576	24.342600734302575	25.96727816485156	24.808666628810105
46-47	25.073246248495785	24.433560433030443	25.644647288053346	24.848546030420422
48-49	25.1677300877075	24.626445097273844	25.19589423016529	25.009930584853368
50-51	25.033216889006404	24.94437085219065	25.10697802738282	24.91543423142012
52-53	25.250459102124445	24.829172136265353	25.098223332856286	24.82214542875392
54-55	25.117849617664493	24.766157736805532	25.181058368003985	24.93493427752599
56-57	24.996522504771697	24.86748950656852	25.150994993840893	24.984992994818892
58-59	25.023832084467102	24.653273283745772	25.250251226305576	25.072643405481553
60-61	25.07502727482353	24.768981384287635	25.426846686238	24.72914465465083
62-63	24.91691979587523	24.855285426784306	25.330406393913314	24.897388383427153
64-65	24.996816583591926	24.596709896123116	25.440573359106576	24.965900161178382
66-67	24.973573681394093	24.768730280632305	25.325331927753343	24.93236411022026
68-69	24.985623534209967	24.804759707233494	25.243811953246155	24.965804805310388
70-71	24.83932876274687	24.560258381718725	25.5027195811564	25.09769327437801
72-73	25.176384483040966	24.799654013673685	25.10916034934753	24.91480115393782
74-75	24.910763092429534	25.050605342570492	25.04369353324236	24.994938031757613
76-77	25.105707396420517	24.67567961309831	25.04754825373446	25.17106473674671
78-79	24.953089388593106	24.860673619743817	25.07131961801482	25.114917373648254
80-81	24.94845449526418	24.956370232811594	24.963855767231426	25.1313195046928
82-83	25.026217767631287	24.862587926664236	24.985554060219567	25.12564024548491
84-85	24.884126955904293	24.98619159244349	24.83967621506817	25.29000523658405
86-87	24.786673820794825	24.98259981055517	25.09564727127462	25.13507909737539
88-89	24.872380315407543	24.84980900426102	24.919530551144383	25.358280129187055
90-91	24.747263575441806	24.870090093810436	25.04244297155862	25.340203359189132
92-93	24.769134725870874	24.980708056619523	25.028597384669165	25.221559832840434
94-95	24.822021450910153	24.772669790073348	25.04583883198584	25.35946992703066
96-97	24.7387812650273	24.763986267144922	25.103407894069026	25.393824573758756
98-99	24.73019584296433	24.7528485819242	24.96317496201365	25.553780613097814
100-101	24.671243561948682	24.823204514952337	25.093315897541785	25.4122360255572
102-103	24.667406670992758	24.616021198801274	25.226969229220785	25.489602900985187
104-105	24.524272278956722	24.8735875556361	25.076346406514432	25.525793758892746
106-107	24.58953921782558	24.812479372659308	24.994673809162656	25.60330760035246
108-109	24.63543042397534	24.778743041938657	24.964845819197812	25.62098071488819
110-111	24.598670728060117	24.770485678312063	25.06147000994703	25.569373583680793
112-113	24.5759285238252	24.754527195289675	25.088639770953225	25.5809045099319
114-115	24.33301351550524	24.939338797190576	25.070611303199343	25.657036384104842
116-117	24.340495428855405	24.977711350736595	24.986773059539725	25.69502016086827
118-119	24.38252212636814	24.706169459174006	25.04736704607142	25.863941368386428
120-121	24.198782513594136	24.80190540149552	25.152142788117853	25.847169296792494
122-123	24.191786784543666	24.811002337793195	25.127878979560297	25.869331898102843
124-125	24.19639377070912	24.908473339606715	24.992507218166	25.902625671518166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	21.0
2	42.0
3	68.5
4	63.0
5	57.0
6	96.0
7	194.0
8	330.5
9	412.0
10	392.5
11	368.5
12	438.0
13	543.5
14	648.5
15	795.5
16	985.0
17	1133.5
18	1329.5
19	1555.0
20	1720.5
21	1946.0
22	2231.0
23	2486.0
24	2747.5
25	3274.0
26	4140.0
27	5024.5
28	5678.5
29	6771.5
30	8475.5
31	10137.0
32	11931.0
33	13808.5
34	15948.5
35	18240.5
36	21104.5
37	24184.5
38	26898.0
39	30250.5
40	33585.5
41	36020.5
42	38790.5
43	43723.0
44	48952.0
45	53364.5
46	57800.0
47	62014.0
48	67278.0
49	72142.5
50	73324.0
51	74473.5
52	75078.5
53	75478.0
54	77249.5
55	76750.5
56	73219.5
57	67545.0
58	63360.0
59	59225.0
60	53226.0
61	46943.0
62	40097.5
63	31911.0
64	24563.0
65	19294.0
66	14944.0
67	11672.5
68	9335.5
69	7539.0
70	6315.0
71	5169.5
72	4140.0
73	3604.0
74	2921.0
75	2296.5
76	1646.0
77	946.0
78	562.0
79	370.5
80	205.5
81	83.5
82	52.5
83	24.0
84	8.5
85	5.0
86	2.5
87	3.0
88	4.5
89	2.0
90	2.0
91	2.5
92	1.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11876702508960574
2	0.0017204301075268817
3	0.020587813620071685
4	0.0538494623655914
5	0.02666666666666667
6	0.02213620071684588
7	0.01651612903225806
8	0.006767025089605735
9	0.004874551971326165
10-11	0.00825806451612903
12-13	0.005706093189964158
14-15	0.0031827956989247314
16-17	0.011928315412186379
18-19	0.005792114695340502
20-21	0.01336200716845878
22-23	0.009634408602150537
24-25	0.014594982078853047
26-27	0.010150537634408601
28-29	0.015369175627240144
30-31	0.02227956989247312
32-33	0.013878136200716845
34-35	0.021763440860215054
36-37	0.015426523297491038
38-39	0.022623655913978497
40-41	0.011240143369175629
42-43	0.009777777777777778
44-45	0.01875268817204301
46-47	0.018121863799283155
48-49	0.02285304659498208
50-51	0.01631541218637993
52-53	0.023025089605734766
54-55	0.018379928315412186
56-57	0.022795698924731184
58-59	0.01731899641577061
60-61	0.022021505376344085
62-63	0.023197132616487454
64-65	0.01949820788530466
66-67	0.01278853046594982
68-69	0.025777777777777778
70-71	0.016229390681003583
72-73	0.018838709677419355
74-75	0.020473118279569893
76-77	0.014795698924731183
78-79	0.031025089605734767
80-81	0.022222222222222223
82-83	0.010351254480286738
84-85	0.014164874551971325
86-87	0.013448028673835124
88-89	0.022164874551971324
90-91	0.013419354838709678
92-93	0.008401433691756272
94-95	0.008086021505376345
96-97	0.002781362007168459
98-99	0.001806451612903226
100-101	0.012043010752688172
102-103	0.0038136200716845877
104-105	0.003039426523297491
106-107	4.8745519713261647E-4
108-109	0.020559139784946237
110-111	7.741935483870968E-4
112-113	0.010093189964157706
114-115	0.002810035842293907
116-117	0.00857347670250896
118-119	0.010637992831541218
120-121	0.004329749103942652
122-123	6.594982078853047E-4
124-125	0.023254480286738353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1743750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.15244214361226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17348318328052	45.24957916940698
2	10.562162165348935	12.073067241331106
3	3.741207410926073	6.414574203006174
4	1.8558646063353341	4.242687781598317
5	1.0876144302011554	3.107991039831467
6	0.732809560426862	2.5129113602749293
7	0.5015900850006306	2.0066968818965667
8	0.37887068062309726	1.7322707723378052
9	0.28571819001975496	1.4696543092033494
>10	1.5866824758285925	15.978375000521233
>50	0.07223375405183714	2.783767895312915
>100	0.021259779411320102	1.996512315176064
>500	2.0147141834338288E-4	0.07069954318403479
>1k	3.022071275150744E-4	0.3612124869190833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2645	0.15168458781362007	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2430	0.1393548387096774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1469534050179211E-4
7	0.0	0.0	0.0	0.0	1.1469534050179211E-4
8	0.0	0.0	0.0	0.0	1.1469534050179211E-4
9	0.0	0.0	0.0	0.0	1.1469534050179211E-4
10-11	0.0	0.0	0.0	0.0	1.1469534050179211E-4
12-13	0.0	0.0	0.0	5.7347670250896056E-5	2.867383512544803E-4
14-15	0.0	0.0	0.0	5.7347670250896056E-5	3.154121863799283E-4
16-17	0.0	0.0	0.0	1.1469534050179211E-4	3.440860215053763E-4
18-19	0.0	0.0	0.0	1.7204301075268816E-4	3.440860215053763E-4
20-21	0.0	0.0	0.0	1.7204301075268816E-4	4.014336917562724E-4
22-23	0.0	0.0	0.0	2.0071684587813618E-4	4.8745519713261647E-4
24-25	0.0	0.0	0.0	2.867383512544803E-4	5.161290322580645E-4
26-27	0.0	0.0	0.0	3.727598566308244E-4	5.161290322580645E-4
28-29	0.0	0.0	0.0	0.0011756272401433692	5.161290322580645E-4
30-31	0.0	0.0	0.0	0.002924731182795699	5.448028673835125E-4
32-33	0.0	0.0	0.0	0.006566308243727599	6.881720430107526E-4
34-35	0.0	0.0	0.0	0.01336200716845878	6.881720430107526E-4
36-37	0.0	0.0	0.0	0.023168458781362006	6.881720430107526E-4
38-39	0.0	0.0	0.0	0.039197132616487454	7.455197132616488E-4
40-41	0.0	0.0	0.0	0.05794982078853046	8.028673835125448E-4
42-43	5.7347670250896056E-5	0.0	0.0	0.07489605734767026	8.028673835125448E-4
44-45	5.7347670250896056E-5	0.0	0.0	0.09723297491039426	8.315412186379928E-4
46-47	5.7347670250896056E-5	0.0	0.0	0.1236989247311828	8.602150537634409E-4
48-49	5.7347670250896056E-5	0.0	0.0	0.1489605734767025	9.175627240143369E-4
50-51	5.7347670250896056E-5	0.0	0.0	0.177921146953405	9.175627240143369E-4
52-53	5.7347670250896056E-5	0.0	0.0	0.20685304659498208	0.0010609318996415771
54-55	5.7347670250896056E-5	0.0	0.0	0.23870967741935484	0.001089605734767025
56-57	5.7347670250896056E-5	0.0	0.0	0.27741935483870966	0.001089605734767025
58-59	5.7347670250896056E-5	0.0	0.0	0.3193978494623656	0.001089605734767025
60-61	5.7347670250896056E-5	0.0	0.0	0.3651039426523297	0.001089605734767025
62-63	5.7347670250896056E-5	0.0	0.0	0.41439426523297496	0.0013189964157706094
64-65	5.7347670250896056E-5	0.0	0.0	0.46818637992831547	0.0013763440860215053
66-67	5.7347670250896056E-5	0.0	0.0	0.5266236559139785	0.0014336917562724014
68-69	5.7347670250896056E-5	0.0	0.0	0.595899641577061	0.0014910394265232976
70-71	5.7347670250896056E-5	0.0	0.0	0.6702222222222223	0.0016630824372759856
72-73	5.7347670250896056E-5	0.0	0.0	0.7483584229390681	0.0016630824372759856
74-75	5.7347670250896056E-5	0.0	0.0	0.8319426523297491	0.0016630824372759856
76-77	5.7347670250896056E-5	0.0	0.0	0.9234695340501793	0.0016630824372759856
78-79	5.7347670250896056E-5	0.0	0.0	1.0259498207885305	0.0016630824372759856
80-81	5.7347670250896056E-5	0.0	0.0	1.1391541218637993	0.0016630824372759856
82-83	5.7347670250896056E-5	0.0	0.0	1.2618494623655914	0.0016630824372759856
84-85	5.7347670250896056E-5	0.0	0.0	1.390336917562724	0.0016630824372759856
86-87	5.7347670250896056E-5	0.0	0.0	1.5324444444444445	0.0017777777777777779
88-89	1.1469534050179211E-4	0.0	0.0	1.6867956989247312	0.0019498207885304659
90-91	1.1469534050179211E-4	0.0	0.0	1.8664659498207885	0.0019498207885304659
92-93	1.1469534050179211E-4	0.0	0.0	2.056573476702509	0.0020931899641577064
94-95	1.1469534050179211E-4	0.0	0.0	2.2544229390681005	0.00217921146953405
96-97	1.1469534050179211E-4	0.0	0.0	2.466035842293907	0.0023799283154121863
98-99	1.1469534050179211E-4	0.0	0.0	2.6982939068100356	0.0024659498207885303
100-101	1.1469534050179211E-4	0.0	0.0	2.956272401433692	0.0024659498207885303
102-103	1.1469534050179211E-4	0.0	0.0	3.2321146953405018	0.002494623655913978
104-105	1.1469534050179211E-4	0.0	0.0	3.5272258064516127	0.0025519713261648747
106-107	1.1469534050179211E-4	0.0	0.0	3.8265806451612905	0.0025806451612903226
108-109	1.1469534050179211E-4	0.0	0.0	4.145060931899641	0.0026379928315412187
110-111	1.1469534050179211E-4	0.0	0.0	4.497605734767025	0.002867383512544803
112-113	1.1469534050179211E-4	5.7347670250896056E-5	0.0	4.883555555555556	0.002924731182795699
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2550	0.0	79.96404	1
GTATCAA	5115	0.0	61.079365	1
TCAACGC	6910	0.0	44.547413	4
TATCAAC	7175	0.0	43.318268	2
CAACGCA	7140	0.0	43.087677	5
ATCAACG	7220	0.0	42.718403	3
AACGCAG	7385	0.0	41.648666	6
ACGCAGA	8685	0.0	35.27452	7
CGCAGAG	9175	0.0	33.38969	8
GTACATG	8720	0.0	33.09312	1
TACATGG	9050	0.0	31.843386	2
GCAGAGT	9680	0.0	31.094702	9
GTGGTAT	985	0.0	30.265135	1
ACATGGG	9455	0.0	29.534782	3
CATGGGG	8150	0.0	28.856888	4
CAGAGTA	9340	0.0	26.781605	10-11
AGAGTAC	8900	0.0	26.668608	10-11
GAGTACT	4845	0.0	26.582008	12-13
GTACTTT	5245	0.0	24.498777	14-15
GTATAGG	550	0.0	23.848927	1
>>END_MODULE